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DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)

 A0A142CL76_9BACT        Unreviewed;       658 AA.
A0A142CL76;
08-JUN-2016, integrated into UniProtKB/TrEMBL.
08-JUN-2016, sequence version 1.
28-FEB-2018, entry version 11.
RecName: Full=DNA topoisomerase 1 {ECO:0000256|HAMAP-Rule:MF_00952};
EC=5.99.1.2 {ECO:0000256|HAMAP-Rule:MF_00952};
AltName: Full=DNA topoisomerase I {ECO:0000256|HAMAP-Rule:MF_00952};
Name=top {ECO:0000313|EMBL:AMQ11321.1};
Synonyms=topA {ECO:0000256|HAMAP-Rule:MF_00952};
ORFNames=pNA6-28 {ECO:0000313|EMBL:AMQ11321.1};
uncultured bacterium.
Plasmid pNA6 {ECO:0000313|EMBL:AMQ11321.1}.
Bacteria; environmental samples.
NCBI_TaxID=77133 {ECO:0000313|EMBL:AMQ11321.1};
[1] {ECO:0000313|EMBL:AMQ11321.1}
NUCLEOTIDE SEQUENCE.
PLASMID=pNA6 {ECO:0000313|EMBL:AMQ11321.1};
Dang B., Luo Y., Mao D.;
"Complete Nucleotide Sequence of pNA6, a 52,210-bp incU Plasmid, from
River Sediment.";
Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
-!- FUNCTION: Releases the supercoiling and torsional tension of DNA,
which is introduced during the DNA replication and transcription,
by transiently cleaving and rejoining one strand of the DNA
duplex. Introduces a single-strand break via transesterification
at a target site in duplex DNA. The scissile phosphodiester is
attacked by the catalytic tyrosine of the enzyme, resulting in the
formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the
expulsion of a 3'-OH DNA strand. The free DNA strand then
undergoes passage around the unbroken strand, thus removing DNA
supercoils. Finally, in the religation step, the DNA 3'-OH attacks
the covalent intermediate to expel the active-site tyrosine and
restore the DNA phosphodiester backbone. {ECO:0000256|HAMAP-
Rule:MF_00952}.
-!- CATALYTIC ACTIVITY: ATP-independent breakage of single-stranded
DNA, followed by passage and rejoining. {ECO:0000256|HAMAP-
Rule:MF_00952, ECO:0000256|SAAS:SAAS00721095}.
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Evidence={ECO:0000256|SAAS:SAAS00721088};
-!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00952,
ECO:0000256|SAAS:SAAS00709415}.
-!- SIMILARITY: Belongs to the type IA topoisomerase family.
{ECO:0000256|HAMAP-Rule:MF_00952, ECO:0000256|SAAS:SAAS00721110}.
-!- CAUTION: Lacks conserved residue(s) required for the propagation
of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00952}.
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EMBL; KU351673; AMQ11321.1; -; Genomic_DNA.
ProteinModelPortal; A0A142CL76; -.
GO; GO:0005694; C:chromosome; IEA:InterPro.
GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO; GO:0003917; F:DNA topoisomerase type I activity; IEA:UniProtKB-UniRule.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0006265; P:DNA topological change; IEA:UniProtKB-UniRule.
CDD; cd00186; TOP1Ac; 1.
CDD; cd03363; TOPRIM_TopoIA_TopoI; 1.
Gene3D; 1.10.290.10; -; 1.
Gene3D; 1.10.460.10; -; 2.
Gene3D; 2.70.20.10; -; 2.
HAMAP; MF_00952; Topoisom_1_prok; 1.
InterPro; IPR000380; Topo_IA.
InterPro; IPR003601; Topo_IA_2.
InterPro; IPR023406; Topo_IA_AS.
InterPro; IPR013497; Topo_IA_cen.
InterPro; IPR013824; Topo_IA_cen_sub1.
InterPro; IPR013825; Topo_IA_cen_sub2.
InterPro; IPR013826; Topo_IA_cen_sub3.
InterPro; IPR023405; Topo_IA_core_domain.
InterPro; IPR003602; Topo_IA_DNA-bd_dom.
InterPro; IPR013498; Topo_IA_Znf.
InterPro; IPR005733; TopoI_bac-type.
InterPro; IPR028612; Topoisom_1_IA.
InterPro; IPR006171; TOPRIM_domain.
InterPro; IPR034149; TOPRIM_TopoI.
PANTHER; PTHR42785; PTHR42785; 1.
Pfam; PF01131; Topoisom_bac; 1.
Pfam; PF01751; Toprim; 1.
Pfam; PF01396; zf-C4_Topoisom; 2.
PRINTS; PR00417; PRTPISMRASEI.
SMART; SM00437; TOP1Ac; 1.
SMART; SM00436; TOP1Bc; 1.
SMART; SM00493; TOPRIM; 1.
SUPFAM; SSF56712; SSF56712; 2.
TIGRFAMs; TIGR01051; topA_bact; 1.
PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
PROSITE; PS50880; TOPRIM; 1.
3: Inferred from homology;
DNA-binding {ECO:0000256|HAMAP-Rule:MF_00952,
ECO:0000256|SAAS:SAAS00721076};
Isomerase {ECO:0000256|HAMAP-Rule:MF_00952,
ECO:0000256|SAAS:SAAS00721067};
Magnesium {ECO:0000256|SAAS:SAAS00721141};
Metal-binding {ECO:0000256|SAAS:SAAS00721137};
Plasmid {ECO:0000313|EMBL:AMQ11321.1};
Topoisomerase {ECO:0000256|HAMAP-Rule:MF_00952,
ECO:0000256|SAAS:SAAS00721067}.
DOMAIN 1 110 Toprim. {ECO:0000259|PROSITE:PS50880}.
REGION 159 164 Interaction with DNA. {ECO:0000256|HAMAP-
Rule:MF_00952}.
ACT_SITE 282 282 O-(5'-phospho-DNA)-tyrosine intermediate.
{ECO:0000256|HAMAP-Rule:MF_00952}.
SITE 31 31 Interaction with DNA. {ECO:0000256|HAMAP-
Rule:MF_00952}.
SITE 134 134 Interaction with DNA. {ECO:0000256|HAMAP-
Rule:MF_00952}.
SITE 135 135 Interaction with DNA. {ECO:0000256|HAMAP-
Rule:MF_00952}.
SITE 138 138 Interaction with DNA. {ECO:0000256|HAMAP-
Rule:MF_00952}.
SITE 143 143 Interaction with DNA. {ECO:0000256|HAMAP-
Rule:MF_00952}.
SITE 284 284 Interaction with DNA. {ECO:0000256|HAMAP-
Rule:MF_00952}.
SITE 477 477 Interaction with DNA. {ECO:0000256|HAMAP-
Rule:MF_00952}.
SEQUENCE 658 AA; 72300 MW; 2459C352267AC95C CRC64;
MKLIIVESPG KVKKISEILG AGWRVAASVG HVRDLPEREM GVEAPDFKPK YVKTERGKEV
LAKLAGLASG AESVFLATDL DREGEAIAWH LQAALRLNNA QRVTFAEITP KAIKAAIMAP
RSVNIPMVAA QEGRRVLDRL VGYMVSPKLS DMSGRPGLSA GRVQSPAVRI IVEREKAIKN
FKPTAHFGVE LLFSGDWSAE WDLKPWLPDG TEYILDGQLA QRIAGIRSLQ VIGYEEKEAT
KAPPPPFITS TLQQAASVKL KMKPKATMAA AQKLYEQGHI TYMRTDSPNL SDDALIEIYQ
LCKAKGLPMV DRPRTWKAKG DAQEAHEAIR PSHFDLEEAG ETPEEKALYR LIWGRAVACQ
LAAARYAVRS VRLRATEPLD GRPVEFTAEG RTMTFSGWTG LFSDDTEDED DKDTKANNPI
PVLAPGQRLS AENGRVLNKK TRAPSRFTEA SLIAELERLG IGRPSTYAAI MDNIMRRQYV
EADKSGKYLV PTETGNLVIE ALVSGRFSFV EYDFTREMEG RLDLIAQGRL SYRDVVSAVH
GQLERELSGL KITAAPLHPC PDCGKALRRM KGKDGFFWGC TGYPDCSITL PDAAGKPGKP
KPKPQASEFA CPKCGKPLIR RKKAATGGKK AFDFFGCSGF PTCNEKFDVV GGKPKFTK


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