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DNA replication ATP-dependent helicase/nuclease DNA2 (DNA replication ATP-dependent helicase-like homolog) [Includes: DNA replication nuclease DNA2 (EC 3.1.-.-); DNA replication ATP-dependent helicase DNA2 (EC 3.6.4.12)]

 DNA2_BOVIN              Reviewed;        1061 AA.
E1BMP7;
03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
03-OCT-2012, sequence version 3.
25-OCT-2017, entry version 49.
RecName: Full=DNA replication ATP-dependent helicase/nuclease DNA2;
AltName: Full=DNA replication ATP-dependent helicase-like homolog;
Includes:
RecName: Full=DNA replication nuclease DNA2;
EC=3.1.-.-;
Includes:
RecName: Full=DNA replication ATP-dependent helicase DNA2;
EC=3.6.4.12;
Name=DNA2;
Bos taurus (Bovine).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
Pecora; Bovidae; Bovinae; Bos.
NCBI_TaxID=9913;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Hereford;
PubMed=19393038; DOI=10.1186/gb-2009-10-4-r42;
Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C.,
Puiu D., Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S.,
Marcais G., Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.;
"A whole-genome assembly of the domestic cow, Bos taurus.";
Genome Biol. 10:R42.01-R42.10(2009).
-!- FUNCTION: Key enzyme involved in DNA replication and DNA repair in
nucleus and mitochondrion. Involved in Okazaki fragments
processing by cleaving long flaps that escape FEN1: flaps that are
longer than 27 nucleotides are coated by replication protein A
complex (RPA), leading to recruit DNA2 which cleaves the flap
until it is too short to bind RPA and becomes a substrate for
FEN1. Also involved in 5'-end resection of DNA during double-
strand break (DSB) repair: recruited by BLM and mediates the
cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by
the presence of RPA. Also involved in DNA replication checkpoint
independently of Okazaki fragments processing. Possesses different
enzymatic activities, such as single-stranded DNA (ssDNA)-
dependent ATPase, 5'-3' helicase and endonuclease activities.
While the ATPase and endonuclease activities are well-defined and
play a key role in Okazaki fragments processing and DSB repair,
the 5'-3' DNA helicase activity is subject to debate. According to
various reports, the helicase activity is weak and its function
remains largely unclear. Helicase activity may promote the motion
of DNA2 on the flap, helping the nuclease function (By
similarity). {ECO:0000250}.
-!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
-!- COFACTOR:
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
Evidence={ECO:0000250};
Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
-!- SUBUNIT: Interacts with BLM and WDHD1. {ECO:0000250}.
-!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Mitochondrion
{ECO:0000250}.
-!- PTM: Acetylated by EP300, leading to stimulate the 5'-3'
endonuclease, the 5'-3' helicase and DNA-dependent ATPase
activities, possibly by increasing DNA substrate affinity.
{ECO:0000250}.
-!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
{ECO:0000305}.
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EMBL; DAAA02061817; -; NOT_ANNOTATED_CDS; Genomic_DNA.
UniGene; Bt.62867; -.
SMR; E1BMP7; -.
STRING; 9913.ENSBTAP00000003395; -.
PaxDb; E1BMP7; -.
eggNOG; KOG1805; Eukaryota.
eggNOG; COG1112; LUCA.
InParanoid; E1BMP7; -.
Proteomes; UP000009136; Unplaced.
GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
GO; GO:0043139; F:5'-3' DNA helicase activity; ISS:UniProtKB.
GO; GO:0017108; F:5'-flap endonuclease activity; ISS:UniProtKB.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
GO; GO:0003678; F:DNA helicase activity; ISS:UniProtKB.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0004518; F:nuclease activity; ISS:UniProtKB.
GO; GO:0043142; F:single-stranded DNA-dependent ATPase activity; ISS:UniProtKB.
GO; GO:0000729; P:DNA double-strand break processing; ISS:UniProtKB.
GO; GO:0006260; P:DNA replication; ISS:UniProtKB.
GO; GO:0000076; P:DNA replication checkpoint; ISS:UniProtKB.
GO; GO:0033567; P:DNA replication, Okazaki fragment processing; ISS:UniProtKB.
InterPro; IPR026851; Dna2.
InterPro; IPR014808; DNA_replication_fac_Dna2_N.
InterPro; IPR027417; P-loop_NTPase.
PANTHER; PTHR10887:SF14; PTHR10887:SF14; 1.
Pfam; PF08696; Dna2; 1.
SUPFAM; SSF52540; SSF52540; 2.
3: Inferred from homology;
4Fe-4S; Acetylation; ATP-binding; Complete proteome; DNA damage;
DNA repair; DNA replication; DNA-binding; Endonuclease; Helicase;
Hydrolase; Iron; Iron-sulfur; Metal-binding; Mitochondrion;
Multifunctional enzyme; Nuclease; Nucleotide-binding; Nucleus;
Reference proteome.
CHAIN 1 1061 DNA replication ATP-dependent
helicase/nuclease DNA2.
/FTId=PRO_0000419465.
NP_BIND 648 655 ATP. {ECO:0000255}.
REGION 82 519 Nuclease activity. {ECO:0000250}.
REGION 520 1061 Helicase activity. {ECO:0000250}.
METAL 137 137 Iron-sulfur (4Fe-4S). {ECO:0000250}.
METAL 393 393 Iron-sulfur (4Fe-4S). {ECO:0000250}.
METAL 396 396 Iron-sulfur (4Fe-4S). {ECO:0000250}.
METAL 402 402 Iron-sulfur (4Fe-4S). {ECO:0000250}.
SEQUENCE 1061 AA; 120485 MW; DCF931ED3F1D3CBF CRC64;
MERVDELELL MEKSFWQEAE PSAELFQKKK VEASFSKIVL SRGMDNRYLV LAVDIVQSEE
GNHEKHLIIT ASQSLEYKEL CILRNDWCSV PVEPGDIIHL EGDCISNTWI IDEDFGYLIL
YPDMLISGTS IASSIRCMRR AVLSETFRSS DPATRQMLIG TVLHEVFQKA VSDSFAPEKL
QELASQTIQE IRHLKEMYRL KLNQDEIKQE VEEYLPSFSK WAGDFMHKHT STDFPQMQLS
LPSDGSNSNS TCNIEVTNSL DIEESIWSPR FGLKGKIDVT VGVKIHRGCK TKYKIMPLEL
KTGKESNSIE HRSQLVLYTL LSQERRADPE AGLLLYLKTG QMYPVPAKHL DKRELLRLRN
QMAFSLFHRI NKSTGEKTEL APLPQIIEEQ QTCKYCSQMG NCALYSRAVE QQMEDSSVPT
SMWPKIKEET QHLKPIHLEY FSLWCLMLTL ESQSKDNKRN YQHIWLMPAS EMEESGSCIG
SLIRIEHVKT VCDGQYLHNF QRKNGAIPIT NLMAGDRIIL SGEERTLFAL SRGYVKEINS
TTVTCSLDRN LSGLPESTLF RLDQEEKNCD IDTPLGNLSK LMENTRASQK LRDLIIDFRE
PQFISYLSSV LPHEAKDTVA CILKGLNKPQ RQAMKKVLLS KDYTLIVGMP GTGKTTTICT
LVRILYACGF SVLLTSYTHS AVDNILLKLA KFKIGFLRLG QIQKVHPDIQ KFTEEEICRS
KSIKSLALLE ELYNSQLIVA TTCMGINHPI FSRKTFDFCI VDEASQISQP VCLGPLFFSR
RFVLVGDHQQ LPPLVLNREA RALGMSESLF KRLEQNKNAV VQLTVQYRMN SKIMSLSNKL
TYEGKLECGS DKVANAVINL PNFKDVKLEL EFYADYSENP WLIAAFEPNN PVCFLNTHKV
PAPEQVEKGG VSNIMEAKLV VFLTSVFIKA GCKPSDIGII APYRQQLKVI SDLLAQSSVG
MVEVNTVDRY QGRDKSIVVV SFVRSNEDGT LGELLKDWRR LNVAITRAKH KLILLGCVPS
LSRYPPLRKL LNHLNSEKLI IDLPSGEHES LFHLLGDFQR K


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