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GFP-like non-fluorescent chromoprotein (cgCP)

 NFCP_CONGI              Reviewed;         227 AA.
Q95W86;
23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
01-DEC-2001, sequence version 1.
22-NOV-2017, entry version 51.
RecName: Full=GFP-like non-fluorescent chromoprotein;
AltName: Full=cgCP;
Condylactis gigantea (Giant Caribbean anemone).
Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
Nynantheae; Actiniidae; Condylactis.
NCBI_TaxID=47073;
[1] {ECO:0000305, ECO:0000312|EMBL:AAL27537.1}
NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, AND MUTAGENESIS OF CYS-143.
PubMed=11682051; DOI=10.1016/S0014-5793(01)02930-1;
Gurskaya N.G., Fradkov A.F., Terskikh A., Matz M.V., Labas Y.A.,
Martynov V.I., Yanushevich Y.G., Lukyanov K.A., Lukyanov S.A.;
"GFP-like chromoproteins as a source of far-red fluorescent
proteins.";
FEBS Lett. 507:16-20(2001).
-!- FUNCTION: Non-fluorescent pigment protein that is mauve in color.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Absorption:
Abs(max)=571 nm;
-!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:11682051}.
-!- PTM: Contains a chromophore consisting of modified amino acid
residues. The chromophore is formed by autocatalytic backbone
condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-
(N+1) to didehydrotyrosine. Maturation of the chromophore requires
nothing other than molecular oxygen. The precise stereochemistry
of the tyrosine has not been determined.
-!- BIOTECHNOLOGY: Fluorescent proteins have become a useful and
ubiquitous tool for making chimeric proteins, where they function
as a fluorescent protein tag. Typically they tolerate N- and C-
terminal fusion to a broad variety of proteins. They have been
expressed in most known cell types and are used as a noninvasive
fluorescent marker in living cells and organisms. They enable a
wide range of applications where they have functioned as a cell
lineage tracer, reporter of gene expression, or as a measure of
protein-protein interactions. {ECO:0000305}.
-!- MISCELLANEOUS: Fluorescence excitation of the Ser-143 mutant is at
587 nm and emission at 622 nm.
-!- SIMILARITY: Belongs to the GFP family. {ECO:0000305}.
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EMBL; AF363775; AAL27537.1; -; mRNA.
ProteinModelPortal; Q95W86; -.
SMR; Q95W86; -.
GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
GO; GO:0018298; P:protein-chromophore linkage; IEA:UniProtKB-KW.
InterPro; IPR009017; GFP.
InterPro; IPR011584; GFP-related.
Pfam; PF01353; GFP; 1.
SUPFAM; SSF54511; SSF54511; 1.
1: Evidence at protein level;
Chromophore; Luminescence; Photoprotein.
CHAIN 1 227 GFP-like non-fluorescent chromoprotein.
/FTId=PRO_0000192583.
MOD_RES 64 64 2,3-didehydrotyrosine.
{ECO:0000250|UniProtKB:P83690}.
CROSSLNK 63 65 5-imidazolinone (Ala-Gly). {ECO:0000250}.
MUTAGEN 143 143 C->S: Produces a fluorescent form.
{ECO:0000269|PubMed:11682051}.
SEQUENCE 227 AA; 25416 MW; BCFA5A4CBCE1B3F7 CRC64;
MAGLLKESMR IKIYMEGTVN GYHFKCEGEG DGNPFEGTQN MRIRVTEGAP LPFAFDILSP
CCAYGSKTFI KHTSGIPDYF KQSFPEGFTW ERTTIYEDGG VLTAHQDTSL EGNCLIYKVK
VLGTNFPADG PVMKKISGGW EPCTEIVYQD NGVLRGRNVM ALKVSGRPPL ICHLHSTYRS
KKACALTMPG FHFADLRIQM PKKKKDEYFE LYEASVARYS DVPEKAT


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