Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Endoribonuclease antitoxin GhoS (EC 3.1.-.-) (Antitoxin GhoS)

 GHOS_ECOLI              Reviewed;          98 AA.
P0AF61; P39275; Q2M6H6;
20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
20-DEC-2005, sequence version 1.
05-JUL-2017, entry version 72.
RecName: Full=Endoribonuclease antitoxin GhoS {ECO:0000303|PubMed:22941047};
EC=3.1.-.-;
AltName: Full=Antitoxin GhoS;
Name=ghoS {ECO:0000303|PubMed:22941047};
Synonyms=arT {ECO:0000303|PubMed:24797297}, yjdK;
OrderedLocusNames=b4128, JW4089;
Escherichia coli (strain K12).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=83333;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=7610040; DOI=10.1093/nar/23.12.2105;
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.,
Blattner F.R.;
"Analysis of the Escherichia coli genome VI: DNA sequence of the
region from 92.8 through 100 minutes.";
Nucleic Acids Res. 23:2105-2119(1995).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=9278503; DOI=10.1126/science.277.5331.1453;
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1462(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=16738553; DOI=10.1038/msb4100049;
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains
MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[4]
INDUCTION.
STRAIN=K12 / BW25113;
PubMed=23289863; DOI=10.1111/1462-2920.12063;
Wang X., Lord D.M., Hong S.H., Peti W., Benedik M.J., Page R.,
Wood T.K.;
"Type II toxin/antitoxin MqsR/MqsA controls type V toxin/antitoxin
GhoT/GhoS.";
Environ. Microbiol. 15:1734-1744(2013).
[5]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=24373067; DOI=10.1111/1462-2920.12373;
Cheng H.Y., Soo V.W., Islam S., McAnulty M.J., Benedik M.J.,
Wood T.K.;
"Toxin GhoT of the GhoT/GhoS toxin/antitoxin system damages the cell
membrane to reduce adenosine triphosphate and to reduce growth under
stress.";
Environ. Microbiol. 16:1741-1754(2014).
[6]
MUTAGENESIS OF LEU-25 AND MET-31.
STRAIN=K12 / BW25113;
PubMed=24797297; DOI=10.1038/srep04807;
Soo V.W., Cheng H.Y., Kwan B.W., Wood T.K.;
"de novo synthesis of a bacterial toxin/antitoxin system.";
Sci. Rep. 4:4807-4807(2014).
[7]
STRUCTURE BY NMR, FUNCTION, SUBUNIT, PROTEIN STABILITY, DISRUPTION
PHENOTYPE, AND MUTAGENESIS OF PHE-14; ASP-15; ARG-26; ARG-28 AND
PHE-55.
STRAIN=K12 / BW25113;
PubMed=22941047; DOI=10.1038/nchembio.1062;
Wang X., Lord D.M., Cheng H.Y., Osbourne D.O., Hong S.H.,
Sanchez-Torres V., Quiroga C., Zheng K., Herrmann T., Peti W.,
Benedik M.J., Page R., Wood T.K.;
"A new type V toxin-antitoxin system where mRNA for toxin GhoT is
cleaved by antitoxin GhoS.";
Nat. Chem. Biol. 8:855-861(2012).
-!- FUNCTION: Antitoxin component of a type V toxin-antitoxin (TA)
module. Neutralizes the toxic effects of toxin GhoT by digesting
ghoT transcripts in a sequence-specific manner (PubMed:22941047).
In concert with GhoT is involved in reducing cell growth during
antibacterial stress (PubMed:24373067). Overexpression leads to
transcript level reduction of 20 other mRNAs involved in purine or
pyrimidine synthesis and transport. Not seen to bind its own
promoter DNA (PubMed:22941047). {ECO:0000269|PubMed:22941047,
ECO:0000269|PubMed:24373067}.
-!- SUBUNIT: Monomer. {ECO:0000269|PubMed:22941047}.
-!- INDUCTION: Post-transcriptionally down-regulated by MqsR which
acts on the ghoST transcript selectively, degrading the ghoS
segment while leaving ghoT intact; conditions which induce MqsR
(e.g. overexpression, nalidixic acid, azolocillin or H(2)O(2))
decrease ghoS expression and thus increase ghoT transcripts
(PubMed:23289863). {ECO:0000269|PubMed:23289863}.
-!- PTM: Unlike other TA proteinaceous antitoxins, this protein is
stable with and without cellular stress; its structure has been
determined in the absence of GhoT toxin.
{ECO:0000269|PubMed:22941047}.
-!- DISRUPTION PHENOTYPE: Essential; cannot be disrupted unless ghoT
is also disrupted; in the double ghoS-ghoT mutant has no visible
phenotype (PubMed:24373067, PubMed:22941047). Increased biofilm
formation after 8 hours at 30 and 37 degrees Celsius, has risen
higher by 24 hours at 37 degrees Celsius but has fallen by 24
hours at 30 degrees Celsius. Approximately 2-fold increase in
swimming motility (PubMed:24373067). When single ghoT mutant is
grown in the presence of antibiotics carbenicillin or cefoxitin
initial metabolism is significantly increased over that of wild-
type, after 14 hours wild-type is slighlty less active. In a
double ghoS-ghoT mutant in presence of the 2 antibiotics
metabolism is significantly increased over that of wild-type, but
by 9 hours wild-type has caught up and eventually has slightly
greater metablbic rates (PubMed:24373067).
{ECO:0000269|PubMed:22941047, ECO:0000269|PubMed:24373067}.
-!- MISCELLANEOUS: Has a similar 3D-structure to Cas2 proteins.
{ECO:0000269|PubMed:22941047}.
-!- MISCELLANEOUS: Can be modified to become a classic type II TA
toxin (called ArT) which causes cells to become ghost-like,
probably by broadening substrate RNA recognition; the toxic
activity is independent of ghoT and mqsRA. Antitoxins to this
evolved ArT toxin have been artifically evolved from antitoxins
mqsA (a type II TA system) and toxI (a type I TA system).
{ECO:0000269|PubMed:24797297}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; U14003; AAA97028.1; -; Genomic_DNA.
EMBL; U00096; AAC77089.1; -; Genomic_DNA.
EMBL; AP009048; BAE78130.1; -; Genomic_DNA.
PIR; S56357; S56357.
RefSeq; NP_418552.1; NC_000913.3.
RefSeq; WP_000398619.1; NZ_LN832404.1.
PDB; 2LLZ; NMR; -; A=1-98.
PDBsum; 2LLZ; -.
ProteinModelPortal; P0AF61; -.
SMR; P0AF61; -.
BioGrid; 4261767; 11.
STRING; 316385.ECDH10B_4320; -.
PaxDb; P0AF61; -.
PRIDE; P0AF61; -.
EnsemblBacteria; AAC77089; AAC77089; b4128.
EnsemblBacteria; BAE78130; BAE78130; BAE78130.
GeneID; 948646; -.
KEGG; ecj:JW4089; -.
KEGG; eco:b4128; -.
PATRIC; fig|511145.12.peg.4259; -.
EchoBASE; EB2361; -.
EcoGene; EG12468; ghoS.
HOGENOM; HOG000127074; -.
KO; K18840; -.
BioCyc; EcoCyc:G7830-MONOMER; -.
BioCyc; MetaCyc:G7830-MONOMER; -.
PRO; PR:P0AF61; -.
Proteomes; UP000000318; Chromosome.
Proteomes; UP000000625; Chromosome.
GO; GO:0004521; F:endoribonuclease activity; IDA:EcoCyc.
GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-KW.
GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
InterPro; IPR022597; GhoS.
Pfam; PF11080; GhoS; 1.
1: Evidence at protein level;
3D-structure; Complete proteome; Endonuclease; Hydrolase; Nuclease;
Reference proteome; Transcription; Transcription regulation.
CHAIN 1 98 Endoribonuclease antitoxin GhoS.
/FTId=PRO_0000169733.
MUTAGEN 14 14 F->A: Still digests ghoT RNA.
{ECO:0000269|PubMed:22941047}.
MUTAGEN 15 15 D->A: Still digests ghoT RNA.
{ECO:0000269|PubMed:22941047}.
MUTAGEN 25 25 L->I: Protein becomes toxic upon
overexpression. Becomes classic type II
TA toxin protein, increased cell
persistence to ampicillin; when
associated with L-31.
{ECO:0000269|PubMed:24797297}.
MUTAGEN 26 26 R->A: Slightly impaired digestion of ghoT
RNA. {ECO:0000269|PubMed:22941047}.
MUTAGEN 28 28 R->A: Reduced digestion of ghoT RNA, less
efficient neutralization of GhoT in vivo.
{ECO:0000269|PubMed:22941047}.
MUTAGEN 31 31 M->L: Protein becomes toxic upon
overexpression. Becomes classic type II
TA toxin protein, increased cell
persistence to ampicillin; when
associated with I-25.
{ECO:0000269|PubMed:24797297}.
MUTAGEN 55 55 F->A: Reduced digestion of ghoT RNA.
{ECO:0000269|PubMed:22941047}.
STRAND 10 15 {ECO:0000244|PDB:2LLZ}.
HELIX 18 20 {ECO:0000244|PDB:2LLZ}.
HELIX 21 33 {ECO:0000244|PDB:2LLZ}.
STRAND 36 41 {ECO:0000244|PDB:2LLZ}.
STRAND 43 45 {ECO:0000244|PDB:2LLZ}.
STRAND 47 49 {ECO:0000244|PDB:2LLZ}.
STRAND 53 57 {ECO:0000244|PDB:2LLZ}.
HELIX 63 72 {ECO:0000244|PDB:2LLZ}.
HELIX 73 75 {ECO:0000244|PDB:2LLZ}.
STRAND 83 88 {ECO:0000244|PDB:2LLZ}.
HELIX 89 93 {ECO:0000244|PDB:2LLZ}.
SEQUENCE 98 AA; 11468 MW; 8466741C8225E468 CRC64;
MEGKNKFNTY VVSFDYPSSY SSVFLRLRSL MYDMNFSSIV ADEYGIPRQL NENSFAITTS
LAASEIEDLI RLKCLDLPDI DFDLNIMTVD DYFRQFYK


Related products :

Catalog number Product name Quantity
GWB-73E000 Corynebacterium diphtheriae toxin 5S IgG (antitoxin) quantitative
ELI-5689 Diphteria (antitoxin) (Corynebacterium Diphteriae toxin) IgG 96 Wells
ELI-5331 Diphteria (antitoxin) (Corynebacterium Diphteriae toxin) IgG 96 Wells
GWB-7F39D2 Corynebacterium diptheriae toxin 5S IgG plus (antitoxin) quantitative
GWB-F06CAF Corynebacterium diphtheriae toxin IgG (antitoxin) quantitative
ELI-5689 Diphteria (antitoxin) (Corynebacterium Diphteriae toxin) IgG, Virology-Serology 10 kits
ELI-5331 Diphteria (antitoxin) (Corynebacterium Diphteriae toxin) IgG, Virology-Serology 10 kits
TTOX18-A Anti-C. tetani purified toxin Toxoid IgG (Tetanus antitoxin, neutralizing, 300 IU ml) 100 ul
TTOX19-A Anti-C. tetani purified toxin Toxoid IgG (Tetanus antitoxin, neutralizing, 750 IU ml) 100 ul
ELI-5379 Tetanus toxin (antitoxin) IgG ELISA with CE; incub. time 60_30_15 min; Sample Serum 96 Wells
TTOX20-Fab2 Anti-C. tetani purified toxin Toxoid IgG (Fab2), Tetanus antitoxin (neutralizing) 100 ul
EIAAB12950 Bos taurus,Bovine,ENDOU,Poly(U)-specific endoribonuclease,Protein endoU,Uridylate-specific endoribonuclease
EIAAB12952 ENDOU,Homo sapiens,Human,Placental protein 11,Poly(U)-specific endoribonuclease,PP11,Protein endoU,Uridylate-specific endoribonuclease
AP11794PU-N Endoribonuclease dcr_1 (Center) 0.1 mg
EH731 Endoribonuclease Dicer Elisa Kit 96T
AP11793PU-N Endoribonuclease dcr_1 (C_term) 0.1 mg
AP11794PU-N Endoribonuclease dcr_1 (Center) 0.1 mg
AP11793PU-N Endoribonuclease dcr_1 (C_term) 0.1 mg
'AP11794CP-N Endoribonuclease dcr-1 Control Peptide antibody _CP 0.1 mg
AP11793CP-N Endoribonuclease dcr_1 Control Peptide 0.1 mg
'AP11793CP-N Endoribonuclease dcr-1 Control Peptide antibody Ab CP 0.1 mg
AP11794CP-N Endoribonuclease dcr_1 Control Peptide 0.1 mg
'AP11794CP-N Endoribonuclease dcr-1 Control Peptide antibody Ab CP 0.1 mg
'AP11794CP-N Endoribonuclease dcr-1 Control Peptide antibody CP 0.1 mg
'AP11793CP-N Endoribonuclease dcr-1 Control Peptide antibody CP 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur