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Gibberellin 3-beta-dioxygenase 1 (EC 1.14.11.15) (GA 3-oxidase 1) (Gibberellin 3 beta-hydroxylase 1)

 G3OX_PEA                Reviewed;         374 AA.
O24648; O22377; O24623; O24627;
19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
01-JAN-1998, sequence version 1.
22-NOV-2017, entry version 70.
RecName: Full=Gibberellin 3-beta-dioxygenase 1;
EC=1.14.11.15;
AltName: Full=GA 3-oxidase 1;
AltName: Full=Gibberellin 3 beta-hydroxylase 1;
Name=LE;
Pisum sativum (Garden pea).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae;
Fabeae; Pisum.
NCBI_TaxID=3888;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC
ACTIVITY, AND MUTAGENESIS OF ALA-229.
PubMed=9286112; DOI=10.1105/tpc.9.8.1435;
Lester D.R., Ross J.J., Davies P.J., Reid J.B.;
"Mendel's stem length gene (Le) encodes a gibberellin 3 beta-
hydroxylase.";
Plant Cell 9:1435-1443(1997).
[2]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY,
BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, TISSUE
SPECIFICITY, MUTAGENESIS OF LEU-89; ALA-229 AND HIS-276, AND
DISRUPTION PHENOTYPE.
PubMed=9238076; DOI=10.1073/pnas.94.16.8907;
Martin D.N., Proebsting W.M., Hedden P.;
"Mendel's dwarfing gene: cDNAs from the Le alleles and function of the
expressed proteins.";
Proc. Natl. Acad. Sci. U.S.A. 94:8907-8911(1997).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF HIS-276,
FUNCTION, CATALYTIC ACTIVITY, AND TISSUE SPECIFICITY.
Lester D.R., Mackenzie-Hose A.K., Davies P.J., Ross J.J., Reid J.B.;
"The influence of the null le-2 mutation on gibberellin levels in
developing pea seeds.";
Plant Growth Regul. 27:83-89(1999).
-!- FUNCTION: Converts the inactive gibbberellin (GA) precursors GA9
and GA20 in the bioactives gibberellins GA4 and GA1. Has a small
activity on GA29, producing GA8. Unable to convert GA20 to GA5,
GA5 to GA3 or GA12 to GA14. Involved in the production of
bioactive GA for vegetative growth and development, but not for
the 3-beta-hydroxylation of GA in developing seeds.
{ECO:0000269|PubMed:9238076, ECO:0000269|PubMed:9286112,
ECO:0000269|Ref.3}.
-!- CATALYTIC ACTIVITY: Gibberellin 20 + 2-oxoglutarate + O(2) =
gibberellin 1 + succinate + CO(2). {ECO:0000269|PubMed:9238076,
ECO:0000269|PubMed:9286112, ECO:0000269|Ref.3}.
-!- COFACTOR:
Name=L-ascorbate; Xref=ChEBI:CHEBI:38290; Evidence={ECO:0000250};
-!- COFACTOR:
Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
Evidence={ECO:0000255|PROSITE-ProRule:PRU00805};
Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
ProRule:PRU00805};
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=1.5 uM for GA9 {ECO:0000269|PubMed:9238076};
KM=13 uM for GA20 {ECO:0000269|PubMed:9238076};
Vmax=0.29 nmol/min/mg enzyme with GA9 as substrate
{ECO:0000269|PubMed:9238076};
Vmax=0.10 nmol/min/mg enzyme with GA20 as substrate
{ECO:0000269|PubMed:9238076};
-!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
-!- TISSUE SPECIFICITY: Expressed in radicles, roots, internodes,
cotyledons, leaves and shoots. Barely detected in developing
seeds. Not detected in flowers or young fruits.
{ECO:0000269|PubMed:9238076, ECO:0000269|Ref.3}.
-!- DEVELOPMENTAL STAGE: Highly expressed in germinating seedlings.
{ECO:0000269|PubMed:9238076}.
-!- DISRUPTION PHENOTYPE: Dwarf phenotype.
{ECO:0000269|PubMed:9238076}.
-!- MISCELLANEOUS: The Le gene controls the stem length trait studied
by Gregor Mendel in 1866 (PubMed:9286112 and PubMed:9238076).
-!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
family. GA3OX subfamily. {ECO:0000305}.
-!- WEB RESOURCE: Name=Protein Spotlight; Note=On the garden pea
- Issue 159 of April 2014;
URL="https://web.expasy.org/spotlight/back_issues/159/";
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EMBL; U85045; AAB65829.1; -; mRNA.
EMBL; AF001219; AAC49792.1; -; mRNA.
EMBL; AF010167; AAC49793.1; -; mRNA.
EMBL; AF010168; AAC49794.1; -; mRNA.
EMBL; U93210; AAC86820.1; -; Genomic_DNA.
EMBL; AF007766; AAC96017.1; -; mRNA.
EMBL; AF004730; AAC96015.1; -; Genomic_DNA.
PIR; T06244; T06244.
PIR; T06245; T06245.
ProteinModelPortal; O24648; -.
SMR; O24648; -.
UniPathway; UPA00390; -.
GO; GO:0016707; F:gibberellin 3-beta-dioxygenase activity; IDA:UniProtKB.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0009686; P:gibberellin biosynthetic process; IDA:UniProtKB.
Gene3D; 2.60.120.330; -; 1.
InterPro; IPR026992; DIOX_N.
InterPro; IPR027443; IPNS-like.
InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
Pfam; PF03171; 2OG-FeII_Oxy; 1.
Pfam; PF14226; DIOX_N; 1.
PROSITE; PS51471; FE2OG_OXY; 1.
1: Evidence at protein level;
Dioxygenase; Iron; Metal-binding; Oxidoreductase.
CHAIN 1 374 Gibberellin 3-beta-dioxygenase 1.
/FTId=PRO_0000425239.
DOMAIN 206 307 Fe2OG dioxygenase. {ECO:0000255|PROSITE-
ProRule:PRU00805}.
ACT_SITE 298 298 {ECO:0000255}.
METAL 231 231 Iron. {ECO:0000255|PROSITE-
ProRule:PRU00805}.
METAL 233 233 Iron. {ECO:0000255|PROSITE-
ProRule:PRU00805}.
METAL 288 288 Iron. {ECO:0000255|PROSITE-
ProRule:PRU00805}.
BINDING 298 298 2-oxoglutarate. {ECO:0000255|PROSITE-
ProRule:PRU00805}.
MUTAGEN 89 89 L->F: Strongly decreased catalytic
activity producing a dwarf phenotype;
when associated with Y-276.
{ECO:0000269|PubMed:9238076}.
MUTAGEN 229 229 A->T: In le-1; strongly decreased
catalytic activity producing a dwarf
phenotype. {ECO:0000269|PubMed:9238076,
ECO:0000269|PubMed:9286112}.
MUTAGEN 276 276 H->Y: In le-3; strongly decreased
catalytic activity producing a dwarf
phenotype. Strongly decreased catalytic
activity producing a dwarf phenotype;
when associated with F-89.
{ECO:0000269|PubMed:9238076,
ECO:0000269|Ref.3}.
SEQUENCE 374 AA; 41726 MW; B7203F5FB264C9C9 CRC64;
MPSLSEAYRA HPVHVNHKHP DFNSLQELPE SYNWTHLDDH TLIDSNNIMK ESTTTVPVID
LNDPNASKLI GLACKTWGVY QVMNHGIPLS LLEDIQWLGQ TLFSLPSHQK HKATRSPDGV
SGYGIARISS FFPKLMWYEG FTIVGSPLDH FRELWPQDYT RFCDIVVQYD ETMKKLAGTL
MCLMLDSLGI TKEDIKWAGS KAQFEKACAA LQLNSYPSCP DPDHAMGLAP HTDSTFLTIL
SQNDISGLQV NREGSGWITV PPLQGGLVVN VGDLFHILSN GLYPSVLHRV LVNRTRQRFS
VAYLYGPPSN VEICPHAKLI GPTKPPLYRS VTWNEYLGTK AKHFNKALSS VRLCTPINGL
FDVNDSNKNS VQVG


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