Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Cyanidin-3-O-glucoside 2-O-glucuronosyltransferase (BpUGAT) (EC 2.4.1.254) (UDP-glucuronic acid:anthocyanin glucuronosyltransferase)

 UGAT_BELPE              Reviewed;         438 AA.
Q5NTH0;
27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
01-FEB-2005, sequence version 1.
30-AUG-2017, entry version 33.
RecName: Full=Cyanidin-3-O-glucoside 2-O-glucuronosyltransferase;
Short=BpUGAT;
EC=2.4.1.254;
AltName: Full=UDP-glucuronic acid:anthocyanin glucuronosyltransferase;
Name=UGAT; Synonyms=UGT94B1;
Bellis perennis (Daisy).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; asterids; campanulids; Asterales; Asteraceae;
Asteroideae; Astereae; Astereae incertae sedis; Bellis.
NCBI_TaxID=41492;
[1]
NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 102-112; 384-393 AND
417-426, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES,
ENZYME REGULATION, SUBUNIT, TISSUE SPECIFICITY, AND DEVELOPMENTAL
STAGE.
PubMed=15509561; DOI=10.1074/jbc.M410537200;
Sawada S., Suzuki H., Ichimaida F., Yamaguchi M.A., Iwashita T.,
Fukui Y., Hemmi H., Nishino T., Nakayama T.;
"UDP-glucuronic acid:anthocyanin glucuronosyltransferase from red
daisy (Bellis perennis) flowers. Enzymology and phylogenetics of a
novel glucuronosyltransferase involved in flower pigment
biosynthesis.";
J. Biol. Chem. 280:899-906(2005).
[2]
FUNCTION, 3D-STRUCTURE MODELING, BIOPHYSICOCHEMICAL PROPERTIES, AND
MUTAGENESIS OF ARG-25; ASN-123; LEU-148; ASP-152; PRO-174 AND ILE-187.
PubMed=18829982; DOI=10.1104/pp.108.128256;
Osmani S.A., Bak S., Imberty A., Olsen C.E., Moeller B.L.;
"Catalytic key amino acids and UDP-sugar donor specificity of a plant
glucuronosyltransferase, UGT94B1: molecular modeling substantiated by
site-specific mutagenesis and biochemical analyses.";
Plant Physiol. 148:1295-1308(2008).
-!- FUNCTION: Involved in the production of glucuronosylated
anthocyanins that are the origin of the red coloration of flowers.
Can use cyanidin 3-O-6''-O-malonylglucoside, cyanidin 3-O-
glucoside and delphinidin 3-O-glucosideas substrates, but not
pelargonidin 3-O-glucoside, cyanidin 3-O-3'',6''-O-
dimalonylglucoside, pelargonidin 3,5-O-diglucoside, pelargonidin
3-O-6''-O-malonylglucoside-5-O-glucoside, quercetin 3-O-glucoside,
quercetin 3-O-6''-O-malonylglucoside, daidzin, genistin,7-O-6''-O-
malonylglucosides of daidzein and genistein, cyanidin, quercetin,
daidzein, genistein p-Nitrophenyl beta-D-glucopyranoside, beta-
estradiol, 17alpha-estradiol, 1-naphthol, 2-naphthol, 4-
methylumbelliferone, and p-nitrophenol. Highly specific for UDP-
glucuronate (UDP-GlcUA). Arg-25 is decisive with respect to UDP-
sugar specificity. {ECO:0000269|PubMed:15509561,
ECO:0000269|PubMed:18829982}.
-!- CATALYTIC ACTIVITY: UDP-alpha-D-glucuronate + cyanidin 3-O-beta-D-
glucoside = UDP + cyanidin 3-O-(2-O-beta-D-glucuronosyl)-beta-D-
glucoside. {ECO:0000269|PubMed:15509561}.
-!- ENZYME REGULATION: Inhibited by copper, mercury, UDP, UTP and
partially by calcium, cadmium, iron and UMP. Not affected by
cobalt, magnesium, manganese, zinc, nickel, tin, uridine, sadium
malonate and glucose. {ECO:0000269|PubMed:15509561}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=19 uM for cyanidin 3-O-6''-O-malonylglucoside
{ECO:0000269|PubMed:15509561, ECO:0000269|PubMed:18829982};
KM=101 uM for UDP-glucuronate {ECO:0000269|PubMed:15509561,
ECO:0000269|PubMed:18829982};
KM=85 uM for cyanidin 3-O-glucoside
{ECO:0000269|PubMed:15509561, ECO:0000269|PubMed:18829982};
KM=730 uM for UDP-glucose {ECO:0000269|PubMed:15509561,
ECO:0000269|PubMed:18829982};
pH dependence:
Optimum pH is 8.0. {ECO:0000269|PubMed:15509561,
ECO:0000269|PubMed:18829982};
Temperature dependence:
Optimum temperature is 35 degrees Celsius.
{ECO:0000269|PubMed:15509561, ECO:0000269|PubMed:18829982};
-!- SUBUNIT: Monomer. {ECO:0000269|PubMed:15509561}.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
-!- TISSUE SPECIFICITY: Expressed in petals. Not detected in sepals,
stems, leaves, tubular corollas and white petals.
{ECO:0000269|PubMed:15509561}.
-!- DEVELOPMENTAL STAGE: Peak of expression at stage 4 of flower
development. {ECO:0000269|PubMed:15509561}.
-!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
{ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; AB190262; BAD77944.1; -; mRNA.
ProteinModelPortal; Q5NTH0; -.
CAZy; GT1; Glycosyltransferase Family 1.
KEGG; ag:BAD77944; -.
KO; K12937; -.
BioCyc; MetaCyc:MONOMER-15934; -.
BRENDA; 2.4.1.254; 11567.
SABIO-RK; Q5NTH0; -.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0102160; F:cyanidin-3-O-glucoside 2-O-glucuronosyltransferase activity; IEA:UniProtKB-EC.
GO; GO:0008152; P:metabolic process; IEA:InterPro.
InterPro; IPR002213; UDP_glucos_trans.
InterPro; IPR035595; UDP_glycos_trans_CS.
PANTHER; PTHR11926; PTHR11926; 1.
Pfam; PF00201; UDPGT; 1.
PROSITE; PS00375; UDPGT; 1.
1: Evidence at protein level;
Cytoplasm; Direct protein sequencing; Glycosyltransferase;
Transferase.
CHAIN 1 438 Cyanidin-3-O-glucoside 2-O-
glucuronosyltransferase.
/FTId=PRO_0000412107.
REGION 316 318 UDP-sugar binding. {ECO:0000250}.
REGION 333 341 UDP-sugar binding. {ECO:0000250}.
REGION 355 358 UDP-sugar binding. {ECO:0000250}.
MUTAGEN 25 25 R->G: Decreased activity with UDP-GlcUA
as donor. Increased activity with UDP-Glc
as donor. {ECO:0000269|PubMed:18829982}.
MUTAGEN 25 25 R->K: Decreased activity with UDP-GlcUA
as donor and decreased affinity.
Increased activity with UDP-Glc as donor.
{ECO:0000269|PubMed:18829982}.
MUTAGEN 25 25 R->P: Decreased activity with UDP-GlcUA
as donor. {ECO:0000269|PubMed:18829982}.
MUTAGEN 25 25 R->S: Decreased activity with UDP-GlcUA
as donor but no changes of the affinity.
Increased activity with UDP-Glc as donor.
{ECO:0000269|PubMed:18829982}.
MUTAGEN 123 123 N->A: Strongly decreased activity.
{ECO:0000269|PubMed:18829982}.
MUTAGEN 148 148 L->A: Strongly decreased activity.
{ECO:0000269|PubMed:18829982}.
MUTAGEN 152 152 D->A: Loss of activity.
{ECO:0000269|PubMed:18829982}.
MUTAGEN 174 174 P->G: Strongly decreased activity.
{ECO:0000269|PubMed:18829982}.
MUTAGEN 187 187 I->A,S: Strongly decreased activity.
{ECO:0000269|PubMed:18829982}.
SEQUENCE 438 AA; 49645 MW; 9CAC3D1BC58471E7 CRC64;
MDSKIDSKTF RVVMLPWLAY SHISRFLVFA KRLTNHNFHI YICSSQTNMQ YLKNNLTSQY
SKSIQLIELN LPSSSELPLQ YHTTHGLPPH LTKTLSDDYQ KSGPDFETIL IKLNPHLVIY
DFNQLWAPEV ASTLHIPSIQ LLSGCVALYA LDAHLYTKPL DENLAKFPFP EIYPKNRDIP
KGGSKYIERF VDCMRRSCEI ILVRSTMELE GKYIDYLSKT LGKKVLPVGP LVQEASLLQD
DHIWIMKWLD KKEESSVVFV CFGSEYILSD NEIEDIAYGL ELSQVSFVWA IRAKTSALNG
FIDRVGDKGL VIDKWVPQAN ILSHSSTGGF ISHCGWSSTM ESIRYGVPII AMPMQFDQPY
NARLMETVGA GIEVGRDGEG RLKREEIAAV VRKVVVEDSG ESIREKAKEL GEIMKKNMEA
EVDGIVIENL VKLCEMNN


Related products :

Catalog number Product name Quantity
EIAAB45210 GNT1,Homo sapiens,Human,Phenol-metabolizing UDP-glucuronosyltransferase,UDP-glucuronosyltransferase 1-6,UDP-glucuronosyltransferase 1A6,UDP-glucuronosyltransferase 1-F,UDPGT 1-6,UGT1,UGT1*6,UGT1.6,UGT
EIAAB45218 GNT1,Homo sapiens,Human,lugP4,UDP-glucuronosyltransferase 1-9,UDP-glucuronosyltransferase 1A9,UDP-glucuronosyltransferase 1-I,UDPGT 1-9,UGT1,UGT1*9,UGT1.9,UGT1-09,UGT1A9,UGT1I,UGT-1I
EIAAB45199 GNT1,Homo sapiens,Human,UDP-glucuronosyltransferase 1-10,UDP-glucuronosyltransferase 1A10,UDP-glucuronosyltransferase 1-J,UDPGT 1-10,UGT1,UGT1*10,UGT1.10,UGT1-10,UGT1A10,UGT1J,UGT-1J
EIAAB45212 GNT1,Homo sapiens,Human,UDP-glucuronosyltransferase 1-7,UDP-glucuronosyltransferase 1A7,UDP-glucuronosyltransferase 1-G,UDPGT 1-7,UGT1,UGT1*7,UGT1.7,UGT1-07,UGT1A7,UGT1G,UGT-1G
EIAAB45215 GNT1,Homo sapiens,Human,UDP-glucuronosyltransferase 1-8,UDP-glucuronosyltransferase 1A8,UDP-glucuronosyltransferase 1-H,UDPGT 1-8,UGT1,UGT1*8,UGT1.8,UGT1-08,UGT1A8,UGT1H,UGT-1H
EIAAB45207 GNT1,Homo sapiens,Human,UDP-glucuronosyltransferase 1-5,UDP-glucuronosyltransferase 1A5,UDP-glucuronosyltransferase 1-E,UDPGT 1-5,UGT1,UGT1*5,UGT1.5,UGT1-05,UGT1A5,UGT1E,UGT-1E
EIAAB45203 GNT1,Homo sapiens,Human,UDP-glucuronosyltransferase 1-3,UDP-glucuronosyltransferase 1A3,UDP-glucuronosyltransferase 1-C,UDPGT 1-3,UGT1,UGT1*3,UGT1.3,UGT1-03,UGT1A3,UGT1C,UGT-1C
EIAAB45196 Bilirubin-specific UDPGT isozyme 1,GNT1,Homo sapiens,hUG-BR1,Human,UDP-glucuronosyltransferase 1-1,UDP-glucuronosyltransferase 1-A,UDP-glucuronosyltransferase 1A1,UDPGT 1-1,UGT1,UGT1*1,UGT1.1,UGT1-01,
EIAAB45204 Bilirubin-specific UDPGT isozyme 2,GNT1,Homo sapiens,hUG-BR2,Human,UDP-glucuronosyltransferase 1-4,UDP-glucuronosyltransferase 1A4,UDP-glucuronosyltransferase 1-D,UDPGT 1-4,UGT1,UGT1*4,UGT1.4,UGT1-04,
orb61863 Cyanidin 3-O-glucoside Cyanidin 3-O-glucoside For research use only. 20 mg
EIAAB45245 M-1,Mouse,Mus musculus,UDP-glucuronosyltransferase 2B17,UDP-glucuronosyltransferase 2B5,UDPGT 2B5,Ugt2b17,Ugt2b5
EIAAB45229 3,4-catechol estrogen-specific UDPGT,Homo sapiens,Human,UDP-glucuronosyltransferase 2B7,UDP-glucuronosyltransferase 2B9,UDPGT 2B7,UDPGT 2B9,UDPGTh-2,UGT2B7,UGTB2B9
EIAAB45240 HLUG4,Homo sapiens,Human,UDP-glucuronosyltransferase 2B15,UDP-glucuronosyltransferase 2B8,UDPGT 2B15,UDPGT 2B8,UDPGTh-3,UGT2B15,UGT2B8
EIAAB45197 Mouse,Mus musculus,UDP-glucuronosyltransferase 1-1,UDP-glucuronosyltransferase 1A1,UDPGT 1-1,Ugt1,UGT1*1,UGT1.1,UGT1-01,Ugt1a1,UGTBR1
EIAAB45248 17-beta-hydroxysteroid-specific UDPGT,Rat,Rattus norvegicus,UDP-glucuronosyltransferase 2B37,UDP-glucuronosyltransferase R-21,UDPGT 2B37,UDPGTr-21,UDPGTr-5,Ugt2b37,Ugt2b5,Ugt2b6
EIAAB45243 17-beta-hydroxysteroid-specific UDPGT,Rat,Rattus norvegicus,RLUG38,Testosterone, dihydrotestosterone, and beta-estradiol-specific UDPGT,UDP-glucuronosyltransferase 2B17,UDP-glucuronosyltransferase 2B5
EIAAB45198 B1,Rat,Rattus norvegicus,UDP-glucuronosyltransferase 1-1,UDP-glucuronosyltransferase 1A1,UDPGT 1-1,Ugt1,UGT1*1,UGT1.1,UGT1-01,Ugt1a1
EIAAB45241 Rat,Rattus norvegicus,UDP-glucuronosyltransferase 2B15,UDP-glucuronosyltransferase 2B36,UDPGT 2B15,UDPGT 2B36,Ugt2b12,Ugt2b15,Ugt2b36,Ugt2b4
EIAAB45208 Mouse,Mus musculus,Phenol UDP-glucuronosyltransferase,UDP-glucuronosyltransferase 1-6,UDPGT 1-6,UGP1A1,Ugt1,UGT1*6,UGT1.6,UGT1-06,UGT1A6,Ugt1a6,Ugt1a6a
BP0430 Cyanidin-3-glucoside chloride; Chrysanthemin 20mg
BP0430 Cyanidin-3-glucoside chloride; Chrysanthemin 100mg
BP0430 Cyanidin-3-glucoside chloride; Chrysanthemin 1000mg
EIAAB45244 C19-steroid-specific UDP-glucuronosyltransferase,C19-steroid-specific UDPGT,Homo sapiens,Human,UDP-glucuronosyltransferase 2B17,UDPGT 2B17,UGT2B17
EIAAB45217 Mouse,Mus musculus,UDP-glucuronosyltransferase 1-7,UDP-glucuronosyltransferase 1-9,UDPGT,UDPGT 1-9,Ugt1,UGT1*9,UGT1.9,UGT1-09,UGT1A12,Ugt1a12,Ugt1a9,UGTP4
EIAAB45227 HLUG25,Homo sapiens,Human,Hyodeoxycholic acid-specific UDPGT,UDP-glucuronosyltransferase 2B4,UDPGT 2B4,UDPGTh-1,UGT2B11,UGT2B4


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur