Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Aspartate 1-decarboxylase (EC 4.1.1.11) (Aspartate alpha-decarboxylase) [Cleaved into: Aspartate 1-decarboxylase beta chain; Aspartate 1-decarboxylase alpha chain]

 PAND_MYCTA              Reviewed;         139 AA.
A5U8S6;
02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
10-JUL-2007, sequence version 1.
22-NOV-2017, entry version 64.
RecName: Full=Aspartate 1-decarboxylase {ECO:0000255|HAMAP-Rule:MF_00446};
EC=4.1.1.11 {ECO:0000255|HAMAP-Rule:MF_00446};
AltName: Full=Aspartate alpha-decarboxylase {ECO:0000255|HAMAP-Rule:MF_00446};
Contains:
RecName: Full=Aspartate 1-decarboxylase beta chain {ECO:0000255|HAMAP-Rule:MF_00446};
Contains:
RecName: Full=Aspartate 1-decarboxylase alpha chain {ECO:0000255|HAMAP-Rule:MF_00446};
Flags: Precursor;
Name=panD {ECO:0000255|HAMAP-Rule:MF_00446};
OrderedLocusNames=MRA_3640;
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra).
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
NCBI_TaxID=419947;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 25177 / H37Ra;
PubMed=18584054; DOI=10.1371/journal.pone.0002375;
Zheng H., Lu L., Wang B., Pu S., Zhang X., Zhu G., Shi W., Zhang L.,
Wang H., Wang S., Zhao G., Zhang Y.;
"Genetic basis of virulence attenuation revealed by comparative
genomic analysis of Mycobacterium tuberculosis strain H37Ra versus
H37Rv.";
PLoS ONE 3:E2375-E2375(2008).
[2]
FUNCTION, ENZYME REGULATION, POSSIBLE ANTIBIOTIC RESISTANCE, AND
MUTAGENESIS OF MET-117; 127-ASN--GLY-139; LEU-136 AND VAL-138.
STRAIN=ATCC 25177 / H37Ra;
PubMed=26038753; DOI=10.1038/emi.2014.61;
Shi W., Chen J., Feng J., Cui P., Zhang S., Weng X., Zhang W.,
Zhang Y.;
"Aspartate decarboxylase (PanD) as a new target of pyrazinamide in
Mycobacterium tuberculosis.";
Emerg. Microbes Infect. 3:E58-E58(2014).
-!- FUNCTION: Catalyzes the pyruvoyl-dependent decarboxylation of
aspartate to produce beta-alanine. {ECO:0000255|HAMAP-
Rule:MF_00446}.
-!- FUNCTION: Overexpression of wild-type or mutant proteins confers
resistance to pyrazinoic acid (POA), the active form of the anti-
tuberculosis prodrug pyrazinamide (PZA), when grown on agar
plates. {ECO:0000269|PubMed:26038753}.
-!- CATALYTIC ACTIVITY: L-aspartate = beta-alanine + CO(2).
{ECO:0000255|HAMAP-Rule:MF_00446}.
-!- COFACTOR:
Name=pyruvate; Xref=ChEBI:CHEBI:15361;
Evidence={ECO:0000255|HAMAP-Rule:MF_00446};
Note=Binds 1 pyruvoyl group covalently per subunit.
{ECO:0000255|HAMAP-Rule:MF_00446};
-!- ENZYME REGULATION: Partially inhibited by POA but not by PZA or
nicotinamide, probably also inhibited by calcium pantothenate.
{ECO:0000269|PubMed:26038753}.
-!- PATHWAY: Cofactor biosynthesis; (R)-pantothenate biosynthesis;
beta-alanine from L-aspartate: step 1/1. {ECO:0000255|HAMAP-
Rule:MF_00446}.
-!- SUBUNIT: Heterooctamer of four alpha and four beta subunits.
{ECO:0000255|HAMAP-Rule:MF_00446}.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00446}.
-!- PTM: Is synthesized initially as an inactive proenzyme, which is
activated by self-cleavage at a specific serine bond to produce a
beta-subunit with a hydroxyl group at its C-terminus and an alpha-
subunit with a pyruvoyl group at its N-terminus.
{ECO:0000255|HAMAP-Rule:MF_00446}.
-!- MISCELLANEOUS: 30 POA or PZA-resistant mutants were identified in
this gene by selection at pH 5.7 - 6.8. The antituberculosis
activity of POA is antagonized by beta-alanine and pantothenate,
the immediate and final reaction products of this enzyme
(PubMed:26038753). Experiments in strain H37Rv suggest however
that the PZA target may not be PanD (By similarity).
{ECO:0000250|UniProtKB:P9WIL3, ECO:0000269|PubMed:26038753}.
-!- SIMILARITY: Belongs to the PanD family. {ECO:0000255|HAMAP-
Rule:MF_00446}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; CP000611; ABQ75426.1; -; Genomic_DNA.
RefSeq; WP_003419523.1; NZ_CP016972.1.
ProteinModelPortal; A5U8S6; -.
SMR; A5U8S6; -.
STRING; 419947.MtubH3_010100009274; -.
EnsemblBacteria; ABQ75426; ABQ75426; MRA_3640.
KEGG; mra:MRA_3640; -.
eggNOG; ENOG4108Z2X; Bacteria.
eggNOG; COG0853; LUCA.
HOGENOM; HOG000221007; -.
KO; K01579; -.
OMA; LYSKIHR; -.
UniPathway; UPA00028; UER00002.
Proteomes; UP000001988; Chromosome.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0004068; F:aspartate 1-decarboxylase activity; IEA:UniProtKB-EC.
GO; GO:0006523; P:alanine biosynthetic process; IEA:InterPro.
GO; GO:0015940; P:pantothenate biosynthetic process; IEA:UniProtKB-UniPathway.
GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
CDD; cd06919; Asp_decarbox; 1.
HAMAP; MF_00446; PanD; 1.
InterPro; IPR009010; Asp_de-COase-like_dom_sf.
InterPro; IPR003190; Asp_decarbox.
PANTHER; PTHR21012; PTHR21012; 1.
Pfam; PF02261; Asp_decarbox; 1.
PIRSF; PIRSF006246; Asp_decarbox; 1.
ProDom; PD009294; Asp_decarbox; 1.
SUPFAM; SSF50692; SSF50692; 1.
TIGRFAMs; TIGR00223; panD; 1.
1: Evidence at protein level;
Antibiotic resistance; Autocatalytic cleavage; Complete proteome;
Cytoplasm; Decarboxylase; Lyase; Pantothenate biosynthesis; Pyruvate;
Schiff base; Zymogen.
CHAIN 1 24 Aspartate 1-decarboxylase beta chain.
{ECO:0000255|HAMAP-Rule:MF_00446}.
/FTId=PRO_0000307029.
CHAIN 25 139 Aspartate 1-decarboxylase alpha chain.
{ECO:0000255|HAMAP-Rule:MF_00446}.
/FTId=PRO_0000307030.
REGION 73 75 Substrate binding. {ECO:0000255|HAMAP-
Rule:MF_00446}.
ACT_SITE 25 25 Schiff-base intermediate with substrate;
via pyruvic acid. {ECO:0000255|HAMAP-
Rule:MF_00446}.
ACT_SITE 58 58 Proton donor. {ECO:0000255|HAMAP-
Rule:MF_00446}.
BINDING 57 57 Substrate. {ECO:0000255|HAMAP-
Rule:MF_00446}.
MOD_RES 25 25 Pyruvic acid (Ser).
MUTAGEN 117 117 M->I: Confers POA resistance, partially
inhibited by POA; identified in 24/30
mutants. {ECO:0000269|PubMed:26038753}.
MUTAGEN 127 139 Missing: Confers POA resistance.
{ECO:0000269|PubMed:26038753}.
MUTAGEN 136 136 L->R: Confers POA resistance.
{ECO:0000269|PubMed:26038753}.
MUTAGEN 138 138 V->A,E,G: Confers POA resistance.
{ECO:0000269|PubMed:26038753}.
SEQUENCE 139 AA; 14885 MW; C5BFDC1C996ED9C6 CRC64;
MLRTMLKSKI HRATVTCADL HYVGSVTIDA DLMDAADLLE GEQVTIVDID NGARLVTYAI
TGERGSGVIG INGAAAHLVH PGDLVILIAY ATMDDARART YQPRIVFVDA YNKPIDMGHD
PAFVPENAGE LLDPRLGVG


Related products :

Catalog number Product name Quantity
U1258h CLIA 67 kDa glutamic acid decarboxylase,GAD,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Homo sapiens,Human 96T
E1258h ELISA 67 kDa glutamic acid decarboxylase,GAD,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Homo sapiens,Human 96T
E1258h ELISA kit 67 kDa glutamic acid decarboxylase,GAD,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Homo sapiens,Human 96T
E1258r ELISA 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Rat,Rattus norvegicus 96T
E1258m ELISA kit 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Mouse,Mus musculus 96T
E1258m ELISA 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Mouse,Mus musculus 96T
U1258r CLIA 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Rat,Rattus norvegicus 96T
E1258r ELISA kit 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Rat,Rattus norvegicus 96T
U1258m CLIA 67 kDa glutamic acid decarboxylase,Gad1,Gad67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Mouse,Mus musculus 96T
EIAAB09546 CSAD,CSD,Cysteine sulfinic acid decarboxylase,Cysteine-sulfinate decarboxylase,Homo sapiens,Human,Sulfinoalanine decarboxylase
EIAAB10595 65 kDa glutamic acid decarboxylase,GAD2,GAD65,GAD-65,Glutamate decarboxylase 2,Glutamate decarboxylase 65 kDa isoform,Homo sapiens,Human
E1258p ELISA kit 67 kDa glutamic acid decarboxylase,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Pig,Sus scrofa 96T
18-783-77682 RABBIT ANTI HUMAN GAD 65 - GLUTAMATE DECARBOXYLASE; EC 4.1.1.15; Glutamate decarboxylase 67 kDa isoform; GAD-67; 67 kDa glutamic acid decarboxylase Polyclonal 1 ml
EIAAB10594 65 kDa glutamic acid decarboxylase,Gad2,Gad65,GAD-65,Glutamate decarboxylase 2,Glutamate decarboxylase 65 kDa isoform,Mouse,Mus musculus
EIAAB25871 Diphosphomevalonate decarboxylase,Homo sapiens,Human,MDDase,Mevalonate (diphospho)decarboxylase,Mevalonate pyrophosphate decarboxylase,MPD,MVD
EIAAB10592 65 kDa glutamic acid decarboxylase,Gad2,Gad65,GAD-65,Glutamate decarboxylase 2,Glutamate decarboxylase 65 kDa isoform,Rat,Rattus norvegicus
EIAAB09547 Csad,Cysteine sulfinic acid decarboxylase,Cysteine-sulfinate decarboxylase,Mouse,Mus musculus,Sulfinoalanine decarboxylase
U1258p CLIA 67 kDa glutamic acid decarboxylase,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Pig,Sus scrofa 96T
EIAAB09548 Csad,Csd,Cysteine sulfinic acid decarboxylase,Cysteine-sulfinate decarboxylase,Rat,Rattus norvegicus,Sulfinoalanine decarboxylase
E1258p ELISA 67 kDa glutamic acid decarboxylase,GAD1,GAD67,GAD-67,Glutamate decarboxylase 1,Glutamate decarboxylase 67 kDa isoform,Pig,Sus scrofa 96T
EIAAB25869 Diphosphomevalonate decarboxylase,MDDase,Mevalonate (diphospho)decarboxylase,Mevalonate pyrophosphate decarboxylase,Mouse,Mus musculus,Mvd
EIAAB25868 Diphosphomevalonate decarboxylase,MDDase,Mevalonate (diphospho)decarboxylase,Mevalonate pyrophosphate decarboxylase,Mpd,Mvd,Rat,Rattus norvegicus
EIAAB25870 Bos taurus,Bovine,Diphosphomevalonate decarboxylase,MDDase,Mevalonate (diphospho)decarboxylase,Mevalonate pyrophosphate decarboxylase,MVD
EIAAB10591 65 kDa glutamic acid decarboxylase,GAD2,GAD65,GAD-65,Glutamate decarboxylase 2,Glutamate decarboxylase 65 kDa isoform,Pig,Sus scrofa
18-783-77681 RABBIT ANTI HUMAN GLUTAMATE DECARBOXYLASE - GLUTAMATE DEHYDROGENASE; EC 4.1.1.15; Glutamate decarboxylase 67 kDa isoform; GAD-67; 67 kDa glutamic acid decarboxylase Polyclonal 0.05 ml


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur