Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Evolved beta-galactosidase subunit alpha (Beta-gal) (EC 3.2.1.23) (Lactase)

 BGA2_ECOLI              Reviewed;        1030 AA.
P06864; P76660; Q2M9D0; Q6BF50;
01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
11-OCT-2004, sequence version 4.
28-MAR-2018, entry version 161.
RecName: Full=Evolved beta-galactosidase subunit alpha;
Short=Beta-gal;
EC=3.2.1.23;
AltName: Full=Lactase;
Name=ebgA; OrderedLocusNames=b3076, JW5511;
Escherichia coli (strain K12).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=83333;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2515108;
Hall B.G., Betts P.W., Wootton J.C.;
"DNA sequence analysis of artificially evolved ebg enzyme and ebg
repressor genes.";
Genetics 123:635-648(1989).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3939707;
Stokes H.W., Betts P.W., Hall B.G.;
"Sequence of the ebgA gene of Escherichia coli: comparison with the
lacZ gene.";
Mol. Biol. Evol. 2:469-477(1985).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=9278503; DOI=10.1126/science.277.5331.1453;
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1462(1997).
[4]
SEQUENCE REVISION TO 275 AND 891-892.
PubMed=16397293; DOI=10.1093/nar/gkj405;
Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H.,
Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.;
"Escherichia coli K-12: a cooperatively developed annotation snapshot
-- 2005.";
Nucleic Acids Res. 34:1-9(2006).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=16738553; DOI=10.1038/msb4100049;
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains
MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[6]
ACTIVE SITE REGIONS.
PubMed=6411710;
Fowler A.V., Smith P.J.;
"The active site regions of lacZ and ebg beta-galactosidases are
homologous.";
J. Biol. Chem. 258:10204-10207(1983).
[7]
SUBUNIT.
PubMed=1540130; DOI=10.1042/bj2820155;
Elliott A.C., Sinnott M.L., Smith P.J., Bommuswamy J., Guo Z.,
Hall B.G., Zhang Y.;
"The catalytic consequences of experimental evolution. Studies on the
subunit structure of the second (ebg) beta-galactosidase of
Escherichia coli, and on catalysis by ebgab, an experimental evolvant
containing two amino acid substitutions.";
Biochem. J. 282:155-164(1992).
-!- FUNCTION: The wild-type enzyme is an ineffective lactase. Two
classes of point mutations dramatically improve activity of the
enzyme.
-!- CATALYTIC ACTIVITY: Hydrolysis of terminal non-reducing beta-D-
galactose residues in beta-D-galactosides.
-!- SUBUNIT: Heterooctamer of 4 alpha and 4 beta subunits.
{ECO:0000269|PubMed:1540130}.
-!- INTERACTION:
P32053:intA; NbExp=3; IntAct=EBI-558098, EBI-552967;
-!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
{ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=AAA57877.1; Type=Erroneous initiation; Evidence={ECO:0000305};
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; M64441; AAA61971.1; -; Genomic_DNA.
EMBL; X52031; CAA36274.1; -; Genomic_DNA.
EMBL; X03228; CAA26977.1; ALT_SEQ; Genomic_DNA.
EMBL; U18997; AAA57877.1; ALT_INIT; Genomic_DNA.
EMBL; U00096; AAT48164.1; -; Genomic_DNA.
EMBL; AP009048; BAE77126.1; -; Genomic_DNA.
PIR; A65096; GBECE.
RefSeq; WP_001082856.1; NZ_LN832404.1.
RefSeq; YP_026199.1; NC_000913.3.
ProteinModelPortal; P06864; -.
BioGrid; 4263419; 6.
DIP; DIP-2893N; -.
IntAct; P06864; 7.
STRING; 316385.ECDH10B_3251; -.
DrugBank; DB00581; Lactulose.
CAZy; GH2; Glycoside Hydrolase Family 2.
PaxDb; P06864; -.
PRIDE; P06864; -.
EnsemblBacteria; AAT48164; AAT48164; b3076.
EnsemblBacteria; BAE77126; BAE77126; BAE77126.
GeneID; 947583; -.
KEGG; ecj:JW5511; -.
KEGG; eco:b3076; -.
PATRIC; fig|511145.12.peg.3170; -.
EchoBASE; EB0248; -.
EcoGene; EG10252; ebgA.
eggNOG; ENOG4105CNT; Bacteria.
eggNOG; COG3250; LUCA.
HOGENOM; HOG000252444; -.
InParanoid; P06864; -.
KO; K12111; -.
OMA; WCDHGIL; -.
PhylomeDB; P06864; -.
BioCyc; EcoCyc:EG10252-MONOMER; -.
SABIO-RK; P06864; -.
PRO; PR:P06864; -.
Proteomes; UP000000318; Chromosome.
Proteomes; UP000000625; Chromosome.
GO; GO:0009341; C:beta-galactosidase complex; IBA:GO_Central.
GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
GO; GO:0005990; P:lactose catabolic process; IBA:GO_Central.
Gene3D; 2.60.120.260; -; 1.
Gene3D; 2.60.40.10; -; 2.
Gene3D; 2.70.98.10; -; 1.
InterPro; IPR004199; B-gal_small/dom_5.
InterPro; IPR036156; Beta-gal/glucu_dom_sf.
InterPro; IPR011013; Gal_mutarotase_sf_dom.
InterPro; IPR008979; Galactose-bd-like_sf.
InterPro; IPR014718; GH-type_carb-bd.
InterPro; IPR006101; Glyco_hydro_2.
InterPro; IPR023232; Glyco_hydro_2_AS.
InterPro; IPR006103; Glyco_hydro_2_cat.
InterPro; IPR023230; Glyco_hydro_2_CS.
InterPro; IPR006102; Glyco_hydro_2_Ig-like.
InterPro; IPR006104; Glyco_hydro_2_N.
InterPro; IPR017853; Glycoside_hydrolase_SF.
InterPro; IPR013783; Ig-like_fold.
InterPro; IPR032312; LacZ_4.
Pfam; PF02929; Bgal_small_N; 1.
Pfam; PF16353; DUF4981; 1.
Pfam; PF00703; Glyco_hydro_2; 1.
Pfam; PF02836; Glyco_hydro_2_C; 1.
Pfam; PF02837; Glyco_hydro_2_N; 1.
PRINTS; PR00132; GLHYDRLASE2.
SMART; SM01038; Bgal_small_N; 1.
SUPFAM; SSF49303; SSF49303; 2.
SUPFAM; SSF49785; SSF49785; 1.
SUPFAM; SSF51445; SSF51445; 1.
SUPFAM; SSF74650; SSF74650; 1.
PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
1: Evidence at protein level;
Complete proteome; Glycosidase; Hydrolase; Reference proteome.
CHAIN 1 1030 Evolved beta-galactosidase subunit alpha.
/FTId=PRO_0000057651.
ACT_SITE 449 449 Proton donor.
{ECO:0000269|PubMed:6411710}.
ACT_SITE 512 512 Nucleophile. {ECO:0000250}.
VARIANT 92 92 D -> N (improve activity).
VARIANT 93 93 E -> K (improve activity).
VARIANT 976 976 W -> C (improve activity).
VARIANT 978 978 S -> G (improve activity).
CONFLICT 275 275 S -> T (in Ref. 1, 2 and 3; AAA57877).
{ECO:0000305}.
CONFLICT 465 465 A -> R (in Ref. 1 and 2). {ECO:0000305}.
CONFLICT 640 640 T -> S (in Ref. 1 and 2). {ECO:0000305}.
CONFLICT 649 649 R -> P (in Ref. 1 and 2). {ECO:0000305}.
CONFLICT 767 767 M -> MM (in Ref. 1 and 2). {ECO:0000305}.
CONFLICT 891 892 ST -> QA (in Ref. 1, 2 and 3; AAA57877).
{ECO:0000305}.
CONFLICT 1026 1026 S -> T (in Ref. 1 and 2). {ECO:0000305}.
SEQUENCE 1030 AA; 117879 MW; FEC4D7A558C6EE94 CRC64;
MNRWENIQLT HENRLAPRAY FFSYDSVAQA RTFARETSSL FLPLSGQWNF HFFDHPLQVP
EAFTSELMAD WGHITVPAMW QMEGHGKLQY TDEGFPFPID VPFVPSDNPT GAYQRIFTLS
DGWQGKQTLI KFDGVETYFE VYVNGQYVGF SKGSRLTAEF DISAMVKTGD NLLCVRVMQW
ADSTYVEDQD MWWSAGIFRD VYLVGKHLTH INDFTVRTDF DEAYCDATLS CEVVLENLAA
SPVVTTLEYT LFDGERVVHS SAIDHLAIEK LTSASFAFTV EQPQQWSAES PYLYHLVMTL
KDANGNVLEV VPQRVGFRDI KVRDGLFWIN NRYVMLHGVN RHDNDHRKGR AVGMDRVEKD
LQLMKQHNIN SVRTAHYPND PRFYELCDIY GLFVMAETDV ESHGFANVGD ISRITDDPQW
EKVYVERIVR HIHAQKNHPS IIIWSLGNES GYGCNIRAMY HAAKALDDTR LVHYEEDRDA
EVVDIISTMY TRVPLMNEFG EYPHPKPRII CEYAHAMGNG PGGLTEYQNV FYKHDCIQGH
YVWEWCDHGI QAQDDHGNVW YKFGGDYGDY PNNYNFCLDG LIYSDQTPGP GLKEYKQVIA
PVKIHARDLT RGELKVENKL WFTTLDDYTL HAEVRAEGET LATQQIKLRD VAPNSEAPLQ
ITLPQLDARE AFLNITVTKD SRTRYSEAGH PIATYQFPLK ENTAQPVPFA PNNARPLTLE
DDRLSCTVRG YNFAITFSKM SGKPTSWQVN GESLLTREPK INFFKPMIDN HKQEYEGLWQ
PNHLQIMQEH LRDFAVEQSD GEVLIISRTV IAPPVFDFGM RCTYIWRIAA DGQVNVALSG
ERYGDYPHII PCIGFTMGIN GEYDQVAYYG RGPGENYADS QQANIIDIWR STVDAMFENY
PFPQNNGNRQ HVRWTALTNR HGNGLLVVPQ RPINFSAWHY TQENIHAAQH CNELQRSDDI
TLNLDHQLLG LGSNSWGSEV LDSWRVWFRD FSYGFTLLPV SGGEATAQSL ASYEFGAGFF
STNLHSENKQ


Related products :

Catalog number Product name Quantity
E0196h ELISA Acid beta-galactosidase,Beta-galactosidase,Elastin receptor 1,ELNR1,GLB1,Homo sapiens,Human,Lactase 96T
E0196h ELISA kit Acid beta-galactosidase,Beta-galactosidase,Elastin receptor 1,ELNR1,GLB1,Homo sapiens,Human,Lactase 96T
U0196h CLIA Acid beta-galactosidase,Beta-galactosidase,Elastin receptor 1,ELNR1,GLB1,Homo sapiens,Human,Lactase 96T
E0196m ELISA Acid beta-galactosidase,Beta-galactosidase,Bgl,Glb1,Glb-1,Lactase,Mouse,Mus musculus 96T
E0196b ELISA Acid beta-galactosidase,Beta-galactosidase,Bos taurus,Bovine,GLB1,Lactase 96T
E0196b ELISA kit Acid beta-galactosidase,Beta-galactosidase,Bos taurus,Bovine,GLB1,Lactase 96T
U0196m CLIA Acid beta-galactosidase,Beta-galactosidase,Bgl,Glb1,Glb-1,Lactase,Mouse,Mus musculus 96T
E0196m ELISA kit Acid beta-galactosidase,Beta-galactosidase,Bgl,Glb1,Glb-1,Lactase,Mouse,Mus musculus 96T
U0196b CLIA Acid beta-galactosidase,Beta-galactosidase,Bos taurus,Bovine,GLB1,Lactase 96T
U0196c CLIA Acid beta-galactosidase,Beta-galactosidase,Canis familiaris,Canis lupus familiaris,Dog,GLB1,Lactase 96T
E0196c ELISA kit Acid beta-galactosidase,Beta-galactosidase,Canis familiaris,Canis lupus familiaris,Dog,GLB1,Lactase 96T
E0196c ELISA Acid beta-galactosidase,Beta-galactosidase,Canis familiaris,Canis lupus familiaris,Dog,GLB1,Lactase 96T
18-783-78938 RABBIT ANTI BETA GALACTOSIDASE HRP - EC 3.2.1.23; Lactase Polyclonal 1 mg
18-783-75642 RABBIT ANTI BETA GALACTOSIDASE - EC 3.2.1.23; Lactase Polyclonal 1 mg
18-783-78935 RABBIT ANTI BETA GALACTOSIDASE FITC - EC 3.2.1.23; Lactase Polyclonal 1 mg
18-783-78911 RABBIT ANTI BETA GALACTOSIDASE Biotin - EC 3.2.1.23; Lactase Polyclonal 1 mg
20-783-73296 MOUSE ANTI ESCHERICHIA COLI BETA GALACTOSIDASE - EC 3.2.1.23; Lactase Monoclonal 0.2 mg
E0195h ELISA Beta-hexosaminidase subunit alpha,Beta-N-acetylhexosaminidase subunit alpha,HEXA,Hexosaminidase subunit A,Homo sapiens,Human,N-acetyl-beta-glucosaminidase subunit alpha 96T
U0195h CLIA Beta-hexosaminidase subunit alpha,Beta-N-acetylhexosaminidase subunit alpha,HEXA,Hexosaminidase subunit A,Homo sapiens,Human,N-acetyl-beta-glucosaminidase subunit alpha 96T
E0195h ELISA kit Beta-hexosaminidase subunit alpha,Beta-N-acetylhexosaminidase subunit alpha,HEXA,Hexosaminidase subunit A,Homo sapiens,Human,N-acetyl-beta-glucosaminidase subunit alpha 96T
U0195m CLIA Beta-hexosaminidase subunit alpha,Beta-N-acetylhexosaminidase subunit alpha,Hexa,Hexosaminidase subunit A,Mouse,Mus musculus,N-acetyl-beta-glucosaminidase subunit alpha 96T
U0195b CLIA Beta-hexosaminidase subunit alpha,Beta-N-acetylhexosaminidase subunit alpha,Bos taurus,Bovine,HEXA,Hexosaminidase subunit A,N-acetyl-beta-glucosaminidase subunit alpha 96T
U0195r CLIA Beta-hexosaminidase subunit alpha,Beta-N-acetylhexosaminidase subunit alpha,Hexa,Hexosaminidase subunit A,N-acetyl-beta-glucosaminidase subunit alpha,Rat,Rattus norvegicus 96T
E0195m ELISA kit Beta-hexosaminidase subunit alpha,Beta-N-acetylhexosaminidase subunit alpha,Hexa,Hexosaminidase subunit A,Mouse,Mus musculus,N-acetyl-beta-glucosaminidase subunit alpha 96T
E0195r ELISA kit Beta-hexosaminidase subunit alpha,Beta-N-acetylhexosaminidase subunit alpha,Hexa,Hexosaminidase subunit A,N-acetyl-beta-glucosaminidase subunit alpha,Rat,Rattus norvegicus 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur