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2-haloacid dehalogenase, configuration-inverting (EC 3.8.1.10) (DL-2-haloacid dehalogenase) (DL-DEXi)

 HADDL_PSES4             Reviewed;         307 AA.
O06652;
01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
01-JUL-1997, sequence version 1.
15-MAR-2017, entry version 44.
RecName: Full=2-haloacid dehalogenase, configuration-inverting;
EC=3.8.1.10;
AltName: Full=DL-2-haloacid dehalogenase;
AltName: Full=DL-DEXi;
Pseudomonas sp. (strain 113).
Bacteria; Proteobacteria.
NCBI_TaxID=59918;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS.
PubMed=9209038; DOI=10.1128/jb.179.13.4232-4238.1997;
Nardi-Dei V., Kurihara T., Park C., Esaki N., Soda K.;
"Bacterial DL-2-haloacid dehalogenase from Pseudomonas sp. strain 113:
gene cloning and structural comparison with D- and L-2-haloacid
dehalogenases.";
J. Bacteriol. 179:4232-4238(1997).
-!- FUNCTION: Dehalogenates both (S)- and (R)-2-haloalkanoic acids to
the corresponding (R)- and (S)-hydroxyalkanoic acids,
respectively, with inversion of configuration at C-2. Acts on 2-
haloalkanoic acids whose carbon chain lengths are five or less.
-!- CATALYTIC ACTIVITY: (S)-2-haloacid + H(2)O = (R)-2-hydroxyacid +
halide.
-!- CATALYTIC ACTIVITY: (R)-2-haloacid + H(2)O = (S)-2-hydroxyacid +
halide.
-!- SUBUNIT: Homodimer.
-!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. S-2-
haloalkanoic acid dehalogenase family. {ECO:0000305}.
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EMBL; U97030; AAB62819.1; -; Genomic_DNA.
ProteinModelPortal; O06652; -.
SMR; O06652; -.
BRENDA; 3.8.1.10; 5085.
GO; GO:0033976; F:2-haloacid dehalogenase (configuration-inverting) activity; IEA:UniProtKB-EC.
InterPro; IPR019714; 2-haloacid_dehalogenase_DehI.
Pfam; PF10778; DehI; 1.
1: Evidence at protein level;
Hydrolase.
CHAIN 1 307 2-haloacid dehalogenase, configuration-
inverting.
/FTId=PRO_0000079172.
MUTAGEN 26 26 Y->F: 60% activity of wild-type.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 28 28 D->N: Less than 10% activity.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 34 34 R->A: Almost no effect.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 37 37 W->F: 40% activity of wild-type.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 43 43 R->A: Almost no effect.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 65 65 T->A: Complete loss of activity.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 69 69 E->D,N,Q: Complete loss of activity.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 73 73 D->N: 50% activity of wild-type.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 76 76 R->A: Almost no effect.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 102 102 E->Q: 15% activity of wild-type.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 115 115 Y->F: Almost no effect.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 117 117 N->D: Less than 10% activity.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 119 119 K->R: Almost no effect.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 120 120 Y->F: Less than 10% activity.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 125 125 S->A: 60% activity of wild-type.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 129 129 E->Q: No effect.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 134 134 R->A: 50% activity of wild-type.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 161 161 E->Q: No effect.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 167 167 D->N: No effect.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 193 193 S->A: No effect.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 194 194 D->A,E,G,N,S: Complete loss of activity.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 201 201 W->F: 60% activity of wild-type.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 219 219 T->A: Less than 10% activity.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 250 250 D->N: Less than 10% activity.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 256 256 T->A: Almost no effect.
{ECO:0000269|PubMed:9209038}.
MUTAGEN 271 271 N->D: Almost no effect.
{ECO:0000269|PubMed:9209038}.
SEQUENCE 307 AA; 34239 MW; 4CAD158995156ABD CRC64;
MSHRPILKNF PQVDHHQASG KLGDLYNDIH DTLRVPWVAF GIRVMSQFEH FVPAAWEALK
PQISTRYAEE GADKVREAAI IPGSAPANPT PALLANGWSE EEIAKLKATL DGLNYGNPKY
LILISAWNEA WHGRDAGGGA GKRLDSVQSE RLPYGLPQGV EKFHLIDPEA ADDQVQCLLR
DIRDAFLHHG PASDYRVLAA WPDYLEIAFR DTLKPVALTT EFELTTSRIR KIAREHVRGF
DGAGGVAWRD MADRMTPEEI AGLTGVLFMY NRFIADITVA IIRLKQAFGS AEDATENKFR
VWPTEKG


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