Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Acetoacetate decarboxylase (AAD) (ADC) (EC 4.1.1.4)

 ADC_CLOAB               Reviewed;         244 AA.
P23670; Q9S428;
01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
01-NOV-1991, sequence version 1.
22-NOV-2017, entry version 116.
RecName: Full=Acetoacetate decarboxylase;
Short=AAD;
Short=ADC;
EC=4.1.1.4;
Name=adc; OrderedLocusNames=CA_P0165;
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG
5710 / VKM B-1787).
Plasmid pSOL1.
Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
Clostridium.
NCBI_TaxID=272562;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-25.
STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
PubMed=2254264; DOI=10.1128/jb.172.12.6907-6918.1990;
Gerischer U., Duerre P.;
"Cloning, sequencing, and molecular analysis of the acetoacetate
decarboxylase gene region from Clostridium acetobutylicum.";
J. Bacteriol. 172:6907-6918(1990).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-30.
STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
PubMed=8423010; DOI=10.1016/0378-1119(93)90545-E;
Petersen D.J., Cary J.W., Vanderleyden J., Bennett G.N.;
"Sequence and arrangement of genes encoding enzymes of the acetone-
production pathway of Clostridium acetobutylicum ATCC824.";
Gene 123:93-97(1993).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
PubMed=11466286; DOI=10.1128/JB.183.16.4823-4838.2001;
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q.,
Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I.,
Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P.,
Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.;
"Genome sequence and comparative analysis of the solvent-producing
bacterium Clostridium acetobutylicum.";
J. Bacteriol. 183:4823-4838(2001).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 207-244.
STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
PubMed=8226639; DOI=10.1128/jb.175.21.6959-6969.1993;
Fischer R.J., Helms J., Duerre P.;
"Cloning, sequencing, and molecular analysis of the sol operon of
Clostridium acetobutylicum, a chromosomal locus involved in
solventogenesis.";
J. Bacteriol. 175:6959-6969(1993).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
Sabathe F., Cornillot E., Croux C., Soucaille P.;
"Molecular characterization of amyP, a pSOL1 located gene coding the
major alpha-amylase of Clostridium acetobutylicum ATCC824, and its use
as a reporter system for strain degeneration.";
Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
[6]
PROTEIN SEQUENCE OF 1-20.
STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
PubMed=2268159;
Petersen D.J., Bennett G.N.;
"Purification of acetoacetate decarboxylase from Clostridium
acetobutylicum ATCC 824 and cloning of the acetoacetate decarboxylase
gene in Escherichia coli.";
Appl. Environ. Microbiol. 56:3491-3498(1990).
[7]
ACTIVE SITE.
PubMed=5946601; DOI=10.1021/ja00966a044;
Laursen R.A., Westheimer F.H.;
"The active site of acetoacetate decarboxylase.";
J. Am. Chem. Soc. 88:3426-3430(1966).
[8]
CATALYTIC MECHANISM, AND MUTAGENESIS OF LYS-115 AND LYS-116.
PubMed=8555196; DOI=10.1021/bi9518306;
Highbarger L.A., Gerlt J.A., Kenyon G.L.;
"Mechanism of the reaction catalyzed by acetoacetate decarboxylase.
Importance of lysine 116 in determining the pKa of active-site lysine
115.";
Biochemistry 35:41-46(1996).
-!- FUNCTION: Catalyzes the conversion of acetoacetate to acetone and
carbon dioxide.
-!- CATALYTIC ACTIVITY: Acetoacetate = acetone + CO(2).
-!- BIOPHYSICOCHEMICAL PROPERTIES:
pH dependence:
Optimum pH is 5.95.;
-!- SUBUNIT: Homododecamer.
-!- INTERACTION:
Self; NbExp=4; IntAct=EBI-15782069, EBI-15782069;
-!- INDUCTION: By linear fatty acids (up to butyrate).
-!- SIMILARITY: Belongs to the ADC family. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; M55392; AAA63761.1; -; Genomic_DNA.
EMBL; M93363; AAB53232.1; -; Genomic_DNA.
EMBL; AE001438; AAK76910.1; -; Genomic_DNA.
EMBL; X72831; CAA51347.1; -; Genomic_DNA.
EMBL; AF164199; AAD47074.1; -; Genomic_DNA.
PIR; JN0487; JN0487.
RefSeq; NP_149328.1; NC_001988.2.
RefSeq; WP_010890849.1; NC_001988.2.
PDB; 3BH2; X-ray; 2.40 A; A/B/C/D=1-244.
PDBsum; 3BH2; -.
ProteinModelPortal; P23670; -.
SMR; P23670; -.
DIP; DIP-59756N; -.
EnsemblBacteria; AAK76910; AAK76910; CA_P0165.
GeneID; 1116170; -.
KEGG; cac:CA_P0165; -.
PATRIC; fig|272562.8.peg.166; -.
HOGENOM; HOG000063822; -.
KO; K01574; -.
OMA; CTPRICE; -.
BioCyc; MetaCyc:ADCCLOS-MONOMER; -.
BRENDA; 4.1.1.4; 1452.
EvolutionaryTrace; P23670; -.
Proteomes; UP000000814; Plasmid pSOL1.
GO; GO:0047602; F:acetoacetate decarboxylase activity; IEA:UniProtKB-EC.
GO; GO:0042802; F:identical protein binding; IPI:IntAct.
Gene3D; 2.40.400.10; -; 1.
HAMAP; MF_00597; ADC; 1.
InterPro; IPR010451; Acetoacetate_decarboxylase.
InterPro; IPR023653; Acetoacetate_decarboxylase_bac.
InterPro; IPR023375; ADC_dom_sf.
Pfam; PF06314; ADC; 1.
SUPFAM; SSF160104; SSF160104; 1.
1: Evidence at protein level;
3D-structure; Complete proteome; Decarboxylase;
Direct protein sequencing; Lyase; Plasmid; Reference proteome;
Schiff base.
CHAIN 1 244 Acetoacetate decarboxylase.
/FTId=PRO_0000207101.
ACT_SITE 115 115 Schiff-base intermediate with
acetoacetate.
{ECO:0000269|PubMed:5946601}.
MUTAGEN 115 115 K->C,Q: Complete loss of activity.
{ECO:0000269|PubMed:8555196}.
MUTAGEN 116 116 K->C,N,R: Small loss of activity.
{ECO:0000269|PubMed:8555196}.
CONFLICT 85 85 F -> C (in Ref. 2; AAB53232).
{ECO:0000305}.
HELIX 3 7 {ECO:0000244|PDB:3BH2}.
STRAND 11 13 {ECO:0000244|PDB:3BH2}.
STRAND 25 37 {ECO:0000244|PDB:3BH2}.
HELIX 40 46 {ECO:0000244|PDB:3BH2}.
STRAND 57 68 {ECO:0000244|PDB:3BH2}.
TURN 69 71 {ECO:0000244|PDB:3BH2}.
STRAND 72 85 {ECO:0000244|PDB:3BH2}.
STRAND 88 99 {ECO:0000244|PDB:3BH2}.
HELIX 101 109 {ECO:0000244|PDB:3BH2}.
STRAND 115 117 {ECO:0000244|PDB:3BH2}.
STRAND 119 125 {ECO:0000244|PDB:3BH2}.
STRAND 128 135 {ECO:0000244|PDB:3BH2}.
STRAND 138 146 {ECO:0000244|PDB:3BH2}.
HELIX 154 161 {ECO:0000244|PDB:3BH2}.
STRAND 165 172 {ECO:0000244|PDB:3BH2}.
STRAND 176 186 {ECO:0000244|PDB:3BH2}.
STRAND 190 198 {ECO:0000244|PDB:3BH2}.
STRAND 201 205 {ECO:0000244|PDB:3BH2}.
HELIX 213 215 {ECO:0000244|PDB:3BH2}.
STRAND 219 232 {ECO:0000244|PDB:3BH2}.
STRAND 236 241 {ECO:0000244|PDB:3BH2}.
SEQUENCE 244 AA; 27537 MW; CA6C1157644E13B0 CRC64;
MLKDEVIKQI STPLTSPAFP RGPYKFHNRE YFNIVYRTDM DALRKVVPEP LEIDEPLVRF
EIMAMHDTSG LGCYTESGQA IPVSFNGVKG DYLHMMYLDN EPAIAVGREL SAYPKKLGYP
KLFVDSDTLV GTLDYGKLRV ATATMGYKHK ALDANEAKDQ ICRPNYMLKI IPNYDGSPRI
CELINAKITD VTVHEAWTGP TRLQLFDHAM APLNDLPVKE IVSSSHILAD IILPRAEVIY
DYLK


Related products :

Catalog number Product name Quantity
15933-07-0 Ethyl acetoacetate, sodium salt Ethyl acetoacetate, sod 1g
34284-28-1 methyl acetoacetate, monosodium salt methyl acetoacetate, m 1g
E1258h ELISA kit 67 kDa glutamic acid decarboxylase,GAD,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Homo sapiens,Human 96T
E1258h ELISA 67 kDa glutamic acid decarboxylase,GAD,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Homo sapiens,Human 96T
U1258h CLIA 67 kDa glutamic acid decarboxylase,GAD,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Homo sapiens,Human 96T
EIAAB09546 CSAD,CSD,Cysteine sulfinic acid decarboxylase,Cysteine-sulfinate decarboxylase,Homo sapiens,Human,Sulfinoalanine decarboxylase
U1258m CLIA 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Mouse,Mus musculus 96T
E1258m ELISA kit 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Mouse,Mus musculus 96T
E1258m ELISA 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Mouse,Mus musculus 96T
U1258r CLIA 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Rat,Rattus norvegicus 96T
E1258r ELISA 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Rat,Rattus norvegicus 96T
EIAAB10595 65 kDa glutamic acid decarboxylase,GAD2,GAD65,GAD-65,Glutamate decarboxylase 2,Glutamate decarboxylase 65 kDa isoform,Homo sapiens,Human
E1258r ELISA kit 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Rat,Rattus norvegicus 96T
EIAAB09547 Csad,Cysteine sulfinic acid decarboxylase,Cysteine-sulfinate decarboxylase,Mouse,Mus musculus,Sulfinoalanine decarboxylase
EIAAB25871 Diphosphomevalonate decarboxylase,Homo sapiens,Human,MDDase,Mevalonate (diphospho)decarboxylase,Mevalonate pyrophosphate decarboxylase,MPD,MVD
EIAAB10592 65 kDa glutamic acid decarboxylase,Gad2,Gad65,GAD-65,Glutamate decarboxylase 2,Glutamate decarboxylase 65 kDa isoform,Rat,Rattus norvegicus
E1258p ELISA kit 67 kDa glutamic acid decarboxylase,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Pig,Sus scrofa 96T
EIAAB10594 65 kDa glutamic acid decarboxylase,Gad2,Gad65,GAD-65,Glutamate decarboxylase 2,Glutamate decarboxylase 65 kDa isoform,Mouse,Mus musculus
EIAAB09548 Csad,Csd,Cysteine sulfinic acid decarboxylase,Cysteine-sulfinate decarboxylase,Rat,Rattus norvegicus,Sulfinoalanine decarboxylase
18-783-77682 RABBIT ANTI HUMAN GAD 65 - GLUTAMATE DECARBOXYLASE; EC 4.1.1.15; Glutamate decarboxylase 67 kDa isoform; GAD-67; 67 kDa glutamic acid decarboxylase Polyclonal 1 ml
E1258p ELISA 67 kDa glutamic acid decarboxylase,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Pig,Sus scrofa 96T
U1258p CLIA 67 kDa glutamic acid decarboxylase,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Pig,Sus scrofa 96T
EIAAB25870 Bos taurus,Bovine,Diphosphomevalonate decarboxylase,MDDase,Mevalonate (diphospho)decarboxylase,Mevalonate pyrophosphate decarboxylase,MVD
EIAAB25869 Diphosphomevalonate decarboxylase,MDDase,Mevalonate (diphospho)decarboxylase,Mevalonate pyrophosphate decarboxylase,Mouse,Mus musculus,Mvd
EIAAB10591 65 kDa glutamic acid decarboxylase,GAD2,GAD65,GAD-65,Glutamate decarboxylase 2,Glutamate decarboxylase 65 kDa isoform,Pig,Sus scrofa


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur