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Indole-3-acetic acid-amido synthetase GH3.6 (EC 6.3.2.-) (Auxin-responsive GH3-like protein 6) (AtGH3-6) (Protein DWARF IN LIGHT 1) (DFL-1)

 GH36_ARATH              Reviewed;         612 AA.
Q9LSQ4; Q8VYV6;
26-APR-2005, integrated into UniProtKB/Swiss-Prot.
01-OCT-2000, sequence version 1.
23-MAY-2018, entry version 89.
RecName: Full=Indole-3-acetic acid-amido synthetase GH3.6;
EC=6.3.2.-;
AltName: Full=Auxin-responsive GH3-like protein 6;
Short=AtGH3-6;
AltName: Full=Protein DWARF IN LIGHT 1;
Short=DFL-1;
Name=GH3.6; Synonyms=DFL1; OrderedLocusNames=At5g54510;
ORFNames=F24B18.13;
Arabidopsis thaliana (Mouse-ear cress).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
Arabidopsis.
NCBI_TaxID=3702;
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION.
STRAIN=cv. Landsberg erecta;
PubMed=11169197; DOI=10.1046/j.1365-313x.2001.00957.x;
Nakazawa M., Yabe N., Ichikawa T., Yamamoto Y.Y., Yoshizumi T.,
Hasunuma K., Matsui M.;
"DFL1, an auxin-responsive GH3 gene homologue, negatively regulates
shoot cell elongation and lateral root formation, and positively
regulates the light response of hypocotyl length.";
Plant J. 25:213-221(2001).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
[3]
GENOME REANNOTATION.
STRAIN=cv. Columbia;
PubMed=27862469; DOI=10.1111/tpj.13415;
Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
Town C.D.;
"Araport11: a complete reannotation of the Arabidopsis thaliana
reference genome.";
Plant J. 89:789-804(2017).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
PubMed=14593172; DOI=10.1126/science.1088305;
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis
genome.";
Science 302:842-846(2003).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
"Arabidopsis ORF clones.";
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
[6]
FUNCTION, CHARACTERIZATION, AND INDUCTION.
PubMed=15659623; DOI=10.1105/tpc.104.026690;
Staswick P.E., Serban B., Rowe M., Tiryaki I., Maldonado M.T.,
Maldonado M.C., Suza W.;
"Characterization of an Arabidopsis enzyme family that conjugates
amino acids to indole-3-acetic acid.";
Plant Cell 17:616-627(2005).
[7]
NOMENCLATURE.
PubMed=12036261; DOI=10.1023/A:1015207114117;
Hagen G., Guilfoyle T.J.;
"Auxin-responsive gene expression: genes, promoters and regulatory
factors.";
Plant Mol. Biol. 49:373-385(2002).
-!- FUNCTION: Catalyzes the synthesis of indole-3-acetic acid (IAA)-
amino acid conjugates, providing a mechanism for the plant to cope
with the presence of excess auxin. Strongly reactive with Glu,
Gln, Trp, Asp, Ala, Leu, Phe, Gly, Tyr, Met, Ile and Val. Little
or no product formation with His, Ser, Thr, Arg, Lys, or Cys. Also
active on pyruvic and butyric acid analogs of IAA, PAA and the
synthetic auxin naphthaleneacetic acid (NAA). The two chlorinated
synthetic auxin herbicides 2,4-D and 3,6-dichloro-o-anisic acid
(dicamba) cannot be used as substrates (PubMed:15659623). Involved
in auxin signal transduction. Inhibits shoot and hypocotyl cell
elongation, and lateral root cell differentiation in light
(PubMed:11169197). {ECO:0000269|PubMed:11169197,
ECO:0000269|PubMed:15659623}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
Vmax=244 nmol/min/mg enzyme with Asp as substrate (at pH 9.0);
pH dependence:
Optimum pH is 9.0. No activity at or below pH 7.5.;
-!- TISSUE SPECIFICITY: Expressed in cotyledons, stipules, true
leaves, hypocotyls, and all parts of the roots. Not detected in
flowers.
-!- INDUCTION: By auxin. Not regulated by light.
{ECO:0000269|PubMed:11169197, ECO:0000269|PubMed:15659623}.
-!- MISCELLANEOUS: The gain-of-function mutant dfl1-D (T-DNA tagging)
has a short hypocotyl under blue, red and far-red light, but not
in darkness, shows an exaggerated dwarf phenotype in the adult
plant caused by the inhibition of cell elongation in shoots, and
inhibition of the lateral root growth without any reduction of
primary root length. {ECO:0000269|PubMed:11169197}.
-!- SIMILARITY: Belongs to the IAA-amido conjugating enzyme family.
{ECO:0000305}.
-----------------------------------------------------------------------
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EMBL; AB050596; BAB17304.1; -; mRNA.
EMBL; AB026634; BAA97524.1; -; Genomic_DNA.
EMBL; CP002688; AED96504.1; -; Genomic_DNA.
EMBL; AY069892; AAL47444.1; -; mRNA.
EMBL; BT021108; AAX12878.1; -; mRNA.
RefSeq; NP_200262.1; NM_124831.3.
UniGene; At.9013; -.
ProteinModelPortal; Q9LSQ4; -.
SMR; Q9LSQ4; -.
BioGrid; 20783; 1.
STRING; 3702.AT5G54510.1; -.
PaxDb; Q9LSQ4; -.
PRIDE; Q9LSQ4; -.
EnsemblPlants; AT5G54510.1; AT5G54510.1; AT5G54510.
GeneID; 835539; -.
Gramene; AT5G54510.1; AT5G54510.1; AT5G54510.
KEGG; ath:AT5G54510; -.
Araport; AT5G54510; -.
TAIR; locus:2147314; AT5G54510.
eggNOG; ENOG410IHQ2; Eukaryota.
eggNOG; ENOG410XT6V; LUCA.
HOGENOM; HOG000238302; -.
InParanoid; Q9LSQ4; -.
KO; K14487; -.
OMA; CLNGNTP; -.
OrthoDB; EOG093605R6; -.
PhylomeDB; Q9LSQ4; -.
BioCyc; ARA:AT5G54510-MONOMER; -.
BioCyc; MetaCyc:AT5G54510-MONOMER; -.
Reactome; R-ATH-6798695; Neutrophil degranulation.
PRO; PR:Q9LSQ4; -.
Proteomes; UP000006548; Chromosome 5.
ExpressionAtlas; Q9LSQ4; baseline and differential.
Genevisible; Q9LSQ4; AT.
GO; GO:0009507; C:chloroplast; IDA:TAIR.
GO; GO:0005737; C:cytoplasm; ISS:TAIR.
GO; GO:0010279; F:indole-3-acetic acid amido synthetase activity; IDA:TAIR.
GO; GO:0010252; P:auxin homeostasis; TAS:TAIR.
GO; GO:0009734; P:auxin-activated signaling pathway; IMP:TAIR.
GO; GO:0009733; P:response to auxin; IMP:TAIR.
GO; GO:0009826; P:unidimensional cell growth; IMP:TAIR.
InterPro; IPR004993; GH3.
PANTHER; PTHR31901; PTHR31901; 1.
Pfam; PF03321; GH3; 1.
1: Evidence at protein level;
Complete proteome; Ligase; Reference proteome.
CHAIN 1 612 Indole-3-acetic acid-amido synthetase
GH3.6.
/FTId=PRO_0000203575.
CONFLICT 604 604 P -> H (in Ref. 4; AAL47444).
{ECO:0000305}.
SEQUENCE 612 AA; 68897 MW; CD7E498E353F7144 CRC64;
MPEAPKIAAL EVSDESLAEK NKNKLQFIED VTTNADDVQR RVLEEILSRN ADVEYLKRHG
LEGRTDRETF KHIMPVVTYE DIQPEINRIA NGDKSQVLCS NPISEFLTSS GTSGGERKLM
PTIEEELDRR SLLYSLLMPV MDQFVPGLDK GKGMYFLFIK SESKTPGGLP ARPVLTSYYK
SSHFKNRPYD PYTNYTSPNQ TILCSDSYQS MYSQMLCGLC QHKEVLRVGA VFASGFIRAI
KFLEKHWPEL ARDIRTGTLS SEITDSSVRE AVGEILKPDP KLADFVESEC RKTSWQGIIT
RLWPNTKYVD VIVTGTMSQY IPTLDYYSNG LPLVCTMYAS SECYFGVNLR PLCKPSEVSY
TLIPNMAYFE FLPVHRNSGV TSSISLPKAL TEKEQQELVD LVDVKLGQEY ELVVTTYAGL
YRYRVGDVLS VAGFKNNAPQ FSFICRKNVV LSIDSDKTDE VELQNAVKNA VTHLVPFDAS
LSEYTSYADT SSIPGHYVLF WELCLNGNTP IPPSVFEDCC LTIEESLNSV YRQGRVSDKS
IGPLEIKMVE SGTFDKLMDY AISLGASINQ YKTPRCVKFA PIIELLNSRV VDSYFSPKCP
KWSPGHKQWG SN


Related products :

Catalog number Product name Quantity
AS09 441 Antibody: IAA | indole-3-acetic acid (C1') (5mg), Immunogen: BSA-conjugated, via C1 carboxyl group, indole-3-acetic acid (C1'), Host: rabbit, polyclonal, Confirmed reactivity: IAA | indole-3-acetic ac 5 mg
AS09 420 Antibody: IAA | indole-3-acetic acid (C1') (1mg), Immunogen: BSA-conjugated, via C1 carboxyl group, indole-3-acetic acid (C1'), Host: rabbit, polyclonal, Confirmed reactivity: IAA | indole-3-acetic ac 1 mg
AS09 421 Antibody: IAA | indole-3-acetic acid (N1) (for immunolocalization), Immunogen: BSA-conjugated, via C1 carobxyl group, indole-3-acetic acid (N1), Host: rabbit, polyclonal, Confirmed reactivity: indole- 200
AS09 445 Antibody: IAA | indole-3-acetic acid (C1') (for immunolocalization), Immunogen: BSA- conjugated, via C1 carboxyl group, indole-3-acetic acid (C1'), Host: rabbit, polyclonal, Confirmed reactivity: indo 200
AS06 193 Antibody: IAA | indole 3 acetic acid, Immunogen: BSA-conjugated synthetic indole 3 acetic acid, Host: rabbit, polyclonal, Confirmed reactivity: Arabidoppsis thaliana, Euphorbia pulcherrima, Medicago s 100
AS06193 IAA | indole 3 acetic acid 100 ul
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