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Heparan-sulfate 6-O-sulfotransferase 3-B (HS 6-OST-3-B) (EC 2.8.2.-)

 H6S3B_DANRE             Reviewed;         419 AA.
A0MGZ7;
17-APR-2007, integrated into UniProtKB/Swiss-Prot.
12-DEC-2006, sequence version 1.
05-DEC-2018, entry version 63.
RecName: Full=Heparan-sulfate 6-O-sulfotransferase 3-B {ECO:0000312|ZFIN:ZDB-GENE-070103-3};
Short=HS 6-OST-3-B {ECO:0000305};
EC=2.8.2.- {ECO:0000269|PubMed:28103688};
Name=hs6st3b {ECO:0000312|ZFIN:ZDB-GENE-070103-3};
Danio rerio (Zebrafish) (Brachydanio rerio).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
Cyprinidae; Danio.
NCBI_TaxID=7955;
[1] {ECO:0000305, ECO:0000312|EMBL:ABH11458.1}
NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL
STAGE.
PubMed=17075883; DOI=10.1002/dvdy.20990;
Cadwallader A.B., Yost H.J.;
"Combinatorial expression patterns of heparan sulfate
sulfotransferases in zebrafish: II. The 6-O-sulfotransferase family.";
Dev. Dyn. 235:3432-3437(2006).
[2]
X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 75-395 IN COMPLEX WITH PAPS
AND HEPARAN SULFATE OLIGOSACCHARIDE SUBSTRATES, FUNCTION, CATALYTIC
ACTIVITY, ACTIVE SITE, AND MUTAGENESIS OF HIS-101; LYS-104; LYS-131;
LYS-132; ARG-148; TRP-153; HIS-158; HIS-203 AND TRP-210.
PubMed=28103688; DOI=10.1021/acschembio.6b00841;
Xu Y., Moon A.F., Xu S., Krahn J.M., Liu J., Pedersen L.C.;
"Structure based substrate specificity analysis of heparan sulfate 6-
O-sulfotransferases.";
ACS Chem. Biol. 12:73-82(2017).
-!- FUNCTION: 6-O-sulfation enzyme which catalyzes the transfer of
sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to
position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan
sulfate. {ECO:0000269|PubMed:28103688}.
-!- CATALYTIC ACTIVITY:
Reaction=3'-phosphoadenylyl sulfate + alpha-D-glucosaminyl-
[heparan sulfate](n) = 6-sulfo-alpha-D-glucosaminyl-[heparan
sulfate](n) + adenosine 3',5'-bisphosphate + H(+);
Xref=Rhea:RHEA:56604, Rhea:RHEA-COMP:9830, Rhea:RHEA-COMP:14621,
ChEBI:CHEBI:15378, ChEBI:CHEBI:58339, ChEBI:CHEBI:58343,
ChEBI:CHEBI:58388, ChEBI:CHEBI:140604;
Evidence={ECO:0000269|PubMed:28103688};
-!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass type II
membrane protein {ECO:0000255}.
-!- TISSUE SPECIFICITY: In early somitogenesis, expressed in
presumptive forebrain and midbrain, tail bud and Kupffer's
vesicle. During mid-somitogenesis, ubiquitous expression which is
strongest in the somites and eye. During late somitogenesis,
predominantly expressed in eye, hindbrain and ventral somites. At
24 hours post-fertilization (hpf), restricted to lens and neural
retina, brain, otic vesicle and somites. At 36 hpf, brain
expression is restricted to telencephalon. At 48 hpf, restricted
to telencephalon and pectoral fin. {ECO:0000269|PubMed:17075883}.
-!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
{ECO:0000269|PubMed:17075883}.
-!- SIMILARITY: Belongs to the sulfotransferase 6 family.
{ECO:0000255}.
-----------------------------------------------------------------------
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EMBL; DQ812996; ABH11458.1; -; mRNA.
RefSeq; NP_001073663.1; NM_001080194.1.
UniGene; Dr.110384; -.
PDB; 5T03; X-ray; 2.10 A; A/B=75-395.
PDB; 5T05; X-ray; 1.95 A; A/B=75-395.
PDB; 5T0A; X-ray; 1.95 A; A/B=75-395.
PDBsum; 5T03; -.
PDBsum; 5T05; -.
PDBsum; 5T0A; -.
SMR; A0MGZ7; -.
STRING; 7955.ENSDARP00000065928; -.
PaxDb; A0MGZ7; -.
GeneID; 569353; -.
KEGG; dre:569353; -.
CTD; 569353; -.
ZFIN; ZDB-GENE-070103-3; hs6st3b.
eggNOG; KOG3955; Eukaryota.
eggNOG; ENOG410XSW4; LUCA.
HOGENOM; HOG000007772; -.
HOVERGEN; HBG083012; -.
InParanoid; A0MGZ7; -.
KO; K08103; -.
PRO; PR:A0MGZ7; -.
Proteomes; UP000000437; Unplaced.
GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
GO; GO:0050656; F:3'-phosphoadenosine 5'-phosphosulfate binding; IDA:ZFIN.
GO; GO:0017095; F:heparan sulfate 6-O-sulfotransferase activity; IBA:GO_Central.
GO; GO:0070492; F:oligosaccharide binding; IDA:ZFIN.
GO; GO:0008146; F:sulfotransferase activity; IDA:ZFIN.
GO; GO:0015015; P:heparan sulfate proteoglycan biosynthetic process, enzymatic modification; IBA:GO_Central.
InterPro; IPR010635; Heparan_SO4-6-sulfoTrfase.
InterPro; IPR027417; P-loop_NTPase.
InterPro; IPR005331; Sulfotransferase.
PANTHER; PTHR12812; PTHR12812; 1.
Pfam; PF03567; Sulfotransfer_2; 1.
SUPFAM; SSF52540; SSF52540; 1.
1: Evidence at protein level;
3D-structure; Complete proteome; Glycoprotein; Membrane;
Reference proteome; Signal-anchor; Transferase; Transmembrane;
Transmembrane helix.
CHAIN 1 419 Heparan-sulfate 6-O-sulfotransferase 3-B.
/FTId=PRO_0000283820.
TOPO_DOM 1 7 Cytoplasmic. {ECO:0000255}.
TRANSMEM 8 28 Helical; Signal-anchor for type II
membrane protein. {ECO:0000255}.
TOPO_DOM 29 419 Lumenal. {ECO:0000255}.
REGION 101 109 PAPS binding.
{ECO:0000269|PubMed:28103688}.
REGION 131 132 Substrate binding.
{ECO:0000269|PubMed:28103688}.
REGION 323 325 PAPS binding.
{ECO:0000269|PubMed:28103688}.
REGION 329 330 PAPS binding.
{ECO:0000269|PubMed:28103688}.
ACT_SITE 158 158 Proton acceptor.
{ECO:0000305|PubMed:28103688}.
BINDING 148 148 Substrate. {ECO:0000269|PubMed:28103688}.
BINDING 153 153 Substrate. {ECO:0000269|PubMed:28103688}.
BINDING 158 158 Substrate. {ECO:0000269|PubMed:28103688}.
BINDING 191 191 PAPS. {ECO:0000269|PubMed:28103688}.
BINDING 199 199 PAPS. {ECO:0000269|PubMed:28103688}.
BINDING 203 203 Substrate. {ECO:0000269|PubMed:28103688}.
BINDING 210 210 Substrate. {ECO:0000269|PubMed:28103688}.
CARBOHYD 77 77 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
CARBOHYD 270 270 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
CARBOHYD 275 275 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
CARBOHYD 326 326 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
CARBOHYD 393 393 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
CARBOHYD 402 402 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
MUTAGEN 101 101 H->A: Significantly impairs catalytic
activity. {ECO:0000269|PubMed:28103688}.
MUTAGEN 104 104 K->A: Significantly impairs catalytic
activity. {ECO:0000269|PubMed:28103688}.
MUTAGEN 131 131 K->A: Slightly impairs catalytic
activity. {ECO:0000269|PubMed:28103688}.
MUTAGEN 132 132 K->A,E: Significantly impairs catalytic
activity. {ECO:0000269|PubMed:28103688}.
MUTAGEN 148 148 R->A: Moderately impairs catalytic
activity. {ECO:0000269|PubMed:28103688}.
MUTAGEN 153 153 W->A: Moderately impairs catalytic
activity. {ECO:0000269|PubMed:28103688}.
MUTAGEN 158 158 H->A: Abolishes catalytic activity.
{ECO:0000269|PubMed:28103688}.
MUTAGEN 203 203 H->A: Significantly impairs catalytic
activity. {ECO:0000269|PubMed:28103688}.
MUTAGEN 210 210 W->A: Significantly impairs catalytic
activity. {ECO:0000269|PubMed:28103688}.
HELIX 80 83 {ECO:0000244|PDB:5T0A}.
TURN 91 94 {ECO:0000244|PDB:5T0A}.
STRAND 96 99 {ECO:0000244|PDB:5T0A}.
HELIX 107 116 {ECO:0000244|PDB:5T0A}.
STRAND 143 147 {ECO:0000244|PDB:5T0A}.
TURN 148 151 {ECO:0000244|PDB:5T0A}.
HELIX 161 172 {ECO:0000244|PDB:5T0A}.
STRAND 184 190 {ECO:0000244|PDB:5T0A}.
HELIX 193 205 {ECO:0000244|PDB:5T0A}.
TURN 224 226 {ECO:0000244|PDB:5T0A}.
STRAND 230 234 {ECO:0000244|PDB:5T05}.
HELIX 241 246 {ECO:0000244|PDB:5T0A}.
TURN 251 254 {ECO:0000244|PDB:5T0A}.
HELIX 255 260 {ECO:0000244|PDB:5T0A}.
HELIX 263 266 {ECO:0000244|PDB:5T0A}.
TURN 267 269 {ECO:0000244|PDB:5T0A}.
HELIX 276 293 {ECO:0000244|PDB:5T0A}.
STRAND 294 299 {ECO:0000244|PDB:5T0A}.
HELIX 303 314 {ECO:0000244|PDB:5T0A}.
STRAND 318 320 {ECO:0000244|PDB:5T0A}.
TURN 329 332 {ECO:0000244|PDB:5T0A}.
HELIX 337 346 {ECO:0000244|PDB:5T0A}.
HELIX 348 382 {ECO:0000244|PDB:5T0A}.
HELIX 384 389 {ECO:0000244|PDB:5T0A}.
SEQUENCE 419 AA; 49346 MW; D83CDCF7356749B5 CRC64;
MNDKPNKWIF IPILAILFVM IGYQYVCPAG GQACHFRTGD KLVRIAPLAT PDPTTDDLYR
EQDPEEDNPP KCASKFNFTE RDLTRDVDFN IKGDDVIVFL HIQKTGGTTF GRHLVRNIRL
EQPCDCKAGQ KKCTCHRPGK QESWLFSRFS TGWSCGLHAD WTELTNCVPV IMDKRQPPKR
KRNFYYITML RDPVSRYLSE WKHVQRGATW KTSLHMCDGR SPTQDELPTC YNGDDWSGVT
LHDFMDCPSN LANNRQVRML ADLSLVGCYN LSTMNESERN PILLASAKSN LKNMAFYGLT
EFQRKTQYLF ERTFHLRFIS AFTQINSTRA ANVELRDDMR SRIEQLNMLD MQLYEFAKDL
FLQRYQFVRQ RERQEERLKR REERRWIRER RVNQSKEPIV ENQTRVTTTE DYASQVVRW


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