Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

AP2-associated protein kinase 1 (EC 2.7.11.1) (Adaptor-associated kinase 1)

 AAK1_PIG                Reviewed;         968 AA.
F1SPM8;
19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
19-OCT-2011, sequence version 2.
25-OCT-2017, entry version 37.
RecName: Full=AP2-associated protein kinase 1 {ECO:0000303|PubMed:11877457};
EC=2.7.11.1 {ECO:0000250|UniProtKB:P0C1X8};
AltName: Full=Adaptor-associated kinase 1 {ECO:0000303|PubMed:11877457};
Name=AAK1;
Sus scrofa (Pig).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
Sus.
NCBI_TaxID=9823;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Porcine genome sequencing project;
Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-54.
Uenishi H., Morozumi T., Toki D., Eguchi-Ogawa T., Tanaka-Matsuda M.;
"Porcine ESTs using full-length-enriched cDNA libraries.";
Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
[3] {ECO:0000305}
FUNCTION.
PubMed=11877457; DOI=10.1083/jcb.200111068;
Ricotta D., Conner S.D., Schmid S.L., von Figura K., Honing S.;
"Phosphorylation of the AP2 mu subunit by AAK1 mediates high affinity
binding to membrane protein sorting signals.";
J. Cell Biol. 156:791-795(2002).
-!- FUNCTION: Regulates clathrin-mediated endocytosis by
phosphorylating the AP2M1/mu2 subunit of the adaptor protein
complex 2 (AP-2) which ensures high affinity binding of AP-2 to
cargo membrane proteins during the initial stages of endocytosis.
Regulates phosphorylation of other AP-2 subunits as well as AP-2
localization and AP-2-mediated internalization of ligand
complexes. Phosphorylates NUMB and regulates its cellular
localization, promoting NUMB localization to endosomes. Binds to
and stabilizes the activated form of NOTCH1, increases its
localization in endosomes and regulates its transcriptional
activity. {ECO:0000269|PubMed:11877457}.
-!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
{ECO:0000250|UniProtKB:P0C1X8}.
-!- ENZYME REGULATION: Stimulated by clathrin.
{ECO:0000250|UniProtKB:P0C1X8}.
-!- SUBUNIT: Interacts with alpha-adaptin, AP-2, clathrin, NUMB and
EPS15. Interacts with membrane-bound activated NOTCH1 but not with
the inactive full-length form of NOTCH1. Preferentially interacts
with monoubiquitinated activated NOTCH1 compared to the non-
ubiquitinated form (By similarity). {ECO:0000250}.
-!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:F1MH24,
ECO:0000250|UniProtKB:P0C1X8}; Peripheral membrane protein
{ECO:0000250|UniProtKB:F1MH24, ECO:0000250|UniProtKB:P0C1X8}.
Membrane, clathrin-coated pit {ECO:0000250|UniProtKB:F1MH24,
ECO:0000250|UniProtKB:P0C1X8}. Note=Active when found in clathrin-
coated pits at the plasma membrane. In neuronal cells, enriched at
presynaptic terminals. In non-neuronal cells, enriched at leading
edge of migrating cells (By similarity).
{ECO:0000250|UniProtKB:F1MH24, ECO:0000250|UniProtKB:P0C1X8}.
-!- PTM: Autophosphorylated. {ECO:0000250|UniProtKB:P0C1X8}.
-!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr
protein kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; CU467585; -; NOT_ANNOTATED_CDS; Genomic_DNA.
EMBL; FS674854; -; NOT_ANNOTATED_CDS; mRNA.
SMR; F1SPM8; -.
STRING; 9823.ENSSSCP00000008900; -.
PaxDb; F1SPM8; -.
PeptideAtlas; F1SPM8; -.
PRIDE; F1SPM8; -.
eggNOG; KOG1989; Eukaryota.
eggNOG; ENOG410Y515; LUCA.
InParanoid; F1SPM8; -.
Proteomes; UP000008227; Unplaced.
GO; GO:0031252; C:cell leading edge; ISS:UniProtKB.
GO; GO:0005905; C:clathrin-coated pit; IEA:UniProtKB-SubCell.
GO; GO:0030136; C:clathrin-coated vesicle; IDA:UniProtKB.
GO; GO:0019897; C:extrinsic component of plasma membrane; ISS:UniProtKB.
GO; GO:0043195; C:terminal bouton; ISS:UniProtKB.
GO; GO:0035612; F:AP-2 adaptor complex binding; ISS:UniProtKB.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0005112; F:Notch binding; ISS:UniProtKB.
GO; GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
GO; GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
GO; GO:0045747; P:positive regulation of Notch signaling pathway; ISS:UniProtKB.
GO; GO:0046777; P:protein autophosphorylation; ISS:UniProtKB.
GO; GO:0006468; P:protein phosphorylation; IDA:UniProtKB.
GO; GO:0050821; P:protein stabilization; ISS:UniProtKB.
GO; GO:2000369; P:regulation of clathrin-dependent endocytosis; IDA:UniProtKB.
GO; GO:0032880; P:regulation of protein localization; ISS:UniProtKB.
Gene3D; 1.10.565.10; -; 1.
InterPro; IPR011009; Kinase-like_dom.
InterPro; IPR035500; NHR_like_domain.
InterPro; IPR000719; Prot_kinase_dom.
InterPro; IPR008271; Ser/Thr_kinase_AS.
Pfam; PF00069; Pkinase; 1.
SMART; SM00220; S_TKc; 1.
SUPFAM; SSF56112; SSF56112; 1.
PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
2: Evidence at transcript level;
Acetylation; ATP-binding; Cell membrane; Coated pit;
Complete proteome; Endocytosis; Kinase; Membrane; Methylation;
Nucleotide-binding; Phosphoprotein; Reference proteome;
Serine/threonine-protein kinase; Transferase.
CHAIN 1 968 AP2-associated protein kinase 1.
/FTId=PRO_0000413362.
DOMAIN 46 315 Protein kinase. {ECO:0000255|PROSITE-
ProRule:PRU00159}.
NP_BIND 52 60 ATP. {ECO:0000255|PROSITE-
ProRule:PRU00159}.
COMPBIAS 397 621 Gln-rich.
COMPBIAS 665 670 Poly-Ala.
ACT_SITE 176 176 Proton acceptor. {ECO:0000255|PROSITE-
ProRule:PRU00159, ECO:0000255|PROSITE-
ProRule:PRU10027}.
BINDING 74 74 ATP. {ECO:0000255|PROSITE-
ProRule:PRU00159}.
MOD_RES 1 1 N-acetylmethionine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 14 14 Phosphoserine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 234 234 Phosphotyrosine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 235 235 Phosphoserine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 354 354 Phosphothreonine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 389 389 Phosphothreonine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 391 391 Omega-N-methylarginine.
{ECO:0000250|UniProtKB:Q3UHJ0}.
MOD_RES 613 613 Phosphothreonine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 625 625 Phosphoserine.
{ECO:0000250|UniProtKB:P0C1X8}.
MOD_RES 627 627 Phosphothreonine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 630 630 Phosphoserine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 631 631 Phosphoserine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 644 644 Phosphoserine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 657 657 Phosphoserine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 660 660 Phosphothreonine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 694 694 Phosphotyrosine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 738 738 Phosphoserine.
{ECO:0000250|UniProtKB:Q2M2I8}.
MOD_RES 853 853 Phosphoserine.
{ECO:0000250|UniProtKB:Q3UHJ0}.
MOD_RES 944 944 Phosphoserine.
{ECO:0000250|UniProtKB:Q3UHJ0}.
MOD_RES 945 945 Phosphoserine.
{ECO:0000250|UniProtKB:Q3UHJ0}.
SEQUENCE 968 AA; 104310 MW; 4F07200E056E047D CRC64;
MKKFFDSRRE QGGSGLGSGS SGGGGSTSGL GSGYIGRVFG IGRQQVTVDE VLAEGGFAIV
FLVRTSNGMK CALKRMFVNN EHDLQVCKRE IQIMRDLSGH KNIVGYIDSS INNVSSGDVW
EVLILMDFCR GGQVVNLMNQ RLQTGFTESE VLQIFCDTCE AVARLHQCKT PIIHRDLKVE
NILLHDRGHY VLCDFGSATN KFQNPQTEGV NAVEDEIKKY TTLSYRAPEM VNLYSGKIIT
TKADIWALGC LLYKLCYFTL PFGESQVAIC DGNFTIPDNS RYSQDMHCLI RYMLEPDPDK
RPDIYQVSFF SFKLLKKECP VPNVQNSPIP AKLPEPVKAS EAAAKKTQPK ARLTDPIPTT
ETSIAPRQRP KAGQTQPNPG ILPIQPALTP RKRAMVQPPP QVAGSSNQPG LLASVPQPKT
QAPPSQPLPQ SQAKQPQAPP APQQPPSAPA QGLPAQAQAT PQHQQQLFLK QQPQPPQPQP
QAQAPPVKSL KFYPFYPMCK GRQTVSSQFQ AVHPAAQQPA IAQFPAVSQG SSQQQLIQNF
YQQQQQQQQQ QQLATTLHQQ QLLTQQAALQ QKTTVAAIQP PQAQPATASQ PPPAQEPAQI
QAPVRQQPKV QTTPPPAIQG QKLGSLTPPS SPKAQRAGHR RILSDVTHSA VFGVPASKST
QLLQAAAAEA SLNKSKSATT TPSGSPRASQ QNVYNPSEGS TWNPFDDDNF SKLTAEELLN
KDFAKLGEGK HPEKLGGSAE SLIPGFQPTQ GDAFAASSFA AGTAEKRKGG QAVDSSLPLL
SVSDPFIPLQ VPDAPEKLIE GLKSPDTSLL LPDLLPMTDP FGSTSDAVIE KADVAVESLI
PGLEPPVPQR LPSQTESVAS NRTDSLTGED ALIDCSLLSN PTTDLLEEFA PIAISAPAHK
AAEDSNLISG FDVPEGSDKV AEDEFDPIPV LITKNPQGGH SRNSSGSSES SLPNLARSLL
LVDQLIDL


Related products :

Catalog number Product name Quantity
EIAAB24783 GCK family kinase MiNK,Map4k6,MAPK_ERK kinase kinase kinase 6,MEK kinase kinase 6,MEKKK 6,Mink,Mink1,Misshapen_NIK-related kinase,Misshapen-like kinase 1,Mitogen-activated protein kinase kinase kinase
EIAAB24784 GCK family kinase MiNK,Map4k6,MAPK_ERK kinase kinase kinase 6,MEK kinase kinase 6,MEKKK 6,Mink,Mink1,Misshapen_NIK-related kinase,Misshapen-like kinase 1,Mitogen-activated protein kinase kinase kinase
EIAAB25239 C-JUN N-terminal kinase kinase 1,Dual specificity mitogen-activated protein kinase kinase 4,JNK kinase 1,JNK-activating kinase 1,JNKK 1,Jnkk1,MAP kinase kinase 4,Map2k4,MAPK_ERK kinase 4,MAPKK 4,MEK 4
EIAAB25244 c-Jun N-terminal kinase kinase 2,Dual specificity mitogen-activated protein kinase kinase 7,JNK kinase 2,JNK-activating kinase 2,JNKK 2,MAP kinase kinase 7,Map2k7,MAPK_ERK kinase 7,MAPKK 7,MEK 7,Mkk7,
EIAAB25245 c-Jun N-terminal kinase kinase 2,Dual specificity mitogen-activated protein kinase kinase 7,JNK kinase 2,JNK-activating kinase 2,JNKK 2,MAP kinase kinase 7,Map2k7,MAPK_ERK kinase 7,MAPKK 7,MEK 7,Rat,R
EIAAB25246 c-Jun N-terminal kinase kinase 2,Dual specificity mitogen-activated protein kinase kinase 7,Homo sapiens,Human,JNK kinase 2,JNK-activating kinase 2,JNKK 2,JNKK2,MAP kinase kinase 7,MAP2K7,MAPK_ERK kin
EIAAB25240 c-Jun N-terminal kinase kinase 1,Dual specificity mitogen-activated protein kinase kinase 4,Homo sapiens,Human,JNK-activating kinase 1,JNKK,JNKK1,MAP kinase kinase 4,MAP2K4,MAPK_ERK kinase 4,MAPKK 4,M
U1358m CLIA Apoptosis signal-regulating kinase 1,Ask1,ASK-1,Map3k5,MAPK_ERK kinase kinase 5,MEK kinase 5,MEKK 5,Mekk5,Mitogen-activated protein kinase kinase kinase 5,Mouse,Mus musculus 96T
E1358m ELISA Apoptosis signal-regulating kinase 1,Ask1,ASK-1,Map3k5,MAPK_ERK kinase kinase 5,MEK kinase 5,MEKK 5,Mekk5,Mitogen-activated protein kinase kinase kinase 5,Mouse,Mus musculus 96T
E1358m ELISA kit Apoptosis signal-regulating kinase 1,Ask1,ASK-1,Map3k5,MAPK_ERK kinase kinase 5,MEK kinase 5,MEKK 5,Mekk5,Mitogen-activated protein kinase kinase kinase 5,Mouse,Mus musculus 96T
EIAAB31333 Homo sapiens,Human,PAK1,PAK-1,PKN,PKN1,PRK1,PRKCL1,Protease-activated kinase 1,Protein kinase C-like 1,Protein kinase C-like PKN,Protein kinase PKN-alpha,Protein-kinase C-related kinase 1,Serine_threo
EIAAB31334 Bos taurus,Bovine,PKN,PKN1,PRK1,PRKCL1,Protein kinase C-like 1,Protein kinase C-like PKN,Protein kinase PKN-alpha,Protein-kinase C-related kinase 1,Serine_threonine-protein kinase N1,Serine-threonine
E1358h ELISA kit Apoptosis signal-regulating kinase 1,ASK1,ASK-1,Homo sapiens,Human,MAP3K5,MAPK_ERK kinase kinase 5,MAPKKK5,MEK kinase 5,MEKK 5,MEKK5,Mitogen-activated protein kinase kinase kinase 5 96T
U1358h CLIA Apoptosis signal-regulating kinase 1,ASK1,ASK-1,Homo sapiens,Human,MAP3K5,MAPK_ERK kinase kinase 5,MAPKKK5,MEK kinase 5,MEKK 5,MEKK5,Mitogen-activated protein kinase kinase kinase 5 96T
E1358h ELISA Apoptosis signal-regulating kinase 1,ASK1,ASK-1,Homo sapiens,Human,MAP3K5,MAPK_ERK kinase kinase 5,MAPKKK5,MEK kinase 5,MEKK 5,MEKK5,Mitogen-activated protein kinase kinase kinase 5 96T
EIAAB31331 PAK-1,Pkn,Pkn1,Prk1,Prkcl1,Protease-activated kinase 1,Protein kinase C-like 1,Protein kinase C-like PKN,Protein-kinase C-related kinase 1,Rat,Rattus norvegicus,Serine_threonine-protein kinase N1,Seri
EIAAB31332 Mouse,Mus musculus,Pkn,Pkn1,Prk1,Prkcl1,Protein kinase C-like 1,Protein kinase C-like PKN,Protein-kinase C-related kinase 1,Serine_threonine-protein kinase N1,Serine-threonine protein kinase N
EIAAB30987 Homo sapiens,Human,Phosphoinositide 3-kinase adaptor protein,Phosphoinositide 3-kinase regulatory subunit 4,PI3-kinase p150 subunit,PI3-kinase regulatory subunit 4,PIK3R4
18-662-20091 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 Polyclonal 0.1 ml
15-288-21068 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 Polyclonal 0.05 mg
15-288-21068 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 Polyclonal 0.1 mg
18-662-20092 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 Polyclonal 0.1 ml
18-662-20090 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 Polyclonal 0.1 ml
10-782-55048 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 N_A 0.05 mg
10-782-55047 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 N_A 0.02 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur