Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Acyl-[acyl-carrier-protein] 6-desaturase (EC 1.14.19.26) (Palmitoyl-acyl carrier protein desaturase)

 PALAD_THUAT             Reviewed;         387 AA.
Q41510;
11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
28-FEB-2018, entry version 77.
RecName: Full=Acyl-[acyl-carrier-protein] 6-desaturase {ECO:0000305};
EC=1.14.19.26 {ECO:0000269|PubMed:7961667, ECO:0000269|PubMed:9144157};
AltName: Full=Palmitoyl-acyl carrier protein desaturase {ECO:0000303|PubMed:7961667};
Flags: Precursor;
Thunbergia alata (Black-eyed Susan vine).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; asterids; lamiids; Lamiales; Acanthaceae;
Thunbergioideae; Thunbergia.
NCBI_TaxID=32198 {ECO:0000312|EMBL:AAA82160.1};
[1]
NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION, SUBSTRATE
SPECIFICITY, AND ENZYME REGULATION.
TISSUE=Endosperm;
PubMed=7961667;
Cahoon E.B., Cranmer A.M., Shanklin J., Ohlrogge J.B.;
"delta 6 Hexadecenoic acid is synthesized by the activity of a soluble
delta 6 palmitoyl-acyl carrier protein desaturase in Thunbergia alata
endosperm.";
J. Biol. Chem. 269:27519-27526(1994).
[2]
3D-STRUCTURE MODELING, MUTAGENESIS OF ALA-207; ALA-214; TYR-215;
ALA-226; SER-231; LEU-232 AND GLY-233, AND CATALYTIC ACTIVITY.
PubMed=9144157; DOI=10.1073/pnas.94.10.4872;
Cahoon E.B., Lindqvist Y., Schneider G., Shanklin J.;
"Redesign of soluble fatty acid desaturases from plants for altered
substrate specificity and double bond position.";
Proc. Natl. Acad. Sci. U.S.A. 94:4872-4877(1997).
-!- FUNCTION: Delta(6) fatty acid desaturase introducing a cis double
bond at carbon 6 of palmitoyl-[acyl-carrier protein](16:0-ACP),
producing 16:1(6Z)-ACP (PubMed:7961667, PubMed:9144157). No
activity with the coenzyme A ester of the fatty acid
(PubMed:7961667). The position of the double bond is determined by
its distance from the carboxyl end of the fatty acid
(PubMed:7961667). Low activity with several saturated acyl-[acyl-
carrier protein]s, including 14:0-ACP and 18:0-ACP
(PubMed:7961667, PubMed:9144157). Requires reduced ferredoxin for
detectable in vitro activity (PubMed:7961667).
{ECO:0000269|PubMed:7961667, ECO:0000269|PubMed:9144157}.
-!- CATALYTIC ACTIVITY: Palmitoyl-[acyl-carrier protein] + 2 reduced
ferredoxin [iron-sulfur] cluster + O(2) + 2 H(+) = (6Z)-hexadec-6-
enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur]
cluster + 2 H(2)O. {ECO:0000269|PubMed:7961667,
ECO:0000269|PubMed:9144157}.
-!- COFACTOR:
Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
Evidence={ECO:0000250|UniProtKB:P22337};
Note=Binds 2 Fe(2+) ions per subunit.
{ECO:0000250|UniProtKB:P22337};
-!- ENZYME REGULATION: Inhibited by KCN or H(2)O(2).
{ECO:0000269|PubMed:7961667}.
-!- PATHWAY: Lipid metabolism; fatty acid metabolism.
-!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
-!- SIMILARITY: Belongs to the fatty acid desaturase type 2 family.
{ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; U09269; AAA82160.1; -; mRNA.
ProteinModelPortal; Q41510; -.
SMR; Q41510; -.
BioCyc; MetaCyc:MONOMER-12584; -.
UniPathway; UPA00199; -.
GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
GO; GO:0045300; F:acyl-[acyl-carrier-protein] desaturase activity; IEA:InterPro.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
CDD; cd01050; Acyl_ACP_Desat; 1.
Gene3D; 1.10.620.20; -; 1.
InterPro; IPR005067; Fatty_acid_desaturase-2.
InterPro; IPR009078; Ferritin-like_SF.
InterPro; IPR012348; RNR-like.
PANTHER; PTHR31155; PTHR31155; 1.
Pfam; PF03405; FA_desaturase_2; 1.
PIRSF; PIRSF000346; Dlt9_acylACP_des; 1.
SUPFAM; SSF47240; SSF47240; 1.
1: Evidence at protein level;
Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism; Iron;
Lipid biosynthesis; Lipid metabolism; Metal-binding; Oxidoreductase;
Plastid; Transit peptide.
TRANSIT 1 29 Chloroplast. {ECO:0000255}.
CHAIN 30 387 Acyl-[acyl-carrier-protein] 6-desaturase.
/FTId=PRO_0000434767.
METAL 131 131 Iron 1. {ECO:0000250|UniProtKB:P22337}.
METAL 169 169 Iron 1. {ECO:0000250|UniProtKB:P22337}.
METAL 169 169 Iron 2. {ECO:0000250|UniProtKB:P22337}.
METAL 172 172 Iron 1; via pros nitrogen.
{ECO:0000250|UniProtKB:P22337}.
METAL 222 222 Iron 2. {ECO:0000250|UniProtKB:P22337}.
METAL 255 255 Iron 1. {ECO:0000250|UniProtKB:P22337}.
METAL 255 255 Iron 2. {ECO:0000250|UniProtKB:P22337}.
METAL 258 258 Iron 2; via pros nitrogen.
{ECO:0000250|UniProtKB:P22337}.
MUTAGEN 207 207 A->T: Catalytic activity converted to a
delta(9)-18:0-ACP desaturase; when
associated with F-226; N-231; T-232 and
A-233. Catalyzes both delta(9)
desaturation of 18:0-ACP and delta(6)
desaturation of 16:0-ACP; when associated
with F-226. Gain of a delta(9) desaturase
activity with both 18:0-ACP and 16:0-ACP;
when associated with F-226; N-231; T-232
and A-233. {ECO:0000269|PubMed:9144157}.
MUTAGEN 214 214 A->G: Broadened fatty acid chain-length
specificity; when associated with F-215.
{ECO:0000269|PubMed:9144157}.
MUTAGEN 215 215 Y->F: Broadened fatty acid chain-length
specificity; when associated with G-214.
{ECO:0000269|PubMed:9144157}.
MUTAGEN 226 226 A->F: Catalytic activity converted to a
delta(9)-18:0-ACP desaturase; when
associated with T-207; N-231; T-232 and
A-233. Catalyzes both delta(9)
desaturation of 18:0-ACP and delta(6)
desaturation of 16:0-ACP; when associated
with T-207. Gain of a delta(9) desaturase
activity with both 18:0-ACP and 16:0-ACP;
when associated with T-207; N-231; T-232
and A-233. {ECO:0000269|PubMed:9144157}.
MUTAGEN 231 231 S->N: Catalytic activity converted to a
delta(9)-18:0-ACP desaturase; when
associated with T-207; F-226; T-232 and
A-233. Gain of a delta(9) desaturase
activity with both 18:0-ACP and 16:0-ACP;
when associated with T-207; F-226; T-232
and A-233. {ECO:0000269|PubMed:9144157}.
MUTAGEN 232 232 L->T: Catalytic activity converted to a
delta(9)-18:0-ACP desaturase; when
associated with T-207; F-226; N-231 and
A-233. Gain of a delta(9) desaturase
activity with both 18:0-ACP and 16:0-ACP;
when associated with T-207; F-226; N-231
and A-233. {ECO:0000269|PubMed:9144157}.
MUTAGEN 233 233 G->A: Catalytic activity converted to a
delta(9)-18:0-ACP desaturase; when
associated with T-207; F-226; N-231 and
T-232. Gain of a delta(9) desaturase
activity with both 18:0-ACP and 16:0-ACP;
when associated with T-207; F-226; N-231
and T-232. {ECO:0000269|PubMed:9144157}.
SEQUENCE 387 AA; 43868 MW; B29E03180FB7EC68 CRC64;
MALVFKSIGA HKTPPCTLNL ASPALYHTRV TMASTITHPP PLKDRKISST RRVRTYPLAP
EKAEIFNSMH GWVEDTILPF LKPVEESWQP TDFLPDSTSD GFHEQVEELR KRTADLPDDY
LVALVGAMVT EEALPTYQTM LNTTDVIYDE SGASPVPWAV WTRAWTAEEN RHGDIVNKYL
YLSGRVDMKQ IEKTIQYLIG SGMDPGADNN PYLAYIYTSY QERATAISHG SLGRLARQKG
EMKLAQICGT ISADEKRHEA AYSKIVEKLF ELDPEGTMLA LAYMMKMKIV MPARLMHDGK
DPDMFQHFSA VSQRLGIYTA KEYTDILEHM IARWGVDKLT GLSGEGRRAQ DYVCGLPMRF
RKVEERAQAW AENISHVPFS WIFGRRV


Related products :

Catalog number Product name Quantity
18-003-42290 Acyl-CoA desaturase - EC 1.14.19.1; Stearoyl-CoA desaturase; Fatty acid desaturase; Delta(9)-desaturase Polyclonal 0.1 mg Protein A
EIAAB37528 ACOD4,Acyl-CoA-desaturase 4,Homo sapiens,HSCD5,Human,SCD4,SCD5,Stearoyl-CoA 9-desaturase,Stearoyl-CoA desaturase 5
EIAAB14290 [Acyl-carrier-protein] malonyltransferase,Homo sapiens,Human,Malonyl-CoA-acyl carrier protein transacylase, mitochondrial,MCAT,MCT,Mitochondrial malonyltransferase,MT
EIAAB14289 [Acyl-carrier-protein] malonyltransferase,Malonyl-CoA-acyl carrier protein transacylase, mitochondrial,Mcat,MCT,Mitochondrial malonyltransferase,Mouse,Mt,Mus musculus
EIAAB37529 Acyl-CoA-desaturase 4,Bos taurus,Bovine,SCD5,Stearoyl-CoA 9-desaturase,Stearoyl-CoA desaturase 5
orb70387 Acyl Carrier Protein (65-74) peptide This is Acyl Carrier Protein (65-74) peptide. For research use only. 1 mg
orb70386 Acyl Carrier Protein (65-74) peptide This is Acyl Carrier Protein (65-74) peptide. For research use only. 1 mg
25-764 FADS1 is a member of the fatty acid desaturase (FADS) family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl 0.05 mg
29-745 FADS1 is a member of the fatty acid desaturase (FADS) family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl 0.1 mg
CSB-EL020802PI Pig Acyl-CoA desaturase(SCD) ELISA kit SpeciesPig 96T
CSB-EL020802HU Human Acyl-CoA desaturase(SCD) ELISA kit 96T
CSB-EL020802BO Bovine Acyl-CoA desaturase(SCD) ELISA kit 96T
CSB-EL020802GO Goat Acyl-CoA desaturase(SCD) ELISA kit 96T
ARP32797_T200 Anti-Acyl-CoA Desaturase (SCD) Species_Reactivity: Human
CSB-EL020802BO Bovine Acyl-CoA desaturase(SCD) ELISA kit SpeciesBovine 96T
CSB-EL020802HU Human Acyl-CoA desaturase(SCD) ELISA kit SpeciesHuman 96T
CSB-EL020802GO Goat Acyl-CoA desaturase(SCD) ELISA kit SpeciesGoat 96T
CSB-EL020802SH Sheep Acyl-CoA desaturase(SCD) ELISA kit SpeciesSheep 96T
ACOD_BOVIN ELISA Kit FOR Acyl-CoA desaturase; organism: Bovine; gene name: SCD 96T
AS13 2668 rabbit polyclonal Plastid acyl-ACP desaturase 50
SP-86553-5 Acyl Carrier Protein (65-74) (acid) [Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly (MW: 1063.18)] 5 mg
SP-86554-5 Acyl Carrier Protein (65-74) (amide) (AA: Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-NH2) (MW: 1062.20) 5 mg
SP-86553-5 Acyl Carrier Protein (65_74) (acid) 5 mg
E1408m Human ELISA Kit FOR Acyl carrier protein, mitochondrial 96T
E2890h Human Malonyl CoA Acyl Carrier Protein Transacylas 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur