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Folate synthesis bifunctional protein [Includes: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) (EC 2.7.6.3) (2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase) (7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase) (PPPK); Dihydropteroate synthase (DHPS) (EC 2.5.1.15)]

 FOLC_ARATH              Reviewed;         484 AA.
Q1ENB6; Q9LE77;
29-APR-2015, integrated into UniProtKB/Swiss-Prot.
11-JUL-2006, sequence version 1.
25-OCT-2017, entry version 95.
RecName: Full=Folate synthesis bifunctional protein {ECO:0000303|PubMed:17289662};
Includes:
RecName: Full=6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase {ECO:0000303|PubMed:17289662};
Short=HPPK {ECO:0000303|PubMed:17289662};
EC=2.7.6.3 {ECO:0000269|PubMed:17289662};
AltName: Full=2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase;
AltName: Full=7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase;
Short=PPPK;
Includes:
RecName: Full=Dihydropteroate synthase {ECO:0000303|PubMed:17289662};
Short=DHPS {ECO:0000303|PubMed:17289662};
EC=2.5.1.15 {ECO:0000269|PubMed:17289662};
Name=CytHPPK/DHPS {ECO:0000303|PubMed:17289662};
OrderedLocusNames=At1g69190 {ECO:0000312|Araport:AT1G69190};
ORFNames=F23O10.22 {ECO:0000312|EMBL:AAG52504.1},
F4N2.15 {ECO:0000312|EMBL:AAF27067.1};
Arabidopsis thaliana (Mouse-ear cress).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
Arabidopsis.
NCBI_TaxID=3702 {ECO:0000312|EMBL:CAI29255.1};
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY,
BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR
LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION BY SALT
STRESS, AND DISRUPTION PHENOTYPE.
STRAIN=cv. Columbia;
PubMed=17289662; DOI=10.1074/jbc.M701158200;
Storozhenko S., Navarrete O., Ravanel S., De Brouwer V., Chaerle P.,
Zhang G.F., Bastien O., Lambert W., Rebeille F., Van Der Straeten D.;
"Cytosolic hydroxymethyldihydropterin
pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana:
a specific role in early development and stress response.";
J. Biol. Chem. 282:10749-10761(2007).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
PubMed=11130712; DOI=10.1038/35048500;
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis
thaliana.";
Nature 408:816-820(2000).
[3]
GENOME REANNOTATION.
STRAIN=cv. Columbia;
The Arabidopsis Information Portal (Araport);
Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
[4]
FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY OXIDATIVE STRESS.
PubMed=21996493; DOI=10.1016/j.phytochem.2011.09.008;
Navarrete O., Van Daele J., Stove C., Lambert W., Van Der Straeten D.,
Storozhenko S.;
"A folate independent role for cytosolic HPPK/DHPS upon stress in
Arabidopsis thaliana.";
Phytochemistry 73:23-33(2012).
-!- FUNCTION: Catalyzes the first two consecutive steps of
tetrahydrofolate biosynthesis (PubMed:17289662). Plays a role in
seed stress response and survival (PubMed:17289662,
PubMed:21996493). {ECO:0000269|PubMed:17289662,
ECO:0000269|PubMed:21996493}.
-!- CATALYTIC ACTIVITY: ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP
+ 6-hydroxymethyl-7,8-dihydropterin diphosphate.
{ECO:0000269|PubMed:17289662}.
-!- CATALYTIC ACTIVITY: 6-hydroxymethyl-7,8-dihydropterin diphosphate
+ 4-aminobenzoate = diphosphate + dihydropteroate.
{ECO:0000269|PubMed:17289662}.
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Evidence={ECO:0000250|UniProtKB:P0AC13};
-!- ENZYME REGULATION: Inhibited by sulfanilamide.
{ECO:0000269|PubMed:17289662}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=30 uM for ATP {ECO:0000269|PubMed:17289662};
-!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-
amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate
from 7,8-dihydroneopterin triphosphate: step 4/4. {ECO:0000305}.
-!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-
dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.
{ECO:0000305}.
-!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
{ECO:0000269|PubMed:17289662}.
-!- TISSUE SPECIFICITY: Expressed exclusively in reproductive tissues.
{ECO:0000269|PubMed:17289662}.
-!- DEVELOPMENTAL STAGE: Expressed in early stages of seed
development, until the late globular stage of embryo. Not detected
at the heart stage of embryo development.
{ECO:0000269|PubMed:17289662}.
-!- INDUCTION: Up-regulated by slat stress (PubMed:17289662). Up-
regulated by oxidative stress (PubMed:21996493).
{ECO:0000269|PubMed:17289662, ECO:0000269|PubMed:21996493}.
-!- DISRUPTION PHENOTYPE: No visible phenotype (PubMed:17289662).
Increased sensitivity to oxidative stress (PubMed:21996493).
{ECO:0000269|PubMed:17289662, ECO:0000269|PubMed:21996493}.
-!- SIMILARITY: In the N-terminal section; belongs to the HPPK family.
{ECO:0000305}.
-!- SIMILARITY: In the C-terminal section; belongs to the DHPS family.
{ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=AAF27067.1; Type=Erroneous gene model prediction; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Sequence=AAG52504.1; Type=Erroneous gene model prediction; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
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EMBL; AJ866732; CAI29255.1; -; mRNA.
EMBL; AC008262; AAF27067.1; ALT_SEQ; Genomic_DNA.
EMBL; AC018364; AAG52504.1; ALT_SEQ; Genomic_DNA.
EMBL; CP002684; AEE34892.1; -; Genomic_DNA.
PIR; H96715; H96715.
RefSeq; NP_001319354.1; NM_001334402.1.
UniGene; At.70808; -.
ProteinModelPortal; Q1ENB6; -.
SMR; Q1ENB6; -.
STRING; 3702.AT1G69190.1; -.
iPTMnet; Q1ENB6; -.
PaxDb; Q1ENB6; -.
EnsemblPlants; AT1G69190.1; AT1G69190.1; AT1G69190.
GeneID; 843250; -.
Gramene; AT1G69190.1; AT1G69190.1; AT1G69190.
KEGG; ath:AT1G69190; -.
Araport; AT1G69190; -.
TAIR; locus:2026471; AT1G69190.
eggNOG; KOG2544; Eukaryota.
eggNOG; COG0294; LUCA.
eggNOG; COG0801; LUCA.
HOGENOM; HOG000217511; -.
KO; K13941; -.
OMA; DHIACSH; -.
OrthoDB; EOG093607KB; -.
PhylomeDB; Q1ENB6; -.
BioCyc; ARA:AT1G69190-MONOMER; -.
BRENDA; 2.5.1.15; 302.
UniPathway; UPA00077; UER00155.
UniPathway; UPA00077; UER00156.
PRO; PR:Q1ENB6; -.
Proteomes; UP000006548; Chromosome 1.
Genevisible; Q1ENB6; AT.
GO; GO:0005829; C:cytosol; IDA:TAIR.
GO; GO:0003848; F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity; IDA:TAIR.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0004156; F:dihydropteroate synthase activity; IDA:TAIR.
GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0046656; P:folic acid biosynthetic process; IEA:UniProtKB-KW.
GO; GO:0006979; P:response to oxidative stress; IMP:TAIR.
GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IDA:TAIR.
CDD; cd00739; DHPS; 1.
CDD; cd00483; HPPK; 1.
Gene3D; 3.20.20.20; -; 1.
Gene3D; 3.30.70.560; -; 1.
InterPro; IPR006390; DHP_synth.
InterPro; IPR011005; Dihydropteroate_synth-like.
InterPro; IPR000550; Hppk.
InterPro; IPR035907; Hppk_sf.
InterPro; IPR000489; Pterin-binding_dom.
Pfam; PF01288; HPPK; 1.
Pfam; PF00809; Pterin_bind; 1.
SUPFAM; SSF51717; SSF51717; 1.
SUPFAM; SSF55083; SSF55083; 1.
TIGRFAMs; TIGR01496; DHPS; 1.
TIGRFAMs; TIGR01498; folK; 1.
PROSITE; PS00792; DHPS_1; 1.
PROSITE; PS00793; DHPS_2; 1.
PROSITE; PS00794; HPPK; 1.
PROSITE; PS50972; PTERIN_BINDING; 1.
1: Evidence at protein level;
ATP-binding; Complete proteome; Cytoplasm; Folate biosynthesis;
Kinase; Magnesium; Metal-binding; Multifunctional enzyme;
Nucleotide-binding; Reference proteome; Transferase.
CHAIN 1 484 Folate synthesis bifunctional protein.
/FTId=PRO_0000432867.
DOMAIN 202 470 Pterin-binding. {ECO:0000255|PROSITE-
ProRule:PRU00334}.
REGION 15 141 HPPK. {ECO:0000305}.
REGION 204 484 DHPS.
REGION 458 460 6-hydroxymethyl-7,8-dihydropterin
diphosphate binding.
{ECO:0000250|UniProtKB:P0AC13}.
METAL 209 209 Magnesium.
{ECO:0000250|UniProtKB:P9WND1}.
BINDING 249 249 6-hydroxymethyl-7,8-dihydropterin
diphosphate.
{ECO:0000250|UniProtKB:P0AC13}.
BINDING 286 286 6-hydroxymethyl-7,8-dihydropterin
diphosphate.
{ECO:0000250|UniProtKB:P0AC13}.
BINDING 305 305 6-hydroxymethyl-7,8-dihydropterin
diphosphate.
{ECO:0000250|UniProtKB:P0AC13}.
BINDING 378 378 6-hydroxymethyl-7,8-dihydropterin
diphosphate.
{ECO:0000250|UniProtKB:P0AC13}.
BINDING 423 423 6-hydroxymethyl-7,8-dihydropterin
diphosphate.
{ECO:0000250|UniProtKB:P0AC13}.
SEQUENCE 484 AA; 54110 MW; 3F9985DBA8655369 CRC64;
MDFTSLETTT FEEVVIALGS NVGNRMNNFK EALRLMKDYG ISVTRHSCLY ETEPVHVTDQ
PRFLNAAIRG VTKLKPHELL NVLKKIEKEM GREENGLRYG PRPLDLDILF YGKHKIISDK
LIIPHERIWE RPFVLAPLVD LLGTEDIDND KIVAYWHSLS MHSGGIFQAW ERLGGESLLG
KDGIIQRVIP IGDHLWDFSK KTYVMGILNL TPDSFSDGGK FQSVDTAVSR VRSMISEGVD
IIDIGAQSTR PMASRISSQE EIDRLIPVLK VVRGMAEMKG KLISVDTFNS EVALEAIRNG
ADILNDVSGG SLDENMHKVV ADSDVPYMIM HMRGDPCTMQ NKENLEYNEI CKDVATELYE
RVREAELSGI PAWRIMIDPG IGFSKGIDHN LDIVMELPKI REEMAKKSIG LSHAPILIGP
SRKRFLGDIC GRPEASERDA ATVACVTAGI LKGANIIRVH NVRDNVDAAR LCDAMMTKRF
KNVD


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