Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

DNA repair protein RAD50 (EC 3.6.-.-) (153 kDa protein)

 RAD50_YEAST             Reviewed;        1312 AA.
P12753; D6W0U3;
01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
01-OCT-1989, sequence version 1.
05-JUL-2017, entry version 174.
RecName: Full=DNA repair protein RAD50;
EC=3.6.-.-;
AltName: Full=153 kDa protein;
Name=RAD50; OrderedLocusNames=YNL250W; ORFNames=N0872;
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
NCBI_TaxID=559292;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=RE821;
PubMed=2659437;
Alani E., Subbiah S., Kleckner N.;
"The yeast RAD50 gene encodes a predicted 153-kD protein containing a
purine nucleotide-binding domain and two large heptad-repeat
regions.";
Genetics 122:47-57(1989).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9234673;
DOI=10.1002/(SICI)1097-0061(199707)13:9<849::AID-YEA106>3.0.CO;2-N;
Sen-Gupta M., Gueldener U., Beinhauer J.D., Fiedler T.A.,
Hegemann J.H.;
"Sequence analysis of the 33 kb long region between ORC5 and SUI1 from
the left arm of chromosome XIV from Saccharomyces cerevisiae.";
Yeast 13:849-860(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169873;
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F.,
Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M.,
Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N.,
Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D.,
Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A.,
Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A.,
Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C.,
Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M.,
Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J.,
Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L.,
Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M.,
Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P.,
Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A.,
Wambutt R., Wedler H., Zollner A., Hani J.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV
and its evolutionary implications.";
Nature 387:93-98(1997).
[4]
GENOME REANNOTATION.
STRAIN=ATCC 204508 / S288c;
PubMed=24374639; DOI=10.1534/g3.113.008995;
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
Cherry J.M.;
"The reference genome sequence of Saccharomyces cerevisiae: Then and
now.";
G3 (Bethesda) 4:389-398(2014).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
PubMed=14562106; DOI=10.1038/nature02046;
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-469 AND THR-568, AND
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth
phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
-!- FUNCTION: Involved in DNA double-strand break repair (DSBR). The
rad50/mre11 complex possesses single-strand endonuclease activity
and ATP-dependent double-strand-specific exonuclease activity.
Rad50 provides ATP-dependent control of mre11 by unwinding and/or
repositioning DNA ends into the mre11 active site.
-!- COFACTOR:
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
Note=Binds 1 zinc ion per homodimer. {ECO:0000250};
-!- SUBUNIT: Homodimer (By similarity). Forms a complex with MRE11.
{ECO:0000250}.
-!- INTERACTION:
P32829:MRE11; NbExp=6; IntAct=EBI-14700, EBI-11255;
-!- DOMAIN: The zinc-hook, which separates the large intramolecular
coiled coil regions, contains 2 Cys residues that coordinate one
molecule of zinc with the help of the 2 Cys residues of the zinc-
hook of another RAD50 molecule, thereby forming a V-shaped
homodimer. {ECO:0000250}.
-!- MISCELLANEOUS: Present with 830 molecules/cell in log phase SD
medium. {ECO:0000269|PubMed:14562106}.
-!- SIMILARITY: Belongs to the SMC family. RAD50 subfamily.
{ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; X14814; CAA32919.1; -; Genomic_DNA.
EMBL; X96722; CAA65494.1; -; Genomic_DNA.
EMBL; Z71526; CAA96157.1; -; Genomic_DNA.
EMBL; BK006947; DAA10309.1; -; Genomic_DNA.
PIR; S05808; BWBYDL.
RefSeq; NP_014149.1; NM_001183088.1.
ProteinModelPortal; P12753; -.
SMR; P12753; -.
BioGrid; 35589; 325.
DIP; DIP-2419N; -.
IntAct; P12753; 17.
MINT; MINT-435300; -.
STRING; 4932.YNL250W; -.
iPTMnet; P12753; -.
MaxQB; P12753; -.
PRIDE; P12753; -.
EnsemblFungi; YNL250W; YNL250W; YNL250W.
GeneID; 855471; -.
KEGG; sce:YNL250W; -.
EuPathDB; FungiDB:YNL250W; -.
SGD; S000005194; RAD50.
GeneTree; ENSGT00390000018781; -.
HOGENOM; HOG000192269; -.
InParanoid; P12753; -.
KO; K10866; -.
OMA; RSMVCTQ; -.
OrthoDB; EOG092C0FLY; -.
BioCyc; YEAST:G3O-33247-MONOMER; -.
Reactome; R-SCE-2559586; DNA Damage/Telomere Stress Induced Senescence.
Reactome; R-SCE-5693548; Sensing of DNA Double Strand Breaks.
Reactome; R-SCE-5693565; Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
Reactome; R-SCE-912446; Meiotic recombination.
PRO; PR:P12753; -.
Proteomes; UP000002311; Chromosome XIV.
GO; GO:0000794; C:condensed nuclear chromosome; IBA:GO_Central.
GO; GO:0030870; C:Mre11 complex; IPI:SGD.
GO; GO:0000790; C:nuclear chromatin; IBA:GO_Central.
GO; GO:0000784; C:nuclear chromosome, telomeric region; IBA:GO_Central.
GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
GO; GO:0005634; C:nucleus; IDA:SGD.
GO; GO:0035861; C:site of double-strand break; IBA:GO_Central.
GO; GO:0004017; F:adenylate kinase activity; IDA:SGD.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0016887; F:ATPase activity; IDA:SGD.
GO; GO:0003690; F:double-stranded DNA binding; IDA:SGD.
GO; GO:0003691; F:double-stranded telomeric DNA binding; IDA:SGD.
GO; GO:0051880; F:G-quadruplex DNA binding; IDA:SGD.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0043047; F:single-stranded telomeric DNA binding; IDA:SGD.
GO; GO:0042162; F:telomeric DNA binding; IDA:SGD.
GO; GO:0006284; P:base-excision repair; IMP:SGD.
GO; GO:0070192; P:chromosome organization involved in meiotic cell cycle; IBA:GO_Central.
GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
GO; GO:0000727; P:double-strand break repair via break-induced replication; TAS:SGD.
GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IMP:SGD.
GO; GO:0035753; P:maintenance of DNA trinucleotide repeats; IMP:SGD.
GO; GO:0051321; P:meiotic cell cycle; IMP:SGD.
GO; GO:0042138; P:meiotic DNA double-strand break formation; TAS:SGD.
GO; GO:0000706; P:meiotic DNA double-strand break processing; TAS:SGD.
GO; GO:0097552; P:mitochondrial double-strand break repair via homologous recombination; IMP:SGD.
GO; GO:0032078; P:negative regulation of endodeoxyribonuclease activity; IDA:SGD.
GO; GO:0007131; P:reciprocal meiotic recombination; IBA:GO_Central.
GO; GO:0016233; P:telomere capping; IBA:GO_Central.
GO; GO:0000723; P:telomere maintenance; IMP:SGD.
GO; GO:0000722; P:telomere maintenance via recombination; IMP:SGD.
GO; GO:0007004; P:telomere maintenance via telomerase; IBA:GO_Central.
InterPro; IPR027417; P-loop_NTPase.
InterPro; IPR004584; Rad50_eukaryotes.
InterPro; IPR013134; Zn_hook_RAD50.
Pfam; PF04423; Rad50_zn_hook; 1.
SUPFAM; SSF52540; SSF52540; 3.
TIGRFAMs; TIGR00606; rad50; 1.
PROSITE; PS51131; ZN_HOOK; 1.
1: Evidence at protein level;
ATP-binding; Coiled coil; Complete proteome; DNA damage; DNA repair;
Hydrolase; Meiosis; Metal-binding; Nucleotide-binding; Phosphoprotein;
Reference proteome; Zinc.
CHAIN 1 1312 DNA repair protein RAD50.
/FTId=PRO_0000138648.
DOMAIN 640 741 Zinc-hook. {ECO:0000255|PROSITE-
ProRule:PRU00471}.
NP_BIND 34 41 ATP. {ECO:0000255}.
COILED 185 347 {ECO:0000255}.
COILED 403 558 {ECO:0000255}.
COILED 640 678 {ECO:0000255}.
COILED 712 741 {ECO:0000255}.
COILED 787 1108 {ECO:0000255}.
COMPBIAS 1204 1241 Ala/Asp-rich (DA-box).
METAL 687 687 Zinc. {ECO:0000255|PROSITE-
ProRule:PRU00471}.
METAL 690 690 Zinc. {ECO:0000255|PROSITE-
ProRule:PRU00471}.
MOD_RES 469 469 Phosphoserine.
{ECO:0000244|PubMed:18407956}.
MOD_RES 568 568 Phosphothreonine.
{ECO:0000244|PubMed:18407956}.
SEQUENCE 1312 AA; 152569 MW; 58A0AA173AC5677E CRC64;
MSAIYKLSIQ GIRSFDSNDR ETIEFGKPLT LIVGMNGSGK TTIIECLKYA TTGDLPPNSK
GGVFIHDPKI TGEKDIRAQV KLAFTSANGL NMIVTRNIQL LMKKTTTTFK TLEGQLVAIN
NSGDRSTLST RSLELDAQVP LYLGVPKAIL EYVIFCHQED SLWPLSEPSN LKKKFDEIFQ
AMKFTKALDN LKSIKKDMSV DIKLLKQSVE HLKLDKDRSK AMKLNIHQLQ TKIDQYNEEV
SEIESQLNEI TEKSDKLFKS NQDFQKILSK VENLKNTKLS ISDQVKRLSN SIDILDLSKP
DLQNLLANFS KVLMDKNNQL RDLETDISSL KDRQSSLQSL SNSLIRRQGE LEAGKETYEK
NRNHLSSLKE AFQHKFQGLS NIENSDMAQV NHEMSQFKAF ISQDLTDTID QFAKDIQLKE
TNLSDLIKSI TVDSQNLEYN KKDRSKLIHD SEELAEKLKS FKSLSTQDSL NHELENLKTY
KEKLQSWESE NIIPKLNQKI EEKNNEMIIL ENQIEKFQDR IMKTNQQADL YAKLGLIKKS
INTKLDELQK ITEKLQNDSR IRQVFPLTQE FQRADLEMDF QKLFINMQKN IAINNKKMHE
LDRRYTNALY NLNTIEKDLQ DNQKSKEKVI QLLSENLPED CTIDEYNDVL EETELSYKTA
LENLKMHQTT LEFNRKALEI AERDSCCYLC SRKFENESFK SKLLQELKTK TDANFEKTLK
DTVQNEKEYL HSLRLLEKHI ITLNSINEKI DNSQKCLEKA KEETKTSKSK LDELEVDSTK
LKDEKELAES EIRPLIEKFT YLEKELKDLE NSSKTISEEL SIYNTSEDGI QTVDELRDQQ
RKMNDSLREL RKTISDLQME KDEKVRENSR MINLIKEKEL TVSEIESSLT QKQNIDDSIR
SKRENINDID SRVKELEARI ISLKNKKDEA QSVLDKVKNE RDIQVRNKQK TVADINRLID
RFQTIYNEVV DFEAKGFDEL QTTIKELELN KAQMLELKEQ LDLKSNEVNE EKRKLADSNN
EEKNLKQNLE LIELKSQLQH IESEISRLDV QNAEAERDKY QEESLRLRTR FEKLSSENAG
KLGEMKQLQN QIDSLTHQLR TDYKDIEKNY HKEWVELQTR SFVTDDIDVY SKALDSAIMK
YHGLKMQDIN RIIDELWKRT YSGTDIDTIK IRSDEVSSTV KGKSYNYRVV MYKQDVELDM
RGRCSAGQKV LASIIIRLAL SETFGANCGV IALDEPTTNL DEENIESLAK SLHNIINMRR
HQKNFQLIVI THDEKFLGHM NAAAFTDHFF KVKRDDRQKS QIEWVDINRV TY


Related products :

Catalog number Product name Quantity
DL-RAD50-Hu Human DNA Repair Protein RAD50 (RAD50) ELISA Kit 96T
DL-RAD50-Mu Mouse DNA Repair Protein RAD50 (RAD50) ELISA Kit 96T
RAD50_MOUSE ELISA Kit FOR DNA repair protein RAD50; organism: Mouse; gene name: Rad50 96T
SEK111Hu ELISA Kit for DNA Repair Protein RAD50 (RAD50) Homo sapiens (Human) 96T
E99111Mu ELISA Kit for DNA Repair Protein RAD50 (RAD50) Organism: Mus musculus (Mouse) 96T
EIAAB33656 DNA repair protein RAD50,Homo sapiens,hRAD50,Human,RAD50
RAD50_HUMAN ELISA Kit FOR DNA repair protein RAD50; organism: Human; gene name: RAD50 96T
CSB-EL019262MO Mouse DNA repair protein RAD50(RAD50) ELISA kit SpeciesMouse 96T
EIAAB33654 DNA repair protein RAD50,Mouse,mRad50,Mus musculus,Rad50
SEK111Mu ELISA Kit for DNA Repair Protein RAD50 (RAD50) Mus musculus (Mouse) 96T
CSB-EL019262HU Human DNA repair protein RAD50(RAD50) ELISA kit SpeciesHuman 96T
EIAAB33655 DNA repair protein RAD50,Rad50,Rat,Rattus norvegicus
CSB-EL019262HU Human DNA repair protein RAD50(RAD50) ELISA kit 96T
CSB-EL019262MO Mouse DNA repair protein RAD50(RAD50) ELISA kit 96T
CSB-EL019262RA Rat DNA repair protein RAD50(RAD50) ELISA kit SpeciesRat 96T
201-02-1074 Mouse DNA Repair Protein RAD50(RAD50)ELISA Kit 96T
P99111Hu01 DNA Repair Protein RAD50 (RAD50) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P99111Hu01 DNA Repair Protein RAD50 (RAD50) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P99111Hu01 DNA Repair Protein RAD50 (RAD50) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
E99111Mu ELISA Kit for DNA Repair Protein RAD50 (RAD50), Organism Mus musculus (Mouse), Detection range 0.312-20ng_mL 96T/Kit
E99111Mu ELISA Kit for DNA Repair Protein RAD50 (RAD50) 96T/Kit
CSB-EL019262RA Rat DNA repair protein RAD50(RAD50) ELISA kit 96T
A99111Hu01 Antibody to DNA Repair Protein RAD50 (RAD50) Organism: Homo sapiens (Human) Type: Polyclonal Source: Rabbit 50ug
A99111Hu01 Antibody to DNA Repair Protein RAD50 (RAD50) Organism: Homo sapiens (Human) Type: Polyclonal Source: Rabbit 100ug
A99111Hu01-B Biotin-linked Antibody to DNA Repair Protein RAD50 (RAD50); Reactivity: Homo sapiens (Human) Clonality: Polyclonal Source: Rabbit 50ug


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur