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Beta-agarase (EC 3.2.1.81)

 AGAR_PSEAF              Reviewed;         505 AA.
P13734;
01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
01-JAN-1990, sequence version 1.
05-JUL-2017, entry version 66.
RecName: Full=Beta-agarase;
EC=3.2.1.81;
Flags: Precursor;
Name=agrA;
Pseudoalteromonas atlantica (Alteromonas atlantica).
Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
Pseudoalteromonadaceae; Pseudoalteromonas.
NCBI_TaxID=288;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2914859; DOI=10.1128/jb.171.1.602-605.1989;
Belas R.;
"Sequence analysis of the agrA gene encoding beta-agarase from
Pseudomonas atlantica.";
J. Bacteriol. 171:602-605(1989).
-!- CATALYTIC ACTIVITY: Hydrolysis of (1->4)-beta-D-galactosidic
linkages in agarose, giving the tetramer as the predominant
product.
-!- SUBCELLULAR LOCATION: Secreted.
-!- SIMILARITY: Belongs to the glycosyl hydrolase 86 family.
{ECO:0000305}.
-!- WEB RESOURCE: Name=Worthington enzyme manual;
URL="http://www.worthington-biochem.com/AGAR/";
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EMBL; M22725; AAA25696.1; -; Genomic_DNA.
PIR; A32261; A32261.
ProteinModelPortal; P13734; -.
CAZy; GH86; Glycoside Hydrolase Family 86.
eggNOG; ENOG4105QRC; Bacteria.
eggNOG; ENOG4111JGH; LUCA.
GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
GO; GO:0033916; F:beta-agarase activity; IEA:UniProtKB-EC.
GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
InterPro; IPR017853; Glycoside_hydrolase_SF.
SUPFAM; SSF51445; SSF51445; 1.
3: Inferred from homology;
Glycosidase; Hydrolase; Secreted; Signal.
SIGNAL 1 23
CHAIN 24 505 Beta-agarase.
/FTId=PRO_0000012252.
ACT_SITE 200 200 Proton donor. {ECO:0000255}.
ACT_SITE 322 322 Nucleophile. {ECO:0000255}.
SEQUENCE 505 AA; 57617 MW; 66B75CBF486280B1 CRC64;
MLKVIPWLLV TSSLVAIPTY IHATTEVVVN LNVKHSVEGK SEFERKNHIK LHSTLNDNDW
QGEEDKLKYM MEELDVYFGR DNGGTVWNFN QAIEDPANIG YADPQNIIAR GQAQRETNWG
QNKSALHQYD GRGDLMIGGQ PRAHYLGNTS PCCGGSAWQA KGGDAVGDFL GQYVNEFFRS
AGDPVTKGHL APVYFEVLNE PLYQVTDAPH ELGLEQPIPP IDIFTFHNDV ADAFRQHNTH
IKIGGFTVAF PIFEQREFAR WEERMKLFID TSGSHMDVYS THFYDLEDDN RFKGSRLEAT
LDMIDQYSLL ALGETKPHVI SEYGGRNRPM ENAPWSALRD WWFLKTASPM LMQFLSRPDS
VLTSIPFVPI KALWGTAADG TPYNWRLLRQ QKEAPNETGE NWVFTEMVKF YQLWSDVKGT
RVDTFSTNSD FLIDSYVQND KAYVLISNLT EQAEKIVVHK YGAPASSQPT TRIKHLYLKG
AAPRLMKQVM RQISKKSRLL LKRLW


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