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Hrp pili protein HrpA (TTSS pilin HrpA)

 HRPA_PSESM              Reviewed;         113 AA.
Q52473; Q7C4I1;
07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
23-JAN-2007, sequence version 3.
07-JUN-2017, entry version 89.
RecName: Full=Hrp pili protein HrpA;
AltName: Full=TTSS pilin HrpA;
Name=hrpA; Synonyms=hrpA1; OrderedLocusNames=PSPTO_1381;
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
NCBI_TaxID=223283;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC BAA-871 / DC3000;
PubMed=7579616;
Preston G., Huang H.-C., He S.Y., Collmer A.;
"The HrpZ proteins of Pseudomonas syringae pvs. syringae, glycinea,
and tomato are encoded by an operon containing Yersinia ysc homologs
and elicit the hypersensitive response in tomato but not soybean.";
Mol. Plant Microbe Interact. 8:717-732(1995).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC BAA-871 / DC3000;
PubMed=12580281; DOI=10.1094/MPMI.2003.16.1.43;
Fouts D.E., Badel J.L., Ramos A.R., Rapp R.A., Collmer A.;
"A Pseudomonas syringae pv. tomato DC3000 Hrp (type III secretion)
deletion mutant expressing the Hrp system of bean pathogen P. syringae
pv. syringae 61 retains normal host specificity for tomato.";
Mol. Plant Microbe Interact. 16:43-52(2003).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ICMP 2844;
Inoue Y., Takikawa Y.;
"Phylogenic analysis of DNA sequences around the hrpZ regions of
Pseudomonas syringae.";
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC BAA-871 / DC3000;
PubMed=12928499; DOI=10.1073/pnas.1731982100;
Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F.,
Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H.,
Nelson W.C., Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q.,
Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J.,
Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M.,
Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K.,
Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X.,
Bender C.L., White O., Fraser C.M., Collmer A.;
"The complete genome sequence of the Arabidopsis and tomato pathogen
Pseudomonas syringae pv. tomato DC3000.";
Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
[5]
PROTEIN SEQUENCE OF 2-11; 16-25 AND 35-50, MASS SPECTROMETRY, AND
FUNCTION.
STRAIN=ATCC BAA-871 / DC3000;
PubMed=9395289; DOI=10.1016/S0014-5793(97)01276-3;
Roine E., Saarinen J., Kalkkinen N., Romantschuk M.;
"Purified HrpA of Pseudomonas syringae pv. tomato DC3000 reassembles
into pili.";
FEBS Lett. 417:168-172(1997).
[6]
PROTEIN SEQUENCE OF 2-36, AND FUNCTION.
STRAIN=ATCC BAA-871 / DC3000;
PubMed=9096416; DOI=10.1073/pnas.94.7.3459;
Roine E., Wei W., Yuan J., Nurmiaho-Lassila E.-L., Kalkkinen N.,
Romantschuk M., He S.Y.;
"Hrp pilus: an hrp-dependent bacterial surface appendage produced by
Pseudomonas syringae pv. tomato DC3000.";
Proc. Natl. Acad. Sci. U.S.A. 94:3459-3464(1997).
[7]
MUTAGENESIS.
STRAIN=ATCC BAA-871 / DC3000;
PubMed=10564513; DOI=10.1046/j.1365-2958.1999.01635.x;
Taira S., Tuimala J., Roine E., Nurmiaho-Lassila E.-L., Savilahti H.,
Romantschuk M.;
"Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene
encoding Hrp pilus subunit.";
Mol. Microbiol. 34:737-744(1999).
[8]
FUNCTION, REGULATION OF HRP-ASSOCIATED GENES, AND MUTAGENESIS OF
GLY-23; ALA-54; LYS-93; GLU-94; ASP-95; ILE-101 AND ILE-111.
STRAIN=ATCC BAA-871 / DC3000;
PubMed=10681465; DOI=10.1073/pnas.040570097;
Wei W., Plovanich-Jones A., Deng W.-L., Jin Q.-L., Collmer A.,
Huang H.-C., He S.Y.;
"The gene coding for the Hrp pilus structural protein is required for
type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv.
tomato.";
Proc. Natl. Acad. Sci. U.S.A. 97:2247-2252(2000).
[9]
FUNCTION OF HRP PILUS.
STRAIN=ATCC BAA-871 / DC3000;
PubMed=11401717; DOI=10.1046/j.1365-2958.2001.02455.x;
Jin Q.-L., Hu W., Brown I., McGhee G., Hart P., Jones A.L., He S.Y.;
"Visualization of secreted Hrp and Avr proteins along the Hrp pilus
during type III secretion in Erwinia amylovora and Pseudomonas
syringae.";
Mol. Microbiol. 40:1129-1139(2001).
[10]
FUNCTION OF HRP PILUS.
STRAIN=ATCC BAA-871 / DC3000;
PubMed=11752577; DOI=10.1126/science.1066397;
Jin Q.-L., He S.Y.;
"Role of the Hrp pilus in type III protein secretion in Pseudomonas
syringae.";
Science 294:2556-2558(2001).
[11]
SECRETION SIGNAL.
STRAIN=ATCC BAA-871 / DC3000;
PubMed=11862491; DOI=10.1007/s00438-001-0619-4;
Hienonen E., Roine E., Romantschuk M., Taira S.;
"mRNA stability and the secretion signal of HrpA, a pilin secreted by
the type III system in Pseudomonas syringae.";
Mol. Genet. Genomics 266:973-978(2002).
-!- FUNCTION: Major structural protein of the Hrp pilus, which is a
component of the type III secretion system (TTSS, Hrp secretion
system) required for effector protein delivery, parasitism, and
pathogenicity. Required for secretion of virulence proteins such
as HrpW and AvrPto. The Hrp pilus functions as a conduit for
protein delivery into the host cell. Also, affects the expression
of TTSS-associated genes. Required for full expression of genes
that encode regulatory, secretion, and effector proteins of the
TTSS. HrpA-mediated gene regulation apparently is through effect
on the mRNA level of hrpR and hrpS. {ECO:0000269|PubMed:10681465,
ECO:0000269|PubMed:11401717, ECO:0000269|PubMed:11752577,
ECO:0000269|PubMed:9096416, ECO:0000269|PubMed:9395289}.
-!- SUBCELLULAR LOCATION: Secreted, extracellular space. Fimbrium.
Note=Extracellular and secreted via type III secretion system.
-!- INDUCTION: Expression enhanced by contact with host cell wall.
Also regulated by HrpRS and HrpL.
-!- PTM: HrpA might be cleaved/degraded in 3 forms but it is not known
if the observed processing has any biological significance.
-!- MASS SPECTROMETRY: Mass=11270; Method=MALDI; Range=2-113;
Evidence={ECO:0000269|PubMed:9395289};
-!- MISCELLANEOUS: Analysis of mutants revealed that the C-terminus is
important for pilus assembly and secretion and / or regulation
function of HprA.
-!- SIMILARITY: Belongs to the HrpA type 1 family. {ECO:0000305}.
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EMBL; AF232004; AAB00126.1; -; Genomic_DNA.
EMBL; AB112567; BAD20879.1; -; Genomic_DNA.
EMBL; AE016853; AAO54903.1; -; Genomic_DNA.
RefSeq; NP_791208.1; NC_004578.1.
RefSeq; WP_005763904.1; NC_004578.1.
STRING; 223283.PSPTO_1381; -.
EnsemblBacteria; AAO54903; AAO54903; PSPTO_1381.
GeneID; 1183017; -.
KEGG; pst:PSPTO_1381; -.
PATRIC; fig|223283.9.peg.1403; -.
HOGENOM; HOG000270098; -.
OMA; SSKAMED; -.
OrthoDB; POG091H1NRL; -.
Proteomes; UP000002515; Chromosome.
GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
GO; GO:0009289; C:pilus; IEA:UniProtKB-SubCell.
GO; GO:0009405; P:pathogenesis; IEA:UniProtKB-KW.
InterPro; IPR018581; T3SS_pilus_HrpA.
Pfam; PF09589; HrpA_pilin; 1.
1: Evidence at protein level;
Complete proteome; Direct protein sequencing; Fimbrium;
Reference proteome; Secreted; Virulence.
INIT_MET 1 1 Removed. {ECO:0000269|PubMed:9096416,
ECO:0000269|PubMed:9395289}.
CHAIN 2 113 Hrp pili protein HrpA.
/FTId=PRO_0000219574.
MUTAGEN 23 23 G->A: No effect.
{ECO:0000269|PubMed:10681465}.
MUTAGEN 54 54 A->E: No effect.
{ECO:0000269|PubMed:10681465}.
MUTAGEN 93 93 K->I: No effect.
{ECO:0000269|PubMed:10681465}.
MUTAGEN 94 94 E->K: Affects the regulatory function.
{ECO:0000269|PubMed:10681465}.
MUTAGEN 95 95 D->S: Affects the secretion function.
{ECO:0000269|PubMed:10681465}.
MUTAGEN 101 101 I->T: No effect.
{ECO:0000269|PubMed:10681465}.
MUTAGEN 111 111 I->P: Affects the secretion function.
{ECO:0000269|PubMed:10681465}.
SEQUENCE 113 AA; 11387 MW; 8E6C28F26B8FB080 CRC64;
MVAFAGLTSK LTNLGNSAVG GVGGALQGVN TVASNATLQK NILLGTGDSL SVDAQAKASK
ESDANGAKLI AMQAQETMKK QTMDVLNAIQ AGKEDSTNKK ISATATNAKG ISY


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