Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

CRISPR-associated endoribonuclease Cas13a (EC 3.1.-.-) (CRISPR-associated endoribonuclease C2c2) (EndoRNase) (LshC2c2)

 CS13A_LEPSD             Reviewed;        1389 AA.
P0DOC6;
05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
05-OCT-2016, sequence version 1.
22-NOV-2017, entry version 5.
RecName: Full=CRISPR-associated endoribonuclease Cas13a {ECO:0000303|PubMed:28111461};
EC=3.1.-.- {ECO:0000269|PubMed:27256883};
AltName: Full=CRISPR-associated endoribonuclease C2c2 {ECO:0000303|PubMed:27256883};
Short=EndoRNase;
AltName: Full=LshC2c2 {ECO:0000303|PubMed:26593719};
Name=cas13a {ECO:0000303|PubMed:28111461};
Synonyms=c2c2 {ECO:0000303|PubMed:26593719};
Leptotrichia shahii (strain DSM 19757 / CCUG 47503 / CIP 107916 / JCM
16776 / LB37).
Bacteria; Fusobacteria; Fusobacteriales; Leptotrichiaceae;
Leptotrichia.
NCBI_TaxID=1122172;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=DSM 19757 / CCUG 47503 / CIP 107916 / JCM 16776 / LB37;
Kyrpides N., Huntemann M., Han J., Chen A., Mavromatis K.,
Markowitz V., Palaniappan K., Ivanova N., Schaumberg A., Pati A.,
Liolios K., Nordberg H.P., Cantor M.N., Hua S.X., Woyke T.;
Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
[2]
IDENTIFICATION, AND DOMAIN.
PubMed=26593719; DOI=10.1016/j.molcel.2015.10.008;
Shmakov S., Abudayyeh O.O., Makarova K.S., Wolf Y.I., Gootenberg J.S.,
Semenova E., Minakhin L., Joung J., Konermann S., Severinov K.,
Zhang F., Koonin E.V.;
"Discovery and functional characterization of diverse class 2 CRISPR-
Cas systems.";
Mol. Cell 60:385-397(2015).
[3]
FUNCTION IN RNA PHAGE RESISTANCE, FUNCTION AS AN SS-RNASE, ACTIVE
SITE, COFACTOR, ENZYME REGULATION, SUBUNIT, DOMAIN, BIOTECHNOLOGY,
MUTAGENESIS OF ARG-597; HIS-602; ARG-1278 AND HIS-1283, AND
RNA-BINDING.
STRAIN=DSM 19757 / CCUG 47503 / CIP 107916 / JCM 16776 / LB37;
PubMed=27256883; DOI=10.1126/science.aaf5573;
Abudayyeh O.O., Gootenberg J.S., Konermann S., Joung J.,
Slaymaker I.M., Cox D.B., Shmakov S., Makarova K.S., Semenova E.,
Minakhin L., Severinov K., Regev A., Lander E.S., Koonin E.V.,
Zhang F.;
"C2c2 is a single-component programmable RNA-guided RNA-targeting
CRISPR effector.";
Science 0:0-0(2016).
[4]
FUNCTION IN CRRNA PROCESSING, AND FUNCTION AS AN ENDORIBONUCLEASE.
STRAIN=DSM 19757 / CCUG 47503 / CIP 107916 / JCM 16776 / LB37;
PubMed=27669025; DOI=10.1038/nature19802;
East-Seletsky A., O'Connell M.R., Knight S.C., Burstein D., Cate J.H.,
Tjian R., Doudna J.A.;
"Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA
processing and RNA detection.";
Nature 538:270-273(2016).
[5]
FUNCTION IN CRRNA PROCESSING, AND FUNCTION AS AN ENDORIBONUCLEASE.
PubMed=28475872; DOI=10.1016/j.molcel.2017.04.008;
East-Seletsky A., O'Connell M.R., Burstein D., Knott G.J.,
Doudna J.A.;
"RNA targeting by functionally orthogonal type VI-A CRISPR-Cas
enzymes.";
Mol. Cell 66:373-383(2017).
[6]
NOMENCLATURE.
PubMed=28111461; DOI=10.1038/nrmicro.2016.184;
Shmakov S., Smargon A., Scott D., Cox D., Pyzocha N., Yan W.,
Abudayyeh O.O., Gootenberg J.S., Makarova K.S., Wolf Y.I.,
Severinov K., Zhang F., Koonin E.V.;
"Diversity and evolution of class 2 CRISPR-Cas systems.";
Nat. Rev. Microbiol. 15:169-182(2017).
[7] {ECO:0000244|PDB:5WTJ, ECO:0000244|PDB:5WTK}
X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) WITH AND WITHOUT CRRNA,
FUNCTION IN CRRNA PROCESSING, FUNCTION IN TARGET SSRNA CLEAVAGE,
FUNCTION AS AN ENDORIBONUCLEASE, ACTIVE SITE, DOMAIN, MUTAGENESIS OF
TYR-330; TYR-334; ARG-438; LYS-441; LYS-471; HIS-509; ASN-598;
ARG-858; TRP-865; ASN-1279; ARG-1312; ASN-1315 AND SER-1316, AND
RNA-BINDING.
PubMed=28086085; DOI=10.1016/j.cell.2016.12.031;
Liu L., Li X., Wang J., Wang M., Chen P., Yin M., Li J., Sheng G.,
Wang Y.;
"Two distant catalytic sites are responsible for C2c2 RNase
activities.";
Cell 168:121-134(2017).
-!- FUNCTION: CRISPR (clustered regularly interspaced short
palindromic repeat), is an adaptive immune system that provides
protection against mobile genetic elements (viruses, transposable
elements and conjugative plasmids). CRISPR clusters contain
sequences complementary to antecedent mobile elements (spacers)
and target invading nucleic acids. Unlike many single-component
effectors, this CRISPR-Cas system targets RNA (PubMed:27256883,
PubMed:28086085). CRISPR clusters are transcribed from pre-CRISPR
RNA (crRNA) and processed into crRNA (optimally 28 nucleotides in
this system) by this protein (PubMed:27256883, PubMed:27669025,
PubMed:28475872, PubMed:28086085). This protein processes pre-
crRNA at a 'non-typical' site 1 nucleotide upstream of the pre-
crRNA stem-loop; it cleaves pre-crRNA from L.buccalis and L.wadei
in a similar fashion, whereas the enzymes from the latter 2
bacteria cleave their own pre-crRNA 3 nt further upstream
(PubMed:28475872). When the appropriate target sequences are
cloned into the CRISPR array, confers immunity to ssRNA(+)
enterobacteria phage MS2 (PubMed:27256883). Cleaves linear target
ssRNA in a crRNA-dependent fashion, preferentially before U
residues; has no activity on partially dsRNA, ssDNA or dsDNA
(PubMed:27256883). RNA secondary structure surrounding the target
influence the cleavage site and efficiency; unlike other CRISPR-
Cas effectors Cas13a cleaves outside of the crRNA binding site
(PubMed:27256883). In the presence of a viable RNA target other
RNAs are also degraded (called collateral RNA degradation),
suggesting this type of CRISPR-Cas might also prevent viral spread
by inducing programmed cell death or dormancy (PubMed:27256883).
This system has a 3' protospacer flanking site (PFS), it does not
cleave when the 3' PFS is G (PFS is equivalent to PAM, the
protospacer adjacent motif) (PubMed:27256883). Mutations of its
active site residues results in an RNA-programmed RNA-binding
protein (PubMed:27256883). {ECO:0000269|PubMed:27256883,
ECO:0000269|PubMed:27669025, ECO:0000269|PubMed:28086085,
ECO:0000269|PubMed:28475872}.
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Evidence={ECO:0000269|PubMed:27256883};
Note=ssRNA target cleavage requires Mg(2+), weak target cleavage
is seen with Mn(2+) and Ca(2+). {ECO:0000269|PubMed:27256883};
-!- ENZYME REGULATION: RNase activity on target is decreased by EDTA
(PubMed:27256883). Target RNA acts as an activator for non-
specific ssRNA degradation (PubMed:27256883).
{ECO:0000269|PubMed:27256883}.
-!- SUBUNIT: Monomer (PubMed:27256883). {ECO:0000305|PubMed:27256883}.
-!- DOMAIN: The target ssRNase active sites are within the 2 HEPN-like
folds which interact in vivo (PubMed:26593719, PubMed:27256883,
PubMed:28086085). The X-ray structure in complex with crRNA shows
a crRNA-recognition lobe (REC, residues 1-498) which makes most of
the contacts with crRNA and is essential for pre-crRNA cleavage,
and a nuclease lobe (NUC, residues 499-1389) which also makes a
few contacts with the crRNA repeat and is responsible for target
ssRNA cleavage (PubMed:28086085). Binding of crRNA induces
conformational changes that probably stabilize crRNA-binding and
facilitate target ssRNA recognition (PubMed:28086085). Mutagenesis
of the crRNA and this protein show the stem loop and 3'-direct
repeat of the crRNA are essential for target ssRNase while the 5'-
end of the crRNA and its bulge-containing stem are essential for
pre-crRNA cleavage (PubMed:28086085).
{ECO:0000269|PubMed:28086085, ECO:0000305|PubMed:26593719,
ECO:0000305|PubMed:27256883}.
-!- BIOTECHNOLOGY: Can be reprogrammed to target specific mRNAs in an
E.coli system, which might be useful to create specific RNA-
targeting tools (PubMed:27256883). {ECO:0000269|PubMed:27256883}.
-!- MISCELLANEOUS: Part of a type VI-A uridine-prefering CRISPR-Cas
system. {ECO:0000305|PubMed:26593719, ECO:0000305|PubMed:28111461,
ECO:0000305|PubMed:28475872}.
-!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease
Cas13a family. {ECO:0000305|PubMed:28111461}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; ARDD01000012; -; NOT_ANNOTATED_CDS; Genomic_DNA.
RefSeq; WP_018451595.1; NZ_KB890278.1.
PDB; 5WTJ; X-ray; 3.50 A; A/B=1-1389.
PDB; 5WTK; X-ray; 2.65 A; A=1-1389.
PDBsum; 5WTJ; -.
PDBsum; 5WTK; -.
SMR; P0DOC6; -.
GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
1: Evidence at protein level;
3D-structure; Antiviral defense; Coiled coil; Endonuclease; Hydrolase;
Magnesium; Nuclease; Repeat; RNA-binding.
CHAIN 1 1389 CRISPR-associated endoribonuclease
Cas13a.
/FTId=PRO_0000437503.
REGION 1 347 NTD. {ECO:0000305|PubMed:28086085}.
REGION 330 342 Binds crRNA.
{ECO:0000269|PubMed:28086085}.
REGION 348 498 Helical-1. {ECO:0000305|PubMed:26593719,
ECO:0000305|PubMed:27256883,
ECO:0000305|PubMed:28086085}.
REGION 405 408 Binds crRNA.
{ECO:0000269|PubMed:28086085}.
REGION 432 436 Binds crRNA.
{ECO:0000269|PubMed:28086085}.
REGION 471 475 Binds crRNA.
{ECO:0000269|PubMed:28086085}.
REGION 499 636 HEPN-like fold 1-I.
{ECO:0000305|PubMed:26593719,
ECO:0000305|PubMed:27256883,
ECO:0000305|PubMed:28086085}.
REGION 502 509 Binds crRNA.
{ECO:0000269|PubMed:28086085}.
REGION 637 828 Helical-2. {ECO:0000305|PubMed:26593719,
ECO:0000305|PubMed:27256883,
ECO:0000305|PubMed:28086085}.
REGION 829 899 HEPN-like fold 1-II.
{ECO:0000305|PubMed:28086085}.
REGION 853 858 Binds crRNA.
{ECO:0000269|PubMed:28086085}.
REGION 900 1170 Linker. {ECO:0000305|PubMed:26593719,
ECO:0000305|PubMed:27256883,
ECO:0000305|PubMed:28086085}.
REGION 1170 1290 HEPN-like fold 2.
{ECO:0000305|PubMed:26593719,
ECO:0000305|PubMed:27256883,
ECO:0000305|PubMed:28086085}.
REGION 1311 1316 Binds crRNA.
{ECO:0000269|PubMed:28086085}.
REGION 1338 1339 Binds crRNA.
{ECO:0000269|PubMed:28086085}.
COILED 893 920 {ECO:0000255}.
COILED 968 1046 {ECO:0000255}.
COILED 1101 1131 {ECO:0000255}.
COMPBIAS 269 477 Lys-rich. {ECO:0000255|PROSITE-
ProRule:PRU00012}.
COMPBIAS 906 1164 Lys-rich. {ECO:0000255|PROSITE-
ProRule:PRU00012}.
ACT_SITE 438 438 For pre-crRNA processing.
{ECO:0000305|PubMed:28086085}.
ACT_SITE 441 441 For pre-crRNA processing.
{ECO:0000305|PubMed:28086085}.
ACT_SITE 597 597 For target ssRNA cleavage.
{ECO:0000305|PubMed:27256883}.
ACT_SITE 602 602 For target ssRNA cleavage.
{ECO:0000305|PubMed:27256883}.
ACT_SITE 1278 1278 For target ssRNA cleavage.
{ECO:0000305|PubMed:27256883}.
ACT_SITE 1283 1283 For target ssRNA cleavage.
{ECO:0000305|PubMed:27256883}.
BINDING 219 219 crRNA. {ECO:0000269|PubMed:28086085}.
BINDING 441 441 crRNA. {ECO:0000269|PubMed:28086085}.
BINDING 489 489 crRNA. {ECO:0000269|PubMed:28086085}.
BINDING 759 759 crRNA. {ECO:0000269|PubMed:28086085}.
BINDING 865 865 crRNA. {ECO:0000269|PubMed:28086085}.
MUTAGEN 330 330 Y->A: Wild-type cleavage of target RNA
and pre-crRNA processing.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 334 334 Y->A: No cleavage of target RNA, no
change in pre-crRNA processing.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 438 438 R->A: No pre-crRNA processing.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 441 441 K->A: No pre-crRNA processing.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 471 471 K->A: Severely impairs pre-crRNA
processing.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 509 509 H->A: No cleavage of target ssRNA, no
change in pre-crRNA processing.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 597 597 R->A: No longer protects against
infection by MS2 phage, loss of ssRNase
activity. {ECO:0000269|PubMed:27256883}.
MUTAGEN 598 598 N->A: No cleavage of target RNA.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 602 602 H->A: No longer protects against
infection by MS2 phage, loss of ssRNase
activity. {ECO:0000269|PubMed:27256883}.
MUTAGEN 858 858 R->A: No cleavage of target RNA, no
change in pre-crRNA processing.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 865 865 W->A: No cleavage of target RNA,
decreased pre-crRNA processing.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 1278 1278 R->A: No longer protects against
infection by MS2 phage, loss of ssRNase
activity, still binds both crRNA and
target ssRNA.
{ECO:0000269|PubMed:27256883}.
MUTAGEN 1279 1279 N->A: No cleavage of target RNA.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 1283 1283 H->A: No longer protects against
infection by MS2 phage, loss of ssRNase
activity. {ECO:0000269|PubMed:27256883}.
MUTAGEN 1312 1312 R->A: No cleavage of target RNA, no
change in pre-crRNA processing.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 1315 1315 N->A: Wild-type cleavage of target RNA,
decreased pre-crRNA processing.
{ECO:0000269|PubMed:28086085}.
MUTAGEN 1316 1316 S->A: Wild-type cleavage of target RNA
and pre-crRNA processing.
{ECO:0000269|PubMed:28086085}.
STRAND 4 8 {ECO:0000244|PDB:5WTK}.
STRAND 11 14 {ECO:0000244|PDB:5WTK}.
STRAND 16 25 {ECO:0000244|PDB:5WTK}.
STRAND 27 31 {ECO:0000244|PDB:5WTK}.
STRAND 33 40 {ECO:0000244|PDB:5WTK}.
STRAND 42 44 {ECO:0000244|PDB:5WTK}.
HELIX 51 63 {ECO:0000244|PDB:5WTK}.
HELIX 67 74 {ECO:0000244|PDB:5WTK}.
TURN 77 79 {ECO:0000244|PDB:5WTK}.
STRAND 80 82 {ECO:0000244|PDB:5WTK}.
HELIX 184 208 {ECO:0000244|PDB:5WTK}.
HELIX 221 230 {ECO:0000244|PDB:5WTK}.
HELIX 235 250 {ECO:0000244|PDB:5WTK}.
HELIX 254 262 {ECO:0000244|PDB:5WTK}.
STRAND 267 269 {ECO:0000244|PDB:5WTK}.
HELIX 273 282 {ECO:0000244|PDB:5WTK}.
STRAND 285 287 {ECO:0000244|PDB:5WTK}.
HELIX 291 304 {ECO:0000244|PDB:5WTK}.
HELIX 320 334 {ECO:0000244|PDB:5WTK}.
HELIX 339 345 {ECO:0000244|PDB:5WTK}.
HELIX 350 364 {ECO:0000244|PDB:5WTK}.
HELIX 367 377 {ECO:0000244|PDB:5WTK}.
HELIX 380 393 {ECO:0000244|PDB:5WTK}.
HELIX 399 411 {ECO:0000244|PDB:5WTK}.
STRAND 415 417 {ECO:0000244|PDB:5WTK}.
HELIX 422 450 {ECO:0000244|PDB:5WTK}.
HELIX 460 463 {ECO:0000244|PDB:5WTK}.
HELIX 465 493 {ECO:0000244|PDB:5WTK}.
HELIX 502 532 {ECO:0000244|PDB:5WTK}.
STRAND 533 539 {ECO:0000244|PDB:5WTK}.
STRAND 555 557 {ECO:0000244|PDB:5WTK}.
HELIX 564 571 {ECO:0000244|PDB:5WTK}.
HELIX 577 579 {ECO:0000244|PDB:5WTK}.
HELIX 583 587 {ECO:0000244|PDB:5WTK}.
HELIX 590 601 {ECO:0000244|PDB:5WTK}.
STRAND 611 613 {ECO:0000244|PDB:5WTK}.
HELIX 616 629 {ECO:0000244|PDB:5WTK}.
TURN 633 635 {ECO:0000244|PDB:5WTK}.
TURN 638 644 {ECO:0000244|PDB:5WTK}.
HELIX 648 655 {ECO:0000244|PDB:5WTK}.
TURN 664 666 {ECO:0000244|PDB:5WTK}.
HELIX 667 669 {ECO:0000244|PDB:5WTK}.
HELIX 673 686 {ECO:0000244|PDB:5WTK}.
STRAND 687 689 {ECO:0000244|PDB:5WTK}.
TURN 696 698 {ECO:0000244|PDB:5WTK}.
HELIX 699 719 {ECO:0000244|PDB:5WTK}.
HELIX 732 740 {ECO:0000244|PDB:5WTK}.
HELIX 750 761 {ECO:0000244|PDB:5WTK}.
TURN 762 765 {ECO:0000244|PDB:5WTK}.
HELIX 769 788 {ECO:0000244|PDB:5WTK}.
HELIX 791 793 {ECO:0000244|PDB:5WTK}.
HELIX 803 811 {ECO:0000244|PDB:5WTK}.
HELIX 835 844 {ECO:0000244|PDB:5WTK}.
HELIX 849 866 {ECO:0000244|PDB:5WTK}.
HELIX 872 894 {ECO:0000244|PDB:5WTK}.
HELIX 902 912 {ECO:0000244|PDB:5WTK}.
STRAND 1008 1011 {ECO:0000244|PDB:5WTK}.
HELIX 1012 1014 {ECO:0000244|PDB:5WTK}.
HELIX 1018 1032 {ECO:0000244|PDB:5WTK}.
STRAND 1041 1044 {ECO:0000244|PDB:5WTK}.
TURN 1045 1047 {ECO:0000244|PDB:5WTK}.
TURN 1050 1052 {ECO:0000244|PDB:5WTK}.
HELIX 1053 1055 {ECO:0000244|PDB:5WTK}.
HELIX 1061 1064 {ECO:0000244|PDB:5WTK}.
HELIX 1070 1076 {ECO:0000244|PDB:5WTK}.
TURN 1077 1079 {ECO:0000244|PDB:5WTK}.
HELIX 1080 1085 {ECO:0000244|PDB:5WTK}.
TURN 1089 1091 {ECO:0000244|PDB:5WTK}.
HELIX 1094 1102 {ECO:0000244|PDB:5WTK}.
HELIX 1104 1118 {ECO:0000244|PDB:5WTK}.
HELIX 1123 1135 {ECO:0000244|PDB:5WTK}.
HELIX 1137 1141 {ECO:0000244|PDB:5WTK}.
STRAND 1143 1148 {ECO:0000244|PDB:5WTK}.
HELIX 1149 1169 {ECO:0000244|PDB:5WTK}.
HELIX 1172 1208 {ECO:0000244|PDB:5WTK}.
TURN 1209 1211 {ECO:0000244|PDB:5WTK}.
STRAND 1214 1217 {ECO:0000244|PDB:5WTK}.
STRAND 1231 1233 {ECO:0000244|PDB:5WTK}.
HELIX 1234 1237 {ECO:0000244|PDB:5WTK}.
STRAND 1238 1245 {ECO:0000244|PDB:5WTK}.
HELIX 1246 1258 {ECO:0000244|PDB:5WTK}.
STRAND 1264 1271 {ECO:0000244|PDB:5WTK}.
TURN 1272 1275 {ECO:0000244|PDB:5WTK}.
HELIX 1277 1282 {ECO:0000244|PDB:5WTK}.
TURN 1283 1287 {ECO:0000244|PDB:5WTK}.
STRAND 1292 1294 {ECO:0000244|PDB:5WTK}.
HELIX 1296 1306 {ECO:0000244|PDB:5WTK}.
TURN 1307 1309 {ECO:0000244|PDB:5WTK}.
HELIX 1311 1313 {ECO:0000244|PDB:5WTK}.
HELIX 1316 1322 {ECO:0000244|PDB:5WTK}.
TURN 1326 1328 {ECO:0000244|PDB:5WTK}.
HELIX 1333 1337 {ECO:0000244|PDB:5WTK}.
STRAND 1342 1345 {ECO:0000244|PDB:5WTK}.
HELIX 1347 1349 {ECO:0000244|PDB:5WTK}.
TURN 1359 1362 {ECO:0000244|PDB:5WTK}.
HELIX 1369 1380 {ECO:0000244|PDB:5WTK}.
SEQUENCE 1389 AA; 166277 MW; 20A892EA93E12E9F CRC64;
MGNLFGHKRW YEVRDKKDFK IKRKVKVKRN YDGNKYILNI NENNNKEKID NNKFIRKYIN
YKKNDNILKE FTRKFHAGNI LFKLKGKEGI IRIENNDDFL ETEEVVLYIE AYGKSEKLKA
LGITKKKIID EAIRQGITKD DKKIEIKRQE NEEEIEIDIR DEYTNKTLND CSIILRIIEN
DELETKKSIY EIFKNINMSL YKIIEKIIEN ETEKVFENRY YEEHLREKLL KDDKIDVILT
NFMEIREKIK SNLEILGFVK FYLNVGGDKK KSKNKKMLVE KILNINVDLT VEDIADFVIK
ELEFWNITKR IEKVKKVNNE FLEKRRNRTY IKSYVLLDKH EKFKIERENK KDKIVKFFVE
NIKNNSIKEK IEKILAEFKI DELIKKLEKE LKKGNCDTEI FGIFKKHYKV NFDSKKFSKK
SDEEKELYKI IYRYLKGRIE KILVNEQKVR LKKMEKIEIE KILNESILSE KILKRVKQYT
LEHIMYLGKL RHNDIDMTTV NTDDFSRLHA KEELDLELIT FFASTNMELN KIFSRENINN
DENIDFFGGD REKNYVLDKK ILNSKIKIIR DLDFIDNKNN ITNNFIRKFT KIGTNERNRI
LHAISKERDL QGTQDDYNKV INIIQNLKIS DEEVSKALNL DVVFKDKKNI ITKINDIKIS
EENNNDIKYL PSFSKVLPEI LNLYRNNPKN EPFDTIETEK IVLNALIYVN KELYKKLILE
DDLEENESKN IFLQELKKTL GNIDEIDENI IENYYKNAQI SASKGNNKAI KKYQKKVIEC
YIGYLRKNYE ELFDFSDFKM NIQEIKKQIK DINDNKTYER ITVKTSDKTI VINDDFEYII
SIFALLNSNA VINKIRNRFF ATSVWLNTSE YQNIIDILDE IMQLNTLRNE CITENWNLNL
EEFIQKMKEI EKDFDDFKIQ TKKEIFNNYY EDIKNNILTE FKDDINGCDV LEKKLEKIVI
FDDETKFEID KKSNILQDEQ RKLSNINKKD LKKKVDQYIK DKDQEIKSKI LCRIIFNSDF
LKKYKKEIDN LIEDMESENE NKFQEIYYPK ERKNELYIYK KNLFLNIGNP NFDKIYGLIS
NDIKMADAKF LFNIDGKNIR KNKISEIDAI LKNLNDKLNG YSKEYKEKYI KKLKENDDFF
AKNIQNKNYK SFEKDYNRVS EYKKIRDLVE FNYLNKIESY LIDINWKLAI QMARFERDMH
YIVNGLRELG IIKLSGYNTG ISRAYPKRNG SDGFYTTTAY YKFFDEESYK KFEKICYGFG
IDLSENSEIN KPENESIRNY ISHFYIVRNP FADYSIAEQI DRVSNLLSYS TRYNNSTYAS
VFEVFKKDVN LDYDELKKKF KLIGNNDILE RLMKPKKVSV LELESYNSDY IKNLIIELLT
KIENTNDTL


Related products :

Catalog number Product name Quantity
EIAAB12950 Bos taurus,Bovine,ENDOU,Poly(U)-specific endoribonuclease,Protein endoU,Uridylate-specific endoribonuclease
EIAAB12952 ENDOU,Homo sapiens,Human,Placental protein 11,Poly(U)-specific endoribonuclease,PP11,Protein endoU,Uridylate-specific endoribonuclease
A-9000-050 CRISPR per Cas9 Monoclonal Antibody 50
A-9000-010 CRISPR per Cas9 Monoclonal Antibody 10
A-1111-010 CRISPR per Cas9 Polyclonal Antibody 10
A-1111-050 CRISPR per Cas9 Polyclonal Antibody 50
A-9000-100 CRISPR per Cas9 Monoclonal Antibody 100
A-1111-100 CRISPR per Cas9 Polyclonal Antibody 100
ASK-7700 CRISPR Mouse per Rat Embryo Injection-Ready Rosa26 Kit 1 Kit
S303 CRISPR.Cas9 and TALEN vectors validation services genomic DNA sample
S303 CRISPR per Cas9 and TALEN vectors validation services sample
ASK-7701 CRISPR Transfection-Ready Rosa26 Knock-in Kit for Mouse Cell Line 1 Kit
AP11794PU-N Endoribonuclease dcr_1 (Center) 0.1 mg
AP11793PU-N Endoribonuclease dcr_1 (C_term) 0.1 mg
AP11794PU-N Endoribonuclease dcr_1 (Center) 0.1 mg
EH731 Endoribonuclease Dicer Elisa Kit 96T
AP11793PU-N Endoribonuclease dcr_1 (C_term) 0.1 mg
'AP11794CP-N Endoribonuclease dcr-1 Control Peptide antibody Ab CP 0.1 mg
'AP11794CP-N Endoribonuclease dcr-1 Control Peptide antibody CP 0.1 mg
'AP11793CP-N Endoribonuclease dcr-1 Control Peptide antibody Ab CP 0.1 mg
'AP11794CP-N Endoribonuclease dcr-1 Control Peptide antibody _CP 0.1 mg
'AP11793CP-N Endoribonuclease dcr-1 Control Peptide antibody _CP 0.1 mg
'AP11793CP-N Endoribonuclease dcr-1 Control Peptide antibody CP 0.1 mg
AP11794CP-N Endoribonuclease dcr_1 Control Peptide 0.1 mg
AP11793CP-N Endoribonuclease dcr_1 Control Peptide 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur