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Alpha-mannosidase (JBM) (Jbalpha-man) (EC 3.2.1.24) [Cleaved into: Alpha-mannosidase, heavy subunit; Alpha-mannosidase, light subunit]

 MANA_CANEN              Reviewed;         981 AA.
C0HJB3;
26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
26-NOV-2014, sequence version 1.
25-APR-2018, entry version 12.
RecName: Full=Alpha-mannosidase {ECO:0000303|PubMed:4973951};
Short=JBM {ECO:0000303|PubMed:24295789};
Short=Jbalpha-man {ECO:0000303|PubMed:21924285};
EC=3.2.1.24 {ECO:0000269|PubMed:1156387, ECO:0000269|PubMed:12325362, ECO:0000269|PubMed:4973951, ECO:0000269|PubMed:5145};
Contains:
RecName: Full=Alpha-mannosidase, heavy subunit {ECO:0000303|PubMed:24221485};
Contains:
RecName: Full=Alpha-mannosidase, light subunit {ECO:0000303|PubMed:24221485};
Canavalia ensiformis (Jack bean) (Dolichos ensiformis).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae;
Phaseoleae; Canavalia.
NCBI_TaxID=3823 {ECO:0000303|PubMed:9442045};
[1]
PROTEIN SEQUENCE, PROTEOLYTIC PROCESSING, GLYCOSYLATION AT ASN-312 AND
ASN-446, DISULFIDE BONDS, IDENTIFICATION BY MASS SPECTROMETRY, AND
IDENTIFICATION OF CLEAVAGE SITE.
TISSUE=Seed {ECO:0000303|PubMed:24295789};
PubMed=24295789; DOI=10.1093/glycob/cwt106;
Gnanesh Kumar B.S., Pohlentz G., Schulte M., Mormann M.,
Siva Kumar N.;
"Jack bean alpha-mannosidase: amino acid sequencing and N-
glycosylation analysis of a valuable glycomics tool.";
Glycobiology 24:252-261(2014).
[2]
PROTEIN SEQUENCE OF 135-154, ENZYME REGULATION, MASS SPECTROMETRY,
ACTIVE SITE, AND PROTEOLYTIC PROCESSING.
PubMed=9442045; DOI=10.1074/jbc.273.4.2067;
Howard S., He S., Withers S.G.;
"Identification of the active site nucleophile in jack bean alpha-
mannosidase using 5-fluoro-beta-L-gulosyl fluoride.";
J. Biol. Chem. 273:2067-2072(1998).
[3] {ECO:0000305}
FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, AND
BIOPHYSICOCHEMICAL PROPERTIES.
PubMed=12325362;
Li Y.T.;
"Studies on the glycosidases in jack bean meal. I. Isolation and
properties of alpha-mannosidase.";
J. Biol. Chem. 242:5474-5480(1967).
[4] {ECO:0000305}
FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, COFACTOR, AND
BIOPHYSICOCHEMICAL PROPERTIES.
PubMed=4973951; DOI=10.1042/bj1100663;
Snaith S.M., Levvy G.A.;
"Purification and properties of alpha-D-mannosidase from jack-bean
meal.";
Biochem. J. 110:663-670(1968).
[5] {ECO:0000305}
FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, AND COFACTOR.
PubMed=1156387; DOI=10.1042/bj1470083;
Snaith S.M.;
"Characterization of jack-bean alpha-D-mannosidase as a zinc
metalloenzyme.";
Biochem. J. 147:83-90(1975).
[6] {ECO:0000305}
FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND
SUBUNIT.
PubMed=5145; DOI=10.1016/0005-2744(76)90334-X;
Sheperd V., Montgomery R.;
"Alpha-D-Mannosidase. Preparation and properties of free and
insolubilized enzyme.";
Biochim. Biophys. Acta 429:884-894(1976).
[7] {ECO:0000305}
PROTEOLYTIC CLEAVAGE, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
PubMed=24221485; DOI=10.1007/BF00394781;
Faye L., Greenwood J.S., Herman E.M., Sturm A., Chrispeels M.J.;
"Transport and posttranslational processing of the vacuolar enzyme
alpha-mannosidase in jack-bean cotyledons.";
Planta 174:271-282(1988).
[8] {ECO:0000305}
ENZYME REGULATION.
PubMed=15974577; DOI=10.1021/jm0409019;
Fiaux H., Popowycz F., Favre S., Schuetz C., Vogel P.,
Gerber-Lemaire S., Juillerat-Jeanneret L.;
"Functionalized pyrrolidines inhibit alpha-mannosidase activity and
growth of human glioblastoma and melanoma cells.";
J. Med. Chem. 48:4237-4246(2005).
[9] {ECO:0000305}
COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT,
AND PRESENCE OF DISULFIDE BONDS.
PubMed=21924285; DOI=10.1016/j.ijbiomac.2011.08.033;
Kumar A., Gaikwad S.M.;
"Jack bean alpha-mannosidase (Jbalpha-man): tolerance to alkali,
chelating and reducing agents and energetics of catalysis and
inhibition.";
Int. J. Biol. Macromol. 49:1066-1071(2011).
-!- FUNCTION: Liberates mannose from p-nitrophenyl-alpha-D-mannoside
(PubMed:12325362, PubMed:4973951, PubMed:1156387). Liberates
mannose from further alpha-D-mannosides including methyl-, benzyl-
alpha-D-mannoside, 1-6-linked di-, tri- and tetrasaccharides of
alpha-D-mannose and mannosyl-rhamnose (PubMed:12325362). Liberates
mannose from various glycoproteins like ovalbumin and ovomucoid
(PubMed:12325362, PubMed:5145). Does not hydrolyze beta-D-
mannosides (PubMed:12325362). Has glycosyltransferase activity,
forming disaccharides from mannose and lyxose but not from
glucose, galactose, ribose, xylose or arabinose (PubMed:12325362).
{ECO:0000269|PubMed:1156387, ECO:0000269|PubMed:12325362,
ECO:0000269|PubMed:4973951, ECO:0000269|PubMed:5145}.
-!- CATALYTIC ACTIVITY: Hydrolysis of terminal, non-reducing alpha-D-
mannose residues in alpha-D-mannosides.
{ECO:0000269|PubMed:1156387, ECO:0000269|PubMed:12325362,
ECO:0000269|PubMed:4973951, ECO:0000269|PubMed:5145}.
-!- COFACTOR:
Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Evidence={ECO:0000269|PubMed:1156387,
ECO:0000269|PubMed:21924285, ECO:0000269|PubMed:4973951};
Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:Q29451};
-!- ENZYME REGULATION: Inhibited by 2,3,4,6-tetra-O-acetyl-5-fluoro-
beta-L-gulopyranosyl fluoride which acts as a slow substrate,
doubling as a competitive inhibitor as it forms a high steady
state concentration of glycosyl-enzyme intermediate that blocks
the active site (PubMed:9442045). Inhibited by 2,3,4,6-tetra-O-
acetyl-5-fluoro-alpha-D-mannopyranosyl fluoride which also acts as
a slow substrate but no intermediates accumulate (PubMed:9442045).
Inhibited by EDTA (PubMed:4973951, PubMed:1156387,
PubMed:21924285). Inhibited by metal ion Cu(2+) (PubMed:4973951,
PubMed:1156387). Inhibited by metal ions Fe(2+), Cd(2+) and Co(2+)
(PubMed:4973951). Inhibited by metal ions Ag(+) and Hg(2+)
(PubMed:12325362, PubMed:4973951). Competitively inhibited by
mannono-1-4-lactone and mannono-1-5-lactone (PubMed:12325362).
Inhibited by swainsonine but not by 1-desoxymannojirimycin
(PubMed:21924285). Inhibited by pyrrolidine-3,4-diol derivatives
(PubMed:15974577). {ECO:0000269|PubMed:1156387,
ECO:0000269|PubMed:12325362, ECO:0000269|PubMed:15974577,
ECO:0000269|PubMed:21924285, ECO:0000269|PubMed:4973951,
ECO:0000269|PubMed:9442045}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=2.5 mM for p-nitrophenyl-alpha-D-mannoside
{ECO:0000269|PubMed:12325362};
KM=31 mM for benzyl-alpha-D-mannoside
{ECO:0000269|PubMed:12325362};
KM=0.12 M for methyl-alpha-D-mannoside
{ECO:0000269|PubMed:12325362};
KM=10.52 uM for 4-methylumbellideryl-alpha-mannoside
{ECO:0000269|PubMed:21924285};
pH dependence:
Optimum pH is 4-5 (PubMed:12325362, PubMed:5145,
PubMed:21924285). Activity is stable between pH 4.0 and pH 7.0
in the presence of Zn(2+) (PubMed:4973951). Activity is stable
between pH 6.0 and pH 8.5, decreases below pH 5.5 at 25 degrees
Celsius (PubMed:12325362). Activity is lost at pH 3 and lower,
rapidly diminishes at pH 7-10 but is higher and longest at pH
11-12 (PubMed:21924285). {ECO:0000269|PubMed:21924285,
ECO:0000269|PubMed:4973951, ECO:0000269|PubMed:5145};
Temperature dependence:
Retains 50% activity after 5 min at 70 degrees Celsius
(PubMed:12325362). Retains full activity after 5 min at 70
degrees Celsius but is completely inactive after 5 min at 80
degrees Celsius (PubMed:5145). Retains 100%, 50% and <10%
activity after 2 hours at 40, 55 and 70 degrees Celsius,
respectively (PubMed:21924285). {ECO:0000269|PubMed:12325362,
ECO:0000269|PubMed:21924285, ECO:0000269|PubMed:5145};
-!- SUBUNIT: Dimer of dimers of heavy and light subunits.
{ECO:0000269|PubMed:21924285, ECO:0000303|PubMed:5145}.
-!- SUBCELLULAR LOCATION: Protein storage vacuole
{ECO:0000269|PubMed:24221485}.
-!- DEVELOPMENTAL STAGE: Expressed from very early on in developing
cotyledons. {ECO:0000269|PubMed:24221485}.
-!- PTM: Produced as a precursor which is then proteolytically cleaved
into a 66kD heavy subunit and a 44kD light subunit. Cleavage
probably occurs in protein bodies/protein storage vacuoles.
{ECO:0000269|PubMed:24221485, ECO:0000269|PubMed:24295789,
ECO:0000269|PubMed:9442045, ECO:0000303|PubMed:24221485}.
-!- MASS SPECTROMETRY: Mass=66533; Method=Electrospray; Range=1-562;
Evidence={ECO:0000269|PubMed:9442045};
-!- MASS SPECTROMETRY: Mass=43846; Method=Electrospray; Range=563-981;
Evidence={ECO:0000269|PubMed:9442045};
-!- SIMILARITY: Belongs to the glycosyl hydrolase 38 family.
{ECO:0000255}.
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BindingDB; C0HJB3; -.
ChEMBL; CHEMBL3392945; -.
CAZy; GH38; Glycoside Hydrolase Family 38.
GlyConnect; 44; -.
iPTMnet; C0HJB3; -.
GO; GO:0000326; C:protein storage vacuole; IEA:UniProtKB-SubCell.
GO; GO:0004559; F:alpha-mannosidase activity; IEA:UniProtKB-EC.
GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0006013; P:mannose metabolic process; IEA:InterPro.
Gene3D; 1.20.1270.50; -; 2.
Gene3D; 2.60.40.1180; -; 1.
Gene3D; 3.20.110.10; -; 1.
InterPro; IPR011013; Gal_mutarotase_sf_dom.
InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
InterPro; IPR011682; Glyco_hydro_38_C.
InterPro; IPR015341; Glyco_hydro_38_cen.
InterPro; IPR037094; Glyco_hydro_38_cen_sf.
InterPro; IPR000602; Glyco_hydro_38_N.
InterPro; IPR027291; Glyco_hydro_38_N_sf.
InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
InterPro; IPR013780; Glyco_hydro_b.
Pfam; PF09261; Alpha-mann_mid; 1.
Pfam; PF01074; Glyco_hydro_38; 1.
Pfam; PF07748; Glyco_hydro_38C; 1.
SMART; SM00872; Alpha-mann_mid; 1.
SUPFAM; SSF74650; SSF74650; 1.
SUPFAM; SSF88688; SSF88688; 1.
SUPFAM; SSF88713; SSF88713; 1.
1: Evidence at protein level;
Direct protein sequencing; Disulfide bond; Glycoprotein; Glycosidase;
Hydrolase; Metal-binding; Vacuole; Zinc.
CHAIN 1 562 Alpha-mannosidase, heavy subunit.
{ECO:0000269|PubMed:24295789}.
/FTId=PRO_0000431307.
CHAIN 563 981 Alpha-mannosidase, light subunit.
{ECO:0000269|PubMed:24295789}.
/FTId=PRO_0000431308.
ACT_SITE 145 145 Nucleophile.
{ECO:0000269|PubMed:9442045}.
METAL 23 23 Zinc. {ECO:0000250|UniProtKB:Q29451}.
METAL 25 25 Zinc. {ECO:0000250|UniProtKB:Q29451}.
METAL 145 145 Zinc. {ECO:0000250|UniProtKB:Q29451}.
METAL 386 386 Zinc. {ECO:0000250|UniProtKB:Q29451}.
SITE 562 563 Cleavage; to produce subunits.
{ECO:0000269|PubMed:24295789}.
CARBOHYD 312 312 N-linked (GlcNAc...) asparagine.
{ECO:0000269|PubMed:24295789}.
CARBOHYD 446 446 N-linked (GlcNAc...) asparagine.
{ECO:0000269|PubMed:24295789}.
DISULFID 422 432 {ECO:0000269|PubMed:24295789}.
DISULFID 442 450 {ECO:0000269|PubMed:24295789}.
DISULFID 800 807 {ECO:0000269|PubMed:24295789}.
UNSURE 2 2 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 13 13 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 14 14 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 18 18 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 29 29 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 30 30 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 34 34 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 43 43 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 44 44 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 52 52 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 59 59 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 60 60 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 66 66 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 80 80 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 82 82 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 87 87 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 89 89 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 92 92 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 93 93 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 95 95 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 98 98 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 99 99 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 117 117 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 120 120 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 125 125 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 130 130 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 131 131 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 133 133 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 156 156 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 157 157 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 161 161 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 171 171 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 174 174 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 178 178 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 180 180 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 183 183 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 185 185 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 193 193 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 200 200 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 201 201 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 227 227 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 228 228 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 233 233 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 243 243 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 246 246 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 250 250 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 252 252 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 254 254 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 255 255 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 261 261 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 270 270 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 272 272 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 279 279 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 280 280 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 283 283 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 284 284 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 285 285 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 290 290 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 297 297 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 303 303 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 308 308 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 313 313 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 317 317 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 318 318 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 319 319 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 350 350 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 355 355 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 366 366 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 367 367 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 370 370 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 376 376 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 380 380 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 382 382 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 384 384 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 394 394 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 395 395 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 403 403 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 405 405 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 407 407 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 411 411 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 420 420 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 423 423 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 426 426 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 427 427 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 431 431 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 441 441 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 444 444 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 447 447 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 457 457 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 461 461 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 463 463 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 471 471 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 478 478 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 481 481 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 488 488 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 491 491 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 497 497 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 498 498 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 501 501 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 512 512 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 518 518 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 534 534 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 536 536 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 542 542 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 549 549 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 555 555 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 561 561 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 563 563 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 565 565 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 566 566 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 570 570 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 572 572 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 577 577 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 578 578 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 584 584 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 587 587 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 588 588 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 589 589 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 595 595 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 600 600 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 602 602 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 603 603 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 604 604 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 607 607 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 620 620 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 626 626 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 632 632 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 651 651 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 657 657 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 658 658 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 663 663 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 665 665 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 669 669 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 671 671 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 673 673 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 678 678 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 682 682 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 685 685 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 693 693 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 696 696 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 707 707 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 719 719 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 720 720 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 731 731 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 735 735 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 744 744 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 746 746 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 748 748 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 751 751 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 754 754 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 760 760 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 770 770 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 771 771 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 777 777 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 779 779 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 784 784 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 795 795 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 798 798 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 803 803 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 810 810 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 818 818 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 820 820 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 822 822 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 835 835 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 837 837 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 842 842 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 843 843 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 847 847 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 854 854 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 860 860 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 870 870 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 876 876 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 877 877 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 879 879 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 882 882 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 886 886 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 888 888 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 889 889 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 891 891 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 894 894 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 906 906 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 908 908 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 911 911 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 912 912 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 913 913 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 914 914 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 918 918 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 919 919 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 920 920 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 923 923 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 928 928 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 932 932 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 934 934 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 938 938 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 939 939 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 941 941 K or Q. {ECO:0000269|PubMed:24295789}.
UNSURE 950 950 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 953 953 Q or K. {ECO:0000269|PubMed:24295789}.
UNSURE 969 969 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 974 974 I or L. {ECO:0000269|PubMed:24295789}.
UNSURE 978 978 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 979 979 L or I. {ECO:0000269|PubMed:24295789}.
UNSURE 980 980 Q or K. {ECO:0000269|PubMed:24295789}.
SEQUENCE 981 AA; 111024 MW; 58C01389BDDCC20A CRC64;
MKYNTGAGTV PEQLNVHLVP HSHDDVGWLK TVDQYYVGSE NYIQEACVEN VLDSVVMSLQ
RDPNRKFVFG EMAFFHRWWL EQTPETKELX XKLVKAGQLE FVNGGWCMHD EATTHYIDMI
DHTTLGHRFL QEQFNKIPRA GWQIDPFGHS AVQGYLLGAE LGFDSVHFAR IDYQDREKRK
GEKSLEVVWR GSKTFGSSAQ IFANAFPGHY GPPNGFNFEV RNNFVPLQDD PRLFDTNVEE
RVQNFLDAAL TQAKLTRTNH LMWTMGDDFQ YQYAESWFKQ MDKLLHHVNK DGRVNALYST
PSLYTEAKNA ANQTWPLKID DYFPYADGRN AYWTGFYTSR XXXXXXXXML SGYYLATRHS
GFFAGKKSTK YHAFDLADAL GIAQHHDAVS GTAKQHTTND YAKRLALGAS KAEAVVSSSL
ACLTSKQSAD QCSAPASAFS QCHLFNISYC PPTESSLPDD KSLVVVVYNP LGWSRNEIVR
IPVNDANLVV KDSSGNKLEV QYVEMDDVTA NLRSFYVKXX XXXXXXXXXX YWSLFKASVP
PLGWSTYFIS EATGKGTRNA LTLSQKGETL NIGPGDLKMS FSSLTGQLKR MYNSKTGVDL
PIQQNYLWYE SSEGDFSDYQ ASGAYLFRPN GQPPPHTVSR SSVTRVTRGP LVDEVHQKFN
SWISQVTRLY KDKDHAEIEF TIGPIPTDDG VGKEVITRMT STMATNKEFY TDSNGRDFLK
RVRDYREDWP LEVTQPVAGN YYPLNLGLYT KDEKSEFSVL VDRATGGASI KDGEVELMLH
RRTLRDDGRG VGEPLDEQVC MNKEYTCEGL TVRGNYYLSI HKPAGGSRWR RTTGQEIYSP
MLLAFTQENM ENWKSSHSTK AYAMDPNYSL PPSVALITLE ELDDGLVLLR LAHLYEPSED
AEYSTLTKVE LKKLFATQKL EELREVSLSA NQEKSEMKKM KWSVEGDNEQ EPQAVRGGPV
SNADFVVELG PMEIRTFLLQ F


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