Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Cholesterol side-chain cleavage enzyme, mitochondrial (EC 1.14.15.6) (CYPXIA1) (Cholesterol desmolase) (Cytochrome P450 11A1) (Cytochrome P450(scc))

 CP11A_BOVIN             Reviewed;         520 AA.
P00189; Q28152;
21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
21-JUL-1986, sequence version 1.
28-FEB-2018, entry version 133.
RecName: Full=Cholesterol side-chain cleavage enzyme, mitochondrial;
EC=1.14.15.6;
AltName: Full=CYPXIA1;
AltName: Full=Cholesterol desmolase;
AltName: Full=Cytochrome P450 11A1;
AltName: Full=Cytochrome P450(scc);
Flags: Precursor;
Name=CYP11A1;
Bos taurus (Bovine).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
Pecora; Bovidae; Bovinae; Bos.
NCBI_TaxID=9913;
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Adrenal cortex;
PubMed=6589615; DOI=10.1073/pnas.81.15.4647;
Morohashi K., Fujii-Kuriyama Y., Okada Y., Sogawa K., Hirose T.,
Inayama S., Omura T.;
"Molecular cloning and nucleotide sequence of cDNA for mRNA of
mitochondrial cytochrome P-450(SCC) of bovine adrenal cortex.";
Proc. Natl. Acad. Sci. U.S.A. 81:4647-4651(1984).
[2]
PROTEIN SEQUENCE OF 40-520.
PubMed=3518802; DOI=10.1016/0167-4838(86)90176-7;
Chashchin V.L., Lapko V.N., Adamovich T.B., Lapko A.G., Kuprina N.S.,
Akhrem A.A.;
"Primary structure of the cholesterol side-chain cleavage cytochrome
P-450 from bovine adrenocortical mitochondria and some aspects of its
functioning on a structural level.";
Biochim. Biophys. Acta 871:217-223(1986).
[3]
PROTEIN SEQUENCE OF 40-54.
PubMed=6654880;
Ogishima T., Okada Y., Kominami S., Takemori S., Omura T.;
"Partial amino acid sequences of two mitochondrial and two microsomal
cytochrome P-450's from adrenal cortex.";
J. Biochem. 94:1711-1714(1983).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-90.
PubMed=2154474;
Ahlgren R., Simpson E.R., Waterman M.R., Lund J.;
"Characterization of the promoter/regulatory region of the bovine
CYP11A (P-450scc) gene. Basal and cAMP-dependent expression.";
J. Biol. Chem. 265:3313-3319(1990).
[5]
TISSUE SPECIFICITY.
PubMed=3011431;
Hanukoglu I., Hanukoglu Z.;
"Stoichiometry of mitochondrial cytochromes P-450, adrenodoxin and
adrenodoxin reductase in adrenal cortex and corpus luteum.
Implications for membrane organization and gene regulation.";
Eur. J. Biochem. 157:27-31(1986).
[6]
3D-STRUCTURE MODELING OF 40-520.
PubMed=1477100; DOI=10.1016/0167-4838(92)90089-V;
Vijayakumar S., Salerno J.C.;
"Molecular modeling of the 3-D structure of cytochrome P-450scc.";
Biochim. Biophys. Acta 1160:281-286(1992).
[7]
X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 41-520 IN COMPLEX WITH HEME
AND 22R-HYDROXYCHOLESTEROL, AND COFACTOR.
PubMed=21159775; DOI=10.1074/jbc.M110.188433;
Mast N., Annalora A.J., Lodowski D.T., Palczewski K., Stout C.D.,
Pikuleva I.A.;
"Structural basis for three-step sequential catalysis by the
cholesterol side chain cleavage enzyme CYP11A1.";
J. Biol. Chem. 286:5607-5613(2011).
-!- FUNCTION: Catalyzes the side-chain cleavage reaction of
cholesterol to pregnenolone, the precursor of most steroid
hormones. {ECO:0000250|UniProtKB:P05108}.
-!- CATALYTIC ACTIVITY: Cholesterol + 6 reduced adrenodoxin + 3 O(2) +
6 H(+) = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin
+ 4 H(2)O. {ECO:0000250|UniProtKB:P05108}.
-!- COFACTOR:
Name=heme; Xref=ChEBI:CHEBI:30413;
Evidence={ECO:0000269|PubMed:21159775};
-!- PATHWAY: Lipid metabolism; C21-steroid hormone metabolism.
-!- SUBUNIT: Interacts with FDX1/adrenodoxin. {ECO:0000250}.
-!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
{ECO:0000250|UniProtKB:P14137}; Peripheral membrane protein
{ECO:0000305}. Note=Localizes to the matrix side of the
mitochondrion inner membrane. {ECO:0000250|UniProtKB:P14137}.
-!- TISSUE SPECIFICITY: Detected in adrenal cortex and corpus luteum
(at protein level). {ECO:0000269|PubMed:3011431}.
-!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; K02130; AAA30488.1; -; mRNA.
EMBL; J05245; AAA30681.1; -; Genomic_DNA.
PIR; A00189; O4BOM.
RefSeq; NP_788817.1; NM_176644.2.
UniGene; Bt.104995; -.
PDB; 1SCC; Model; -; A=40-520.
PDB; 2ASA; Model; -; A=87-516.
PDB; 3MZS; X-ray; 2.50 A; A/B/C/D=41-520.
PDBsum; 1SCC; -.
PDBsum; 2ASA; -.
PDBsum; 3MZS; -.
ProteinModelPortal; P00189; -.
SMR; P00189; -.
STRING; 9913.ENSBTAP00000009106; -.
BindingDB; P00189; -.
ChEMBL; CHEMBL4813; -.
PaxDb; P00189; -.
PRIDE; P00189; -.
GeneID; 338048; -.
KEGG; bta:338048; -.
CTD; 1583; -.
eggNOG; KOG0159; Eukaryota.
eggNOG; COG2124; LUCA.
HOGENOM; HOG000013161; -.
HOVERGEN; HBG051098; -.
InParanoid; P00189; -.
KO; K00498; -.
BRENDA; 1.14.15.6; 908.
SABIO-RK; P00189; -.
UniPathway; UPA00229; -.
PRO; PR:P00189; -.
Proteomes; UP000009136; Unplaced.
GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
GO; GO:0008386; F:cholesterol monooxygenase (side-chain-cleaving) activity; IDA:UniProtKB.
GO; GO:0020037; F:heme binding; ISS:UniProtKB.
GO; GO:0005506; F:iron ion binding; IEA:InterPro.
GO; GO:0006700; P:C21-steroid hormone biosynthetic process; ISS:UniProtKB.
GO; GO:0008203; P:cholesterol metabolic process; IDA:UniProtKB.
GO; GO:0042359; P:vitamin D metabolic process; IDA:UniProtKB.
Gene3D; 1.10.630.10; -; 1.
InterPro; IPR033283; CYP11A1.
InterPro; IPR001128; Cyt_P450.
InterPro; IPR017972; Cyt_P450_CS.
InterPro; IPR002401; Cyt_P450_E_grp-I.
InterPro; IPR036396; Cyt_P450_sf.
PANTHER; PTHR24279:SF3; PTHR24279:SF3; 1.
Pfam; PF00067; p450; 1.
PRINTS; PR00463; EP450I.
PRINTS; PR00385; P450.
SUPFAM; SSF48264; SSF48264; 1.
PROSITE; PS00086; CYTOCHROME_P450; 1.
1: Evidence at protein level;
3D-structure; Cholesterol metabolism; Complete proteome;
Direct protein sequencing; Heme; Iron; Lipid metabolism; Membrane;
Metal-binding; Mitochondrion; Mitochondrion inner membrane;
Monooxygenase; Oxidoreductase; Reference proteome; Steroid metabolism;
Steroidogenesis; Sterol metabolism; Transit peptide.
TRANSIT 1 39 Mitochondrion.
{ECO:0000269|PubMed:3518802,
ECO:0000269|PubMed:6654880}.
CHAIN 40 520 Cholesterol side-chain cleavage enzyme,
mitochondrial.
/FTId=PRO_0000003582.
METAL 461 461 Iron (heme axial ligand).
{ECO:0000244|PDB:3MZS,
ECO:0000269|PubMed:21159775}.
CONFLICT 22 22 T -> S (in Ref. 4; AAA30681).
{ECO:0000305}.
CONFLICT 57 57 N -> D (in Ref. 2; AA sequence).
{ECO:0000305}.
CONFLICT 106 106 D -> N (in Ref. 2; AA sequence).
{ECO:0000305}.
CONFLICT 197 197 D -> N (in Ref. 2; AA sequence).
{ECO:0000305}.
HELIX 48 50 {ECO:0000244|PDB:3MZS}.
HELIX 58 68 {ECO:0000244|PDB:3MZS}.
HELIX 70 72 {ECO:0000244|PDB:3MZS}.
HELIX 74 85 {ECO:0000244|PDB:3MZS}.
STRAND 87 90 {ECO:0000244|PDB:3MZS}.
STRAND 99 101 {ECO:0000244|PDB:3MZS}.
HELIX 104 112 {ECO:0000244|PDB:3MZS}.
HELIX 124 133 {ECO:0000244|PDB:3MZS}.
TURN 139 141 {ECO:0000244|PDB:3MZS}.
HELIX 145 158 {ECO:0000244|PDB:3MZS}.
TURN 161 167 {ECO:0000244|PDB:3MZS}.
HELIX 168 189 {ECO:0000244|PDB:3MZS}.
STRAND 190 192 {ECO:0000244|PDB:3MZS}.
STRAND 194 196 {ECO:0000244|PDB:3MZS}.
HELIX 199 215 {ECO:0000244|PDB:3MZS}.
STRAND 223 225 {ECO:0000244|PDB:3MZS}.
HELIX 229 242 {ECO:0000244|PDB:3MZS}.
HELIX 243 247 {ECO:0000244|PDB:3MZS}.
HELIX 252 254 {ECO:0000244|PDB:3MZS}.
HELIX 255 258 {ECO:0000244|PDB:3MZS}.
HELIX 260 289 {ECO:0000244|PDB:3MZS}.
HELIX 300 306 {ECO:0000244|PDB:3MZS}.
HELIX 312 343 {ECO:0000244|PDB:3MZS}.
HELIX 345 355 {ECO:0000244|PDB:3MZS}.
TURN 356 362 {ECO:0000244|PDB:3MZS}.
HELIX 366 369 {ECO:0000244|PDB:3MZS}.
HELIX 374 386 {ECO:0000244|PDB:3MZS}.
STRAND 389 391 {ECO:0000244|PDB:3MZS}.
STRAND 393 395 {ECO:0000244|PDB:3MZS}.
STRAND 401 408 {ECO:0000244|PDB:3MZS}.
STRAND 414 416 {ECO:0000244|PDB:3MZS}.
HELIX 418 423 {ECO:0000244|PDB:3MZS}.
TURN 425 427 {ECO:0000244|PDB:3MZS}.
STRAND 428 430 {ECO:0000244|PDB:3MZS}.
HELIX 436 440 {ECO:0000244|PDB:3MZS}.
STRAND 446 449 {ECO:0000244|PDB:3MZS}.
HELIX 457 459 {ECO:0000244|PDB:3MZS}.
HELIX 464 479 {ECO:0000244|PDB:3MZS}.
STRAND 485 488 {ECO:0000244|PDB:3MZS}.
STRAND 494 504 {ECO:0000244|PDB:3MZS}.
STRAND 508 510 {ECO:0000244|PDB:3MZS}.
SEQUENCE 520 AA; 60333 MW; 81C84B4AE0E70418 CRC64;
MLARGLPLRS ALVKACPPIL STVGEGWGHH RVGTGEGAGI STKTPRPYSE IPSPGDNGWL
NLYHFWREKG SQRIHFRHIE NFQKYGPIYR EKLGNLESVY IIHPEDVAHL FKFEGSYPER
YDIPPWLAYH RYYQKPIGVL FKKSGTWKKD RVVLNTEVMA PEAIKNFIPL LNPVSQDFVS
LLHKRIKQQG SGKFVGDIKE DLFHFAFESI TNVMFGERLG MLEETVNPEA QKFIDAVYKM
FHTSVPLLNV PPELYRLFRT KTWRDHVAAW DTIFNKAEKY TEIFYQDLRR KTEFRNYPGI
LYCLLKSEKM LLEDVKANIT EMLAGGVNTT SMTLQWHLYE MARSLNVQEM LREEVLNARR
QAEGDISKML QMVPLLKASI KETLRLHPIS VTLQRYPESD LVLQDYLIPA KTLVQVAIYA
MGRDPAFFSS PDKFDPTRWL SKDKDLIHFR NLGFGWGVRQ CVGRRIAELE MTLFLIHILE
NFKVEMQHIG DVDTIFNLIL TPDKPIFLVF RPFNQDPPQA


Related products :

Catalog number Product name Quantity
E0987p ELISA kit Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Pig,Sus scrofa 96T
U0987p CLIA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Pig,Sus scrofa 96T
E0987p ELISA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Pig,Sus scrofa 96T
E0987b ELISA Bos taurus,Bovine,Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc) 96T
U0987b CLIA Bos taurus,Bovine,Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc) 96T
E0987b ELISA kit Bos taurus,Bovine,Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc) 96T
E0987m ELISA kit Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,Cyp11a,Cyp11a1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Mouse,Mus musculus 96T
E0987m ELISA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,Cyp11a,Cyp11a1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Mouse,Mus musculus 96T
U0987Rb CLIA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Oryctolagus cuniculus,Rabbit 96T
E0987Rb ELISA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Oryctolagus cuniculus,Rabbit 96T
U0987m CLIA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,Cyp11a,Cyp11a1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Mouse,Mus musculus 96T
E0987Rb ELISA kit Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Oryctolagus cuniculus,Rabbit 96T
E0987h ELISA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Homo sapiens,Human 96T
U0987h CLIA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Homo sapiens,Human 96T
E0987h ELISA kit Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,CYP11A,CYP11A1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Homo sapiens,Human 96T
U0987r CLIA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,Cyp11a,Cyp11a1,Cyp11a-1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Rat,Rattus norvegicus 96T
E0987r ELISA Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,Cyp11a,Cyp11a1,Cyp11a-1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Rat,Rattus norvegicus 96T
E0987r ELISA kit Cholesterol desmolase,Cholesterol side-chain cleavage enzyme, mitochondrial,Cyp11a,Cyp11a1,Cyp11a-1,CYPXIA1,Cytochrome P450 11A1,Cytochrome P450(scc),Rat,Rattus norvegicus 96T
Y105004 Cytochrome P450 Side Chain Cleavage Enzyme (P450scc) 100 ul
Y105004 Cytochrome P450 Side Chain Cleavage Enzyme (P450scc) Antibody 100 ul
12-1244 Polyclonal antibody Anti-Rat Cytochrome p450 Side Chain Cleavage Enzyme 50
CSB-E12060p Pig Cytochrome P450 Side Chain Cleavage Enzyme(P450scc) ELISA kit SpeciesPig 96T
CSB-E12060p Porcine Cytochrome P450 Side Chain Cleavage Enzyme(P450scc) ELISA Kit 96tests
CSB-E12060p Porcine Cytochrome P450 Side Chain Cleavage Enzyme(P450scc) ELISA Kit 96tests
CSB-E12060p Porcine Cytochrome P450 Side Chain Cleavage Enzyme(P450scc) ELISA Kit 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur