GENTAUR Molecular Products.

 RSHA_MYCS2              Reviewed;         101 AA.
 A0QTP3; Q9XCD7;
 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
 09-JAN-2007, sequence version 1.
 20-JUN-2018, entry version 67.
 RecName: Full=Anti-sigma factor RshA;
 AltName: Full=Regulator of SigH;
 AltName: Full=Sigma-H anti-sigma factor RshA;
 Name=rshA; OrderedLocusNames=MSMEG_1915, MSMEI_1875;
 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155).
 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
 Mycolicibacterium.
 NCBI_TaxID=246196;
 [1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
 STRAIN=ATCC 700084 / mc(2)155;
 PubMed=10400584;
 Fernandes N.D., Wu Q.-L., Kong D., Puyang X., Garg S., Husson R.N.;
 "A mycobacterial extracytoplasmic sigma factor involved in survival
 following heat shock and oxidative stress.";
 J. Bacteriol. 181:4266-4274(1999).
 [2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 STRAIN=ATCC 700084 / mc(2)155;
 Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
 Fraser C.M.;
 Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
 [3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 STRAIN=ATCC 700084 / mc(2)155;
 PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
 Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
 Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
 "Interrupted coding sequences in Mycobacterium smegmatis: authentic
 mutations or sequencing errors?";
 Genome Biol. 8:R20.1-R20.9(2007).
 [4]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 STRAIN=ATCC 700084 / mc(2)155;
 PubMed=18955433; DOI=10.1101/gr.081901.108;
 Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
 Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
 "Ortho-proteogenomics: multiple proteomes investigation through
 orthology and a new MS-based protocol.";
 Genome Res. 19:128-135(2009).
 [5]
 PROBABLE INTERACTION WITH SIGH, AND MUTAGENESIS OF CYS-25; CYS-40;
 CYS-55; CYS-58 AND CYS-76.
 STRAIN=ATCC 700084 / mc(2)155;
 PubMed=14617153; DOI=10.1046/j.1365-2958.2003.03739.x;
 Song T., Dove S.L., Lee K.H., Husson R.N.;
 "RshA, an anti-sigma factor that regulates the activity of the
 mycobacterial stress response sigma factor SigH.";
 Mol. Microbiol. 50:949-959(2003).
 [6]
 POSSIBLE PHOSPHORYLATION, AND REGULATION.
 PubMed=18728196; DOI=10.1073/pnas.0801143105;
 Park S.T., Kang C.M., Husson R.N.;
 "Regulation of the SigH stress response regulon by an essential
 protein kinase in Mycobacterium tuberculosis.";
 Proc. Natl. Acad. Sci. U.S.A. 105:13105-13110(2008).
 -!- FUNCTION: A redox-regulated anti-sigma factor for cognate
     extracytoplasmic function (ECF) sigma factor SigH. ECF sigma
     factors are held in an inactive form by an anti-sigma factor
     (Probable). Overexpression leads to increased susceptibility to
     diamide. {ECO:0000305}.
 -!- COFACTOR:
     Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
       Evidence={ECO:0000305};
     Note=Binds 1 iron-sulfur cluster per subunit. {ECO:0000305};
 -!- SUBUNIT: Interacts with cognate ECF RNA polymerase sigma factor
     SigH under reducing conditions; the complex is disrupted under
     oxiding conditions or as temperatures rise. Binding inhibits the
     interaction of SigH with the RNA polymerase catalytic core.
 -!- INDUCTION: Induced after heat shock (50 degress Celsius). Part of
     the sigH-rshA operon. {ECO:0000269|PubMed:10400584}.
 -!- PTM: Phosphorylated, probably by PknB (Probable). Phosphorylation
     decreases interaction with SigH, probably leading to increased
     SigH-mediated transcription. {ECO:0000305}.
 -!- SIMILARITY: Belongs to the zinc-associated anti-sigma factor (ZAS)
     superfamily. {ECO:0000305}.
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 EMBL; AF144091; AAD41811.1; -; Genomic_DNA.
 EMBL; CP000480; ABK70150.1; -; Genomic_DNA.
 EMBL; CP001663; AFP38347.1; -; Genomic_DNA.
 RefSeq; WP_011728007.1; NZ_CP009494.1.
 RefSeq; YP_886281.1; NC_008596.1.
 ProteinModelPortal; A0QTP3; -.
 STRING; 246196.MSMEG_1915; -.
 EnsemblBacteria; ABK70150; ABK70150; MSMEG_1915.
 EnsemblBacteria; AFP38347; AFP38347; MSMEI_1875.
 GeneID; 4535149; -.
 KEGG; msb:LJ00_09560; -.
 KEGG; msg:MSMEI_1875; -.
 KEGG; msm:MSMEG_1915; -.
 PATRIC; fig|246196.19.peg.1897; -.
 eggNOG; ENOG4105WMN; Bacteria.
 eggNOG; ENOG41128S4; LUCA.
 HOGENOM; HOG000247360; -.
 OMA; PCLEKYG; -.
 OrthoDB; POG091H09CC; -.
 Proteomes; UP000000757; Chromosome.
 Proteomes; UP000006158; Chromosome.
 GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
 GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-KW.
 GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
 InterPro; IPR024020; Anit_sigma_mycothiol_RsrA.
 InterPro; IPR014295; Anti-sigma.
 InterPro; IPR027383; Znf_put.
 Pfam; PF13490; zf-HC2; 1.
 TIGRFAMs; TIGR02949; anti_SigH_actin; 1.
 TIGRFAMs; TIGR03988; antisig_RsrA; 1.
 1: Evidence at protein level;
 Complete proteome; Iron; Iron-sulfur; Metal-binding; Phosphoprotein;
 Reference proteome; Transcription; Transcription regulation.
 CHAIN         1    101       Anti-sigma factor RshA.
                              /FTId=PRO_0000423651.
 METAL        25     25       Iron-sulfur. {ECO:0000255}.
 METAL        51     51       Iron-sulfur. {ECO:0000255}.
 METAL        55     55       Iron-sulfur. {ECO:0000250}.
 METAL        58     58       Iron-sulfur. {ECO:0000250}.
 MOD_RES      96     96       Phosphothreonine. {ECO:0000250}.
 MUTAGEN      25     25       C->A: Slightly decreased inhibition of
                              SigH. {ECO:0000269|PubMed:14617153}.
 MUTAGEN      40     40       C->A: No effect.
                              {ECO:0000269|PubMed:14617153}.
 MUTAGEN      55     55       C->A: Decreased inhibition of SigH;
                              reduced interaction with SigH.
                              {ECO:0000269|PubMed:14617153}.
 MUTAGEN      58     58       C->A: Decreased inhibition of SigH;
                              reduced interaction with SigH.
                              {ECO:0000269|PubMed:14617153}.
 MUTAGEN      76     76       C->A: No effect.
                              {ECO:0000269|PubMed:14617153}.
 SEQUENCE   101 AA;  11606 MW;  974265FA55D88790 CRC64;
 MSETEREDER WTPPIGPIDP EHPECAAVIA EVWTLLDGEC TPETRDKLKQ HLEECPTCLR
 HYGIEERVKR LIAAKCSGEK APDSLRERLR IQISRTTIIR G

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