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DASH complex subunit DAD2 (DUO1 and DAM1-interacting protein 2) (Helper of ASK1 protein 1) (Outer kinetochore protein DAD2)

 DAD2_YEAST              Reviewed;         133 AA.
P36162; D6VXE3;
01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
01-JUN-1994, sequence version 1.
18-JUL-2018, entry version 141.
RecName: Full=DASH complex subunit DAD2;
AltName: Full=DUO1 and DAM1-interacting protein 2;
AltName: Full=Helper of ASK1 protein 1;
AltName: Full=Outer kinetochore protein DAD2;
Name=DAD2; Synonyms=HSK1; OrderedLocusNames=YKR083C; ORFNames=YKR403;
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
NCBI_TaxID=559292;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
PubMed=8203164; DOI=10.1002/yea.320100210;
Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G.,
Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.;
"The complete sequence of an 18,002 bp segment of Saccharomyces
cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes,
and six new open reading frames.";
Yeast 10:231-245(1994).
[2]
GENOME REANNOTATION.
STRAIN=ATCC 204508 / S288c;
PubMed=24374639; DOI=10.1534/g3.113.008995;
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
Cherry J.M.;
"The reference genome sequence of Saccharomyces cerevisiae: Then and
now.";
G3 (Bethesda) 4:389-398(2014).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=8196765; DOI=10.1038/369371a0;
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M.,
Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C.,
Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G.,
Zimmermann J., Haasemann M., Becker I., Mewes H.-W.;
"Complete DNA sequence of yeast chromosome XI.";
Nature 369:371-378(1994).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=17322287; DOI=10.1101/gr.6037607;
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-
encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[5]
COMPONENT OF DASH COMPLEX.
PubMed=11799062; DOI=10.1101/gad.959402;
Li Y., Bachant J.B., Alcasabas A.A., Wang Y., Qin J., Elledge S.J.;
"The mitotic spindle is required for loading of the DASH complex onto
the kinetochore.";
Genes Dev. 16:183-197(2002).
[6]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
PubMed=14562095; DOI=10.1038/nature02026;
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
Weissman J.S., O'Shea E.K.;
"Global analysis of protein localization in budding yeast.";
Nature 425:686-691(2003).
[7]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
PubMed=14562106; DOI=10.1038/nature02046;
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[8]
FUNCTION.
PubMed=15664196; DOI=10.1016/j.molcel.2004.12.019;
Westermann S., Avila-Sakar A., Wang H.-W., Niederstrasser H., Wong J.,
Drubin D.G., Nogales E., Barnes G.;
"Formation of a dynamic kinetochore-microtubule interface through
assembly of the Dam1 ring complex.";
Mol. Cell 17:277-290(2005).
[9]
ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY
MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
PubMed=22814378; DOI=10.1073/pnas.1210303109;
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
Aldabe R.;
"N-terminal acetylome analyses and functional insights of the N-
terminal acetyltransferase NatB.";
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
[10]
ELECTRON MICROSCOPY OF DASH COMPLEX ALONE AND BOUND TO MICROTUBULES.
PubMed=15640796; DOI=10.1038/nsmb896;
Miranda J.L., Wulf P.D., Sorger P.K., Harrison S.C.;
"The yeast DASH complex forms closed rings on microtubules.";
Nat. Struct. Mol. Biol. 12:138-143(2005).
[11]
FUNCTION.
PubMed=16415853; DOI=10.1038/nature04409;
Westermann S., Wang H.-W., Avila-Sakar A., Drubin D.G., Nogales E.,
Barnes G.;
"The Dam1 kinetochore ring complex moves processively on
depolymerizing microtubule ends.";
Nature 440:565-569(2006).
[12]
SUBUNIT.
PubMed=16715078; DOI=10.1038/ncb1414;
Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D.;
"Molecular architecture of a kinetochore-microtubule attachment
site.";
Nat. Cell Biol. 8:581-585(2006).
[13]
FUNCTION.
PubMed=16777964; DOI=10.1073/pnas.0602249103;
Asbury C.L., Gestaut D.R., Powers A.F., Franck A.D., Davis T.N.;
"The Dam1 kinetochore complex harnesses microtubule dynamics to
produce force and movement.";
Proc. Natl. Acad. Sci. U.S.A. 103:9873-9878(2006).
-!- FUNCTION: Component of the DASH complex, a microtubule-binding
subcomplex of the outer kinetochore that is essential for proper
chromosome segregation. The DASH complex mediates the formation
and maintenance of bipolar kinetochore-microtubule attachments by
forming closed rings around spindle microtubules and establishing
interactions with proteins from the central kinetochore. The DASH
ring complex may both stabilize microtubules during chromosome
attachment in anaphase A, and allow the chromosome to remain
attached to the depolymerizing microtubule in anaphase B.
Microtubule depolymerization proceeds by protofilament splaying
and induces the kinetochore-attached ring to slide longitudinally,
thereby helping to transduce depolymerization energy into pulling
forces to disjoin chromatids. {ECO:0000269|PubMed:15664196,
ECO:0000269|PubMed:16415853, ECO:0000269|PubMed:16777964}.
-!- SUBUNIT: The DASH complex is an approximately 210 kDa
heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4,
DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry
of one copy of each subunit. DASH oligomerizes into a 50 nm ring
composed of about 16 molecules that encircles the microtubule.
Integrity of the complex and interactions with central kinetochore
proteins are regulated by the spindle assembly checkpoint kinase
IPL1. {ECO:0000269|PubMed:16715078}.
-!- INTERACTION:
P35734:ASK1; NbExp=9; IntAct=EBI-26515, EBI-26682;
P53267:DAM1; NbExp=2; IntAct=EBI-26515, EBI-23268;
P53168:DUO1; NbExp=2; IntAct=EBI-26515, EBI-23800;
Q03954:SPC19; NbExp=3; IntAct=EBI-26515, EBI-38809;
-!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:14562095}.
Chromosome, centromere, kinetochore {ECO:0000269|PubMed:14562095}.
Note=Associates with the mitotic spindle and the kinetochore.
-!- MISCELLANEOUS: Present with 279 molecules/cell in log phase SD
medium. {ECO:0000269|PubMed:14562106}.
-!- SIMILARITY: Belongs to the DASH complex DAD2 family.
{ECO:0000305}.
-----------------------------------------------------------------------
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EMBL; Z27116; CAA81634.1; -; Genomic_DNA.
EMBL; Z28308; CAA82162.1; -; Genomic_DNA.
EMBL; AY558397; AAS56723.1; -; Genomic_DNA.
EMBL; BK006944; DAA09233.1; -; Genomic_DNA.
PIR; S38161; S38161.
RefSeq; NP_013009.1; NM_001179873.1.
ProteinModelPortal; P36162; -.
BioGrid; 34214; 216.
ComplexPortal; CPX-1041; DASH complex.
DIP; DIP-1927N; -.
IntAct; P36162; 16.
MINT; P36162; -.
STRING; 4932.YKR083C; -.
iPTMnet; P36162; -.
MaxQB; P36162; -.
PaxDb; P36162; -.
PRIDE; P36162; -.
EnsemblFungi; YKR083C; YKR083C; YKR083C.
GeneID; 853958; -.
KEGG; sce:YKR083C; -.
EuPathDB; FungiDB:YKR083C; -.
SGD; S000001791; DAD2.
HOGENOM; HOG000001096; -.
InParanoid; P36162; -.
KO; K11567; -.
OMA; LQYTEND; -.
OrthoDB; EOG092C59JG; -.
BioCyc; YEAST:G3O-32046-MONOMER; -.
PRO; PR:P36162; -.
Proteomes; UP000002311; Chromosome XI.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
GO; GO:0042729; C:DASH complex; IDA:SGD.
GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
GO; GO:1990023; C:mitotic spindle midzone; IBA:GO_Central.
GO; GO:0044732; C:mitotic spindle pole body; IBA:GO_Central.
GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; IDA:SGD.
GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO; GO:1990758; P:mitotic sister chromatid biorientation; IBA:GO_Central.
GO; GO:0051987; P:positive regulation of attachment of spindle microtubules to kinetochore; IDA:SGD.
GO; GO:0031116; P:positive regulation of microtubule polymerization; IDA:SGD.
InterPro; IPR013963; DASH_Dad2.
PANTHER; PTHR28036; PTHR28036; 1.
Pfam; PF08654; DASH_Dad2; 1.
1: Evidence at protein level;
Acetylation; Cell cycle; Cell division; Centromere; Chromosome;
Chromosome partition; Coiled coil; Complete proteome; Cytoplasm;
Cytoskeleton; Kinetochore; Microtubule; Mitosis; Nucleus;
Reference proteome.
CHAIN 1 133 DASH complex subunit DAD2.
/FTId=PRO_0000211598.
COILED 1 51 {ECO:0000255}.
MOD_RES 1 1 N-acetylmethionine.
{ECO:0000244|PubMed:22814378}.
SEQUENCE 133 AA; 15072 MW; 82730D4AA5B66951 CRC64;
MDSIDEQIAI KRKELQSLQK ITSLTDGLKI QLTELNEQIK EMGMNADSVA QLMNNWDSII
NNISQASLGL LQYAEGDYEI GPWKDSKKKE SEQSNETGLE AQENDKNDED NDEDEDLVPL
PETMVRIRVD GNE


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