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Glutamine synthetase cytosolic isozyme 1-2 (EC 6.3.1.2) (Glutamate--ammonia ligase GLN1;2) (OsGLN1;2) (Glutamine synthetase root isozyme) (OsGS1;2)

 GLN12_ORYSJ             Reviewed;         357 AA.
P14654; Q10PS4; Q5NU22; Q8GTZ3;
01-APR-1990, integrated into UniProtKB/Swiss-Prot.
01-APR-1990, sequence version 1.
25-OCT-2017, entry version 118.
RecName: Full=Glutamine synthetase cytosolic isozyme 1-2;
EC=6.3.1.2;
AltName: Full=Glutamate--ammonia ligase GLN1;2;
Short=OsGLN1;2;
AltName: Full=Glutamine synthetase root isozyme;
AltName: Full=OsGS1;2;
Name=GLN1-2; Synonyms=GSR, RGS8;
OrderedLocusNames=Os03g0223400, LOC_Os03g12290;
ORFNames=OJ1743A09.19, OsJ_09974 {ECO:0000312|EMBL:EEE58620.1};
Oryza sativa subsp. japonica (Rice).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP clade;
Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
NCBI_TaxID=39947;
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Kinmaze; TISSUE=Root;
PubMed=2577497; DOI=10.1007/BF00027323;
Sakamoto A., Ogawa M., Masumura T., Shibata D., Takeba G., Tanaka K.,
Fujii S.;
"Three cDNA sequences coding for glutamine synthetase polypeptides in
Oryza sativa L.";
Plant Mol. Biol. 13:611-614(1989).
[2]
NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE
SPECIFICITY, AND INDUCTION.
STRAIN=cv. Sasanishiki; TISSUE=Root;
PubMed=15574840; DOI=10.1093/pcp/pch190;
Ishiyama K., Inoue E., Tabuchi M., Yamaya T., Takahashi H.;
"Biochemical background and compartmentalized functions of cytosolic
glutamine synthetase for active ammonium assimilation in rice roots.";
Plant Cell Physiol. 45:1640-1647(2004).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
PubMed=16109971; DOI=10.1101/gr.3869505;
The rice chromosome 3 sequencing consortium;
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R.,
Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L.,
Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S., Johri S.,
Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S.,
Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J.,
de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H.,
Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F.,
Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A.,
Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J.,
Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L.,
Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A.,
Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W.,
Lee H.R., Jiang J., Jackson S.;
"Sequence, annotation, and analysis of synteny between rice chromosome
3 and diverged grass species.";
Genome Res. 15:1284-1291(2005).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
PubMed=16100779; DOI=10.1038/nature03895;
International rice genome sequencing project (IRGSP);
"The map-based sequence of the rice genome.";
Nature 436:793-800(2005).
[5]
GENOME REANNOTATION.
STRAIN=cv. Nipponbare;
PubMed=18089549; DOI=10.1093/nar/gkm978;
The rice annotation project (RAP);
"The rice annotation project database (RAP-DB): 2008 update.";
Nucleic Acids Res. 36:D1028-D1033(2008).
[6]
GENOME REANNOTATION.
STRAIN=cv. Nipponbare;
PubMed=24280374; DOI=10.1186/1939-8433-6-4;
Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
Buell C.R., Matsumoto T.;
"Improvement of the Oryza sativa Nipponbare reference genome using
next generation sequence and optical map data.";
Rice 6:4-4(2013).
[7]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H.,
Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J.,
Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X.,
Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y.,
Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J.,
Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y.,
Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y.,
Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z.,
Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T.,
Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H.,
Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W.,
Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L.,
Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J.,
Samudrala R., Wang J., Wong G.K.-S., Yang H.;
"The genomes of Oryza sativa: a history of duplications.";
PLoS Biol. 3:266-281(2005).
[8]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Nipponbare;
The rice full-length cDNA consortium;
"Oryza sativa full length cDNA.";
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
[9]
TISSUE SPECIFICITY.
PubMed=15918879; DOI=10.1111/j.1365-313X.2005.02406.x;
Tabuchi M., Sugiyama K., Ishiyama K., Inoue E., Sato T., Takahashi H.,
Yamaya T.;
"Severe reduction in growth rate and grain filling of rice mutants
lacking OsGS1;1, a cytosolic glutamine synthetase1;1.";
Plant J. 42:641-651(2005).
-!- FUNCTION: High-affinity glutamine synthetase. May play a role in
the primary assimilation of ammonium taken up by roots.
-!- CATALYTIC ACTIVITY: ATP + L-glutamate + NH(3) = ADP + phosphate +
L-glutamine.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=2.1 mM for glutamate {ECO:0000269|PubMed:15574840};
KM=73 uM for ammonium {ECO:0000269|PubMed:15574840};
KM=530 uM for ATP {ECO:0000269|PubMed:15574840};
Vmax=94.7 nmol/sec/mg enzyme with glutamate as substrate
{ECO:0000269|PubMed:15574840};
Vmax=98.1 nmol/sec/mg enzyme with ammonium as substrate
{ECO:0000269|PubMed:15574840};
Vmax=109.1 nmol/sec/mg enzyme with ATP as substrate
{ECO:0000269|PubMed:15574840};
Note=Measured at pH 7.8 and 30 degrees Celsius for all
experiments.;
-!- SUBUNIT: Homooctamer. {ECO:0000250}.
-!- SUBCELLULAR LOCATION: Cytoplasm.
-!- TISSUE SPECIFICITY: Expressed in roots and at lower levels in leaf
blades and spikelets (rice flower). {ECO:0000269|PubMed:15574840,
ECO:0000269|PubMed:15918879}.
-!- INDUCTION: By ammonium supply under nitrogen-limited condition.
{ECO:0000269|PubMed:15574840}.
-!- SIMILARITY: Belongs to the glutamine synthetase family.
{ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=AAN05339.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
-----------------------------------------------------------------------
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EMBL; X14244; CAA32460.1; -; mRNA.
EMBL; AB180688; BAD77931.1; -; mRNA.
EMBL; AC105364; AAN05339.1; ALT_SEQ; Genomic_DNA.
EMBL; DP000009; ABF94717.1; -; Genomic_DNA.
EMBL; AP008209; BAF11338.1; -; Genomic_DNA.
EMBL; AP014959; BAS83037.1; -; Genomic_DNA.
EMBL; CM000140; EEE58620.1; -; Genomic_DNA.
EMBL; AK243037; BAH01417.1; -; mRNA.
PIR; S07469; AJRZQB.
RefSeq; XP_015631679.1; XM_015776193.1.
UniGene; Os.12728; -.
ProteinModelPortal; P14654; -.
SMR; P14654; -.
STRING; 39947.LOC_Os03g12290.1; -.
PaxDb; P14654; -.
PRIDE; P14654; -.
EnsemblPlants; OS03T0223400-01; OS03T0223400-01; OS03G0223400.
GeneID; 4332108; -.
Gramene; OS03T0223400-01; OS03T0223400-01; OS03G0223400.
KEGG; osa:4332108; -.
eggNOG; KOG0683; Eukaryota.
eggNOG; COG0174; LUCA.
HOGENOM; HOG000061500; -.
InParanoid; P14654; -.
KO; K01915; -.
OMA; WPENGFP; -.
OrthoDB; EOG09360CB0; -.
BRENDA; 6.3.1.2; 4460.
Reactome; R-OSA-210455; Astrocytic Glutamate-Glutamine Uptake And Metabolism.
Reactome; R-OSA-70614; Amino acid synthesis and interconversion (transamination).
SABIO-RK; P14654; -.
Proteomes; UP000059680; Chromosome 3.
Genevisible; P14654; OS.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0004356; F:glutamate-ammonia ligase activity; IEA:UniProtKB-EC.
GO; GO:0006542; P:glutamine biosynthetic process; IEA:InterPro.
Gene3D; 3.10.20.70; -; 1.
Gene3D; 3.30.590.10; -; 1.
InterPro; IPR008147; Gln_synt_b-grasp.
InterPro; IPR036651; Gln_synt_N.
InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
InterPro; IPR008146; Gln_synth_cat_dom.
InterPro; IPR027303; Gln_synth_gly_rich_site.
InterPro; IPR027302; Gln_synth_N_conserv_site.
Pfam; PF00120; Gln-synt_C; 1.
Pfam; PF03951; Gln-synt_N; 1.
SMART; SM01230; Gln-synt_C; 1.
SUPFAM; SSF54368; SSF54368; 1.
SUPFAM; SSF55931; SSF55931; 1.
PROSITE; PS00180; GLNA_1; 1.
PROSITE; PS00181; GLNA_ATP; 1.
1: Evidence at protein level;
ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding;
Reference proteome.
CHAIN 1 357 Glutamine synthetase cytosolic isozyme 1-
2.
/FTId=PRO_0000153186.
SEQUENCE 357 AA; 39258 MW; 2FF5634EDEF2E77E CRC64;
MANLTDLVNL NLSDCSDKII AEYIWVGGSG IDLRSKARTV KGPITDVSQL PKWNYDGSST
GQAPGEDSEV ILYPQAIFKD PFRRGDNILV MCDCYTPQGE PIPTNKRHSA AKIFSHPDVV
AEVPWYGIEQ EYTLLQKDVN WPLGWPVGGF PGPQGPYYCA AGAEKAFGRD IVDAHYKACI
YAGINISGIN GEVMPGQWEF QVGPSVGIAA ADQVWVARYI LERVTEVAGV VLSLDPKPIP
GDWNGAGAHT NFSTKSMREP GGYEVIKKAI DKLALRHKEH IAAYGEGNER RLTGRHETAD
INTFKWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTGMIAET TLLWKQN


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