Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

CRISPR-associated exonuclease Csa1

 CSA1_SULSO              Reviewed;         291 AA.
Q97YD4;
01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
01-OCT-2001, sequence version 1.
23-MAY-2018, entry version 71.
RecName: Full=CRISPR-associated exonuclease Csa1;
Name=csa1; OrderedLocusNames=SSO1391;
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 /
P2).
Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
Sulfolobus.
NCBI_TaxID=273057;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
PubMed=11427726; DOI=10.1073/pnas.141222098;
She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G.,
Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A.,
De Moors A., Erauso G., Fletcher C., Gordon P.M.K.,
Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X.,
Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N.,
Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
"The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
[2]
POSSIBLE COFACTOR.
STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
PubMed=23056615; DOI=10.1371/journal.pone.0047232;
Zhang J., Kasciukovic T., White M.F.;
"The CRISPR associated protein Cas4 is a 5' to 3' DNA exonuclease with
an iron-sulfur cluster.";
PLoS ONE 7:E47232-E47232(2012).
[3]
FUNCTION, COFACTOR, AND MUTAGENESIS OF GLU-195.
PubMed=24171432; DOI=10.1021/ja408729b;
Lemak S., Beloglazova N., Nocek B., Skarina T., Flick R., Brown G.,
Popovic A., Joachimiak A., Savchenko A., Yakunin A.F.;
"Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S]
cluster containing Cas4 nuclease SSO0001 from Sulfolobus
solfataricus.";
J. Am. Chem. Soc. 135:17476-17487(2013).
[4]
FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT, AND
MUTAGENESIS OF CYS-52; TYR-59; HIS-80; ASP-184; GLU-195; LYS-197;
TYR-201; TYR-212; GLU-217; CYS-230; CYS-243; ARG-260; CYS-283 AND
CYS-289.
PubMed=25200083; DOI=10.1093/nar/gku797;
Lemak S., Nocek B., Beloglazova N., Skarina T., Flick R., Brown G.,
Joachimiak A., Savchenko A., Yakunin A.F.;
"The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum
calidifontis contains a [2Fe-2S] cluster: crystal structure and
nuclease activity.";
Nucleic Acids Res. 42:11144-11155(2014).
-!- FUNCTION: CRISPR (clustered regularly interspaced short
palindromic repeat) is an adaptive immune system that provides
protection against mobile genetic elements (viruses, transposable
elements and conjugative plasmids). CRISPR clusters contain
sequences complementary to antecedent mobile elements and target
invading nucleic acids. CRISPR clusters are transcribed and
processed into CRISPR RNA (crRNA) (By similarity). A ssDNA
exonuclease that has both 5' to 3' and 3' to 5' activity, yielding
5'-OH and 3'-phosphate groups. Has Mn(2+)-dependent endonuclease
activity on circular ssDNA. Can unwind dsDNA; unwinding does not
require ATP. {ECO:0000250, ECO:0000269|PubMed:24171432,
ECO:0000269|PubMed:25200083}.
-!- COFACTOR:
Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
Evidence={ECO:0000269|PubMed:24171432,
ECO:0000269|PubMed:25200083};
Name=Co(2+); Xref=ChEBI:CHEBI:48828;
Evidence={ECO:0000269|PubMed:24171432,
ECO:0000269|PubMed:25200083};
Note=Mn(2+) required for nuclease activity. Can also utilize
Co(2+) and to a lesser extent Mg(2+) or Ni(2+).
{ECO:0000269|PubMed:24171432, ECO:0000269|PubMed:25200083};
-!- COFACTOR:
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
Evidence={ECO:0000250|UniProtKB:Q97TX9,
ECO:0000303|PubMed:23056615, ECO:0000303|PubMed:24171432,
ECO:0000303|PubMed:25200083};
Note=Binds 1 [4Fe-4S] cluster per subunit. Not required for
nuclease activity, since mutation of the Cys residues leads to a
colorless but active protein. {ECO:0000250|UniProtKB:Q97TX9,
ECO:0000303|PubMed:23056615, ECO:0000303|PubMed:24171432,
ECO:0000303|PubMed:25200083};
-!- BIOPHYSICOCHEMICAL PROPERTIES:
pH dependence:
Optimum pH is 7-9. {ECO:0000269|PubMed:25200083};
-!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:25200083}.
-!- SIMILARITY: Belongs to the CRISPR-associated protein Csa1 family.
{ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; AE006641; AAK41627.1; -; Genomic_DNA.
PIR; D90296; D90296.
ProteinModelPortal; Q97YD4; -.
STRING; 273057.SSO1391; -.
PRIDE; Q97YD4; -.
EnsemblBacteria; AAK41627; AAK41627; SSO1391.
KEGG; sso:SSO1391; -.
PATRIC; fig|273057.12.peg.1407; -.
eggNOG; arCOG04195; Archaea.
eggNOG; COG4343; LUCA.
HOGENOM; HOG000245955; -.
InParanoid; Q97YD4; -.
KO; K19085; -.
OMA; GYLCYVN; -.
OrthoDB; POG093Z07RM; -.
Proteomes; UP000001974; Chromosome.
GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0008310; F:single-stranded DNA 3'-5' exodeoxyribonuclease activity; IDA:UniProtKB.
GO; GO:0045145; F:single-stranded DNA 5'-3' exodeoxyribonuclease activity; IDA:UniProtKB.
GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IDA:UniProtKB.
GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
GO; GO:0032508; P:DNA duplex unwinding; IDA:UniProtKB.
Gene3D; 3.90.320.10; -; 1.
InterPro; IPR009260; CRISPR-ass_Csa1.
InterPro; IPR011604; Exonuc_phg/RecB_C.
Pfam; PF06023; Csa1; 1.
PIRSF; PIRSF009226; UCP009226; 1.
TIGRFAMs; TIGR01896; cas_AF1879; 1.
1: Evidence at protein level;
4Fe-4S; Antiviral defense; Complete proteome; Exonuclease; Hydrolase;
Iron; Iron-sulfur; Manganese; Metal-binding; Nuclease;
Reference proteome.
CHAIN 1 291 CRISPR-associated exonuclease Csa1.
/FTId=PRO_0000422231.
METAL 52 52 Iron-sulfur (4Fe-4S).
{ECO:0000250|UniProtKB:Q97TX9}.
METAL 279 279 Iron-sulfur (4Fe-4S).
{ECO:0000250|UniProtKB:Q97TX9}.
METAL 283 283 Iron-sulfur (4Fe-4S).
{ECO:0000250|UniProtKB:Q97TX9}.
METAL 289 289 Iron-sulfur (4Fe-4S).
{ECO:0000250|UniProtKB:Q97TX9}.
MUTAGEN 52 52 C->A: Nearly wild-type protein.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 59 59 Y->A: No effect.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 80 80 H->A: No exonuclease, endonuclease, or
DNA unwinding activity.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 184 184 D->A: No exonuclease, endonuclease, or
DNA unwinding activity.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 195 195 E->A: No exonuclease, endonuclease, or
DNA unwinding activity.
{ECO:0000269|PubMed:24171432,
ECO:0000269|PubMed:25200083}.
MUTAGEN 197 197 K->A: No exonuclease, endonuclease, or
DNA unwinding activity.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 201 201 Y->A: No effect.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 212 212 Y->A: No exonuclease, endonuclease, or
DNA unwinding activity.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 217 217 E->A: Reduced exonuclease and DNA
unwinding, no endonuclease activity.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 230 230 C->A: No effect.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 243 243 C->A: No effect.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 260 260 R->A: Wild-type exonuclease, reduced DNA
unwinding, no endonuclease activity.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 283 283 C->A: Wild-type exonuclease and DNA
unwinding, reduced endonuclease activity.
{ECO:0000269|PubMed:25200083}.
MUTAGEN 289 289 C->A: Wild-type exonuclease and DNA
unwinding, reduced endonuclease activity.
{ECO:0000269|PubMed:25200083}.
SEQUENCE 291 AA; 33491 MW; BE68EC7DC4F5AC0B CRC64;
MFFTHSDMLL LSKRIKKLPK NVDEELRGWN WSEPPVYTRS LSQVSISEMV YCSTLRNVYL
KVKGFRGEIG RQILQGSLIH TIYAIGIEAI KRFIYSRESI DGSTLRTLMG DEFYSLLKDL
REEEGIYAKV LWDHITNIYS AELDRVRSKF TNLTRDSLVS QVVPFYVEFP VDGSLLGLTN
LRVDAFIPHL PLIAEMKTGK YRYTHELSLA GYALAIESQY EIPIDFGYLC YVTVTEKEVK
NNCKLIPISD SLRSEFLDMR DKAQDIMDKG VDPGIAKDCE SDCMFYKVCH P


Related products :

Catalog number Product name Quantity
EIAAB13447 EXO1,EXOI,Exonuclease 1,Exonuclease I,HEX1,hExo1,hExoI,Homo sapiens,Human
18-003-43366 RNA exonuclease 4 - EC 3.1.-.-; Exonuclease XPMC2; hPMC2; Prevents mitotic catastrophe 2 protein homolog Polyclonal 0.1 mg Protein A
18-003-43097 RNA exonuclease 4 - EC 3.1.-.-; Exonuclease XPMC2; hPMC2; Prevents mitotic catastrophe 2 protein homolog Polyclonal 0.05 mg Aff Pur
EIAAB13448 Exo1,Exonuclease 1,Exonuclease I,mExo1,Mouse,Mus musculus
EIAAB25855 EXD3,Exonuclease 3'-5' domain-containing protein 3,HBE269,Homo sapiens,Human,Probable exonuclease mut-7 homolog
29-286 ISG20 belongs to the the exonuclease superfamily. It is exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. It degrades RNA at a rate that is approximately 35-fold hi 0.05 mg
29-287 ISG20 belongs to the the exonuclease superfamily. It is exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. It degrades RNA at a rate that is approximately 35-fold hi 0.1 mg
EIAAB34334 Exonuclease XPMC2,Gm111,Mouse,Mus musculus,Pmc2,Prevents mitotic catastrophe 2 protein homolog,Rexo4,RNA exonuclease 4,Xpmc2h
EIAAB34333 Exonuclease XPMC2,Homo sapiens,hPMC2,Human,PMC2,Prevents mitotic catastrophe 2 protein homolog,REXO4,RNA exonuclease 4,XPMC2H
29-514 THEX1 contains 1 SAP domain and 1 exonuclease domain. It is an RNA exonuclease that binds to the 3' end of histone mRNAs and probably degrades them, suggesting that it plays an essential role in histo 0.1 mg
29-513 THEX1 contains 1 SAP domain and 1 exonuclease domain. It is an RNA exonuclease that binds to the 3' end of histone mRNAs and probably degrades them, suggesting that it plays an essential role in histo 0.05 mg
EIAAB13234 3'-5' exonuclease ERI1,3'-5' exoribonuclease 1,3'exo,Eri1,Eri-1 homolog,Histone mRNA 3'-exonuclease 1,Mouse,Mus musculus,Thex1
EIAAB43821 3'-5' exonuclease TREX1,DNase III,Homo sapiens,Human,Three prime repair exonuclease 1,TREX1
EIAAB43824 3'-5' exonuclease TREX2,Homo sapiens,Human,Three prime repair exonuclease 2,TREX2
A-1111-050 CRISPR per Cas9 Polyclonal Antibody 50
A-1111-100 CRISPR per Cas9 Polyclonal Antibody 100
A-1111-010 CRISPR per Cas9 Polyclonal Antibody 10
A-9000-050 CRISPR per Cas9 Monoclonal Antibody 50
A-9000-010 CRISPR per Cas9 Monoclonal Antibody 10
A-9000-100 CRISPR per Cas9 Monoclonal Antibody 100
EIAAB43823 3'-5' exonuclease TREX1,Mouse,Mus musculus,Three prime repair exonuclease 1,Trex1
EIAAB43825 3'-5' exonuclease TREX2,Mouse,Mus musculus,Three prime repair exonuclease 2,Trex2
EIAAB43822 3'-5' exonuclease TREX1,Bos taurus,Bovine,Three prime repair exonuclease 1,TREX1
S303 CRISPR per Cas9 and TALEN vectors validation services sample
S303 CRISPR.Cas9 and TALEN vectors validation services genomic DNA sample


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur