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High-molecular weight cobalt-containing nitrile hydratase subunit alpha (H-NHase) (H-nitrilase) (EC 4.2.1.84)

 NHA1_RHORH              Reviewed;         203 AA.
P21219;
01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
23-JAN-2007, sequence version 3.
25-OCT-2017, entry version 86.
RecName: Full=High-molecular weight cobalt-containing nitrile hydratase subunit alpha;
Short=H-NHase;
Short=H-nitrilase;
EC=4.2.1.84;
Name=nhhA;
Rhodococcus rhodochrous.
Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae;
Rhodococcus.
NCBI_TaxID=1829;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-27 AND
162-173.
STRAIN=J1;
PubMed=1840499; DOI=10.1016/0167-4781(91)90208-4;
Kobayashi M., Nishiyama M., Nagasawa T., Horinouchi S., Beppu T.,
Yamada H.;
"Cloning, nucleotide sequence and expression in Escherichia coli of
two cobalt-containing nitrile hydratase genes from Rhodococcus
rhodochrous J1.";
Biochim. Biophys. Acta 1129:23-33(1991).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=J1;
PubMed=8633053; DOI=10.1073/pnas.93.9.4267;
Komeda H., Kobayashi M., Shimizu S.;
"Characterization of the gene cluster of high-molecular-mass nitrile
hydratase (H-NHase) induced by its reaction product in Rhodococcus
rhodochrous J1.";
Proc. Natl. Acad. Sci. U.S.A. 93:4267-4272(1996).
[3]
PROTEIN SEQUENCE OF 2-29.
STRAIN=J1;
PubMed=2013281; DOI=10.1111/j.1432-1033.1991.tb15853.x;
Nagasawa T., Takeuchi K., Yamada H.;
"Characterization of a new cobalt-containing nitrile hydratase
purified from urea-induced cells of Rhodococcus rhodochrous J1.";
Eur. J. Biochem. 196:581-589(1991).
-!- FUNCTION: NHase catalyzes the hydration of various nitrile
compounds to the corresponding amides.
-!- CATALYTIC ACTIVITY: An aliphatic amide = a nitrile + H(2)O.
-!- COFACTOR:
Name=Co(3+); Xref=ChEBI:CHEBI:49415;
Note=Binds 1 Co(3+) ion per subunit.;
-!- SUBUNIT: Heterodimer of an alpha and a beta chain.
-!- INDUCTION: By cobalt and urea or cyclohexanecarboxamide.
-!- BIOTECHNOLOGY: Industrial production of acrylamide is now being
developed using some of these enzymes.
-!- SIMILARITY: Belongs to the nitrile hydratase subunit alpha family.
{ECO:0000305}.
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EMBL; X64359; CAA45710.1; -; Genomic_DNA.
EMBL; D67027; BAA11044.1; -; Genomic_DNA.
PIR; S19714; S19714.
ProteinModelPortal; P21219; -.
SMR; P21219; -.
PRIDE; P21219; -.
BioCyc; MetaCyc:MONOMER-2283; -.
GO; GO:0080109; F:indole-3-acetonitrile nitrile hydratase activity; IEA:UniProtKB-EC.
GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
Gene3D; 3.90.330.10; -; 1.
InterPro; IPR036648; CN_Hdrase_a/SCN_Hdrase_g_sf.
InterPro; IPR004232; CN_Hdrtase_a/SCN_Hdrlase_g.
InterPro; IPR023900; CN_Hdrtase_asu/SCN_Hdrlase_gsu.
InterPro; IPR018141; Nitrile_hydratase_asu.
Pfam; PF02979; NHase_alpha; 1.
PIRSF; PIRSF001426; NHase_alpha; 1.
ProDom; PD007559; CN_Hdrtase_asu/SCN_Hdrlase_gsu; 1.
SUPFAM; SSF56209; SSF56209; 1.
TIGRFAMs; TIGR01323; nitrile_alph; 1.
1: Evidence at protein level;
Cobalt; Direct protein sequencing; Lyase; Metal-binding.
INIT_MET 1 1 Removed. {ECO:0000269|PubMed:1840499,
ECO:0000269|PubMed:2013281}.
CHAIN 2 203 High-molecular weight cobalt-containing
nitrile hydratase subunit alpha.
/FTId=PRO_0000186824.
METAL 102 102 Cobalt. {ECO:0000250}.
METAL 105 105 Cobalt. {ECO:0000250}.
METAL 106 106 Cobalt. {ECO:0000250}.
METAL 107 107 Cobalt. {ECO:0000250}.
SEQUENCE 203 AA; 22835 MW; 515ED2A4863E6C98 CRC64;
MSEHVNKYTE YEARTKAIET LLYERGLITP AAVDRVVSYY ENEIGPMGGA KVVAKSWVDP
EYRKWLEEDA TAAMASLGYA GEQAHQISAV FNDSQTHHVV VCTLCSCYPW PVLGLPPAWY
KSMEYRSRVV ADPRGVLKRD FGFDIPDEVE VRVWDSSSEI RYIVIPERPA GTDGWSEEEL
TKLVSRDSMI GVSNALTPQE VIV


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