Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Cholesterol 7-alpha-monooxygenase (EC 1.14.14.23) (CYPVII) (Cholesterol 7-alpha-hydroxylase) (Cytochrome P450 7A1)

 CP7A1_RAT               Reviewed;         503 AA.
P18125; P51543;
01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
01-NOV-1990, sequence version 1.
28-FEB-2018, entry version 148.
RecName: Full=Cholesterol 7-alpha-monooxygenase;
EC=1.14.14.23 {ECO:0000269|PubMed:1694852, ECO:0000269|PubMed:2335522};
AltName: Full=CYPVII;
AltName: Full=Cholesterol 7-alpha-hydroxylase;
AltName: Full=Cytochrome P450 7A1;
Name=Cyp7a1; Synonyms=Cyp7;
Rattus norvegicus (Rat).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
NCBI_TaxID=10116;
[1]
NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION, INDUCTION,
SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
STRAIN=Sprague-Dawley; TISSUE=Liver;
PubMed=1694852;
Li Y.C., Wang D.P., Chiang J.Y.L.;
"Regulation of cholesterol 7 alpha-hydroxylase in the liver. Cloning,
sequencing, and regulation of cholesterol 7 alpha-hydroxylase mRNA.";
J. Biol. Chem. 265:12012-12019(1990).
[2]
NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CATALYTIC
ACTIVITY, FUNCTION, INDUCTION, AND TISSUE SPECIFICITY.
STRAIN=Sprague-Dawley;
PubMed=2335522;
Jelinek D.F., Andersson S., Slaughter C.A., Russell D.W.;
"Cloning and regulation of cholesterol 7 alpha-hydroxylase, the rate-
limiting enzyme in bile acid biosynthesis.";
J. Biol. Chem. 265:8190-8197(1990).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2806567; DOI=10.1016/0014-5793(89)81795-8;
Noshiro M., Nishimoto M., Morohashi K., Okuda K.;
"Molecular cloning of cDNA for cholesterol 7 alpha-hydroxylase from
rat liver microsomes. Nucleotide sequence and expression.";
FEBS Lett. 257:97-100(1989).
[4]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Liver;
PubMed=1693613;
Noshiro M., Nishimoto M., Okuda K.;
"Rat liver cholesterol 7 alpha-hydroxylase. Pretranslational
regulation for circadian rhythm.";
J. Biol. Chem. 265:10036-10041(1990).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2007596;
Nishimoto M., Gotoh O., Okuda K., Noshiro M.;
"Structural analysis of the gene encoding rat cholesterol alpha-
hydroxylase, the key enzyme for bile acid biosynthesis.";
J. Biol. Chem. 266:6467-6471(1991).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-325.
STRAIN=Sprague-Dawley;
PubMed=8021257;
Chiang J.Y., Stroup D.;
"Identification and characterization of a putative bile acid-
responsive element in cholesterol 7 alpha-hydroxylase gene promoter.";
J. Biol. Chem. 269:17502-17507(1994).
[7]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
STRAIN=Sprague-Dawley;
PubMed=2261433; DOI=10.1021/bi00486a001;
Jelinek D.F., Russell D.W.;
"Structure of the rat gene encoding cholesterol 7 alpha-hydroxylase.";
Biochemistry 29:7781-7785(1990).
[8]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.
PubMed=1420318; DOI=10.1016/0167-4781(92)90175-Y;
Chiang J.Y., Yang T.P., Wang D.P.;
"Cloning and 5'-flanking sequence of a rat cholesterol 7 alpha-
hydroxylase gene.";
Biochim. Biophys. Acta 1132:337-339(1992).
[9]
INDUCTION.
PubMed=11278771; DOI=10.1074/jbc.M010878200;
Gupta S., Stravitz R.T., Dent P., Hylemon P.B.;
"Down-regulation of cholesterol 7alpha-hydroxylase (CYP7A1) gene
expression by bile acids in primary rat hepatocytes is mediated by the
c-Jun N-terminal kinase pathway.";
J. Biol. Chem. 276:15816-15822(2001).
-!- FUNCTION: Catalyzes a rate-limiting step in cholesterol catabolism
and bile acid biosynthesis by introducing a hydrophilic moiety at
position 7 of cholesterol. Important for cholesterol homeostasis.
{ECO:0000269|PubMed:1694852, ECO:0000269|PubMed:2335522}.
-!- CATALYTIC ACTIVITY: Cholesterol + [reduced NADPH--hemoprotein
reductase] + O(2) = 7-alpha-hydroxycholesterol + [oxidized NADPH--
hemoprotein reductase] + H(2)O. {ECO:0000269|PubMed:1694852,
ECO:0000269|PubMed:2335522}.
-!- COFACTOR:
Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
-!- ENZYME REGULATION: Inhibition of activity by bile acid feedback.
-!- PATHWAY: Lipid metabolism; bile acid biosynthesis.
-!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
{ECO:0000269|PubMed:1694852}; Peripheral membrane protein
{ECO:0000269|PubMed:1694852}. Microsome membrane
{ECO:0000269|PubMed:1694852}; Peripheral membrane protein
{ECO:0000269|PubMed:1694852}.
-!- TISSUE SPECIFICITY: Detected in liver (at protein level). Liver.
{ECO:0000269|PubMed:1694852, ECO:0000269|PubMed:2335522}.
-!- INDUCTION: Up-regulated by dietary cholesterol and cholestyramine.
Down-regulated by dietary bile acids, such as chenodeoxycholic
acid and cholic acid. {ECO:0000269|PubMed:11278771,
ECO:0000269|PubMed:1694852, ECO:0000269|PubMed:2335522}.
-!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; J05509; AAA40839.1; -; mRNA.
EMBL; J02926; AAA40923.1; -; Genomic_DNA.
EMBL; J05430; AAA41041.1; -; mRNA.
EMBL; J05460; AAA03649.1; -; mRNA.
EMBL; M59189; AAA41042.1; -; Genomic_DNA.
EMBL; M59184; AAA41042.1; JOINED; Genomic_DNA.
EMBL; M59185; AAA41042.1; JOINED; Genomic_DNA.
EMBL; M59186; AAA41042.1; JOINED; Genomic_DNA.
EMBL; M59187; AAA41042.1; JOINED; Genomic_DNA.
EMBL; M59188; AAA41042.1; JOINED; Genomic_DNA.
EMBL; U01962; AAA21144.2; -; Genomic_DNA.
EMBL; X17595; CAB57878.1; -; mRNA.
EMBL; Z14108; CAA78481.1; -; Genomic_DNA.
PIR; A35376; A35376.
RefSeq; NP_037074.1; NM_012942.2.
UniGene; Rn.10737; -.
ProteinModelPortal; P18125; -.
SMR; P18125; -.
STRING; 10116.ENSRNOP00000012819; -.
BindingDB; P18125; -.
ChEMBL; CHEMBL2339; -.
SwissLipids; SLP:000001323; -.
PaxDb; P18125; -.
PRIDE; P18125; -.
Ensembl; ENSRNOT00000012819; ENSRNOP00000012819; ENSRNOG00000009488.
GeneID; 25428; -.
KEGG; rno:25428; -.
UCSC; RGD:2482; rat.
CTD; 1581; -.
RGD; 2482; Cyp7a1.
eggNOG; KOG0684; Eukaryota.
eggNOG; COG2124; LUCA.
GeneTree; ENSGT00550000074551; -.
HOGENOM; HOG000231026; -.
HOVERGEN; HBG051100; -.
InParanoid; P18125; -.
KO; K00489; -.
OMA; HGHVFTC; -.
OrthoDB; EOG091G07UI; -.
PhylomeDB; P18125; -.
TreeFam; TF105090; -.
BioCyc; MetaCyc:MONOMER-14293; -.
BRENDA; 1.14.13.17; 5301.
Reactome; R-RNO-192105; Synthesis of bile acids and bile salts.
Reactome; R-RNO-193368; Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
Reactome; R-RNO-193807; Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
Reactome; R-RNO-211976; Endogenous sterols.
SABIO-RK; P18125; -.
UniPathway; UPA00221; -.
PRO; PR:P18125; -.
Proteomes; UP000002494; Chromosome 5.
Bgee; ENSRNOG00000009488; -.
Genevisible; P18125; RN.
GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:UniProtKB.
GO; GO:0031090; C:organelle membrane; IEA:UniProtKB-SubCell.
GO; GO:0008123; F:cholesterol 7-alpha-monooxygenase activity; IDA:UniProtKB.
GO; GO:0020037; F:heme binding; IEA:InterPro.
GO; GO:0005506; F:iron ion binding; IEA:InterPro.
GO; GO:0006699; P:bile acid biosynthetic process; IDA:UniProtKB.
GO; GO:0071397; P:cellular response to cholesterol; IDA:UniProtKB.
GO; GO:0071333; P:cellular response to glucose stimulus; ISS:UniProtKB.
GO; GO:0006707; P:cholesterol catabolic process; IDA:UniProtKB.
GO; GO:0042632; P:cholesterol homeostasis; IDA:UniProtKB.
GO; GO:0070857; P:regulation of bile acid biosynthetic process; IDA:UniProtKB.
Gene3D; 1.10.630.10; -; 1.
InterPro; IPR030681; Cholesterol_7a_monooxygenase.
InterPro; IPR001128; Cyt_P450.
InterPro; IPR017972; Cyt_P450_CS.
InterPro; IPR024204; Cyt_P450_CYP7A1-type.
InterPro; IPR002403; Cyt_P450_E_grp-IV.
InterPro; IPR036396; Cyt_P450_sf.
PANTHER; PTHR24304:SF1; PTHR24304:SF1; 1.
Pfam; PF00067; p450; 1.
PIRSF; PIRSF500625; Cytochrome_CYP7A1; 1.
PIRSF; PIRSF000047; Cytochrome_CYPVIIA1; 1.
PRINTS; PR00465; EP450IV.
SUPFAM; SSF48264; SSF48264; 1.
PROSITE; PS00086; CYTOCHROME_P450; 1.
1: Evidence at protein level;
Cholesterol metabolism; Complete proteome; Direct protein sequencing;
Endoplasmic reticulum; Heme; Iron; Lipid metabolism; Membrane;
Metal-binding; Microsome; Monooxygenase; Oxidoreductase;
Reference proteome; Steroid metabolism; Sterol metabolism.
CHAIN 1 503 Cholesterol 7-alpha-monooxygenase.
/FTId=PRO_0000051905.
METAL 444 444 Iron (heme axial ligand). {ECO:0000250}.
CONFLICT 371 371 T -> S (in Ref. 5; AAA41042).
{ECO:0000305}.
SEQUENCE 503 AA; 56883 MW; EA825AA6E74BF5F6 CRC64;
MMTISLIWGI AVLVSCCIWF IVGIRRRKAG EPPLENGLIP YLGCALKFGS NPLEFLRANQ
RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF HYTTSAKAFG HRSIDPNDGN
TTENINNTFT KTLQGDALCS LSEAMMQNLQ SVMRPPGLPK SKSNAWVTEG MYAFCYRVMF
EAGYLTLFGR DISKTDTQKA LILNNLDNFK QFDQVFPALV AGLPIHLFKT AHKAREKLAE
GLKHKNLCVR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL AILWASQANT IPATFWSLFQ
MIRSPEAMKA ASEEVSGALQ SAGQELSSGG SAIYLDQVQL NDLPVLDSII KEALRLSSAS
LNIRTAKEDF TLHLEDGSYN IRKDDMIALY PQLMHLDPEI YPDPLTFKYD RYLDESGKAK
TTFYSNGNKL KCFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSCFELEFV ESQVKCPPLD
QSRAGLGILP PLHDIEFKYK LKH


Related products :

Catalog number Product name Quantity
E1053p ELISA Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,CYP7,CYP7A1,CYPVII,Cytochrome P450 7A1,Pig,Sus scrofa 96T
U1053p CLIA Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,CYP7,CYP7A1,CYPVII,Cytochrome P450 7A1,Pig,Sus scrofa 96T
E1053p ELISA kit Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,CYP7,CYP7A1,CYPVII,Cytochrome P450 7A1,Pig,Sus scrofa 96T
E1053m ELISA kit Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,Cyp7,Cyp7a1,CYPVII,Cytochrome P450 7A1,Mouse,Mus musculus 96T
E1053r ELISA Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,Cyp7,Cyp7a1,CYPVII,Cytochrome P450 7A1,Rat,Rattus norvegicus 96T
U1053r CLIA Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,Cyp7,Cyp7a1,CYPVII,Cytochrome P450 7A1,Rat,Rattus norvegicus 96T
E1053r ELISA kit Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,Cyp7,Cyp7a1,CYPVII,Cytochrome P450 7A1,Rat,Rattus norvegicus 96T
U1053m CLIA Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,Cyp7,Cyp7a1,CYPVII,Cytochrome P450 7A1,Mouse,Mus musculus 96T
E1053m ELISA Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,Cyp7,Cyp7a1,CYPVII,Cytochrome P450 7A1,Mouse,Mus musculus 96T
E1053Rb ELISA kit Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,CYP7,CYP7A1,CYPVII,Cytochrome P450 7A1,Oryctolagus cuniculus,Rabbit 96T
E1053Rb ELISA Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,CYP7,CYP7A1,CYPVII,Cytochrome P450 7A1,Oryctolagus cuniculus,Rabbit 96T
E1053h ELISA Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,CYP7,CYP7A1,CYPVII,Cytochrome P450 7A1,Homo sapiens,Human 96T
U1053h CLIA Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,CYP7,CYP7A1,CYPVII,Cytochrome P450 7A1,Homo sapiens,Human 96T
E1053h ELISA kit Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,CYP7,CYP7A1,CYPVII,Cytochrome P450 7A1,Homo sapiens,Human 96T
U1053Rb CLIA Cholesterol 7-alpha-hydroxylase,Cholesterol 7-alpha-monooxygenase,CYP7,CYP7A1,CYPVII,Cytochrome P450 7A1,Oryctolagus cuniculus,Rabbit 96T
EIAAB08889 25-hydroxyvitamin D(3) 1-alpha-hydroxylase,25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial,25-OHD-1 alpha-hydroxylase,Calcidiol 1-monooxygenase,Cyp27b,Cyp27b1,Cytochrome p450 27B1,Cytochrome P4
EIAAB08887 25-hydroxyvitamin D(3) 1-alpha-hydroxylase,25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial,25-OHD-1 alpha-hydroxylase,Calcidiol 1-monooxygenase,Cyp27b,Cyp27b1,Cyp40,Cytochrome p450 27B1,Cytochr
EIAAB08888 25-hydroxyvitamin D(3) 1-alpha-hydroxylase,25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial,25-OHD-1 alpha-hydroxylase,Calcidiol 1-monooxygenase,CYP1ALPHA,CYP27B,CYP27B1,Cytochrome p450 27B1,Cyt
E0987p ELISA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Pig,Sus scrofa 96T
E0987p ELISA kit Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Pig,Sus scrofa 96T
U0987p CLIA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Pig,Sus scrofa 96T
U0987b CLIA Bos taurus,Bovine,Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc) 96T
E0987b ELISA Bos taurus,Bovine,Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc) 96T
E0987b ELISA kit Bos taurus,Bovine,Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc) 96T
E0987Rb ELISA kit Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Oryctolagus cuniculus,Rabbit 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur