Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Holin (Lysis protein) (Protein rV)

 HOLIN_BPT4              Reviewed;         218 AA.
P06808;
01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
01-JAN-1988, sequence version 1.
10-OCT-2018, entry version 81.
RecName: Full=Holin {ECO:0000255|HAMAP-Rule:MF_04104};
AltName: Full=Lysis protein;
AltName: Full=Protein rV;
Name=t; Synonyms=rV;
Enterobacteria phage T4 (Bacteriophage T4).
Viruses; dsDNA viruses, no RNA stage; Caudovirales; Myoviridae;
Tevenvirinae; T4virus.
NCBI_TaxID=10665;
NCBI_TaxID=562; Escherichia coli.
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3628006; DOI=10.1093/nar/15.16.6736;
Montag D., Degen M., Henning U.;
"Nucleotide sequence of gene t (lysis gene) of the E. coli phage T4.";
Nucleic Acids Res. 15:6736-6736(1987).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
PubMed=12626685; DOI=10.1128/MMBR.67.1.86-156.2003;
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
"Bacteriophage T4 genome.";
Microbiol. Mol. Biol. Rev. 67:86-156(2003).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34.
PubMed=2958637; DOI=10.1016/0022-2836(87)90519-5;
Montag D., Riede I., Eschbach M.-L., Degen M., Henning U.;
"Receptor-recognizing proteins of T-even type bacteriophages. Constant
and hypervariable regions and an unusual case of evolution.";
J. Mol. Biol. 196:165-174(1987).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-218.
STRAIN=D;
PubMed=8416914; DOI=10.1128/jb.175.1.85-93.1993;
Orsini G., Ouhammouch M., Le Caer J.-P., Brody E.N.;
"The asiA gene of bacteriophage T4 codes for the anti-sigma 70
protein.";
J. Bacteriol. 175:85-93(1993).
[5]
FUNCTION, AND TOPOLOGY.
PubMed=11255004; DOI=10.1016/S0378-1119(01)00365-1;
Ramanculov E., Young R.;
"Genetic analysis of the T4 holin: timing and topology.";
Gene 265:25-36(2001).
[6]
INTERACTION WITH HOLIN, AND SUBCELLULAR LOCATION.
PubMed=16166524; DOI=10.1128/JB.187.19.6631-6640.2005;
Tran T.A., Struck D.K., Young R.;
"Periplasmic domains define holin-antiholin interactions in t4 lysis
inhibition.";
J. Bacteriol. 187:6631-6640(2005).
[7]
FUNCTION, MUTAGENESIS OF CYS-175 AND CYS-207, AND DISULFIDE BOND.
PubMed=22389108; DOI=10.1002/pro.2042;
Moussa S.H., Kuznetsov V., Tran T.A., Sacchettini J.C., Young R.;
"Protein determinants of phage T4 lysis inhibition.";
Protein Sci. 21:571-582(2012).
[8]
INTERACTION WITH LYSIS INHIBITION ACCESSORY PROTEIN.
PubMed=27381920; DOI=10.1128/JB.00294-16;
Chen Y., Young R.;
"The last r locus unveiled: T4 RIII is a cytoplasmic antiholin.";
J. Bacteriol. 198:2448-2457(2016).
-!- FUNCTION: Accumulates harmlessly in the cytoplasmic membrane until
it reaches a critical concentration that triggers the formation of
micron-scale pores (holes) causing host cell membrane disruption
and endolysin escape into the periplasmic space. Determines the
precise timing of host cell lysis. Participates with the endolysin
and spanin proteins in the sequential events which lead to the
programmed host cell lysis releasing the mature viral particles
from the host cell. {ECO:0000255|HAMAP-Rule:MF_04104,
ECO:0000269|PubMed:11255004, ECO:0000269|PubMed:22389108}.
-!- SUBUNIT: Homomultimer. Interacts (via C-terminus) with antiholin
(via C-terminus); this interaction blocks the holin
homomultimerization and delays host cell lysis (PubMed:16166524).
Interacts (via N-terminus) with the lysis inhibition accessory
protein rIII; this interaction stabilizes the holin-antiholin
complex thereby resulting in a robust block of the hole formation
(PubMed:27381920). {ECO:0000255|HAMAP-Rule:MF_04104,
ECO:0000269|PubMed:16166524, ECO:0000269|PubMed:27381920}.
-!- SUBCELLULAR LOCATION: Host cell inner membrane {ECO:0000255|HAMAP-
Rule:MF_04104, ECO:0000305|PubMed:16166524}; Single-pass type II
membrane protein {ECO:0000255|HAMAP-Rule:MF_04104,
ECO:0000305|PubMed:16166524}; Periplasmic side {ECO:0000255|HAMAP-
Rule:MF_04104, ECO:0000305|PubMed:16166524}. Note=Classified as a
class III holin. {ECO:0000255|HAMAP-Rule:MF_04104}.
-!- PTM: Disulfide bond is required for functionality.
{ECO:0000255|HAMAP-Rule:MF_04104, ECO:0000269|PubMed:22389108}.
-!- SIMILARITY: Belongs to the T4likevirus holin family.
{ECO:0000255|HAMAP-Rule:MF_04104}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; Y00408; CAA68470.1; -; Genomic_DNA.
EMBL; AF158101; AAD42661.1; -; Genomic_DNA.
EMBL; X05677; CAA29164.1; -; Genomic_DNA.
EMBL; M99441; AAA32481.1; -; Genomic_DNA.
PIR; JF0028; YVBPT4.
RefSeq; NP_049865.1; NC_000866.4.
TCDB; 1.E.8.1.1; the t4 holin (t4 holin) family.
GeneID; 1258610; -.
KEGG; vg:1258610; -.
OrthoDB; VOG090000P3; -.
Proteomes; UP000009087; Genome.
GO; GO:0020002; C:host cell plasma membrane; IDA:CACAO.
GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
GO; GO:0034291; F:canonical holin activity; IGI:CACAO.
GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
HAMAP; MF_04104; HOLIN_T4; 1.
InterPro; IPR020982; Phage_T4_GpT_holin.
Pfam; PF11031; Phage_holin_T; 1.
1: Evidence at protein level;
Complete proteome; Cytolysis; Disulfide bond;
Host cell inner membrane; Host cell lysis by virus;
Host cell membrane; Host membrane; Membrane; Reference proteome;
Signal-anchor; Transmembrane; Transmembrane helix;
Viral release from host cell.
CHAIN 1 218 Holin.
/FTId=PRO_0000165066.
TOPO_DOM 1 34 Cytoplasmic. {ECO:0000255|HAMAP-
Rule:MF_04104,
ECO:0000305|PubMed:11255004}.
TRANSMEM 35 49 Helical; Signal-anchor for type II
membrane protein. {ECO:0000255|HAMAP-
Rule:MF_04104,
ECO:0000305|PubMed:11255004}.
TOPO_DOM 50 218 Periplasmic. {ECO:0000255|HAMAP-
Rule:MF_04104,
ECO:0000305|PubMed:11255004}.
DISULFID 175 207 {ECO:0000255|HAMAP-Rule:MF_04104,
ECO:0000269|PubMed:22389108}.
MUTAGEN 175 175 C->S: Complete loss of lysis.
{ECO:0000269|PubMed:22389108}.
MUTAGEN 207 207 C->S: Complete loss of lysis.
{ECO:0000269|PubMed:22389108}.
SEQUENCE 218 AA; 25176 MW; 9110BE111D772DF5 CRC64;
MAAPRISFSP SDILFGVLDR LFKDNATGKV LASRVAVVIL LFIMAIVWYR GDSFFEYYKQ
SKYETYSEII EKERTARFES VALEQLQIVH ISSEADFSAV YSFRPKNLNY FVDIIAYEGK
LPSTISEKSL GGYPVDKTMD EYTVHLNGRH YYSNSKFAFL PTKKPTPEIN YMYSCPYFNL
DNIYAGTITM YWYRNDHISN DRLESICAQA ARILGRAK


Related products :

Catalog number Product name Quantity
G032 1X Protein Lysis Buffer 5.0ml
G032 Protein Lysis Buffer 1X 1X; 5X1.0ml
G031 5X Protein Lysis Buffer 3.0ml
22040051-2 Protein pur-E.coli lysis Buffer 500 mL
22040051-1 Protein pur-E.coli lysis Buffer 250 mL
ELSP-1 ELISA Lysis and Protein Extraction Buffer
PL013-10ml Lysis-EZ G Non-denaturing Cell Lysis Buffer, 1X Strength Solution 10ml
PL015 Lysis-EZ L, White Blood Cell Lysis Buffer, 1X Strength Solution 10ml
PL013-5x10ml Lysis-EZ G Non-denaturing Cell Lysis Buffer, 1X Strength Solution 5x10ml
PL015 Lysis-EZ L, White Blood Cell Lysis Buffer, 1X Strength Solution 10x10ml
PL012 Lysis-EZ K, Red Blood Cell (RBC) Lysis Buffer, 2x Strength Solution 100ml
WSE-7420 EzRIPA Lysis kit Subcellular Fractionation Protein Analysis 100 samples, up to 2 x 10^7 cell/sample
EL-lysis Cell_Tissue Lysis Buffer (Item J) ELISA 5 mL x 2
BSP026-5x20ml Yeast Protein Lysis Buffer, 1x Strength Solution 5x20ml
BSP023-4x25ml Bacterial Protein Lysis Buffer, 1x Stength Solution 4x25ml
BSP026-20ml Yeast Protein Lysis Buffer, 1x Strength Solution 20ml
BSP023-25ml Bacterial Protein Lysis Buffer, 1x Stength Solution 25ml
BSP022-20ml Animal Cells Protein Lysis Buffer, 1x Strength Solution 20ml
BSP022-5x20ml Animal Cells Protein Lysis Buffer, 1x Strength Solution 5x20ml
RIPA-51 RIPA lysis buffer kit for mammalian cell lysis (without protease inhibitors) Species Reactivity: 50 ml Product tipe: Buffer
RIPA-51 RIPA lysis buffer kit for mammalian cell lysis (without protease inhibitors) 50 ml
BN008 Ni-NTA Denaturation Lysis buffer B, Ni-NTA protein purification-Denaturation 5x500ml
BN012 Ni-NTA Native Lysis buffer , Ni-NTA protein purification-Native 5x500ml
BN007 Ni-NTA Denaturation Lysis buffer A, Ni-NTA protein purification-Denaturation 500ml
RIPA-50 RIPA lysis buffer kit for mammalian cell lysis (complete with protease inhibitor cocktail (PMSF, Protease inhibitors, Orthovandate) Species Reactivity: 50 ml Product tipe: Buffer


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur