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General negative regulator of transcription subunit 2 (cell division cycle protein 36)

 NOT2_YEAST              Reviewed;         191 AA.
P06100; D6VRI6;
01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
01-JAN-1988, sequence version 1.
25-OCT-2017, entry version 149.
RecName: Full=General negative regulator of transcription subunit 2;
AltName: Full=cell division cycle protein 36;
Name=CDC36; Synonyms=DNA19, NOT2; OrderedLocusNames=YDL165W;
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
NCBI_TaxID=559292;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3018676; DOI=10.1093/nar/14.16.6681;
Ferguson J., Ho J.-Y., Peterson T.A., Reed S.I.;
"Nucleotide sequence of the yeast cell division cycle start genes
CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the
predicted products.";
Nucleic Acids Res. 14:6681-6697(1986).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3909103; DOI=10.1093/nar/13.23.8323;
Barker D.G., White J.H.M., Johnston L.H.;
"The nucleotide sequence of the DNA ligase gene (CDC9) from
Saccharomyces cerevisiae: a gene which is cell-cycle regulated and
induced in response to DNA damage.";
Nucleic Acids Res. 13:8323-8337(1985).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169867;
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N.,
Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M.,
Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L.,
Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M.,
Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S.,
Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M.,
Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S.,
Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K.,
Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D.,
Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C.,
Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T.,
Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E.,
Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W.,
Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K.,
Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S.,
Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A.,
Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S.,
Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M.,
Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y.,
Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M.,
Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E.,
Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R.,
Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
Mewes H.-W., Zollner A., Zaccaria P.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
Nature 387:75-78(1997).
[4]
GENOME REANNOTATION.
STRAIN=ATCC 204508 / S288c;
PubMed=24374639; DOI=10.1534/g3.113.008995;
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
Cherry J.M.;
"The reference genome sequence of Saccharomyces cerevisiae: Then and
now.";
G3 (Bethesda) 4:389-398(2014).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=17322287; DOI=10.1101/gr.6037607;
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-
encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[6]
CHARACTERIZATION.
PubMed=7926748; DOI=10.1101/gad.8.5.525;
Collart M.A., Struhl K.;
"NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative
regulator of transcription that differentially affects TATA-element
utilization.";
Genes Dev. 8:525-537(1994).
[7]
IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, AND FUNCTION OF THE
CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
PubMed=9463387; DOI=10.1093/emboj/17.4.1096;
Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M.,
Denis C.L.;
"The NOT proteins are part of the CCR4 transcriptional complex and
affect gene expression both positively and negatively.";
EMBO J. 17:1096-1106(1998).
[8]
INTERACTION WITH NOT1.
PubMed=10490603; DOI=10.1128/MCB.19.10.6642;
Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L.;
"The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and
functionally separated from NOT2, NOT4, and NOT5.";
Mol. Cell. Biol. 19:6642-6651(1999).
[9]
IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
PubMed=11733989; DOI=10.1006/jmbi.2001.5162;
Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.;
"Purification and characterization of the 1.0 MDa CCR4-NOT complex
identifies two novel components of the complex.";
J. Mol. Biol. 314:683-694(2001).
[10]
SUBCELLULAR LOCATION.
PubMed=11889048; DOI=10.1093/emboj/21.6.1427;
Tucker M., Staples R.R., Valencia-Sanchez M.A., Muhlrad D., Parker R.;
"Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase
complex in Saccharomyces cerevisiae.";
EMBO J. 21:1427-1436(2002).
[11]
INTERACTION WITH NOT5.
PubMed=12215412; DOI=10.1016/S0022-2836(02)00707-6;
Russell P., Benson J.D., Denis C.L.;
"Characterization of mutations in NOT2 indicates that it plays an
important role in maintaining the integrity of the CCR4-NOT complex.";
J. Mol. Biol. 322:27-39(2002).
[12]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
PubMed=14562106; DOI=10.1038/nature02046;
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
-!- FUNCTION: Acts as component of the CCR4-NOT core complex, which in
the nucleus seems to be a general transcription factor, and in the
cytoplasm the major mRNA deadenylase involved in mRNA turnover.
NOT2 is required for the integrity of the complex. The NOT protein
subcomplex negatively regulates the basal and activated
transcription of many genes. Preferentially affects TC-type TATA
element-dependent transcription. Could directly or indirectly
inhibit component(s) of the general transcription machinery.
{ECO:0000269|PubMed:9463387}.
-!- SUBUNIT: Forms a NOT protein complex that comprises NOT1, NOT2,
NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex
that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and
CAF130. In the complex interacts with NOT1 and NOT5. The core
complex probably is part of a less characterized 1.9 MDa CCR4-NOT
complex. {ECO:0000269|PubMed:10490603,
ECO:0000269|PubMed:11733989, ECO:0000269|PubMed:12215412,
ECO:0000269|PubMed:9463387}.
-!- INTERACTION:
P25655:CDC39; NbExp=7; IntAct=EBI-12153, EBI-12139;
P06102:NOT3; NbExp=2; IntAct=EBI-12153, EBI-12165;
Q12514:NOT5; NbExp=5; IntAct=EBI-12153, EBI-12184;
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11889048}.
Nucleus {ECO:0000269|PubMed:11889048}.
-!- MISCELLANEOUS: Present with 2120 molecules/cell in log phase SD
medium. {ECO:0000269|PubMed:14562106}.
-!- SIMILARITY: Belongs to the CNOT2/3/5 family. {ECO:0000305}.
-----------------------------------------------------------------------
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-----------------------------------------------------------------------
EMBL; X04287; CAA27835.1; -; Genomic_DNA.
EMBL; X03246; CAA27006.1; -; Genomic_DNA.
EMBL; Z67750; CAA91581.1; -; Genomic_DNA.
EMBL; Z74213; CAA98739.1; -; Genomic_DNA.
EMBL; X68020; CAA48160.1; -; Genomic_DNA.
EMBL; AY557644; AAS55970.1; -; Genomic_DNA.
EMBL; BK006938; DAA11696.1; -; Genomic_DNA.
PIR; S12304; RGBY36.
RefSeq; NP_010116.1; NM_001180225.1.
PDB; 4BY6; X-ray; 2.80 A; B/E=1-191.
PDBsum; 4BY6; -.
ProteinModelPortal; P06100; -.
SMR; P06100; -.
BioGrid; 31900; 228.
DIP; DIP-590N; -.
IntAct; P06100; 24.
MINT; MINT-400720; -.
STRING; 4932.YDL165W; -.
MaxQB; P06100; -.
PRIDE; P06100; -.
EnsemblFungi; YDL165W; YDL165W; YDL165W.
GeneID; 851389; -.
KEGG; sce:YDL165W; -.
EuPathDB; FungiDB:YDL165W; -.
SGD; S000002324; CDC36.
GeneTree; ENSGT00390000001285; -.
HOGENOM; HOG000246523; -.
InParanoid; P06100; -.
KO; K12605; -.
OMA; WAETSRS; -.
OrthoDB; EOG092C2LUV; -.
BioCyc; YEAST:G3O-29557-MONOMER; -.
PRO; PR:P06100; -.
Proteomes; UP000002311; Chromosome IV.
GO; GO:0030015; C:CCR4-NOT core complex; IPI:SGD.
GO; GO:0005737; C:cytoplasm; IDA:SGD.
GO; GO:0005634; C:nucleus; IDA:SGD.
GO; GO:0000932; C:P-body; IBA:GO_Central.
GO; GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IMP:SGD.
GO; GO:0031087; P:deadenylation-independent decapping of nuclear-transcribed mRNA; IMP:SGD.
GO; GO:0017148; P:negative regulation of translation; IBA:GO_Central.
GO; GO:0000288; P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IDA:SGD.
GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IDA:SGD.
GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD.
GO; GO:0016567; P:protein ubiquitination; IMP:SGD.
GO; GO:0051726; P:regulation of cell cycle; IMP:SGD.
GO; GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IPI:SGD.
GO; GO:0000749; P:response to pheromone involved in conjugation with cellular fusion; IMP:SGD.
GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IMP:SGD.
InterPro; IPR007282; NOT.
Pfam; PF04153; NOT2_3_5; 1.
1: Evidence at protein level;
3D-structure; Activator; Complete proteome; Cytoplasm; Nucleus;
Reference proteome; Repressor; Transcription;
Transcription regulation.
CHAIN 1 191 General negative regulator of
transcription subunit 2.
/FTId=PRO_0000198336.
STRAND 7 9 {ECO:0000244|PDB:4BY6}.
HELIX 35 40 {ECO:0000244|PDB:4BY6}.
HELIX 53 55 {ECO:0000244|PDB:4BY6}.
STRAND 59 61 {ECO:0000244|PDB:4BY6}.
HELIX 76 78 {ECO:0000244|PDB:4BY6}.
HELIX 84 86 {ECO:0000244|PDB:4BY6}.
STRAND 88 91 {ECO:0000244|PDB:4BY6}.
TURN 97 99 {ECO:0000244|PDB:4BY6}.
HELIX 101 104 {ECO:0000244|PDB:4BY6}.
HELIX 109 118 {ECO:0000244|PDB:4BY6}.
HELIX 123 134 {ECO:0000244|PDB:4BY6}.
STRAND 138 140 {ECO:0000244|PDB:4BY6}.
TURN 141 144 {ECO:0000244|PDB:4BY6}.
STRAND 145 149 {ECO:0000244|PDB:4BY6}.
STRAND 161 172 {ECO:0000244|PDB:4BY6}.
TURN 173 176 {ECO:0000244|PDB:4BY6}.
STRAND 177 186 {ECO:0000244|PDB:4BY6}.
HELIX 187 189 {ECO:0000244|PDB:4BY6}.
SEQUENCE 191 AA; 22363 MW; 7D0B69657F791371 CRC64;
MEKFGLKALV PLLKLEDKEL SSTYDHSMTL GADLSSMLYS LGIPRDSQDH RVLDTFQSPW
AETSRSEVEP RFFTPESFTN IPGVLQSTVT PPCFNSIQND QQRVALFQDE TLFFLFYKHP
GTVIQELTYL ELRKRNWRYH KTLKAWLTKD PMMEPIVSAD GLSERGSYVF FDPQRWEKCQ
RDFLLFYNAI M


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