Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Endochitinase A (EC 3.2.1.14) (Seed chitinase A)

 CHIA_MAIZE              Reviewed;         280 AA.
P29022;
01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
01-DEC-1992, sequence version 1.
23-MAY-2018, entry version 117.
RecName: Full=Endochitinase A {ECO:0000303|PubMed:1551872};
EC=3.2.1.14 {ECO:0000269|PubMed:1551872, ECO:0000269|PubMed:1740436};
AltName: Full=Seed chitinase A {ECO:0000303|PubMed:1551872};
Flags: Precursor;
Zea mays (Maize).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae;
PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae;
Zea.
NCBI_TaxID=4577;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
TISSUE=Seed;
PubMed=1551872;
Huynh Q.K., Hironaka C.M., Levine E.B., Smith C.E., Borgmeyer J.R.,
Shah D.M.;
"Antifungal proteins from plants. Purification, molecular cloning, and
antifungal properties of chitinases from maize seed.";
J. Biol. Chem. 267:6635-6640(1992).
[2]
PROTEIN SEQUENCE OF 180-195, ENZYME REGULATION, AND ACTIVE SITE.
TISSUE=Seed;
PubMed=1740436;
Verburg J.G., Smith C.E., Lisek C.A., Huynh Q.K.;
"Identification of an essential tyrosine residue in the catalytic site
of a chitinase isolated from Zea mays that is selectively modified
during inactivation with 1-ethyl-3-(3-dimethylaminopropyl)-
carbodiimide.";
J. Biol. Chem. 267:3886-3893(1992).
-!- FUNCTION: Defense against chitin-containing fungal pathogens.
{ECO:0000269|PubMed:1551872}.
-!- CATALYTIC ACTIVITY: Random endo-hydrolysis of N-acetyl-beta-D-
glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
{ECO:0000269|PubMed:1551872, ECO:0000269|PubMed:1740436}.
-!- ENZYME REGULATION: Inactivated by l-ethyl-3-(3-
dimethylaminopropyl)carbodiimide (EDC) in the absence of exogenous
nucleophiles (e.g. GlcNAc4, GlcNAc3 and GlcNAc2).
{ECO:0000269|PubMed:1740436}.
-!- MISCELLANEOUS: Maize chitinase B seems to be less active than
chitinase A. {ECO:0000269|PubMed:1551872}.
-!- SIMILARITY: Belongs to the glycosyl hydrolase 19 family. Chitinase
class I subfamily. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; M84164; AAA33444.1; -; Genomic_DNA.
PIR; A42424; A42424.
UniGene; Zm.119908; -.
UniGene; Zm.87300; -.
PDB; 4MCK; X-ray; 1.50 A; A=86-278.
PDBsum; 4MCK; -.
ProteinModelPortal; P29022; -.
SMR; P29022; -.
STRING; 4577.GRMZM2G051943_P01; -.
Allergome; 11981; Zea m 8.0101.
Allergome; 7663; Zea m 8.
CAZy; CBM18; Carbohydrate-Binding Module Family 18.
CAZy; GH19; Glycoside Hydrolase Family 19.
PaxDb; P29022; -.
PRIDE; P29022; -.
MaizeGDB; 25130; -.
eggNOG; KOG4742; Eukaryota.
eggNOG; COG3979; LUCA.
Proteomes; UP000007305; Unplaced.
ExpressionAtlas; P29022; differential.
GO; GO:0008061; F:chitin binding; IEA:UniProtKB-KW.
GO; GO:0004568; F:chitinase activity; IEA:UniProtKB-EC.
GO; GO:0016998; P:cell wall macromolecule catabolic process; IEA:InterPro.
GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW.
GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
CDD; cd00325; chitinase_glyco_hydro_19; 1.
Gene3D; 3.30.60.10; -; 1.
InterPro; IPR001002; Chitin-bd_1.
InterPro; IPR018371; Chitin-binding_1_CS.
InterPro; IPR036861; Endochitinase-like_sf.
InterPro; IPR016283; Glyco_hydro_19.
InterPro; IPR000726; Glyco_hydro_19_cat.
InterPro; IPR023346; Lysozyme-like_dom_sf.
Pfam; PF00187; Chitin_bind_1; 1.
Pfam; PF00182; Glyco_hydro_19; 2.
PIRSF; PIRSF001060; Endochitinase; 1.
ProDom; PD000609; Chitin_bd_1; 1.
SMART; SM00270; ChtBD1; 1.
SUPFAM; SSF53955; SSF53955; 1.
SUPFAM; SSF57016; SSF57016; 1.
PROSITE; PS00026; CHIT_BIND_I_1; 1.
PROSITE; PS50941; CHIT_BIND_I_2; 1.
PROSITE; PS00773; CHITINASE_19_1; 1.
PROSITE; PS00774; CHITINASE_19_2; 1.
1: Evidence at protein level;
3D-structure; Carbohydrate metabolism; Chitin degradation;
Chitin-binding; Complete proteome; Direct protein sequencing;
Disulfide bond; Glycosidase; Hydrolase; Plant defense;
Polysaccharide degradation; Reference proteome; Signal.
SIGNAL 1 25 {ECO:0000255}.
CHAIN 26 280 Endochitinase A.
/FTId=PRO_0000005303.
DOMAIN 26 60 Chitin-binding type-1.
{ECO:0000255|PROSITE-ProRule:PRU00261}.
REGION 61 77 Hinge region (poly-Gly). {ECO:0000305}.
REGION 78 280 Catalytic. {ECO:0000305}.
ACT_SITE 188 188 {ECO:0000269|PubMed:1740436}.
DISULFID 28 36 {ECO:0000255|PROSITE-ProRule:PRU00261}.
DISULFID 30 42 {ECO:0000255|PROSITE-ProRule:PRU00261}.
DISULFID 35 49 {ECO:0000255|PROSITE-ProRule:PRU00261}.
DISULFID 53 58 {ECO:0000255|PROSITE-ProRule:PRU00261}.
DISULFID 100 149 {ECO:0000255|PROSITE-ProRule:PRU00261}.
DISULFID 161 170 {ECO:0000255|PROSITE-ProRule:PRU00261}.
DISULFID 248 280 {ECO:0000255|PROSITE-ProRule:PRU00261}.
HELIX 86 93 {ECO:0000244|PDB:4MCK}.
TURN 101 105 {ECO:0000244|PDB:4MCK}.
HELIX 108 115 {ECO:0000244|PDB:4MCK}.
TURN 119 122 {ECO:0000244|PDB:4MCK}.
HELIX 127 145 {ECO:0000244|PDB:4MCK}.
TURN 146 149 {ECO:0000244|PDB:4MCK}.
STRAND 164 166 {ECO:0000244|PDB:4MCK}.
TURN 180 183 {ECO:0000244|PDB:4MCK}.
HELIX 187 197 {ECO:0000244|PDB:4MCK}.
TURN 201 203 {ECO:0000244|PDB:4MCK}.
HELIX 207 210 {ECO:0000244|PDB:4MCK}.
HELIX 212 226 {ECO:0000244|PDB:4MCK}.
HELIX 228 233 {ECO:0000244|PDB:4MCK}.
HELIX 235 242 {ECO:0000244|PDB:4MCK}.
STRAND 246 248 {ECO:0000244|PDB:4MCK}.
HELIX 253 269 {ECO:0000244|PDB:4MCK}.
SEQUENCE 280 AA; 29125 MW; 4FC5BB7D938C1CC1 CRC64;
MANAPRILAL GLLALLCAAA GPAAAQNCGC QPNFCCSKFG YCGTTDAYCG DGCQSGPCRS
GGGGGGGGGG GGGGSGGANV ANVVTDAFFN GIKNQAGSGC EGKNFYTRSA FLSAVNAYPG
FAHGGTEVEG KREIAAFFAH VTHETGHFCY ISEINKSNAY CDASNRQWPC AAGQKYYGRG
PLQISWNYNY GPAGRDIGFN GLADPNRVAQ DAVIAFKTAL WFWMNNVHGV MPQGFGATIR
AINGALECNG NNPAQMNARV GYYKQYCQQL RVDPGPNLIC


Related products :

Catalog number Product name Quantity
B1246 Polymyxin Seed Agar (Antibiotic Assay Medium No. 10) USE As a seed layer medium for assaying the products containing Polymyxin_B, also for assaying Carbenicillin, Colistin and Colistimethate sodium Qty per Litre of Medium: 52
B1246 Polymyxin Seed Agar (Antibiotic Assay Medium No. 10) USE As a seed layer medium for assaying the products containing Polymyxin_B, also for assaying Carbenicillin, Colistin and Colistimethate sodium Qty per Litre of Medium: 52
BS009 Antibiotic Assay Medium No.10 (Polymyxin Seed Agar) USE As a seed layer medium for assaying the products containing polymyxin_B. Also for assaying Carbenicillin,Colistin and Colistimethate sodium . Qty per Litre of Medium: 52
enz-031 Recombinant Clostridium Paraputrificum Chitinase Chitinase 100
enz-031 Recombinant Clostridium Paraputrificum Chitinase Chitinase 1mg
enz-031 Recombinant Clostridium Paraputrificum Chitinase Chitinase 20
TM 021 POLYMYXIN SEED AGAR (ANTIBIOTIC ASSAY MEDIUM NO.10) (Seed layer medium for assay of Poly 100 gm
TM 021 POLYMYXIN SEED AGAR (ANTIBIOTIC ASSAY MEDIUM NO.10) (Seed layer medium for assay of Poly 100 gm
TM 021 POLYMYXIN SEED AGAR (ANTIBIOTIC ASSAY MEDIUM NO.10) (Seed layer medium for assay of Poly 500 gm
TM 021 POLYMYXIN SEED AGAR (ANTIBIOTIC ASSAY MEDIUM NO.10) (Seed layer medium for assay of Poly 500 gm
BS009 Antibiotic Assay Medium No.10 (Polymyxin Seed Agar) USE : As a seed layer medium for assaying the products containing polymyxin-B. Also for assaying Carbenicillin,Colistin and Colistimethate sodium . 5x500gm
TM 021 ANTIBIOTIC ASSAY MEDIUM NO. 10 (POLYMYXIN SEED AGAR) (Seed layer medium for assay of 100 gm
TM 021 ANTIBIOTIC ASSAY MEDIUM NO. 10 (POLYMYXIN SEED AGAR) (Seed layer medium for assay of 500 gm
TM 021 ANTIBIOTIC ASSAY MEDIUM NO. 10 (POLYMYXIN SEED AGAR) (Seed layer medium for assay of 500 gm
TM 021 ANTIBIOTIC ASSAY MEDIUM NO. 10 (POLYMYXIN SEED AGAR) (Seed layer medium for assay of 100 gm
EIAAB07164 CHID1,Chitinase domain-containing protein 1,GL008,Homo sapiens,Human,PSEC0104,SB139,SI-CLP,Stabilin-1-interacting chitinase-like protein
AS07 207 Antibody: PR-3 | CHN | class I chitinase, Immunogen: purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990; van Buuren et al., 1992): 1) C 2 mg
E1463b ELISA 39 kDa whey protein,Bos taurus,Bovine,Cartilage glycoprotein 39,CGP-39,CHI3L1,Chitinase-3-like protein 1,Chitinase-like protein 1,CLP-1,GP-39,Signal-processing protein,SPC-40 96T
U1463b CLIA 39 kDa whey protein,Bos taurus,Bovine,Cartilage glycoprotein 39,CGP-39,CHI3L1,Chitinase-3-like protein 1,Chitinase-like protein 1,CLP-1,GP-39,Signal-processing protein,SPC-40 96T
E1463b ELISA kit 39 kDa whey protein,Bos taurus,Bovine,Cartilage glycoprotein 39,CGP-39,CHI3L1,Chitinase-3-like protein 1,Chitinase-like protein 1,CLP-1,GP-39,Signal-processing protein,SPC-40 96T
8016-06-06 Dill seed oil Dill seed oil 1g
A3500-100 quickAmp™ Seed PCR Kit, 100rxn_vial per vial
O-06483/98 OIL OF FENNEL SEED (Ex-Pure) 100 ml
O-06483/98 OIL OF FENNEL SEED (Ex-Pure) CAS: 500 ml
A3500 quickAmp™ Seed Genomic PCR Kit 100rxn/vial


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur