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Chloramphenicol acetyltransferase (CAT) (EC 2.3.1.28)

 CAT_STRAG               Reviewed;         215 AA.
Q03058;
01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
01-JUL-1993, sequence version 1.
05-DEC-2018, entry version 80.
RecName: Full=Chloramphenicol acetyltransferase;
Short=CAT;
EC=2.3.1.28;
Name=cat;
Streptococcus agalactiae.
Plasmid pIP501.
Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
Streptococcus.
NCBI_TaxID=1311;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1461942; DOI=10.1016/0147-619X(92)90060-N;
Trieu-Cuot P., de Cespedes G., Horaud T.;
"Nucleotide sequence of the chloramphenicol resistance determinant of
the streptococcal plasmid pIP501.";
Plasmid 28:272-276(1992).
-!- FUNCTION: This enzyme is an effector of chloramphenicol resistance
in bacteria.
-!- CATALYTIC ACTIVITY:
Reaction=acetyl-CoA + chloramphenicol = chloramphenicol 3-acetate
+ CoA; Xref=Rhea:RHEA:18421, ChEBI:CHEBI:16730,
ChEBI:CHEBI:17698, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288;
EC=2.3.1.28; Evidence={ECO:0000255|PROSITE-ProRule:PRU10021};
-!- SUBUNIT: Homotrimer.
-!- SIMILARITY: Belongs to the chloramphenicol acetyltransferase
family. {ECO:0000305}.
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EMBL; X65462; CAA46455.1; -; Genomic_DNA.
PIR; JQ1951; JQ1951.
RefSeq; NP_044466.1; NC_001797.1.
RefSeq; WP_002331392.1; NZ_MVFG01000004.1.
ProteinModelPortal; Q03058; -.
SMR; Q03058; -.
GeneID; 1113365; -.
GO; GO:0008811; F:chloramphenicol O-acetyltransferase activity; IEA:UniProtKB-EC.
GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
Gene3D; 3.30.559.10; -; 1.
InterPro; IPR023213; CAT-like_dom_sf.
InterPro; IPR018372; Chloramphenicol_AcTrfase_AS.
InterPro; IPR001707; Cmp_AcTrfase.
PANTHER; PTHR38474; PTHR38474; 1.
Pfam; PF00302; CAT; 1.
PIRSF; PIRSF000440; CAT; 1.
ProDom; PD002660; Cmp_AcTrfase; 1.
SMART; SM01059; CAT; 1.
PROSITE; PS00100; CAT; 1.
3: Inferred from homology;
Acyltransferase; Antibiotic resistance; Plasmid; Transferase.
CHAIN 1 215 Chloramphenicol acetyltransferase.
/FTId=PRO_0000165879.
ACT_SITE 189 189 Proton acceptor. {ECO:0000255|PROSITE-
ProRule:PRU10021}.
SEQUENCE 215 AA; 25789 MW; 7FD30D9B612BB7B9 CRC64;
MTFNIIELEN WDRKEYFEHY FNQQTTYSIT KEIDITLFKD MIKKKGYEIY PSLIYAIMEV
VNKNKVFRTG INSENKLGYW DKLNPLYTVF NKQTEKFTNI WTESDKNFIS FYNNYKNDLL
EYKDKEEMFP KKPIPENTIP ISMIPWIDFS SFNLNIGNNS SFLLPIITIG KFYSENNKIY
IPVALQLHHS VCDGYHASLF MNEFQDIIHR VDDWI


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