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Aminoglycoside 3'-phosphotransferase (EC 2.7.1.95) (APH(3')-I) (APH(3')I) (Kanamycin kinase, type I) (Neomycin-kanamycin phosphotransferase type I)

 KKA1_SALTM              Reviewed;         271 AA.
Q03447;
01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
01-JUN-1994, sequence version 1.
05-DEC-2018, entry version 71.
RecName: Full=Aminoglycoside 3'-phosphotransferase;
EC=2.7.1.95;
AltName: Full=APH(3')-I;
Short=APH(3')I;
AltName: Full=Kanamycin kinase, type I;
AltName: Full=Neomycin-kanamycin phosphotransferase type I;
Salmonella typhimurium.
Plasmid NTP16.
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Salmonella.
NCBI_TaxID=90371;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
TRANSPOSON=Tn4353;
PubMed=1325061; DOI=10.1016/0147-619X(92)90024-5;
Cannon P.M., Strike P.;
"Complete nucleotide sequence and gene organization of plasmid
NTP16.";
Plasmid 27:220-230(1992).
-!- FUNCTION: Resistance to kanamycin and structurally-related
aminoglycosides, including amikacin.
-!- CATALYTIC ACTIVITY:
Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
EC=2.7.1.95;
-!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase
family. {ECO:0000305}.
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EMBL; L05392; AAA72313.1; -; Genomic_DNA.
PIR; JQ1545; JQ1545.
RefSeq; NP_052154.1; NC_002090.1.
RefSeq; WP_010891090.1; NG_047435.1.
ProteinModelPortal; Q03447; -.
SMR; Q03447; -.
GeneID; 4267872; -.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
CDD; cd05150; APH; 1.
InterPro; IPR002575; Aminoglycoside_PTrfase.
InterPro; IPR024165; Kan/Strep_kinase.
InterPro; IPR011009; Kinase-like_dom_sf.
Pfam; PF01636; APH; 1.
PIRSF; PIRSF000706; Kanamycin_kin; 1.
SUPFAM; SSF56112; SSF56112; 1.
3: Inferred from homology;
Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding;
Plasmid; Transferase; Transposable element.
CHAIN 1 271 Aminoglycoside 3'-phosphotransferase.
/FTId=PRO_0000204803.
ACT_SITE 198 198 Proton acceptor. {ECO:0000250}.
SEQUENCE 271 AA; 30941 MW; 73A38B81CBF28917 CRC64;
MSHIQRETSC SRPRLNSNMD ADLYGYKWAR DNVGQSGATI DRLYGKPDAP ELFLKHGKGS
VANDVTDEMV RLNWMTEFMP LPTIKHFIRT PDDAWLLTTA IPGKTAFQVL EEYPDSGENI
VDALAVFLRR LHSIPVCNCP FNSDRVFRLV QAQSRMNNGL VDASDFDDER NGWPVEQVWK
EMHKLLPFSP DSVVTHGDFS LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS
PSLQKRLFQK YGIDNPDMNK LQFTLMLDEF F


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