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Endochitinase (EC 3.2.1.14) (Soluble cell wall protein 2)

 CHIT_YEAST              Reviewed;         562 AA.
P29029; D6VYT1; P29028;
01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
01-OCT-1996, sequence version 2.
25-OCT-2017, entry version 170.
RecName: Full=Endochitinase;
EC=3.2.1.14;
AltName: Full=Soluble cell wall protein 2;
Flags: Precursor;
Name=CTS1; Synonyms=SCW2; OrderedLocusNames=YLR286C;
ORFNames=L8003.13;
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
NCBI_TaxID=559292;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 21-38.
STRAIN=DBY918, and DBY939;
PubMed=1918080;
Kuranda M.J., Robbins P.W.;
"Chitinase is required for cell separation during growth of
Saccharomyces cerevisiae.";
J. Biol. Chem. 266:19758-19767(1991).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169871;
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B.,
Ansorge W., Benes V., Brueckner M., Delius H., Dubois E.,
Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U.,
Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K.,
Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T.,
Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E.,
Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M.,
Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C.,
Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M.,
Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H.,
Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A.,
Hani J., Hoheisel J.D.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
Nature 387:87-90(1997).
[3]
GENOME REANNOTATION.
STRAIN=ATCC 204508 / S288c;
PubMed=24374639; DOI=10.1534/g3.113.008995;
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
Cherry J.M.;
"The reference genome sequence of Saccharomyces cerevisiae: Then and
now.";
G3 (Bethesda) 4:389-398(2014).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=17322287; DOI=10.1101/gr.6037607;
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-
encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[5]
PROTEIN SEQUENCE OF 21-32, AND SUBCELLULAR LOCATION.
STRAIN=ATCC 96099 / S288c / SEY6210;
PubMed=9748433;
Cappellaro C., Mrsa V., Tanner W.;
"New potential cell wall glucanases of Saccharomyces cerevisiae and
their involvement in mating.";
J. Bacteriol. 180:5030-5037(1998).
[6]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
PubMed=14562106; DOI=10.1038/nature02046;
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
-!- FUNCTION: Chitinase is required for cell separation during growth
of Saccharomyces cerevisiae.
-!- CATALYTIC ACTIVITY: Random endo-hydrolysis of N-acetyl-beta-D-
glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
-!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9748433}.
Secreted, cell wall {ECO:0000269|PubMed:9748433}. Note=Most of the
enzyme is secreted, but a significant amount of chitinase is also
found associated with the cell wall through binding of C-terminal
domain to chitin.
-!- PTM: Extensively glycosylated with a series of short O-linked
mannose oligosaccharides ranging in size from Man(2) to Man(5).
-!- MISCELLANEOUS: Present with 6350 molecules/cell in log phase SD
medium. {ECO:0000269|PubMed:14562106}.
-!- SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase
class V subfamily. {ECO:0000305}.
-----------------------------------------------------------------------
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EMBL; M74070; AAA34539.1; -; Genomic_DNA.
EMBL; M74069; AAA34538.1; -; Genomic_DNA.
EMBL; U17243; AAB67331.1; -; Genomic_DNA.
EMBL; AY693040; AAT93059.1; -; Genomic_DNA.
EMBL; BK006945; DAA09597.1; -; Genomic_DNA.
PIR; A41035; A41035.
PIR; B41035; B41035.
PIR; S50371; S50371.
RefSeq; NP_013388.1; NM_001182173.1.
PDB; 2UY2; X-ray; 1.60 A; A=22-315.
PDB; 2UY3; X-ray; 1.90 A; A=22-315.
PDB; 2UY4; X-ray; 1.75 A; A=22-315.
PDB; 2UY5; X-ray; 1.60 A; A=22-315.
PDB; 4TXE; X-ray; 1.80 A; A=22-315.
PDBsum; 2UY2; -.
PDBsum; 2UY3; -.
PDBsum; 2UY4; -.
PDBsum; 2UY5; -.
PDBsum; 4TXE; -.
ProteinModelPortal; P29029; -.
SMR; P29029; -.
BioGrid; 31551; 79.
MINT; MINT-4495746; -.
STRING; 4932.YLR286C; -.
BindingDB; P29029; -.
ChEMBL; CHEMBL1293195; -.
CAZy; CBM19; Carbohydrate-Binding Module Family 19.
CAZy; GH18; Glycoside Hydrolase Family 18.
MaxQB; P29029; -.
PRIDE; P29029; -.
EnsemblFungi; YLR286C; YLR286C; YLR286C.
GeneID; 850992; -.
KEGG; sce:YLR286C; -.
EuPathDB; FungiDB:YLR286C; -.
SGD; S000004276; CTS1.
HOGENOM; HOG000160027; -.
InParanoid; P29029; -.
KO; K01183; -.
OMA; CDHSAWV; -.
OrthoDB; EOG092C49QH; -.
BioCyc; YEAST:YLR286C-MONOMER; -.
BRENDA; 3.2.1.14; 984.
EvolutionaryTrace; P29029; -.
PRO; PR:P29029; -.
Proteomes; UP000002311; Chromosome XII.
GO; GO:0005935; C:cellular bud neck; IDA:SGD.
GO; GO:0005576; C:extracellular region; IDA:SGD.
GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
GO; GO:0008061; F:chitin binding; IBA:GO_Central.
GO; GO:0008843; F:endochitinase activity; IDA:SGD.
GO; GO:0000920; P:cell separation after cytokinesis; IMP:SGD.
GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
GO; GO:0006032; P:chitin catabolic process; IBA:GO_Central.
GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
InterPro; IPR005089; CBM_fam19.
InterPro; IPR001223; Glyco_hydro18_cat.
InterPro; IPR001579; Glyco_hydro_18_chit_AS.
InterPro; IPR017853; Glycoside_hydrolase_SF.
Pfam; PF03427; CBM_19; 1.
Pfam; PF00704; Glyco_hydro_18; 1.
SUPFAM; SSF51445; SSF51445; 1.
PROSITE; PS01095; CHITINASE_18; 1.
1: Evidence at protein level;
3D-structure; Carbohydrate metabolism; Cell wall;
Cell wall biogenesis/degradation; Chitin degradation; Chitin-binding;
Complete proteome; Direct protein sequencing; Glycoprotein;
Glycosidase; Hydrolase; Polysaccharide degradation;
Reference proteome; Secreted; Signal.
SIGNAL 1 20 {ECO:0000269|PubMed:1918080,
ECO:0000269|PubMed:9748433}.
CHAIN 21 562 Endochitinase.
/FTId=PRO_0000011936.
REGION 21 327 Catalytic.
REGION 481 562 Chitin-binding, high affinity.
COMPBIAS 328 480 Ser/Thr-rich.
ACT_SITE 157 157 Proton donor. {ECO:0000255|PROSITE-
ProRule:PRU10053}.
CARBOHYD 553 553 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
VARIANT 16 16 L -> P (in strain: DBY939).
VARIANT 23 23 R -> S (in strain: DBY939 and SEY6210).
VARIANT 321 321 T -> S (in strain: DBY939).
VARIANT 336 340 Missing (in strain: DBY939).
VARIANT 399 399 S -> A (in strain: DBY939).
VARIANT 433 434 PI -> SL (in strain: DBY939).
VARIANT 461 461 T -> K (in strain: DBY939).
VARIANT 477 481 Missing (in strain: DBY939).
CONFLICT 168 168 A -> R (in Ref. 1; AAA34539/AAA34538).
{ECO:0000305}.
STRAND 28 35 {ECO:0000244|PDB:2UY2}.
HELIX 44 48 {ECO:0000244|PDB:2UY2}.
STRAND 50 52 {ECO:0000244|PDB:2UY2}.
STRAND 54 64 {ECO:0000244|PDB:2UY2}.
TURN 65 67 {ECO:0000244|PDB:2UY2}.
HELIX 72 74 {ECO:0000244|PDB:2UY2}.
HELIX 87 98 {ECO:0000244|PDB:2UY2}.
STRAND 102 108 {ECO:0000244|PDB:2UY2}.
STRAND 110 114 {ECO:0000244|PDB:2UY4}.
HELIX 119 133 {ECO:0000244|PDB:2UY2}.
TURN 143 146 {ECO:0000244|PDB:2UY2}.
STRAND 150 155 {ECO:0000244|PDB:2UY2}.
HELIX 164 175 {ECO:0000244|PDB:2UY2}.
STRAND 178 180 {ECO:0000244|PDB:2UY2}.
STRAND 183 186 {ECO:0000244|PDB:2UY2}.
STRAND 189 193 {ECO:0000244|PDB:2UY2}.
TURN 195 197 {ECO:0000244|PDB:2UY2}.
HELIX 198 203 {ECO:0000244|PDB:2UY2}.
STRAND 207 212 {ECO:0000244|PDB:2UY2}.
STRAND 214 216 {ECO:0000244|PDB:2UY2}.
STRAND 219 223 {ECO:0000244|PDB:2UY5}.
HELIX 226 235 {ECO:0000244|PDB:2UY2}.
STRAND 243 251 {ECO:0000244|PDB:2UY2}.
STRAND 254 257 {ECO:0000244|PDB:2UY2}.
HELIX 263 273 {ECO:0000244|PDB:2UY2}.
STRAND 279 285 {ECO:0000244|PDB:2UY2}.
HELIX 287 292 {ECO:0000244|PDB:2UY2}.
STRAND 294 299 {ECO:0000244|PDB:4TXE}.
HELIX 300 311 {ECO:0000244|PDB:2UY2}.
SEQUENCE 562 AA; 59015 MW; 0ABCEFBF448E1E19 CRC64;
MSLLYIILLF TQFLLLPTDA FDRSANTNIA VYWGQNSAGT QESLATYCES SDADIFLLSF
LNQFPTLGLN FANACSDTFS DGLLHCTQIA EDIETCQSLG KKVLLSLGGA SGSYLFSDDS
QAETFAQTLW DTFGEGTGAS ERPFDSAVVD GFDFDIENNN EVGYSALATK LRTLFAEGTK
QYYLSAAPQC PYPDASVGDL LENADIDFAF IQFYNNYCSV SGQFNWDTWL TYAQTVSPNK
NIKLFLGLPG SASAAGSGYI SDTSLLESTI ADIASSSSFG GIALWDASQA FSNELNGEPY
VEILKNLLTS ASQTATTTVA TSKTSAASTS SASTSSASTS QKKTTQSTTS TQSKSKVTLS
PTASSAIKTS ITQTTKTLTS STKTKSSLGT TTTESTLNSV AITSMKTTLS SQITSAALVT
PQTTTTSIVS SAPIQTAITS TLSPATKSSS VVSLQTATTS TLSPTTTSTS SGSTSSGSTS
SDSTARTLAK ELNAQYAAGK LNGKSTCTEG EIACSADGKF AVCDHSAWVY MECASGTTCY
AYDSGDSVYT QCNFSYLESN YF


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