Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

DNA damage checkpoint control protein MEC3

 MEC3_YEAST              Reviewed;         474 AA.
Q02574; D6VYT4; P87260; Q05887;
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
13-JUN-2006, sequence version 3.
31-JAN-2018, entry version 134.
RecName: Full=DNA damage checkpoint control protein MEC3;
Name=MEC3; Synonyms=PIP3, PSO9; OrderedLocusNames=YLR288C;
ORFNames=L8003.15;
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
NCBI_TaxID=559292;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=7491494; DOI=10.1126/science.270.5241.1488;
Lydall D., Weinert T.A.;
"Yeast checkpoint genes in DNA damage processing: implications for
repair and arrest.";
Science 270:1488-1491(1995).
[2]
SEQUENCE REVISION TO 27; 411; 415-222 AND 454.
Lydall D., Weinert T.A.;
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169871;
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B.,
Ansorge W., Benes V., Brueckner M., Delius H., Dubois E.,
Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U.,
Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K.,
Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T.,
Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E.,
Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M.,
Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C.,
Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M.,
Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H.,
Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A.,
Hani J., Hoheisel J.D.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
Nature 387:87-90(1997).
[4]
GENOME REANNOTATION.
STRAIN=ATCC 204508 / S288c;
PubMed=24374639; DOI=10.1534/g3.113.008995;
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
Cherry J.M.;
"The reference genome sequence of Saccharomyces cerevisiae: Then and
now.";
G3 (Bethesda) 4:389-398(2014).
[5]
INTERACTION WITH DDC1.
PubMed=9670034; DOI=10.1093/emboj/17.14.4199;
Paciotti V., Lucchini G., Plevani P., Longhese M.P.;
"Mec1p is essential for phosphorylation of the yeast DNA damage
checkpoint protein Ddc1p, which physically interacts with Mec3p.";
EMBO J. 17:4199-4209(1998).
[6]
IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, AND FUNCTION OF THE
CHECKPOINT CLAMP COMPLEX.
PubMed=9891048; DOI=10.1128/MCB.19.2.1136;
Kondo T., Matsumoto K., Sugimoto K.;
"Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA
damage checkpoint pathway.";
Mol. Cell. Biol. 19:1136-1143(1999).
[7]
FUNCTION, AND INTERACTION WITH SET1.
PubMed=9988274; DOI=10.1038/5991;
Corda Y., Schramke V., Longhese M.P., Smokvina T., Paciotti V.,
Brevet V., Gilson E., Geli V.;
"Interaction between Set1p and checkpoint protein Mec3p in DNA repair
and telomere functions.";
Nat. Genet. 21:204-208(1999).
[8]
FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
PubMed=12271137; DOI=10.1073/pnas.202463999;
Giannattasio M., Sommariva E., Vercillo R., Lippi-Boncambi F.,
Liberi G., Foiani M., Plevani P., Muzi-Falconi M.;
"A dominant-negative MEC3 mutant uncovers new functions for the Rad17
complex and Tel1.";
Proc. Natl. Acad. Sci. U.S.A. 99:12997-13002(2002).
[9]
IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, AND FUNCTION OF THE
CHECKPOINT CLAMP COMPLEX.
PubMed=12672803; DOI=10.1074/jbc.M301260200;
Giannattasio M., Sabbioneda S., Minuzzo M., Plevani P.,
Muzi-Falconi M.;
"Correlation between checkpoint activation and in vivo assembly of the
yeast checkpoint complex Rad17-Mec3-Ddc1.";
J. Biol. Chem. 278:22303-22308(2003).
[10]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
PubMed=14562106; DOI=10.1038/nature02046;
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[11]
INTERACTION OF THE CHECKPOINT CLAMP COMPLEX WITH THE RFC-RAD24
CHECKPOINT CLAMP LOADER COMPLEX, AND FUNCTION OF THE CHECKPOINT CLAMP
COMPLEX.
PubMed=12604797; DOI=10.1073/pnas.0437148100;
Majka J., Burgers P.M.J.;
"Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage
checkpoint.";
Proc. Natl. Acad. Sci. U.S.A. 100:2249-2254(2003).
[12]
INTERACTION OF THE CHECKPOINT CLAMP COMPLEX WITH THE RFC-RAD24
CHECKPOINT CLAMP LOADER COMPLEX.
PubMed=15014082; DOI=10.1074/jbc.M400898200;
Majka J., Chung B.Y., Burgers P.M.J.;
"Requirement for ATP by the DNA damage checkpoint clamp loader.";
J. Biol. Chem. 279:20921-20926(2004).
[13]
IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, AND FUNCTION OF THE
CHECKPOINT CLAMP COMPLEX.
PubMed=16137930; DOI=10.1016/j.dnarep.2005.07.008;
Majka J., Burgers P.M.J.;
"Function of Rad17/Mec3/Ddc1 and its partial complexes in the DNA
damage checkpoint.";
DNA Repair 4:1189-1194(2005).
[14]
INTERACTION WITH REV7, AND FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
PubMed=16169844; DOI=10.1074/jbc.M507638200;
Sabbioneda S., Minesinger B.K., Giannattasio M., Plevani P.,
Muzi-Falconi M., Jinks-Robertson S.;
"The 9-1-1 checkpoint clamp physically interacts with polzeta and is
partially required for spontaneous polzeta-dependent mutagenesis in
Saccharomyces cerevisiae.";
J. Biol. Chem. 280:38657-38665(2005).
[15]
FUNCTION, AND INTERACTION WITH DDC1 AND RAD17.
PubMed=16202664; DOI=10.1016/j.dnarep.2005.08.018;
Cardone J.M., Revers L.F., Machado R.M., Bonatto D., Brendel M.,
Henriques J.A.P.;
"Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains
a mutant allele of the DNA damage checkpoint gene MEC3.";
DNA Repair 5:163-171(2006).
[16]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-452, AND IDENTIFICATION
BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth
phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
-!- FUNCTION: Component of the checkpoint clamp complex involved in
the surveillance mechanism that allows the DNA repair pathways to
act to restore the integrity of the DNA prior to DNA synthesis or
separation of the replicated chromosomes. Associates with sites of
DNA damage and modulates the MEC1 signaling pathway and the
activation of RAD53 in response to DNA damage at phase G1. The
complex also physically regulates DNA polymerase zeta-dependent
mutagenesis by controlling the access of polymerase zeta to
damaged DNA. {ECO:0000269|PubMed:12271137,
ECO:0000269|PubMed:12604797, ECO:0000269|PubMed:12672803,
ECO:0000269|PubMed:16137930, ECO:0000269|PubMed:16169844,
ECO:0000269|PubMed:16202664, ECO:0000269|PubMed:9891048,
ECO:0000269|PubMed:9988274}.
-!- SUBUNIT: Component of the checkpoint clamp complex composed of
DDC1, MEC3 and RAD17. The interaction with MEC3 is performed in a
RAD17-dependent manner. The checkpoint clamp complex loads onto
DNA. Interacts with the DNA polymerase zeta subunit REV7. Forms
also a heterotrimer with 2 RAD17 subunits. Interacts with SET1.
{ECO:0000269|PubMed:12604797, ECO:0000269|PubMed:12672803,
ECO:0000269|PubMed:15014082, ECO:0000269|PubMed:16137930,
ECO:0000269|PubMed:16169844, ECO:0000269|PubMed:16202664,
ECO:0000269|PubMed:9670034, ECO:0000269|PubMed:9891048,
ECO:0000269|PubMed:9988274}.
-!- INTERACTION:
Q08949:DDC1; NbExp=5; IntAct=EBI-10658, EBI-30769;
P48581:RAD17; NbExp=11; IntAct=EBI-10658, EBI-14652;
P38927:REV7; NbExp=3; IntAct=EBI-10658, EBI-14960;
P38827:SET1; NbExp=3; IntAct=EBI-10658, EBI-16977;
-!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
-!- MISCELLANEOUS: Present with 414 molecules/cell in log phase SD
medium. {ECO:0000269|PubMed:14562106}.
-!- SIMILARITY: Belongs to the MEC3 family. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; U30906; AAB61448.1; -; Genomic_DNA.
EMBL; U17243; AAB67334.1; -; Genomic_DNA.
EMBL; BK006945; DAA09600.1; -; Genomic_DNA.
PIR; S50373; S50373.
RefSeq; NP_013391.1; NM_001182176.1.
ProteinModelPortal; Q02574; -.
BioGrid; 31554; 147.
DIP; DIP-1432N; -.
IntAct; Q02574; 26.
MINT; MINT-406637; -.
STRING; 4932.YLR288C; -.
iPTMnet; Q02574; -.
MaxQB; Q02574; -.
PaxDb; Q02574; -.
PRIDE; Q02574; -.
EnsemblFungi; YLR288C; YLR288C; YLR288C.
GeneID; 850995; -.
KEGG; sce:YLR288C; -.
EuPathDB; FungiDB:YLR288C; -.
SGD; S000004279; MEC3.
HOGENOM; HOG000113516; -.
InParanoid; Q02574; -.
KO; K02544; -.
OMA; EPQVWCK; -.
OrthoDB; EOG092C49MG; -.
BioCyc; YEAST:G3O-32383-MONOMER; -.
PRO; PR:Q02574; -.
Proteomes; UP000002311; Chromosome XII.
GO; GO:0030896; C:checkpoint clamp complex; IDA:SGD.
GO; GO:0000781; C:chromosome, telomeric region; IEA:GOC.
GO; GO:0035861; C:site of double-strand break; IBA:GO_Central.
GO; GO:0003677; F:DNA binding; IDA:SGD.
GO; GO:0006348; P:chromatin silencing at telomere; IMP:SGD.
GO; GO:0000077; P:DNA damage checkpoint; IMP:SGD.
GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
GO; GO:0031573; P:intra-S DNA damage checkpoint; IBA:GO_Central.
GO; GO:0044778; P:meiotic DNA integrity checkpoint; IBA:GO_Central.
GO; GO:0033314; P:mitotic DNA replication checkpoint; IBA:GO_Central.
GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
GO; GO:0000722; P:telomere maintenance via recombination; IGI:SGD.
InterPro; IPR007150; Hus1/Mec3.
Pfam; PF04005; Hus1; 1.
1: Evidence at protein level;
Cell cycle; Complete proteome; DNA damage; DNA repair; DNA-binding;
Nucleus; Phosphoprotein; Reference proteome.
CHAIN 1 474 DNA damage checkpoint control protein
MEC3.
/FTId=PRO_0000096344.
MOD_RES 452 452 Phosphoserine.
{ECO:0000244|PubMed:18407956}.
CONFLICT 120 120 T -> I (in Ref. 1; AAB61448).
{ECO:0000305}.
CONFLICT 176 176 N -> D (in Ref. 1; AAB61448).
{ECO:0000305}.
CONFLICT 454 454 D -> E (in Ref. 1; AAB61448).
{ECO:0000305}.
SEQUENCE 474 AA; 53146 MW; 4385CEFA9130FEA3 CRC64;
MKLKLIVNGC EAPDDYKLLR TTINTVASLR KTAILRFNSE RLTIISTPKS SLNSSNNGTI
LRGDTGQLWC TIPHDVFRLY TVISARELNT ITMECNCDSL LSVFKRYDRV MNQGSSSNMT
IKLQSMPEWN TNNGTLSGGT AGGVDTTSKP NPICALGITF EEIVHTSGPN DAIVMNGGVD
EHNGLPTTVG TGNLLASNKV IMHSFKVPVK LLFRAQDTRI QEPMINYIQL MMYKLPPISG
EFGSAFHGFI RRVERYSNVN HIHLMGVKKK EHGNEGDDVE LKIIVNELDW HLEICWNGPL
DSVIQRQEGL TDNPSQNQHI DTDGRQEEGS LPIIEADKPM SSLYTNTRDR EMEENIRYDE
DLLRIEDSSI ADTRGNIYTA DTSGDTEFND ISVMVEKAEQ ESSSTHEVII RCKDWKVCSK
LYAAFEEVVL AISHDESCVF HCSLDRGSLE DSEDVEKPRE RGQIIYYIAR SKGL


Related products :

Catalog number Product name Quantity
63-107 Anti_Cut5 (anti_Rad4) S. pombe Cut5_Rad4_Dre3 protein is an essential component for DNA replication and also for the damage and checkpoint control which couples S and M phases. It interacts with chrom 100 ul
EIAAB33643 Cell cycle checkpoint protein RAD1,DNA repair exonuclease rad1 homolog,Homo sapiens,hRAD1,Human,RAD1,Rad1-like DNA damage checkpoint protein,REC1
EIAAB33644 Cell cycle checkpoint protein RAD1,DNA repair exonuclease rad1 homolog,Mouse,mRAD1,Mus musculus,Rad1,Rad1-like DNA damage checkpoint protein,Rec1
27-724 Checkpoint suppressor 1 is a member of the forkhead_winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 s 0.1 mg
CSB-EL013616RA Rat Mediator of DNA damage checkpoint protein 1(MDC1) ELISA kit 96T
CSB-EL013616PI Pig Mediator of DNA damage checkpoint protein 1(MDC1) ELISA kit SpeciesPig 96T
CSB-EL013616HU Human Mediator of DNA damage checkpoint protein 1(MDC1) ELISA kit 96T
CSB-EL013616MO Mouse Mediator of DNA damage checkpoint protein 1(MDC1) ELISA kit 96T
CSB-EL013616RA Rat Mediator of DNA damage checkpoint protein 1(MDC1) ELISA kit SpeciesRat 96T
CSB-EL013616RH Monkey Mediator of DNA damage checkpoint protein 1(MDC1) ELISA kit SpeciesMonkey 96T
CSB-EL013616MO Mouse Mediator of DNA damage checkpoint protein 1(MDC1) ELISA kit SpeciesMouse 96T
CSB-EL013616HU Human Mediator of DNA damage checkpoint protein 1(MDC1) ELISA kit SpeciesHuman 96T
MDC1_MOUSE ELISA Kit FOR Mediator of DNA damage checkpoint protein 1; organism: Mouse; gene name: Mdc1 96T
18-783-76591 SHEEP ANTI HUMAN MDC1 (aa1883-2089) - MEDIATOR OF DNA DAMAGE CHECKPOINT PROTEIN 1; Polyclonal 0.1 ml
MDD1 MDC1 Gene mediator of DNA-damage checkpoint 1
27-305 In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor 0.05 mg
27-302 RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protei 0.1 mg
30-841 HUS1Bis most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. HUS1B can interact with the check point protein RA 0.05 mg
CSB-EL013616RA Rat mediator of DNA damage checkpoint 1 (MDC1) ELISA kit, Species Rat, Sample Type serum, plasma 96T
CSB-EL013616PI Pig mediator of DNA damage checkpoint 1 (MDC1) ELISA kit, Species Pig, Sample Type serum, plasma 96T
EIAAB10707 Damage-specific DNA-binding protein 1,DDB p127 subunit,DDB1,DDBa,DNA damage-binding protein 1,DNA damage-binding protein a,HBV X-associated protein 1,Homo sapiens,Human,UV-damaged DNA-binding factor,U
20-272-191366 Rad1 - Mouse monoclonal [4126] to Rad1; EC 3.1.11.2; DNA repair exonuclease rad1 homolog; Rad1-like DNA damage checkpoint protein; hRAD1 Monoclonal 0.2 mg
18-272-195603 Rad1 - Rabbit polyclonal to Rad1; EC 3.1.11.2; DNA repair exonuclease rad1 homolog; Rad1-like DNA damage checkpoint protein; hRAD1 Polyclonal 0.05 mg
EIAAB10733 Ddit4,Dexamethasone-induced gene 2 protein,Dig2,DNA damage-inducible transcript 4 protein,HIF-1 responsive protein RTP801,Mouse,Mus musculus,Protein regulated in development and DNA damage response 1,
CSB-EL013616RH Monkey mediator of DNA damage checkpoint 1 (MDC1) ELISA kit, Species Monkey, Sample Type serum, plasma 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur