Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Chlorophyll synthase, chloroplastic (EC 2.5.1.62) (Polyprenyl transferase)

 CHLG_AVESA              Reviewed;         378 AA.
Q9M3W5;
01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
01-OCT-2000, sequence version 1.
05-DEC-2018, entry version 59.
RecName: Full=Chlorophyll synthase, chloroplastic;
EC=2.5.1.62;
AltName: Full=Polyprenyl transferase;
Flags: Precursor;
Name=CHLG;
Avena sativa (Oat).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP clade;
Pooideae; Poodae; Poeae; Aveninae; Avena.
NCBI_TaxID=4498;
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INHIBITION BY N-PHENYLMALEIMIDE
AND DIACETYL, AND MUTAGENESIS OF ARG-91; CYS-109; CYS-130; CYS-137;
ARG-151; ARG-161; CYS-262; ARG-284 AND CYS-304.
PubMed=11501754; DOI=10.1515/BC.2001.112;
Schmid H.C., Oster U., Koegel J., Lenz S., Ruediger W.;
"Cloning and characterisation of chlorophyll synthase from Avena
sativa.";
Biol. Chem. 382:903-911(2001).
[2]
CHARACTERIZATION, AND MUTAGENESIS OF ASN-146; ASP-147; ASP-150;
ASP-154 AND ARG-161.
STRAIN=cv. Pirol;
PubMed=12530542; DOI=10.1515/BC.2002.198;
Schmid H.C., Rassadina V., Oster U., Schoch S., Ruediger W.;
"Pre-loading of chlorophyll synthase with tetraprenyl diphosphate is
an obligatory step in chlorophyll biosynthesis.";
Biol. Chem. 383:1769-1778(2002).
-!- FUNCTION: Involved in one of the last steps of the biosynthesis of
chlorophyll a. Catalyzes the esterification of chlorophillide a
with either geranylgeranyldiphosphate (GGPP) or phytyldiphosphate
(PhyPP). May also use with a lower efficiency the monophosphates
GGMP and PhyMP, but not the non-phosphorylated alcohols
geranylgeraniol and phytol. The tetraprenyl diphosphate must bind
to the enzyme as the first substrate and esterification occurs
when this pre-loaded enzyme meets the second substrate,
chlorophyllide. {ECO:0000269|PubMed:11501754}.
-!- CATALYTIC ACTIVITY:
Reaction=chlorophyllide a + H(+) + phytyl diphosphate =
chlorophyll a + diphosphate; Xref=Rhea:RHEA:17317,
ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:58416,
ChEBI:CHEBI:75434, ChEBI:CHEBI:83348; EC=2.5.1.62;
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
Note=Magnesium or zinc; or manganese at a lesser extent, but not
calcium.;
-!- ACTIVITY REGULATION: Inhibited by N-phenylmaleimide (NPM) and
diacetyl.
-!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
Multi-pass membrane protein {ECO:0000305}.
-!- DEVELOPMENTAL STAGE: Constitutively expressed in etiolated and
green seedlings.
-!- DOMAIN: The N-terminal part (1-87) is not required for enzymatic
activity.
-!- DOMAIN: Amino acid residues 138-160 are probably part of the
polyprenyl diphosphate-binding domain.
-!- SIMILARITY: Belongs to the UbiA prenyltransferase family.
Chlorophyll synthase subfamily. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; AJ277210; CAB85464.1; -; mRNA.
PRIDE; Q9M3W5; -.
KEGG; ag:CAB85464; -.
KO; K04040; -.
BioCyc; MetaCyc:MONOMER-11751; -.
GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
GO; GO:0046408; F:chlorophyll synthetase activity; IEA:UniProtKB-EC.
GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
CDD; cd13958; PT_UbiA_chlorophyll; 1.
InterPro; IPR006372; Chl_synth.
InterPro; IPR011799; ChlG.
InterPro; IPR000537; UbiA_prenyltransferase.
Pfam; PF01040; UbiA; 1.
TIGRFAMs; TIGR02056; ChlG; 1.
TIGRFAMs; TIGR01476; chlor_syn_BchG; 1.
1: Evidence at protein level;
Chlorophyll biosynthesis; Chloroplast; Membrane; Plastid; Transferase;
Transit peptide; Transmembrane; Transmembrane helix.
TRANSIT 1 45 Chloroplast. {ECO:0000255}.
CHAIN 46 378 Chlorophyll synthase, chloroplastic.
/FTId=PRO_0000285116.
TRANSMEM 173 193 Helical. {ECO:0000255}.
TRANSMEM 199 219 Helical. {ECO:0000255}.
TRANSMEM 232 252 Helical. {ECO:0000255}.
TRANSMEM 257 277 Helical. {ECO:0000255}.
TRANSMEM 302 322 Helical. {ECO:0000255}.
TRANSMEM 327 347 Helical. {ECO:0000255}.
TRANSMEM 357 377 Helical. {ECO:0000255}.
MUTAGEN 91 91 R->A: Total loss of activity.
{ECO:0000269|PubMed:11501754}.
MUTAGEN 109 109 C->A: Reduces activity by 90%.
{ECO:0000269|PubMed:11501754}.
MUTAGEN 130 130 C->A: Reduces activity by 70%.
{ECO:0000269|PubMed:11501754}.
MUTAGEN 137 137 C->A: No effect.
{ECO:0000269|PubMed:11501754}.
MUTAGEN 146 146 N->A: Total loss of activity.
{ECO:0000269|PubMed:12530542}.
MUTAGEN 147 147 D->A: Total loss of activity.
{ECO:0000269|PubMed:12530542}.
MUTAGEN 150 150 D->A: Total loss of activity.
{ECO:0000269|PubMed:12530542}.
MUTAGEN 151 151 R->A: Reduces activity by 75%.
{ECO:0000269|PubMed:11501754}.
MUTAGEN 154 154 D->A: Total loss of activity.
{ECO:0000269|PubMed:12530542}.
MUTAGEN 161 161 R->A: Total loss of activity.
{ECO:0000269|PubMed:11501754,
ECO:0000269|PubMed:12530542}.
MUTAGEN 161 161 R->H: Reduces activity by 99%.
{ECO:0000269|PubMed:11501754,
ECO:0000269|PubMed:12530542}.
MUTAGEN 161 161 R->K: Reduces activity by 66%.
{ECO:0000269|PubMed:11501754,
ECO:0000269|PubMed:12530542}.
MUTAGEN 262 262 C->A: No effect.
{ECO:0000269|PubMed:11501754}.
MUTAGEN 284 284 R->A: No effect.
{ECO:0000269|PubMed:11501754}.
MUTAGEN 304 304 C->A: Loss of inhibition by N-
phenylmaleimide, but no loss of activity.
{ECO:0000269|PubMed:11501754}.
SEQUENCE 378 AA; 40764 MW; B7FEABF82D061DE4 CRC64;
MATSHPLAAA AATSSSSATF RPPLRFLSSP PSSLTLNRRR SFPVVCAADA DAKETTKKPT
IPDKAPAAGS SFNQLLGIKG AKQETNIWKI RLQLTKPVTW PPLVWGVLCG AAASGNFHWT
VEDVTKSIVC MLMSGPCLTG YTQTINDWYD RDIDAINEPY RPIPSGAISE NEVITQIWVL
LLGGLGLGAL LDIWAGHDFP IIFYLALGGS LLSYIYSAPP LKLKQNGWIG NFALGASYIG
LPWWAGQALF GTLTPDIVVL TCLYSIAGLG IAIVNDFKSI EGDRTLGLQS LPVAFGMETA
KWICVGAIDI TQLSVAAYLL STGKLYYALA LLGLTIPQVI LQFQYFLKDP VKYDVKYQAS
AQPFFVFGLL VTALATSH


Related products :

Catalog number Product name Quantity
AS09 579 ATP synthase subunit III_c, chloroplastic 100 ul
26-361 Dehydrodolichyl diphosphate (dedol-PP) synthase catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier lipid required for the biosynthesis of several 0.05 mg
abx109165 Polyclonal Rabbit ATP synthase subunit beta, chloroplastic Antibody (HRP) 100 μg
CSB-RP139994Pl Recombinant Lemna minor ATP synthase subunit beta, chloroplastic 500ug
abx106334 Polyclonal Rabbit ATP synthase subunit beta, chloroplastic Antibody (Biotin) 100 μg
AS14 2782 rabbit polyclonal EPSP synthase | 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic 50
abx107747 Polyclonal Rabbit ATP synthase subunit beta, chloroplastic Antibody (FITC) 100 μg
U1045m CLIA D5Ertd135e,Mouse,Mus musculus,O-phosphoseryl-tRNA(Sec) selenium transferase,Sec synthase,Selenocysteine synthase,Selenocysteinyl-tRNA(Sec) synthase,SepSecS,Sepsecs,Sep-tRNA Sec-tRNA synthase,UGA 96T
E1045m ELISA D5Ertd135e,Mouse,Mus musculus,O-phosphoseryl-tRNA(Sec) selenium transferase,Sec synthase,Selenocysteine synthase,Selenocysteinyl-tRNA(Sec) synthase,SepSecS,Sepsecs,Sep-tRNA Sec-tRNA synthase,UGA 96T
CSB-RP139994Pl Recombinant Lemna minor ATP synthase subunit beta, chloroplastic Source: E.coli 1mg
CSB-EL027234PL Plant solanesyl diphosphate synthase 2, chloroplastic(SPS2_SPPS)ELISA kit , Species , Sample Type 96T
U1045h CLIA Homo sapiens,Human,Liver-pancreas antigen,LP,O-phosphoseryl-tRNA(Sec) selenium transferase,Sec synthase,Selenocysteine synthase,Selenocysteinyl-tRNA(Sec) synthase,SepSecS,SEPSECS,Sep-tRNA Sec-tRN 96T
E1045h ELISA Homo sapiens,Human,Liver-pancreas antigen,LP,O-phosphoseryl-tRNA(Sec) selenium transferase,Sec synthase,Selenocysteine synthase,Selenocysteinyl-tRNA(Sec) synthase,SepSecS,SEPSECS,Sep-tRNA Sec-tR 96T
E1045h ELISA kit Homo sapiens,Human,Liver-pancreas antigen,LP,O-phosphoseryl-tRNA(Sec) selenium transferase,Sec synthase,Selenocysteine synthase,Selenocysteinyl-tRNA(Sec) synthase,SepSecS,SEPSECS,Sep-tRNA S 96T
E1045m ELISA kit D5Ertd135e,Mouse,Mus musculus,O-phosphoseryl-tRNA(Sec) selenium transferase,Sec synthase,Selenocysteine synthase,Selenocysteinyl-tRNA(Sec) synthase,SepSecS,Sepsecs,Sep-tRNA Sec-tRNA synthas 96T
H1805 Solanesyl diphosphate synthase 2, chloroplastic (SPS2 SPPS)ELISA kit, Plant, ELISA Kit 96T
CSB-EL027234PL Plant solanesyl diphosphate synthase 2, chloroplastic(SPS2_SPPS)ELISA kit, Species Plant, Sample Type serum, plasma 96T
AS11 1757 Antibody: Fd | ferredoxin 1 (chloroplastic), Immunogen: KLH-conjugated synthetic peptide derived from known C-terminal part of Chlamydomonas reinhardtii chloroplastic ferredoxin P07839, Host: rabbit, 200
AS08 348 Antibody: HSP70 | heat shock protein 70, chloroplastic , Immunogen: KLH-conjugated synthetic peptide derived from higher plant chloroplastic HSP70, inluding Arabidopsis thalianacpHSC70-1 and cpHSC70-2 200
AS09 457 Antibody: AtpG | ATPsynthase subunit II b', chloroplastic, Immunogen: KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8 and Chlamydomonas reinhardtii ATP synthase s 100
EIAAB32966 Homo sapiens,Human,MGST1L1,MGST1-L1,Microsomal glutathione S-transferase 1-like 1,Microsomal prostaglandin E synthase 1,MPGES1,MPGES-1,p53-induced gene 12 protein,PGES,PIG12,Prostaglandin E synthase,P
9030-11-9 beta_1,4_Galactosyl Transferase from bo Lactose Synthase 1g
U0609m CLIA Glutathione S-transferase A1,Glutathione S-transferase Ya,Glutathione S-transferase Ya1,GST class-alpha member 1,Gsta,Gsta1,Gstya,Mouse,Mus musculus 96T
E0609m ELISA kit Glutathione S-transferase A1,Glutathione S-transferase Ya,Glutathione S-transferase Ya1,GST class-alpha member 1,Gsta,Gsta1,Gstya,Mouse,Mus musculus 96T
E0609m ELISA Glutathione S-transferase A1,Glutathione S-transferase Ya,Glutathione S-transferase Ya1,GST class-alpha member 1,Gsta,Gsta1,Gstya,Mouse,Mus musculus 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur