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Amino acid--[acyl-carrier-protein] ligase 1 (EC 6.2.1.n2) (Amino acid:[carrier-protein] ligase [AMP forming] 1) (aa:CP ligase 1) (Aminoacyl-[acyl-carrier-protein] synthetase 1) (L-glycine:[acyl-carrier-protein] ligase 1)
AACL1_BRADU Reviewed; 326 AA.
Q89VT8;
30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
01-JUN-2003, sequence version 1.
28-FEB-2018, entry version 83.
RecName: Full=Amino acid--[acyl-carrier-protein] ligase 1;
EC=6.2.1.n2;
AltName: Full=Amino acid:[carrier-protein] ligase [AMP forming] 1;
Short=aa:CP ligase 1;
AltName: Full=Aminoacyl-[acyl-carrier-protein] synthetase 1;
AltName: Full=L-glycine:[acyl-carrier-protein] ligase 1;
OrderedLocusNames=bll0957;
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC
14792 / USDA 110).
Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
Bradyrhizobiaceae; Bradyrhizobium.
NCBI_TaxID=224911;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110;
PubMed=12597275; DOI=10.1093/dnares/9.6.189;
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T.,
Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K.,
Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M.,
Tabata S.;
"Complete genomic sequence of nitrogen-fixing symbiotic bacterium
Bradyrhizobium japonicum USDA110.";
DNA Res. 9:189-197(2002).
[2]
X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) IN COMPLEXES WITH ATP; ZINC AND
SUBSTRATE ANALOG, FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY,
COFACTOR, KINETIC PARAMETERS, AND SUBUNIT.
STRAIN=JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110;
PubMed=20663952; DOI=10.1073/pnas.1007470107;
Mocibob M., Ivic N., Bilokapic S., Maier T., Luic M., Ban N.,
Weygand-Durasevic I.;
"Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and
provide a link between ribosomal and nonribosomal peptide synthesis.";
Proc. Natl. Acad. Sci. U.S.A. 107:14585-14590(2010).
-!- FUNCTION: Catalyzes the ATP-dependent activation of L-glycine and
its transfer to the phosphopantetheine prosthetic group covalently
attached to the vicinal carrier protein bsr0959 of yet unknown
function. May participate in nonribosomal peptide synthesis or
related processes. L-alanine is a poor substrate whereas L-serine
or D-amino acids are not substrates for ATP-dependent activation.
Does not display tRNA aminoacylation activity.
{ECO:0000269|PubMed:20663952}.
-!- CATALYTIC ACTIVITY: ATP + an amino acid + [acyl-carrier-protein] =
AMP + diphosphate + an aminoacyl-[acyl-carrier-protein].
{ECO:0000269|PubMed:20663952}.
-!- COFACTOR:
Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Evidence={ECO:0000269|PubMed:20663952};
Note=Binds 1 Zn(2+) ion per subunit.
{ECO:0000269|PubMed:20663952};
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=1.19 uM for carrier protein bsr0959
{ECO:0000269|PubMed:20663952};
KM=0.93 mM for L-glycine {ECO:0000269|PubMed:20663952};
KM=25 mM for L-alanine {ECO:0000269|PubMed:20663952};
Note=The catalytic efficiency of the glycine activation reaction
is 300-fold higher than that of alanine activation.;
-!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:20663952}.
-!- INTERACTION:
Q89VT6:bsr0959; NbExp=4; IntAct=EBI-16043152, EBI-16043178;
-!- MISCELLANEOUS: Lacks the N-terminal tRNA-binding domain compared
to class-II aminoacyl-tRNA synthetases.
-!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
family. Amino acid--[acyl-carrier-protein] ligase subfamily.
{ECO:0000305}.
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EMBL; BA000040; BAC46222.1; -; Genomic_DNA.
RefSeq; NP_767597.1; NC_004463.1.
RefSeq; WP_011083778.1; NZ_CP011360.1.
PDB; 3MEY; X-ray; 2.50 A; A/B=1-326.
PDB; 3MF1; X-ray; 2.20 A; A/B=1-326.
PDB; 3MF2; X-ray; 2.15 A; A/B=1-326.
PDB; 3PZC; X-ray; 2.20 A; A/B=1-326.
PDB; 4H2S; X-ray; 2.15 A; A/B=1-326.
PDB; 4H2T; X-ray; 2.44 A; A/B=1-326.
PDB; 4H2U; X-ray; 2.10 A; A/B=1-326.
PDB; 4H2V; X-ray; 2.00 A; A/B=1-326.
PDB; 4H2W; X-ray; 1.95 A; A/B=1-220, A/B=232-326.
PDB; 4H2X; X-ray; 2.15 A; A/B=1-220, A/B=232-326.
PDB; 4H2Y; X-ray; 2.10 A; A/B=1-220, A/B=232-326.
PDBsum; 3MEY; -.
PDBsum; 3MF1; -.
PDBsum; 3MF2; -.
PDBsum; 3PZC; -.
PDBsum; 4H2S; -.
PDBsum; 4H2T; -.
PDBsum; 4H2U; -.
PDBsum; 4H2V; -.
PDBsum; 4H2W; -.
PDBsum; 4H2X; -.
PDBsum; 4H2Y; -.
ProteinModelPortal; Q89VT8; -.
SMR; Q89VT8; -.
DIP; DIP-60151N; -.
IntAct; Q89VT8; 1.
STRING; 224911.bll0957; -.
EnsemblBacteria; BAC46222; BAC46222; BAC46222.
GeneID; 1051747; -.
KEGG; bja:bll0957; -.
PATRIC; fig|224911.44.peg.345; -.
eggNOG; ENOG41083GN; Bacteria.
eggNOG; COG0172; LUCA.
HOGENOM; HOG000177962; -.
InParanoid; Q89VT8; -.
OMA; VANDPFF; -.
OrthoDB; POG091H0L1Z; -.
PhylomeDB; Q89VT8; -.
BioCyc; BDIA224911:G1G3J-966-MONOMER; -.
BRENDA; 6.2.1.B4; 929.
SABIO-RK; Q89VT8; -.
EvolutionaryTrace; Q89VT8; -.
Proteomes; UP000002526; Chromosome.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
1: Evidence at protein level;
3D-structure; ATP-binding; Complete proteome; Ligase; Metal-binding;
Nucleotide-binding; Reference proteome; Zinc.
CHAIN 1 326 Amino acid--[acyl-carrier-protein] ligase
1.
/FTId=PRO_0000401186.
NP_BIND 168 169 ATP.
NP_BIND 250 253 ATP.
METAL 131 131 Zinc; catalytic.
METAL 176 176 Zinc; catalytic.
METAL 279 279 Zinc; catalytic.
BINDING 159 159 ATP.
BINDING 161 161 ATP.
BINDING 176 176 Amino acid substrate.
BINDING 235 235 ATP.
BINDING 286 286 ATP.
STRAND 12 19 {ECO:0000244|PDB:4H2W}.
HELIX 20 31 {ECO:0000244|PDB:4H2W}.
STRAND 36 39 {ECO:0000244|PDB:4H2W}.
HELIX 41 56 {ECO:0000244|PDB:4H2W}.
STRAND 62 66 {ECO:0000244|PDB:4H2W}.
STRAND 69 72 {ECO:0000244|PDB:4H2W}.
HELIX 73 79 {ECO:0000244|PDB:4H2W}.
HELIX 81 83 {ECO:0000244|PDB:4H2W}.
HELIX 86 88 {ECO:0000244|PDB:4H2W}.
STRAND 91 94 {ECO:0000244|PDB:4H2W}.
HELIX 99 110 {ECO:0000244|PDB:4H2W}.
HELIX 115 118 {ECO:0000244|PDB:4H2W}.
STRAND 120 126 {ECO:0000244|PDB:4H2W}.
STRAND 128 131 {ECO:0000244|PDB:4H2W}.
HELIX 134 139 {ECO:0000244|PDB:4H2W}.
STRAND 149 158 {ECO:0000244|PDB:4H2W}.
STRAND 170 181 {ECO:0000244|PDB:4H2W}.
HELIX 183 203 {ECO:0000244|PDB:4H2W}.
STRAND 209 212 {ECO:0000244|PDB:4H2W}.
HELIX 219 231 {ECO:0000244|PDB:4H2W}.
STRAND 235 240 {ECO:0000244|PDB:4H2W}.
STRAND 245 247 {ECO:0000244|PDB:4H2W}.
STRAND 249 256 {ECO:0000244|PDB:4H2W}.
HELIX 260 265 {ECO:0000244|PDB:4H2W}.
STRAND 272 274 {ECO:0000244|PDB:4H2V}.
STRAND 276 283 {ECO:0000244|PDB:4H2V}.
HELIX 285 287 {ECO:0000244|PDB:4H2W}.
HELIX 288 291 {ECO:0000244|PDB:4H2W}.
STRAND 292 297 {ECO:0000244|PDB:4H2W}.
HELIX 299 301 {ECO:0000244|PDB:4H2W}.
HELIX 304 310 {ECO:0000244|PDB:4H2W}.
SEQUENCE 326 AA; 35942 MW; 22DB7DAAD6AF36EF CRC64;
MNIAVLPNSP DTAPQIADPL DHLADKLFHS MGSDGVYART ALYESIVERL AALITSHREA
GTEALRFPPV MSRAQLEKSG YLKSFPNLLG CVCGLHGTER EINAAVSRFD AGGDWTTSLS
PADLVLSPAA CYPVYPIAAS RGPLPKGGLR FDVAADCFRR EPSKHLDRLQ SFRMREYVCI
GTPDDVSDFR ERWMVRAQAI ARDLGLTFRV DYASDPFFGR VGQMKAVSQK QQQLKFELLI
PLRSEEQPTA CMSFNYHREH FGTTWGIQDA NGEPAHTGCV AFGMDRLAVA MFHTHGTDLS
AWPAKVRDIL GLQPHVAAGA HGEGWR
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Pathways :
WP2199: Seed Development
WP1502: Mitochondrial biogenesis
WP1577: amino acid conjugation of benzoic acid
WP1625: Base excision repair
WP1672: Mismatch repair
WP1676: Non-homologous end-joining
WP1678: Nucleotide excision repair
WP211: BMP signaling pathway
WP521: amino acid conjugation of benzoic acid
WP2347: vitamin B5 (pantothenate) and CoA biosynthesis Pathway
WP1654: gamma-Hexachlorocyclohexane degradation
WP1700: Selenoamino acid metabolism
WP1996: Linoleate Biosynthesis
WP1659: Glycine, serine and threonine metabolism
WP1008: amino acid conjugation of benzoic acid
WP1049: G Protein Signaling Pathways
WP1097: Non-homologous end joining
WP1127: amino acid conjugation of benzoic acid
WP1165: G Protein Signaling Pathways
WP1201: Non-homologous end joining
WP1208: Non-homologous end joining
WP1222: Non-homologous end joining
WP1242: Non-homologous end joining
WP1252: amino acid conjugation of benzoic acid
WP1277: Non-homologous end joining
Related Genes :
[SLC38A1 ATA1 NAT2 SAT1 SNAT1] Sodium-coupled neutral amino acid transporter 1 (Amino acid transporter A1) (N-system amino acid transporter 2) (Solute carrier family 38 member 1) (System A amino acid transporter 1) (System N amino acid transporter 1)
[Slc38a1 Ata1 Glnt Sa2 Sat1 Snat1] Sodium-coupled neutral amino acid transporter 1 (Amino acid transporter A1) (rATA1) (Glutamine transporter) (N-system amino acid transporter 2) (Solute carrier family 38 member 1) (System A amino acid transporter 1) (System A transporter 2) (System N amino acid transporter 1)
[Slc38a1 Nat2 Snat1] Sodium-coupled neutral amino acid transporter 1 (Amino acid transporter A1) (MNat2) (N-system amino acid transporter 2) (Solute carrier family 38 member 1) (System A amino acid transporter 1) (System N amino acid transporter 1)
[Slc38a2 Ata2 Sa1 Sat2 Snat2] Sodium-coupled neutral amino acid transporter 2 (Amino acid transporter A2) (Solute carrier family 38 member 2) (System A amino acid transporter 2) (System A transporter 1) (System N amino acid transporter 2)
[SLC38A2 ATA2 KIAA1382 SAT2 SNAT2] Sodium-coupled neutral amino acid transporter 2 (Amino acid transporter A2) (Protein 40-9-1) (Solute carrier family 38 member 2) (System A amino acid transporter 2) (System A transporter 1) (System N amino acid transporter 2)
[Slc38a2 Ata2 Kiaa1382 Sat2 Snat2] Sodium-coupled neutral amino acid transporter 2 (Amino acid transporter A2) (Solute carrier family 38 member 2) (System A amino acid transporter 2) (System A transporter 1) (System N amino acid transporter 2)
[Slc38a4 Ata3 Nat3 Snat4] Sodium-coupled neutral amino acid transporter 4 (Amino acid transporter A3) (Na(+)-coupled neutral amino acid transporter 4) (Solute carrier family 38 member 4) (System A amino acid transporter 3)
[Slc36a1 Lyaat1 Pat1] Proton-coupled amino acid transporter 1 (Proton/amino acid transporter 1) (Lysosomal amino acid transporter 1) (LYAAT-1) (Neutral amino acid/proton symporter) (Solute carrier family 36 member 1)
[SLC38A3 G17 NAT1 SN1 SNAT3] Sodium-coupled neutral amino acid transporter 3 (N-system amino acid transporter 1) (Na(+)-coupled neutral amino acid transporter 3) (Solute carrier family 38 member 3) (System N amino acid transporter 1)
[Slc38a3 Sn1 Snat3] Sodium-coupled neutral amino acid transporter 3 (N-system amino acid transporter 1) (Na(+)-coupled neutral amino acid transporter 3) (Solute carrier family 38 member 3) (mNAT) (System N amino acid transporter 1)
[Slc38a3 Snat3] Sodium-coupled neutral amino acid transporter 3 (N-system amino acid transporter 1) (Na(+)-coupled neutral amino acid transporter 3) (Solute carrier family 38 member 3) (System N amino acid transporter 1)
[SLC38A4 ATA3 NAT3 SNAT4] Sodium-coupled neutral amino acid transporter 4 (Amino acid transporter A3) (Na(+)-coupled neutral amino acid transporter 4) (Solute carrier family 38 member 4) (System A amino acid transporter 3) (System N amino acid transporter 3)
[SLC7A5 CD98LC LAT1 MPE16] Large neutral amino acids transporter small subunit 1 (4F2 light chain) (4F2 LC) (4F2LC) (CD98 light chain) (Integral membrane protein E16) (L-type amino acid transporter 1) (hLAT1) (Solute carrier family 7 member 5) (y+ system cationic amino acid transporter)
[SLC7A7] Y+L amino acid transporter 1 (Monocyte amino acid permease 2) (MOP-2) (Solute carrier family 7 member 7) (y(+)L-type amino acid transporter 1) (Y+LAT1) (y+LAT-1)
[AAP1 NAT2 At1g58360 F19C14.3 ZCF54] Amino acid permease 1 (Amino acid transporter AAP1) (Neutral amino acid transporter II)
[SLC7A6 KIAA0245] Y+L amino acid transporter 2 (Cationic amino acid transporter, y+ system) (Solute carrier family 7 member 6) (y(+)L-type amino acid transporter 2) (Y+LAT2) (y+LAT-2)
[ARO8 YGL202W] Aromatic/aminoadipate aminotransferase 1 (2-aminoadipate aminotransferase) (EC 2.6.1.39) (2-aminoadipate transaminase) (Alpha-aminoadipate aminotransferase) (AadAT) (Aromatic amino acid aminotransferase 1) (EC 2.6.1.57) (Aromatic amino acid aminotransferase I) (Aromatic amino acid-requiring protein 8)
[bioF b0776 JW0759] 8-amino-7-oxononanoate synthase (AONS) (EC 2.3.1.47) (7-keto-8-amino-pelargonic acid synthase) (7-KAP synthase) (KAPA synthase) (8-amino-7-ketopelargonate synthase)
[BIOF BIO4 At5g04620 T32M21.220] 8-amino-7-oxononanoate synthase (AONS) (EC 2.3.1.47) (7-keto-8-amino-pelargonic acid synthase) (7-KAP synthase) (KAPA synthase) (8-amino-7-ketopelargonate synthase) (Biotin synthase 4) (Biotin synthase F) (AtbioF)
[bauA PA0132] Beta-alanine--pyruvate aminotransferase (Beta-A--Py AT) (EC 2.6.1.18) (Beta-alanine--pyruvate transaminase) (Omega-amino acid aminotransferase) (Omega-amino acid AT) (Omega-amino acid--pyruvate aminotransferase) (Omega-APT)
[TTHA1582] 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (AONS/AKB ligase) (EC 2.3.1.29) (EC 2.3.1.47) (7-keto-8-amino-pelargonic acid synthase) (7-KAP synthase) (KAPA synthase) (8-amino-7-ketopelargonate synthase) (Alpha-oxoamine synthase) (Glycine acetyltransferase)
[bioF1 Rv1569 MTCY336.34c] 8-amino-7-oxononanoate synthase 1 (AONS) (EC 2.3.1.47) (7-keto-8-amino-pelargonic acid synthase) (7-KAP synthase) (KAPA synthase) (8-amino-7-ketopelargonate synthase)
[SLC7A8 LAT2] Large neutral amino acids transporter small subunit 2 (L-type amino acid transporter 2) (hLAT2) (Solute carrier family 7 member 8)
[SLC7A9 BAT1] b(0,+)-type amino acid transporter 1 (b(0,+)AT1) (Glycoprotein-associated amino acid transporter b0,+AT1) (Solute carrier family 7 member 9)
[Slc7a8 Lat2 Lat4] Large neutral amino acids transporter small subunit 2 (L-type amino acid transporter 2) (Solute carrier family 7 member 8)
[SLC3A1 RBAT] Neutral and basic amino acid transport protein rBAT (NBAT) (D2h) (Solute carrier family 3 member 1) (b(0,+)-type amino acid transport protein)
[SLC1A5 ASCT2 M7V1 RDR RDRC] Neutral amino acid transporter B(0) (ATB(0)) (Baboon M7 virus receptor) (RD114/simian type D retrovirus receptor) (Sodium-dependent neutral amino acid transporter type 2) (Solute carrier family 1 member 5)
[SLC36A1 PAT1] Proton-coupled amino acid transporter 1 (Proton/amino acid transporter 1) (hPAT1) (Solute carrier family 36 member 1)
[SLC7A5] Large neutral amino acids transporter small subunit 1 (4F2 light chain) (4F2 LC) (4F2LC) (L-type amino acid transporter 1) (LAT1 light chain) (Solute carrier family 7 member 5)
[SLC7A2 ATRC2 CAT2] Cationic amino acid transporter 2 (CAT-2) (CAT2) (Low affinity cationic amino acid transporter 2) (Solute carrier family 7 member 2)
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