Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Ammonium transporter MEP1

 MEP1_YEAST              Reviewed;         492 AA.
P40260; D6VUQ2;
01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
01-FEB-1995, sequence version 1.
12-SEP-2018, entry version 152.
RecName: Full=Ammonium transporter MEP1;
Name=MEP1; Synonyms=AMT1; OrderedLocusNames=YGR121C; ORFNames=G6331;
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
NCBI_TaxID=559292;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Sigma 1278B;
PubMed=8062822;
Marini A.-M., Vissers S., Urrestarazu A., Andre B.;
"Cloning and expression of the MEP1 gene encoding an ammonium
transporter in Saccharomyces cerevisiae.";
EMBO J. 13:3456-3463(1994).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9046098;
DOI=10.1002/(SICI)1097-0061(199702)13:2<171::AID-YEA57>3.0.CO;2-V;
van Dyck L., Tettelin H., Purnelle B., Goffeau A.;
"An 18.3 kb DNA fragment from yeast chromosome VII carries four
unknown open reading frames, the gene for an Asn synthase, remnants of
Ty and three tRNA genes.";
Yeast 13:171-176(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169869;
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M.,
Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J.,
Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E.,
Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E.,
Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B.,
Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L.,
Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M.,
Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M.,
Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B.,
Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W.,
Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A.,
Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S.,
Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L.,
Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S.,
Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J.,
Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M.,
Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B.,
Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J.,
Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M.,
van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M.,
Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H.,
Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M.,
Zollner A., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
Nature 387:81-84(1997).
[4]
GENOME REANNOTATION.
STRAIN=ATCC 204508 / S288c;
PubMed=24374639; DOI=10.1534/g3.113.008995;
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
Cherry J.M.;
"The reference genome sequence of Saccharomyces cerevisiae: Then and
now.";
G3 (Bethesda) 4:389-398(2014).
[5]
FUNCTION.
STRAIN=Sigma 1278B;
PubMed=9234685; DOI=10.1128/MCB.17.8.4282;
Marini A.-M., Soussi-Boudekou S., Vissers S., Andre B.;
"A family of ammonium transporters in Saccharomyces cerevisiae.";
Mol. Cell. Biol. 17:4282-4293(1997).
[6]
FUNCTION.
PubMed=11486013; DOI=10.1128/MCB.21.17.5733-5741.2001;
Soupene E., Ramirez R.M., Kustu S.;
"Evidence that fungal MEP proteins mediate diffusion of the uncharged
species NH(3) across the cytoplasmic membrane.";
Mol. Cell. Biol. 21:5733-5741(2001).
[7]
TOPOLOGY [LARGE SCALE ANALYSIS].
STRAIN=ATCC 208353 / W303-1A;
PubMed=16847258; DOI=10.1073/pnas.0604075103;
Kim H., Melen K., Oesterberg M., von Heijne G.;
"A global topology map of the Saccharomyces cerevisiae membrane
proteome.";
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442 AND SER-445, AND
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
PubMed=19779198; DOI=10.1126/science.1172867;
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
"Global analysis of Cdk1 substrate phosphorylation sites provides
insights into evolution.";
Science 325:1682-1686(2009).
-!- FUNCTION: Transporter for ammonium (both charged and uncharged NH3
and NH4) to use as a nitrogen source. Can also transport
methylamine. The affinity of MEP1 is about twenty times lower than
that of MEP2. MEP3 has the lowest affinity.
{ECO:0000269|PubMed:11486013, ECO:0000269|PubMed:9234685}.
-!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
-!- SIMILARITY: Belongs to the ammonia transporter channel (TC
1.A.11.2) family. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; X77608; CAA54699.1; -; Genomic_DNA.
EMBL; X83099; CAA58156.1; -; Genomic_DNA.
EMBL; Z72906; CAA97132.1; -; Genomic_DNA.
EMBL; BK006941; DAA08213.1; -; Genomic_DNA.
PIR; S46225; S46225.
RefSeq; NP_011636.3; NM_001181250.3.
ProteinModelPortal; P40260; -.
BioGrid; 33367; 132.
DIP; DIP-5530N; -.
IntAct; P40260; 13.
MINT; P40260; -.
STRING; 4932.YGR121C; -.
TCDB; 1.A.11.3.1; the ammonium transporter channel (amt) family.
iPTMnet; P40260; -.
PaxDb; P40260; -.
PRIDE; P40260; -.
EnsemblFungi; YGR121C; YGR121C; YGR121C.
GeneID; 853019; -.
KEGG; sce:YGR121C; -.
EuPathDB; FungiDB:YGR121C; -.
SGD; S000003353; MEP1.
GeneTree; ENSGT00530000064546; -.
HOGENOM; HOG000017736; -.
InParanoid; P40260; -.
KO; K03320; -.
OMA; ECVNYTG; -.
OrthoDB; EOG092C1Z22; -.
BioCyc; YEAST:G3O-30828-MONOMER; -.
PRO; PR:P40260; -.
Proteomes; UP000002311; Chromosome VII.
GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
GO; GO:0005886; C:plasma membrane; IDA:SGD.
GO; GO:0008519; F:ammonium transmembrane transporter activity; IDA:SGD.
GO; GO:0015696; P:ammonium transport; IMP:SGD.
GO; GO:0019740; P:nitrogen utilization; IMP:SGD.
Gene3D; 1.10.3430.10; -; 1.
InterPro; IPR029020; Ammonium/urea_transptr.
InterPro; IPR001905; Ammonium_transpt.
InterPro; IPR018047; Ammonium_transpt_CS.
InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
Pfam; PF00909; Ammonium_transp; 1.
TIGRFAMs; TIGR00836; amt; 1.
PROSITE; PS01219; AMMONIUM_TRANSP; 1.
1: Evidence at protein level;
Ammonia transport; Complete proteome; Membrane; Phosphoprotein;
Reference proteome; Transmembrane; Transmembrane helix; Transport.
CHAIN 1 492 Ammonium transporter MEP1.
/FTId=PRO_0000139754.
TOPO_DOM 1 18 Extracellular. {ECO:0000255}.
TRANSMEM 19 39 Helical. {ECO:0000255}.
TOPO_DOM 40 49 Cytoplasmic. {ECO:0000255}.
TRANSMEM 50 70 Helical. {ECO:0000255}.
TOPO_DOM 71 109 Extracellular. {ECO:0000255}.
TRANSMEM 110 130 Helical. {ECO:0000255}.
TOPO_DOM 131 140 Cytoplasmic. {ECO:0000255}.
TRANSMEM 141 161 Helical. {ECO:0000255}.
TOPO_DOM 162 174 Extracellular. {ECO:0000255}.
TRANSMEM 175 195 Helical. {ECO:0000255}.
TOPO_DOM 196 210 Cytoplasmic. {ECO:0000255}.
TRANSMEM 211 231 Helical. {ECO:0000255}.
TOPO_DOM 232 240 Extracellular. {ECO:0000255}.
TRANSMEM 241 261 Helical. {ECO:0000255}.
TOPO_DOM 262 268 Cytoplasmic. {ECO:0000255}.
TRANSMEM 269 289 Helical. {ECO:0000255}.
TOPO_DOM 290 290 Extracellular. {ECO:0000255}.
TRANSMEM 291 311 Helical. {ECO:0000255}.
TOPO_DOM 312 331 Cytoplasmic. {ECO:0000255}.
TRANSMEM 332 352 Helical. {ECO:0000255}.
TOPO_DOM 353 373 Extracellular. {ECO:0000255}.
TRANSMEM 374 394 Helical. {ECO:0000255}.
TOPO_DOM 395 492 Cytoplasmic. {ECO:0000255}.
MOD_RES 442 442 Phosphoserine.
{ECO:0000244|PubMed:19779198}.
MOD_RES 445 445 Phosphoserine.
{ECO:0000244|PubMed:19779198}.
SEQUENCE 492 AA; 54202 MW; CC7DF0EB838D0B77 CRC64;
MESRTTGPLT TETYDGPTVA FMILGAALVF FMVPGLGFLY SGLARRKSAL ALIWVVLMAT
LVGILQWYFW GYSLAFSKSA PNNKFIGNLD SFGFRNVYGK KFDEDAYPEL AYATFQMMFS
CVNLSIIAGA TAERGRLLPH MVFLFILATI GYCPVTYWIW SPGGWAYQWG VLDWAGGGNI
EILSAVSGFV YSWFLGKRNE KLLINFRPHN VSLVTLGTSI LWFGWLLFNS ASSLSPNLRS
VYAFMNTCLS AITGGMTWCL LDYRSEKKWS TVGLCSGIIS GLVAATPSSG CITLYGSLIQ
GIVAGVVCNF ATKLKYYAKV DDAMDILAEH GVAGVIGLIF NALFGADWVI GMDGTTEHEG
GWVTHNYKQM YKQIAYIAAS IGYTAAVTAI ICFVLGYIPG MRLRISEEAE EAGMDEDQIG
EFAYDYVEVR RDYYLWGVDE DSQRSDVNHR VNNAHLAAER SSSGTNSSSD GNGEMIQSEK
ILPIHQEDPA NR


Related products :

Catalog number Product name Quantity
orb140433 Ammonium transporter (Phospho-Thr465) antibody Polyclonal Rabbit Polyclonal to Ammonium transporter (Phospho-Thr465). 5
AP3770a-ev20 Ammonium transporter
E0021h Rat ELISA Kit FOR Ammonium transporter Rh type C 96T
E1667m Rat ELISA Kit FOR Ammonium transporter Rh type B 96T
RHCG_PIG Pig ELISA Kit FOR Ammonium transporter Rh type C 96T
USBP1_MOUSE Rat ELISA Kit FOR Ammonium transporter Rh type C 96T
ARC1A_HUMAN Rat ELISA Kit FOR Ammonium transporter Rh type C 96T
E0794r Human ELISA Kit FOR Ammonium transporter Rh type A 96T
CSB-EL019674RA Rat Ammonium transporter Rh type B(RHBG) ELISA kit 96T
CSB-EL019665RA Rat Ammonium transporter Rh type A(RHAG) ELISA kit 96T
orb140433 Ammonium transporter (Phospho-Thr465) antibody 20 ug(Trial size)
CSB-EL019665DO Dog Ammonium transporter Rh type A(RHAG) ELISA kit 96T
CSB-EL019676RA Rat Ammonium transporter Rh type C(RHCG) ELISA kit 96T
orb140433 Ammonium transporter (Phospho-Thr465) antibody 5 ug(Trial size)
orb140433 Ammonium transporter (Phospho-Thr465) antibody 100 ug
RHAG_BOVIN Bovine ELISA Kit FOR Ammonium transporter Rh type A 96T
RHBG_BOVIN Bovine ELISA Kit FOR Ammonium transporter Rh type B 96T
E1901h Human ELISA Kit FOR Ammonium transporter Rh type B 96T
CSB-EL019676DO Dog Ammonium transporter Rh type C(RHCG) ELISA kit 96T
CSB-EL019674DO Dog Ammonium transporter Rh type B(RHBG) ELISA kit 96T
AP3770a Phospho-Ammonium transporter-T465 Antibody
CSB-EL019665RA Rat Ammonium transporter Rh type A(RHAG) ELISA kit SpeciesRat 96T
CSB-EL019674RA Rat Ammonium transporter Rh type B(RHBG) ELISA kit SpeciesRat 96T
CSB-EL019676RA Rat Ammonium transporter Rh type C(RHCG) ELISA kit SpeciesRat 96T
CSB-EL019665BO Bovine Ammonium transporter Rh type A(RHAG) ELISA kit 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur