Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Dihydroxyacetone kinase (DHA kinase) (EC 2.7.1.29) (Glycerone kinase)

 DHAK_CITFR              Reviewed;         552 AA.
P45510;
01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
23-JAN-2007, sequence version 3.
23-MAY-2018, entry version 84.
RecName: Full=Dihydroxyacetone kinase;
Short=DHA kinase;
EC=2.7.1.29;
AltName: Full=Glycerone kinase;
Name=dhaK;
Citrobacter freundii.
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
NCBI_TaxID=546;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-14, SUBUNIT,
BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, AND CATALYTIC ACTIVITY.
STRAIN=ATCC 6750 / DSM 30040 / NCIB 8173 / M8BK;
PubMed=7635824; DOI=10.1128/jb.177.15.4392-4401.1995;
Daniel R., Stuertz K., Gottschalk G.;
"Biochemical and molecular characterization of the oxidative branch of
glycerol utilization by Citrobacter freundii.";
J. Bacteriol. 177:4392-4401(1995).
[2]
X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH ATP; MAGNESIUM
IONS; LIPID AND DIHYDROXYACETONE, ACTIVE SITE, CATALYTIC ACTIVITY,
SUBUNIT, AND MUTAGENESIS OF ASP-380; ASP-385; ASP-387 AND THR-388.
PubMed=12966101; DOI=10.1074/jbc.M305942200;
Siebold C., Arnold I., Garcia-Alles L.F., Baumann U., Erni B.;
"Crystal structure of the Citrobacter freundii dihydroxyacetone kinase
reveals an eight-stranded alpha-helical barrel ATP-binding domain.";
J. Biol. Chem. 278:48236-48244(2003).
-!- FUNCTION: Catalyzes the phosphorylation of dihydroxyacetone.
-!- CATALYTIC ACTIVITY: ATP + glycerone = ADP + glycerone phosphate.
{ECO:0000269|PubMed:12966101, ECO:0000269|PubMed:7635824}.
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Evidence={ECO:0000269|PubMed:7635824};
Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
Evidence={ECO:0000269|PubMed:7635824};
Note=Divalent metal cations, Mg(2+) or Ca(2+).
{ECO:0000269|PubMed:7635824};
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=30 uM for dihydroxyacetone {ECO:0000269|PubMed:7635824};
KM=70 uM for ATP {ECO:0000269|PubMed:7635824};
-!- PATHWAY: Polyol metabolism; glycerol fermentation; glycerone
phosphate from glycerol (oxidative route): step 2/2.
-!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12966101,
ECO:0000269|PubMed:7635824}.
-!- DOMAIN: The C-terminal domain consists of a eight-helix alpha
barrel. The eight helices form a pocket that includes a tightly
bound phospholipid. The cellular function of this lipid is
unknown; it has no significant effect on enzyme activity.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; U09771; AAB48843.1; -; Genomic_DNA.
PDB; 1UN8; X-ray; 2.50 A; A/B=1-552.
PDB; 1UN9; X-ray; 3.10 A; A/B=2-552.
PDBsum; 1UN8; -.
PDBsum; 1UN9; -.
ProteinModelPortal; P45510; -.
SMR; P45510; -.
DrugBank; DB01775; Dihydroxyacetone.
DrugBank; DB04395; Phosphoaminophosphonic Acid-Adenylate Ester.
PRIDE; P45510; -.
BioCyc; MetaCyc:MONOMER-4504; -.
BRENDA; 2.7.1.29; 1398.
SABIO-RK; P45510; -.
UniPathway; UPA00617; UER00669.
EvolutionaryTrace; P45510; -.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0004371; F:glycerone kinase activity; IEA:UniProtKB-EC.
GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0019588; P:anaerobic glycerol catabolic process; IEA:UniProtKB-UniPathway.
Gene3D; 1.25.40.340; -; 1.
InterPro; IPR012734; DhaK_ATP.
InterPro; IPR004006; DhaK_dom.
InterPro; IPR004007; DhaL_dom.
InterPro; IPR036117; DhaL_dom_sf.
Pfam; PF02733; Dak1; 1.
Pfam; PF02734; Dak2; 1.
SMART; SM01120; Dak2; 1.
SUPFAM; SSF101473; SSF101473; 1.
TIGRFAMs; TIGR02361; dak_ATP; 1.
PROSITE; PS51481; DHAK; 1.
PROSITE; PS51480; DHAL; 1.
1: Evidence at protein level;
3D-structure; ATP-binding; Calcium; Direct protein sequencing;
Glycerol metabolism; Kinase; Lipid-binding; Magnesium; Metal-binding;
Nucleotide-binding; Transferase.
INIT_MET 1 1 Removed. {ECO:0000269|PubMed:7635824}.
CHAIN 2 552 Dihydroxyacetone kinase.
/FTId=PRO_0000121528.
DOMAIN 8 327 DhaK. {ECO:0000255|PROSITE-
ProRule:PRU00814}.
DOMAIN 356 548 DhaL. {ECO:0000255|PROSITE-
ProRule:PRU00813}.
NP_BIND 385 388 ATP. {ECO:0000269|PubMed:12966101}.
NP_BIND 431 432 ATP. {ECO:0000269|PubMed:12966101}.
NP_BIND 476 477 ATP. {ECO:0000269|PubMed:12966101}.
NP_BIND 533 535 ATP. {ECO:0000269|PubMed:12966101}.
REGION 58 61 Substrate binding.
ACT_SITE 220 220 Tele-hemiaminal-histidine intermediate.
{ECO:0000255|PROSITE-ProRule:PRU00814,
ECO:0000269|PubMed:12966101}.
BINDING 109 109 Substrate.
BINDING 114 114 Substrate.
BINDING 468 468 ATP; via carbonyl oxygen.
{ECO:0000269|PubMed:12966101}.
MUTAGEN 380 380 D->A: Loss of kinase activity.
{ECO:0000269|PubMed:12966101}.
MUTAGEN 385 385 D->A: Loss of kinase activity.
{ECO:0000269|PubMed:12966101}.
MUTAGEN 387 387 D->A: Loss of kinase activity.
{ECO:0000269|PubMed:12966101}.
MUTAGEN 388 388 T->H: Reduced kinase activity.
{ECO:0000269|PubMed:12966101}.
HELIX 9 11 {ECO:0000244|PDB:1UN8}.
HELIX 12 22 {ECO:0000244|PDB:1UN8}.
STRAND 29 32 {ECO:0000244|PDB:1UN8}.
STRAND 40 44 {ECO:0000244|PDB:1UN8}.
STRAND 52 61 {ECO:0000244|PDB:1UN8}.
TURN 62 65 {ECO:0000244|PDB:1UN8}.
HELIX 66 68 {ECO:0000244|PDB:1UN8}.
STRAND 73 82 {ECO:0000244|PDB:1UN8}.
HELIX 88 98 {ECO:0000244|PDB:1UN8}.
STRAND 104 110 {ECO:0000244|PDB:1UN8}.
HELIX 112 127 {ECO:0000244|PDB:1UN8}.
STRAND 132 137 {ECO:0000244|PDB:1UN8}.
STRAND 146 148 {ECO:0000244|PDB:1UN9}.
HELIX 155 167 {ECO:0000244|PDB:1UN8}.
HELIX 172 184 {ECO:0000244|PDB:1UN8}.
STRAND 186 194 {ECO:0000244|PDB:1UN8}.
STRAND 203 205 {ECO:0000244|PDB:1UN8}.
STRAND 213 215 {ECO:0000244|PDB:1UN8}.
STRAND 225 229 {ECO:0000244|PDB:1UN8}.
HELIX 233 247 {ECO:0000244|PDB:1UN8}.
STRAND 254 260 {ECO:0000244|PDB:1UN8}.
HELIX 266 277 {ECO:0000244|PDB:1UN8}.
HELIX 282 284 {ECO:0000244|PDB:1UN8}.
STRAND 285 292 {ECO:0000244|PDB:1UN8}.
STRAND 300 309 {ECO:0000244|PDB:1UN8}.
HELIX 314 319 {ECO:0000244|PDB:1UN8}.
HELIX 356 371 {ECO:0000244|PDB:1UN8}.
HELIX 373 381 {ECO:0000244|PDB:1UN8}.
STRAND 384 386 {ECO:0000244|PDB:1UN9}.
HELIX 388 404 {ECO:0000244|PDB:1UN8}.
HELIX 413 427 {ECO:0000244|PDB:1UN8}.
HELIX 430 448 {ECO:0000244|PDB:1UN8}.
HELIX 453 468 {ECO:0000244|PDB:1UN8}.
STRAND 474 476 {ECO:0000244|PDB:1UN8}.
HELIX 478 490 {ECO:0000244|PDB:1UN8}.
HELIX 495 511 {ECO:0000244|PDB:1UN8}.
HELIX 529 531 {ECO:0000244|PDB:1UN8}.
HELIX 534 547 {ECO:0000244|PDB:1UN8}.
SEQUENCE 552 AA; 57940 MW; 8208307ECF3F79EA CRC64;
MSQFFFNQRT HLVSDVIDGA IIASPWNNLA RLESDPAIRI VVRRDLNKNN VAVISGGGSG
HEPAHVGFIG KGMLTAAVCG DVFASPSVDA VLTAIQAVTG EAGCLLIVKN YTGDRLNFGL
AAEKARRLGY NVEMLIVGDD ISLPDNKHPR GIAGTILVHK IAGYFAERGY NLATVLREAQ
YAASNTFSLG VALSSCHLPQ ETDAAPRHHP GHAELGMGIH GEPGASVIDT QNSAQVVNLM
VDKLLAALPE TGRLAVMINN LGGVSVAEMA IITRELASSP LHSRIDWLIG PASLVTALDM
KGFSLTAIVL EESIEKALLT EVETSNWPTP VPPREITCVV SSHASARVEF QPSANALVAG
IVELVTATLS DLETHLNALD AKVGDGDTGS TFAAAAREIA SLLHRQQLPL NNLATLFALI
GERLTVVMGG SSGVLMSIFF TAAGQKLEQG ANVVEALNTG LAQMKFYGGA DEGDRTMIDA
LQPALTSLLA QPKNLQAAFD AAQAGAERTC LSSKANAGRA SYLSSESLLG NMDPGAQRLA
MVFKALAESE LG


Related products :

Catalog number Product name Quantity
EIAAB24784 GCK family kinase MiNK,Map4k6,MAPK_ERK kinase kinase kinase 6,MEK kinase kinase 6,MEKKK 6,Mink,Mink1,Misshapen_NIK-related kinase,Misshapen-like kinase 1,Mitogen-activated protein kinase kinase kinase
EIAAB24783 GCK family kinase MiNK,Map4k6,MAPK_ERK kinase kinase kinase 6,MEK kinase kinase 6,MEKKK 6,Mink,Mink1,Misshapen_NIK-related kinase,Misshapen-like kinase 1,Mitogen-activated protein kinase kinase kinase
EIAAB25239 C-JUN N-terminal kinase kinase 1,Dual specificity mitogen-activated protein kinase kinase 4,JNK kinase 1,JNK-activating kinase 1,JNKK 1,Jnkk1,MAP kinase kinase 4,Map2k4,MAPK_ERK kinase 4,MAPKK 4,MEK 4
EIAAB25244 c-Jun N-terminal kinase kinase 2,Dual specificity mitogen-activated protein kinase kinase 7,JNK kinase 2,JNK-activating kinase 2,JNKK 2,MAP kinase kinase 7,Map2k7,MAPK_ERK kinase 7,MAPKK 7,MEK 7,Mkk7,
EIAAB25245 c-Jun N-terminal kinase kinase 2,Dual specificity mitogen-activated protein kinase kinase 7,JNK kinase 2,JNK-activating kinase 2,JNKK 2,MAP kinase kinase 7,Map2k7,MAPK_ERK kinase 7,MAPKK 7,MEK 7,Rat,R
EIAAB25246 c-Jun N-terminal kinase kinase 2,Dual specificity mitogen-activated protein kinase kinase 7,Homo sapiens,Human,JNK kinase 2,JNK-activating kinase 2,JNKK 2,JNKK2,MAP kinase kinase 7,MAP2K7,MAPK_ERK kin
EIAAB25240 c-Jun N-terminal kinase kinase 1,Dual specificity mitogen-activated protein kinase kinase 4,Homo sapiens,Human,JNK-activating kinase 1,JNKK,JNKK1,MAP kinase kinase 4,MAP2K4,MAPK_ERK kinase 4,MAPKK 4,M
E1358m ELISA kit Apoptosis signal-regulating kinase 1,Ask1,ASK-1,Map3k5,MAPK_ERK kinase kinase 5,MEK kinase 5,MEKK 5,Mekk5,Mitogen-activated protein kinase kinase kinase 5,Mouse,Mus musculus 96T
E1358m ELISA Apoptosis signal-regulating kinase 1,Ask1,ASK-1,Map3k5,MAPK_ERK kinase kinase 5,MEK kinase 5,MEKK 5,Mekk5,Mitogen-activated protein kinase kinase kinase 5,Mouse,Mus musculus 96T
U1358m CLIA Apoptosis signal-regulating kinase 1,Ask1,ASK-1,Map3k5,MAPK_ERK kinase kinase 5,MEK kinase 5,MEKK 5,Mekk5,Mitogen-activated protein kinase kinase kinase 5,Mouse,Mus musculus 96T
EIAAB24782 B55,GCK family kinase MiNK,Homo sapiens,Human,MAP4K6,MAPK_ERK kinase kinase kinase 6,MEK kinase kinase 6,MEKKK 6,MINK,MINK1,Misshapen_NIK-related kinase,Misshapen-like kinase 1,Mitogen-activated prote
U1358h CLIA Apoptosis signal-regulating kinase 1,ASK1,ASK-1,Homo sapiens,Human,MAP3K5,MAPK_ERK kinase kinase 5,MAPKKK5,MEK kinase 5,MEKK 5,MEKK5,Mitogen-activated protein kinase kinase kinase 5 96T
E1358h ELISA Apoptosis signal-regulating kinase 1,ASK1,ASK-1,Homo sapiens,Human,MAP3K5,MAPK_ERK kinase kinase 5,MAPKKK5,MEK kinase 5,MEKK 5,MEKK5,Mitogen-activated protein kinase kinase kinase 5 96T
E1358h ELISA kit Apoptosis signal-regulating kinase 1,ASK1,ASK-1,Homo sapiens,Human,MAP3K5,MAPK_ERK kinase kinase 5,MAPKKK5,MEK kinase 5,MEKK 5,MEKK5,Mitogen-activated protein kinase kinase kinase 5 96T
15-288-21068 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 Polyclonal 0.05 mg
18-662-20092 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 Polyclonal 0.1 ml
18-662-20091 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 Polyclonal 0.1 ml
18-662-20090 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 Polyclonal 0.1 ml
15-288-21068 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 Polyclonal 0.1 mg
10-782-55048 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 N_A 0.02 mg
18-785-210404 SEK1_MKK4 (Phospho-Ser80) - EC 2.7.12.2; MAP kinase kinase 4; JNK-activating kinase 1; c-Jun N-terminal kinase kinase 1; JNKK; SAPK_ERK kinase 1; SEK1 Polyclonal 0.1 mg
10-782-55048 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 N_A 0.05 mg
10-782-55047 Dual specificity mitogen-activated protein kinase kinase 1 - EC 2.7.12.2; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK_ERK kinase 1; MEK1 N_A 0.05 mg
18-785-210404 SEK1_MKK4 (Phospho-Ser80) - EC 2.7.12.2; MAP kinase kinase 4; JNK-activating kinase 1; c-Jun N-terminal kinase kinase 1; JNKK; SAPK_ERK kinase 1; SEK1 Polyclonal 0.05 mg
18-785-210403 SEK1_MKK4 (Phospho-Thr261) - EC 2.7.12.2; MAP kinase kinase 4; JNK-activating kinase 1; c-Jun N-terminal kinase kinase 1; JNKK; SAPK_ERK kinase 1; SEK1 Polyclonal 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur