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Alpha-humulene/(-)-(E)-beta-caryophyllene synthase (EC 4.2.3.-) (Terpenoid synthase 21) (AtTPS21)

 HUMS_ARATH              Reviewed;         547 AA.
Q84UU4; Q84YI3; Q9FLW5;
02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
02-SEP-2008, sequence version 2.
20-DEC-2017, entry version 96.
RecName: Full=Alpha-humulene/(-)-(E)-beta-caryophyllene synthase;
EC=4.2.3.-;
AltName: Full=Terpenoid synthase 21;
Short=AtTPS21;
Name=TPS21; Synonyms=PUP8; OrderedLocusNames=At5g23960;
ORFNames=MZF18.16;
Arabidopsis thaliana (Mouse-ear cress).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
Arabidopsis.
NCBI_TaxID=3702;
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND TISSUE
SPECIFICITY.
STRAIN=cv. Landsberg erecta;
PubMed=12566586; DOI=10.1105/tpc.007989;
Chen F., Tholl D., D'Auria J.C., Farooq A., Pichersky E.,
Gershenzon J.;
"Biosynthesis and emission of terpenoid volatiles from Arabidopsis
flowers.";
Plant Cell 15:481-494(2003).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND TISSUE SPECIFICITY.
STRAIN=cv. Landsberg erecta; TISSUE=Flower;
PubMed=12805595; DOI=10.1104/pp.102.017798;
Scutt C.P., Vinauger-Douard M., Fourquin C., Ailhas J., Kuno N.,
Uchida K., Gaude T., Furuya M., Dumas C.;
"The identification of candidate genes for a reverse genetic analysis
of development and function in the Arabidopsis gynoecium.";
Plant Physiol. 132:653-665(2003).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
PubMed=9628582; DOI=10.1093/dnares/5.1.41;
Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. IV.
Sequence features of the regions of 1,456,315 bp covered by nineteen
physically assigned P1 and TAC clones.";
DNA Res. 5:41-54(1998).
[4]
GENOME REANNOTATION.
STRAIN=cv. Columbia;
The Arabidopsis Information Portal (Araport);
Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
[5]
GENE FAMILY, AND NOMENCLATURE.
PubMed=12207221; DOI=10.1007/s00438-002-0709-y;
Aubourg S., Lecharny A., Bohlmann J.;
"Genomic analysis of the terpenoid synthase (AtTPS) gene family of
Arabidopsis thaliana.";
Mol. Genet. Genomics 267:730-745(2002).
[6]
GENE FAMILY.
PubMed=12777052; DOI=10.1023/A:1023005504702;
Lange B.M., Ghassemian M.;
"Genome organization in Arabidopsis thaliana: a survey for genes
involved in isoprenoid and chlorophyll metabolism.";
Plant Mol. Biol. 51:925-948(2003).
[7]
FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND
BIOPHYSICOCHEMICAL PROPERTIES.
PubMed=15918888; DOI=10.1111/j.1365-313X.2005.02417.x;
Tholl D., Chen F., Petri J., Gershenzon J., Pichersky E.;
"Two sesquiterpene synthases are responsible for the complex mixture
of sesquiterpenes emitted from Arabidopsis flowers.";
Plant J. 42:757-771(2005).
[8]
INDUCTION BY HERBIVORY.
PubMed=18400103; DOI=10.1186/1471-2164-9-154;
Ehlting J., Chowrira S.G., Mattheus N., Aeschliman D.S., Arimura G.,
Bohlmann J.;
"Comparative transcriptome analysis of Arabidopsis thaliana infested
by diamond back moth (Plutella xylostella) larvae reveals signatures
of stress response, secondary metabolism, and signalling.";
BMC Genomics 9:154-154(2008).
-!- FUNCTION: Involved in sesquiterpene (C15) biosynthesis. The major
products are beta-caryophyllene and alpha-humulene. Does not
convert geranyl diphosphate (GPP) to any monoterpenes.
{ECO:0000269|PubMed:12566586, ECO:0000269|PubMed:15918888}.
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=2.1 uM for farnesyl diphosphate
{ECO:0000269|PubMed:15918888};
-!- PATHWAY: Secondary metabolite biosynthesis; terpenoid
biosynthesis.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
-!- ALTERNATIVE PRODUCTS:
Event=Alternative splicing; Named isoforms=2;
Name=1;
IsoId=Q84UU4-1; Sequence=Displayed;
Name=2;
IsoId=Q84UU4-2; Sequence=VSP_041593;
-!- TISSUE SPECIFICITY: Expressed exclusively in flowers. Expressed in
the flower stigmata and also detected in the mesocarp cell layers
of the silique wall. {ECO:0000269|PubMed:12566586,
ECO:0000269|PubMed:12805595, ECO:0000269|PubMed:15918888}.
-!- INDUCTION: Induced in response to the caterpillar P.xylostella
feeding. {ECO:0000269|PubMed:18400103}.
-!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important
for the catalytic activity, presumably through binding to Mg(2+).
-!- DISRUPTION PHENOTYPE: Does not emit (E)-beta-caryophyllene, alpha-
copaene and alpha-humulene. {ECO:0000269|PubMed:15918888}.
-!- SIMILARITY: Belongs to the terpene synthase family. Tpsa
subfamily. {ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=BAB08719.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
-----------------------------------------------------------------------
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-----------------------------------------------------------------------
EMBL; AF497491; AAO85539.1; -; mRNA.
EMBL; AJ544238; CAD66638.1; -; mRNA.
EMBL; AB009056; BAB08719.1; ALT_SEQ; Genomic_DNA.
EMBL; CP002688; AED93238.1; -; Genomic_DNA.
EMBL; CP002688; AED93239.1; -; Genomic_DNA.
RefSeq; NP_001190374.1; NM_001203445.1. [Q84UU4-2]
RefSeq; NP_197784.2; NM_122301.4. [Q84UU4-1]
UniGene; At.30985; -.
ProteinModelPortal; Q84UU4; -.
SMR; Q84UU4; -.
STRING; 3702.AT5G23960.1; -.
PaxDb; Q84UU4; -.
PRIDE; Q84UU4; -.
EnsemblPlants; AT5G23960.1; AT5G23960.1; AT5G23960. [Q84UU4-1]
EnsemblPlants; AT5G23960.2; AT5G23960.2; AT5G23960. [Q84UU4-2]
GeneID; 832461; -.
Gramene; AT5G23960.1; AT5G23960.1; AT5G23960. [Q84UU4-1]
Gramene; AT5G23960.2; AT5G23960.2; AT5G23960. [Q84UU4-2]
KEGG; ath:AT5G23960; -.
Araport; AT5G23960; -.
TAIR; locus:2178657; AT5G23960.
eggNOG; ENOG410IVC5; Eukaryota.
eggNOG; ENOG410YBXH; LUCA.
HOGENOM; HOG000232971; -.
InParanoid; Q84UU4; -.
KO; K14184; -.
OMA; IETRQYI; -.
OrthoDB; EOG0936092H; -.
PhylomeDB; Q84UU4; -.
BioCyc; ARA:AT5G23960-MONOMER; -.
BioCyc; MetaCyc:AT5G23960-MONOMER; -.
SABIO-RK; Q84UU4; -.
UniPathway; UPA00213; -.
PRO; PR:Q84UU4; -.
Proteomes; UP000006548; Chromosome 5.
ExpressionAtlas; Q84UU4; baseline and differential.
Genevisible; Q84UU4; AT.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0080016; F:(-)-E-beta-caryophyllene synthase activity; IDA:TAIR.
GO; GO:0080017; F:alpha-humulene synthase activity; IDA:TAIR.
GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
GO; GO:0080027; P:response to herbivore; IEP:UniProtKB.
GO; GO:0051762; P:sesquiterpene biosynthetic process; IDA:TAIR.
GO; GO:0016106; P:sesquiterpenoid biosynthetic process; IDA:TAIR.
Gene3D; 1.10.600.10; -; 1.
Gene3D; 1.50.10.130; -; 1.
InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
InterPro; IPR034741; Terpene_cyclase_like_1_C.
InterPro; IPR001906; Terpene_synth_N.
InterPro; IPR036965; Terpene_synth_N_sf.
InterPro; IPR005630; Terpene_synthase_metal-bd.
InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
Pfam; PF01397; Terpene_synth; 1.
Pfam; PF03936; Terpene_synth_C; 1.
SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
SUPFAM; SSF48239; SSF48239; 1.
SUPFAM; SSF48576; SSF48576; 1.
1: Evidence at protein level;
Alternative splicing; Complete proteome; Cytoplasm; Lyase; Magnesium;
Manganese; Metal-binding; Reference proteome.
CHAIN 1 547 Alpha-humulene/(-)-(E)-beta-caryophyllene
synthase.
/FTId=PRO_0000348421.
MOTIF 299 303 DDXXD motif.
COMPBIAS 214 217 Poly-Glu.
METAL 299 299 Magnesium or manganese 1. {ECO:0000250}.
METAL 299 299 Magnesium or manganese 2. {ECO:0000250}.
METAL 303 303 Magnesium or manganese 1. {ECO:0000250}.
METAL 303 303 Magnesium or manganese 2. {ECO:0000250}.
METAL 445 445 Magnesium or manganese 3. {ECO:0000250}.
METAL 449 449 Magnesium or manganese 3. {ECO:0000250}.
METAL 453 453 Magnesium or manganese 3. {ECO:0000250}.
VAR_SEQ 321 328 WLPVVPDE -> YSICNI (in isoform 2).
{ECO:0000303|PubMed:12805595}.
/FTId=VSP_041593.
CONFLICT 73 73 Y -> C (in Ref. 1; AAO85539).
{ECO:0000305}.
CONFLICT 406 406 A -> G (in Ref. 1; AAO85539).
{ECO:0000305}.
CONFLICT 428 428 S -> N (in Ref. 1; AAO85539).
{ECO:0000305}.
SEQUENCE 547 AA; 63619 MW; D9380654373B735D CRC64;
MGSEVNRPLA DFPANIWEDP LTSFSKSDLG TETFKEKHST LKEAVKEAFM SSKANPIENI
KFIDALCRLG VSYHFEKDIV EQLDKSFDCL DFPQMVRQEG CDLYTVGIIF QVFRQFGFKL
SADVFEKFKD ENGKFKGHLV TDAYGMLSLY EAAQWGTHGE DIIDEALAFS RSHLEEISSR
SSPHLAIRIK NALKHPYHKG ISRIETRQYI SYYEEEESCD PTLLEFAKID FNLLQILHRE
ELACVTRWHH EMEFKSKVTY TRHRITEAYL WSLGTYFEPQ YSQARVITTM ALILFTALDD
MYDAYGTMEE LELFTDAMDE WLPVVPDEIP IPDSMKFIYN VTVEFYDKLD EELEKEGRSG
CGFHLKKSLQ KTANGYMQEA KWLKKDYIAT FDEYKENAIL SSGYYALIAM TFVRMTDVAK
LDAFEWLSSH PKIRVASEII SRFTDDISSY EFEHKREHVA TGIDCYMQQF GVSKERAVEV
MGNIVSDAWK DLNQELMRPH VFPFPLLMRV LNLSRVIDVF YRYQDAYTNP KLLKEHIVSL
LIETIPI


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