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Ammonium transporter (Membrane protein NrgA) (Protein AmtB)

 AMTB_BACSU              Reviewed;         404 AA.
Q07429;
01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
01-OCT-1994, sequence version 1.
20-JUN-2018, entry version 128.
RecName: Full=Ammonium transporter {ECO:0000303|PubMed:14600241};
AltName: Full=Membrane protein NrgA {ECO:0000303|PubMed:8282685};
AltName: Full=Protein AmtB {ECO:0000303|PubMed:14600241};
Name=nrgA {ECO:0000303|PubMed:8282685};
Synonyms=amtB {ECO:0000303|PubMed:14600241};
OrderedLocusNames=BSU36510;
Bacillus subtilis (strain 168).
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
NCBI_TaxID=224308;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
PubMed=8282685; DOI=10.1128/jb.176.1.108-114.1994;
Wray L.V. Jr., Atkinson M.R., Fisher S.H.;
"The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a
membrane protein and a protein highly similar to the Escherichia coli
glnB-encoded PII protein.";
J. Bacteriol. 176:108-114(1994).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H.,
Villani G., Kunst F., Danchin A., Glaser P.;
"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
degrees).";
Microbiology 143:3313-3328(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
PubMed=9384377; DOI=10.1038/36786;
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus
subtilis.";
Nature 390:249-256(1997).
[4]
INDUCTION BY TNRA.
STRAIN=168;
PubMed=10864496; DOI=10.1006/jmbi.2000.3846;
Wray L.V. Jr., Zalieckas J.M., Fisher S.H.;
"Purification and in vitro activities of the Bacillus subtilis TnrA
transcription factor.";
J. Mol. Biol. 300:29-40(2000).
[5]
FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, AND SUBUNIT.
STRAIN=168;
PubMed=14600241; DOI=10.1099/mic.0.26512-0;
Detsch C., Stuelke J.;
"Ammonium utilization in Bacillus subtilis: transport and regulatory
functions of NrgA and NrgB.";
Microbiology 149:3289-3297(2003).
[6]
INDUCTION BY TNRA.
PubMed=12823818; DOI=10.1046/j.1365-2958.2003.03567.x;
Yoshida K., Yamaguchi H., Kinehara M., Ohki Y.-H., Nakaura Y.,
Fujita Y.;
"Identification of additional TnrA-regulated genes of Bacillus
subtilis associated with a TnrA box.";
Mol. Microbiol. 49:157-165(2003).
[7]
FUNCTION, INTERACTION WITH GLNK AND TNRA, AND DISRUPTION PHENOTYPE.
PubMed=17001076; DOI=10.1074/jbc.M607582200;
Heinrich A., Woyda K., Brauburger K., Meiss G., Detsch C., Stuelke J.,
Forchhammer K.;
"Interaction of the membrane-bound GlnK-AmtB complex with the master
regulator of nitrogen metabolism TnrA in Bacillus subtilis.";
J. Biol. Chem. 281:34909-34917(2006).
[8]
FUNCTION, INTERACTION WITH GLNK AND TNRA, AND DISRUPTION PHENOTYPE.
PubMed=21435182; DOI=10.1111/j.1742-4658.2011.08102.x;
Kayumov A., Heinrich A., Fedorova K., Ilinskaya O., Forchhammer K.;
"Interaction of the general transcription factor TnrA with the PII-
like protein GlnK and glutamine synthetase in Bacillus subtilis.";
FEBS J. 278:1779-1789(2011).
-!- FUNCTION: Functions as an ammonium and methylammonium transporter
in the absence of glutamine (PubMed:14600241). Required for
ammonium utilization at low concentrations or at low pH values,
when ammonium is the single nitrogen source (PubMed:14600241).
Required for binding of NrgB to the membrane (PubMed:14600241).
Interaction between GlnK-AmtB complex and TnrA protects TnrA from
proteolytic degradation (PubMed:17001076, PubMed:21435182).
{ECO:0000269|PubMed:14600241, ECO:0000269|PubMed:17001076,
ECO:0000269|PubMed:21435182}.
-!- SUBUNIT: Interacts with NrgB for a correct localization of the
latter. GlnK-AmtB complex interacts with TnrA.
{ECO:0000269|PubMed:17001076, ECO:0000269|PubMed:21435182,
ECO:0000305|PubMed:14600241}.
-!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:8282685};
Multi-pass membrane protein {ECO:0000305|PubMed:8282685}.
-!- INDUCTION: The nrgAB operon is activated by TnrA
(PubMed:12823818). NrgB is required for full induction of the
operon under conditions of ammonium limitation (PubMed:14600241,
PubMed:10864496). {ECO:0000269|PubMed:10864496,
ECO:0000269|PubMed:12823818, ECO:0000269|PubMed:14600241}.
-!- DISRUPTION PHENOTYPE: Cells grow poorly at pH 5.5 and not at all
at pH 5.0 with ammonium as their sole nitrogen source
(PubMed:14600241). They are unable to transport the ammonium
analog methylammonium, and thus probably also ammonium
(PubMed:14600241). In the absence of nitrogen source, cells
lacking this gene do not show degradation of TnrA, which is
entirely soluble (PubMed:17001076, PubMed:21435182).
{ECO:0000269|PubMed:14600241, ECO:0000269|PubMed:17001076,
ECO:0000269|PubMed:21435182}.
-!- SIMILARITY: Belongs to the ammonia transporter channel (TC
1.A.11.2) family. {ECO:0000305}.
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EMBL; L03216; AAA17399.1; -; Unassigned_DNA.
EMBL; Z82987; CAB05374.1; -; Genomic_DNA.
EMBL; AL009126; CAB15668.1; -; Genomic_DNA.
PIR; A36865; A36865.
RefSeq; NP_391532.1; NC_000964.3.
RefSeq; WP_003227757.1; NZ_JNCM01000034.1.
ProteinModelPortal; Q07429; -.
SMR; Q07429; -.
STRING; 224308.Bsubs1_010100019736; -.
PaxDb; Q07429; -.
EnsemblBacteria; CAB15668; CAB15668; BSU36510.
GeneID; 936933; -.
KEGG; bsu:BSU36510; -.
PATRIC; fig|224308.179.peg.3951; -.
eggNOG; ENOG4105BZU; Bacteria.
eggNOG; ENOG410XNMH; LUCA.
HOGENOM; HOG000017736; -.
InParanoid; Q07429; -.
KO; K03320; -.
OMA; AHMVWFW; -.
PhylomeDB; Q07429; -.
BioCyc; BSUB:BSU36510-MONOMER; -.
Proteomes; UP000001570; Chromosome.
GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
GO; GO:0008519; F:ammonium transmembrane transporter activity; IEA:InterPro.
Gene3D; 1.10.3430.10; -; 1.
InterPro; IPR029020; Ammonium/urea_transptr.
InterPro; IPR001905; Ammonium_transpt.
InterPro; IPR018047; Ammonium_transpt_CS.
InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
Pfam; PF00909; Ammonium_transp; 1.
TIGRFAMs; TIGR00836; amt; 1.
PROSITE; PS01219; AMMONIUM_TRANSP; 1.
1: Evidence at protein level;
Ammonia transport; Cell membrane; Complete proteome; Membrane;
Reference proteome; Transmembrane; Transmembrane helix; Transport.
CHAIN 1 404 Ammonium transporter.
/FTId=PRO_0000139760.
TRANSMEM 7 27 Helical. {ECO:0000255}.
TRANSMEM 44 64 Helical. {ECO:0000255}.
TRANSMEM 96 116 Helical. {ECO:0000255}.
TRANSMEM 125 145 Helical. {ECO:0000255}.
TRANSMEM 158 178 Helical. {ECO:0000255}.
TRANSMEM 227 247 Helical. {ECO:0000255}.
TRANSMEM 254 274 Helical. {ECO:0000255}.
TRANSMEM 277 297 Helical. {ECO:0000255}.
TRANSMEM 352 372 Helical. {ECO:0000255}.
SEQUENCE 404 AA; 42733 MW; 93A8C960ECAD2B9C CRC64;
MQMGDTVFMF FCALLVWLMT PGLALFYGGM VKSKNVLSTA MHSFSSIAIV SIVWVLFGYT
LAFAPGNSII GGLEWAGLKG VGFDPGDYSD TIPHSLFMMF QMTFAVLTTA IISGAFAERM
RFGAFLLFSV LWASLVYTPV AHWVWGGGWI GQLGALDFAG GNVVHISSGV AGLVLAIVLG
KRKDGTASSP HNLIYTFLGG ALIWFGWFGF NVGSALTLDG VAMYAFINTN TAAAAGIAGW
ILVEWIINKK PTMLGAVSGA IAGLVAITPA AGFVTPFASI IIGIIGGAVC FWGVFSLKKK
FGYDDALDAF GLHGIGGTWG GIATGLFATT SVNSAGADGL FYGDASLIWK QIVAIAATYV
FVFIVTFVII KIVSLFLPLR ATEEEESLGL DLTMHGEKAY QDSM


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