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Beta sliding clamp (Beta clamp) (Sliding clamp) (Beta-clamp processivity factor) (DNA polymerase III beta sliding clamp subunit) (DNA polymerase III subunit beta)

 DPO3B_CAUCN             Reviewed;         372 AA.
B8GXP6; P48198; Q45999;
16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
03-MAR-2009, sequence version 1.
22-NOV-2017, entry version 66.
RecName: Full=Beta sliding clamp;
Short=Beta clamp;
Short=Sliding clamp;
AltName: Full=Beta-clamp processivity factor;
AltName: Full=DNA polymerase III beta sliding clamp subunit;
AltName: Full=DNA polymerase III subunit beta;
Name=dnaN; OrderedLocusNames=CCNA_00155;
Caulobacter crescentus (strain NA1000 / CB15N).
Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
Caulobacteraceae; Caulobacter.
NCBI_TaxID=565050;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, AND DISRUPTION
PHENOTYPE.
STRAIN=NA1000 / CB15N;
PubMed=9079919; DOI=10.1128/jb.179.7.2319-2330.1997;
Roberts R.C., Shapiro L.;
"Transcription of genes encoding DNA replication proteins is
coincident with cell cycle control of DNA replication in Caulobacter
crescentus.";
J. Bacteriol. 179:2319-2330(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=NA1000 / CB15N;
PubMed=20472802; DOI=10.1128/JB.00255-10;
Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V.,
Walunas T.L., Crosson S.;
"The genetic basis of laboratory adaptation in Caulobacter
crescentus.";
J. Bacteriol. 192:3678-3688(2010).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 199-294.
PubMed=8226640; DOI=10.1128/jb.175.21.6970-6981.1993;
Rizzo M.F., Shapiro L., Gober J.;
"Asymmetric expression of the gyrase B gene from the replication-
competent chromosome in the Caulobacter crescentus predivisional
cell.";
J. Bacteriol. 175:6970-6981(1993).
[4]
FUNCTION, INTERACTION WITH SOCB AND HDAA, SUBCELLULAR LOCATION, AND
MUTAGENESIS OF GLY-179.
PubMed=24239291; DOI=10.1016/j.molcel.2013.10.014;
Aakre C.D., Phung T.N., Huang D., Laub M.T.;
"A bacterial toxin inhibits DNA replication elongation through a
direct interaction with the beta sliding clamp.";
Mol. Cell 52:617-628(2013).
-!- FUNCTION: Confers DNA tethering and processivity to DNA
polymerases and other proteins. Acts as a clamp, forming a ring
around DNA (a reaction catalyzed by the clamp-loading complex)
which diffuses in an ATP-independent manner freely and
bidirectionally along dsDNA. Initially characterized for its
ability to contact the catalytic subunit of DNA polymerase III
(Pol III), a complex, multichain enzyme responsible for most of
the replicative synthesis in bacteria; Pol III exhibits 3'-5'
exonuclease proofreading activity. The beta chain is required for
initiation of replication as well as for processivity of DNA
replication (By similarity). Interaction with toxin SocB via the
hydrophobic cleft blocks DNA replication elongation
(PubMed:24239291). {ECO:0000250|UniProtKB:P0A988,
ECO:0000269|PubMed:24239291}.
-!- SUBUNIT: Forms a ring-shaped head-to-tail homodimer around DNA
which binds and tethers DNA polymerases and other proteins to the
DNA. The DNA replisome complex has a single clamp-loading complex
(3 tau and 1 each of delta, delta', psi and chi subunits) which
binds 3 Pol III cores (1 core on the leading strand and 2 on the
lagging strand) each with a beta sliding clamp dimer. Additional
proteins in the replisome are other copies of gamma, psi and chi,
Ssb, DNA helicase and RNA primase (By similarity). Interacts with
DNA replication inhibitor toxin SocB (PubMed:24239291).
{ECO:0000250|UniProtKB:P0A988, ECO:0000269|PubMed:24239291}.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:24239291}.
Note=Forms a discrete locus during DNA replication that moves from
one pole to mid-cell before dispersing once DNA has been
replicated. Overexpressed tagged toxin SocB colocalizes with these
loci but causes their premature collapse (PubMed:24239291).
{ECO:0000269|PubMed:24239291}.
-!- INDUCTION: Has 3 promoters, 1 is heat inducible at 42 degrees
Celsius (probably under control of sigma-32). Transcription from
the other 2 promoters is induced just prior to the onset of DNA
replication, at the swarmer-to-stalked-cell transition, lasts
through the stalked-cell phase and then decreases in the
predivisional cell. {ECO:0000269|PubMed:9079919}.
-!- DISRUPTION PHENOTYPE: Essential, it cannot be deleted.
{ECO:0000269|PubMed:9079919}.
-!- SIMILARITY: Belongs to the beta sliding clamp family.
{ECO:0000305}.
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EMBL; U37793; AAB51448.1; -; Genomic_DNA.
EMBL; CP001340; ACL93622.1; -; Genomic_DNA.
EMBL; U00591; AAC43045.1; -; Genomic_DNA.
PIR; A49347; A49347.
RefSeq; WP_010918045.1; NC_011916.1.
RefSeq; YP_002515530.1; NC_011916.1.
ProteinModelPortal; B8GXP6; -.
SMR; B8GXP6; -.
EnsemblBacteria; ACL93622; ACL93622; CCNA_00155.
GeneID; 7332409; -.
KEGG; ccs:CCNA_00155; -.
PATRIC; fig|565050.3.peg.153; -.
HOGENOM; HOG000071791; -.
KO; K02338; -.
OMA; NYEAVIP; -.
OrthoDB; POG091H02B3; -.
PRO; PR:B8GXP6; -.
Proteomes; UP000001364; Chromosome.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0009360; C:DNA polymerase III complex; IEA:InterPro.
GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
CDD; cd00140; beta_clamp; 1.
InterPro; IPR001001; DNA_polIII_beta.
InterPro; IPR022635; DNA_polIII_beta_C.
InterPro; IPR022637; DNA_polIII_beta_cen.
InterPro; IPR022634; DNA_polIII_beta_N.
PANTHER; PTHR30478; PTHR30478; 1.
Pfam; PF00712; DNA_pol3_beta; 1.
Pfam; PF02767; DNA_pol3_beta_2; 1.
Pfam; PF02768; DNA_pol3_beta_3; 1.
PIRSF; PIRSF000804; DNA_pol_III_b; 1.
SMART; SM00480; POL3Bc; 1.
TIGRFAMs; TIGR00663; dnan; 1.
1: Evidence at protein level;
Complete proteome; Cytoplasm; DNA replication; DNA-binding;
DNA-directed DNA polymerase; Nucleotidyltransferase; Stress response;
Transferase.
CHAIN 1 372 Beta sliding clamp.
/FTId=PRO_0000378285.
MUTAGEN 179 179 G->C: Suppresses growth defects of
strains overexpressing SocB, no longer
interacts with SocB, still interacts with
HdaA. Replication forks do not collapse.
{ECO:0000269|PubMed:24239291}.
MUTAGEN 179 179 G->R: Suppresses growth defects of
strains overexpressing SocB, no longer
interacts with SocB or HdaA, cells are
filamentous.
{ECO:0000269|PubMed:24239291}.
CONFLICT 278 278 T -> N (in Ref. 3; AAC43045).
{ECO:0000305}.
CONFLICT 294 294 G -> D (in Ref. 3; AAC43045).
{ECO:0000305}.
SEQUENCE 372 AA; 40425 MW; B4B234778AD89BE4 CRC64;
MKLTIERAAL LKALGHVQSV VERRNTIPIL SNILLSAEGD RLSFSATDLD MEIIDEGFAQ
IDVPGQITAP AHTLYEIVRK LPDGADVSLS FSGDDPRLVI QAGRSRFNLP VLPAGDFPVM
SSDGLSSRIA VDTNELIRLI DKTRFAISTE ETRYYLNGLY VHTVNEGGET KLRAVATDGH
RLALAEMPAP EGAVGIPGVI VPRKTIAEAR RLMESAGETV DLQVSPQKVR FEFGAAALTS
KVIDGAFPDY MRVIPRDNAK ILTLDNDLFA KAVDRVATIS AEKSRSVKLA VEPGRITLTV
RNMEAGQAVE EVEVDYDGEP FEIGFNARYL LDVCGQIAGP QAEFRFADPA SPTLVVDPVD
PGVKYVLMPL RV


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