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Chloramphenicol 3-O phosphotransferase (CPT) (EC 2.7.1.-)

 CPT_STRVP               Reviewed;         178 AA.
Q56148; F2RH22;
27-APR-2001, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
22-NOV-2017, entry version 96.
RecName: Full=Chloramphenicol 3-O phosphotransferase;
Short=CPT;
EC=2.7.1.-;
OrderedLocusNames=SVEN_4064;
Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 /
JCM 4526 / NBRC 13096 / PD 04745).
Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
Streptomyces.
NCBI_TaxID=953739;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 /
PD 04745;
PubMed=7592948; DOI=10.1074/jbc.270.45.27000;
Mosher R.H., Camp D.J., Yang K., Brown M.P., Shaw W.V., Vining L.C.;
"Inactivation of chloramphenicol by O-phosphorylation. A novel
resistance mechanism in Streptomyces venezuelae ISP5230, a
chloramphenicol producer.";
J. Biol. Chem. 270:27000-27006(1995).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 /
PD 04745;
PubMed=21463507; DOI=10.1186/1471-2164-12-175;
Pullan S.T., Chandra G., Bibb M.J., Merrick M.;
"Genome-wide analysis of the role of GlnR in Streptomyces venezuelae
provides new insights into global nitrogen regulation in
actinomycetes.";
BMC Genomics 12:175-175(2011).
[3]
X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
STRAIN=ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 /
PD 04745;
PubMed=10835366; DOI=10.1093/emboj/19.11.2690;
Izard T., Ellis J.;
"The crystal structures of chloramphenicol phosphotransferase reveal a
novel inactivation mechanism.";
EMBO J. 19:2690-2700(2000).
-!- FUNCTION: Inactivates chloramphenicol by catalyzing the transfer
of the gamma-phosphate of ATP to the antibiotic's C-3' hydroxyl
group.
-!- SUBUNIT: Homodimer.
-!- SIMILARITY: To M.tuberculosis Rv2636. {ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=CCA57350.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
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EMBL; U09991; AAB36569.1; -; Genomic_DNA.
EMBL; FR845719; CCA57350.1; ALT_INIT; Genomic_DNA.
RefSeq; WP_063844287.1; NG_047672.1.
PDB; 1GRQ; X-ray; 2.90 A; A=1-178.
PDB; 1GRR; X-ray; 2.90 A; A=1-178.
PDB; 1QHN; X-ray; 2.70 A; A=1-178.
PDB; 1QHS; X-ray; 2.80 A; A=1-178.
PDB; 1QHX; X-ray; 2.50 A; A=1-178.
PDB; 1QHY; X-ray; 2.60 A; A=1-178.
PDBsum; 1GRQ; -.
PDBsum; 1GRR; -.
PDBsum; 1QHN; -.
PDBsum; 1QHS; -.
PDBsum; 1QHX; -.
PDBsum; 1QHY; -.
ProteinModelPortal; Q56148; -.
SMR; Q56148; -.
DrugBank; DB04411; Alpha-N-Dichloroacetyl-P-Aminophenylserinol.
DrugBank; DB00446; Chloramphenicol.
DrugBank; DB02608; N-Acetyl-P-Nitrophenylserinol.
DrugBank; DB02930; Phosphothiophosphoric Acid-Adenylate Ester.
EnsemblBacteria; CCA57350; CCA57350; SVEN_4064.
KEGG; ag:AAB36569; -.
KEGG; sve:SVEN_4064; -.
PATRIC; fig|953739.5.peg.6564; -.
KO; K18554; -.
OrthoDB; POG091H0L09; -.
EvolutionaryTrace; Q56148; -.
Proteomes; UP000006854; Chromosome.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
CDD; cd00227; CPT; 1.
InterPro; IPR012853; CPT.
InterPro; IPR027417; P-loop_NTPase.
Pfam; PF07931; CPT; 1.
PIRSF; PIRSF007531; CPT; 1.
SUPFAM; SSF52540; SSF52540; 1.
1: Evidence at protein level;
3D-structure; Antibiotic resistance; ATP-binding; Complete proteome;
Kinase; Nucleotide-binding; Reference proteome; Transferase.
CHAIN 1 178 Chloramphenicol 3-O phosphotransferase.
/FTId=PRO_0000079295.
NP_BIND 10 17 ATP.
ACT_SITE 37 37
STRAND 5 9 {ECO:0000244|PDB:1QHX}.
HELIX 16 26 {ECO:0000244|PDB:1QHX}.
STRAND 27 29 {ECO:0000244|PDB:1QHX}.
STRAND 31 35 {ECO:0000244|PDB:1QHX}.
HELIX 36 42 {ECO:0000244|PDB:1QHX}.
HELIX 45 48 {ECO:0000244|PDB:1QHX}.
STRAND 53 56 {ECO:0000244|PDB:1QHX}.
TURN 58 60 {ECO:0000244|PDB:1GRQ}.
STRAND 62 64 {ECO:0000244|PDB:1QHX}.
HELIX 66 84 {ECO:0000244|PDB:1QHX}.
STRAND 88 93 {ECO:0000244|PDB:1QHX}.
TURN 96 98 {ECO:0000244|PDB:1QHX}.
HELIX 99 109 {ECO:0000244|PDB:1QHX}.
STRAND 114 120 {ECO:0000244|PDB:1QHX}.
HELIX 123 132 {ECO:0000244|PDB:1QHX}.
STRAND 134 136 {ECO:0000244|PDB:1QHX}.
HELIX 140 144 {ECO:0000244|PDB:1QHX}.
HELIX 147 149 {ECO:0000244|PDB:1QHX}.
STRAND 155 159 {ECO:0000244|PDB:1QHX}.
TURN 160 162 {ECO:0000244|PDB:1GRQ}.
HELIX 165 173 {ECO:0000244|PDB:1QHX}.
SEQUENCE 178 AA; 18816 MW; FC12D650A1C5D51A CRC64;
MTTRMIILNG GSSAGKSGIV RCLQSVLPEP WLAFGVDSLI EAMPLKMQSA EGGIEFDADG
GVSIGPEFRA LEGAWAEGVV AMARAGARII IDDVFLGGAA AQERWRSFVG DLDVLWVGVR
CDGAVAEGRE TARGDRVAGM AAKQAYVVHE GVEYDVEVDT THKESIECAW AIAAHVVP


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