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Isoflavone 4'-O-methyltransferase (MtHI4'OMT) (EC 2.1.1.46) (2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase) (EC 2.1.1.212)

 I4OMT_MEDTR             Reviewed;         364 AA.
Q29U70;
27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
04-APR-2006, sequence version 1.
20-JUN-2018, entry version 61.
RecName: Full=Isoflavone 4'-O-methyltransferase;
Short=MtHI4'OMT;
EC=2.1.1.46 {ECO:0000269|PubMed:17172354};
AltName: Full=2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase;
EC=2.1.1.212 {ECO:0000269|PubMed:17172354};
Name=HI4'OMT;
Medicago truncatula (Barrel medic) (Medicago tribuloides).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae;
Trifolieae; Medicago.
NCBI_TaxID=3880;
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY,
BIOPHYSICOCHEMICAL PROPERTIES, X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS)
OF 7-364 IN COMPLEX WITH SUBSTRATES, AND SUBUNIT.
PubMed=17172354; DOI=10.1105/tpc.106.041376;
Liu C.J., Deavours B.E., Richard S.B., Ferrer J.L., Blount J.W.,
Huhman D., Dixon R.A., Noel J.P.;
"Structural basis for dual functionality of isoflavonoid O-
methyltransferases in the evolution of plant defense responses.";
Plant Cell 18:3656-3669(2006).
-!- FUNCTION: 2-hydroxyisoflavanone 4'-O-methyltransferase involved in
the biosynthesis of the phytoalexin medicarpin. Has also an in
vitro (+)-6a-hydroxymaackiain-3-0-methyltransferase activity,
converting the pterocarpan 6a-hydroxymaackiain into pisatin. No
activity with di- or trihydroxylated isoflavones, including
daidzein and genistein, or with (-)-medicarpin and maackiain. The
dual activity for either 3- or 4'-O-methylation depends upon
substrate availability. {ECO:0000269|PubMed:17172354}.
-!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + a 4'-
hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-
methoxyisoflavone. {ECO:0000269|PubMed:17172354}.
-!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 2,4',7-
trihydroxyisoflavanone = S-adenosyl-L-homocysteine + 2,7-
dihydroxy-4'-methoxyisoflavanone. {ECO:0000269|PubMed:17172354}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=73.3 uM for 2,7,4'-trihydroxyisoflavanone
{ECO:0000269|PubMed:17172354};
KM=62.1 uM for 6a-hydroxymaackiain
{ECO:0000269|PubMed:17172354};
KM=99.8 uM for S-adenosyl-L-methionine
{ECO:0000269|PubMed:17172354};
Vmax=4.0 nmol/min/mg enzyme with 2,7,4'-trihydroxyisoflavanone
as substrate {ECO:0000269|PubMed:17172354};
Vmax=17.9 nmol/min/mg enzyme with 6a-hydroxymaackiain as
substrate {ECO:0000269|PubMed:17172354};
Vmax=23.9 nmol/min/mg enzyme toward S-adenosyl-L-methionine for
the 4'-O-methyltransferase activity
{ECO:0000269|PubMed:17172354};
-!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:17172354}.
-!- SIMILARITY: Belongs to the class I-like SAM-binding
methyltransferase superfamily. Cation-independent O-
methyltransferase family. COMT subfamily. {ECO:0000255|PROSITE-
ProRule:PRU01020}.
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EMBL; AY942158; AAY18581.1; -; mRNA.
RefSeq; XP_013456930.1; XM_013601476.1.
UniGene; Mtr.15361; -.
PDB; 1ZG3; X-ray; 2.35 A; A=7-364.
PDB; 1ZGA; X-ray; 2.35 A; A=8-364.
PDB; 1ZGJ; X-ray; 2.50 A; A=11-364.
PDB; 1ZHF; X-ray; 2.50 A; A=8-364.
PDBsum; 1ZG3; -.
PDBsum; 1ZGA; -.
PDBsum; 1ZGJ; -.
PDBsum; 1ZHF; -.
ProteinModelPortal; Q29U70; -.
SMR; Q29U70; -.
ProMEX; Q29U70; -.
EnsemblPlants; KEH30961; KEH30961; MTR_4g088190.
GeneID; 25493072; -.
Gramene; KEH30961; KEH30961; MTR_4g088190.
KEGG; ag:AAY18581; -.
KEGG; mtr:MTR_4g088190; -.
KO; K13259; -.
BRENDA; 2.1.1.212; 3201.
BRENDA; 2.1.1.270; 3201.
EvolutionaryTrace; Q29U70; -.
ExpressionAtlas; Q29U70; differential.
GO; GO:0102670; F:2,7,4'-trihydroxyisoflavanone-4'-O-methyltransferase activity; IEA:UniProtKB-EC.
GO; GO:0030746; F:isoflavone 4'-O-methyltransferase activity; IEA:UniProtKB-EC.
GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
Gene3D; 1.10.10.10; -; 1.
InterPro; IPR016461; O-MeTrfase_COMT.
InterPro; IPR001077; O_MeTrfase_2.
InterPro; IPR012967; Plant_MeTrfase_dimerisation.
InterPro; IPR029063; SAM-dependent_MTases.
InterPro; IPR036388; WH-like_DNA-bd_sf.
InterPro; IPR036390; WH_DNA-bd_sf.
Pfam; PF08100; Dimerisation; 1.
Pfam; PF00891; Methyltransf_2; 1.
PIRSF; PIRSF005739; O-mtase; 1.
SUPFAM; SSF46785; SSF46785; 1.
SUPFAM; SSF53335; SSF53335; 1.
PROSITE; PS51683; SAM_OMT_II; 1.
1: Evidence at protein level;
3D-structure; Methyltransferase; S-adenosyl-L-methionine; Transferase.
CHAIN 1 364 Isoflavone 4'-O-methyltransferase.
/FTId=PRO_0000411977.
REGION 206 209 S-adenosyl-L-methionine binding.
REGION 230 231 S-adenosyl-L-methionine binding.
REGION 250 251 S-adenosyl-L-methionine binding.
COMPBIAS 96 99 Poly-Glu.
ACT_SITE 268 268 Proton acceptor. {ECO:0000255|PROSITE-
ProRule:PRU01020}.
BINDING 230 230 S-adenosyl-L-methionine.
{ECO:0000255|PROSITE-ProRule:PRU01020}.
BINDING 264 264 S-adenosyl-L-methionine.
TURN 8 10 {ECO:0000244|PDB:1ZG3}.
HELIX 16 24 {ECO:0000244|PDB:1ZG3}.
HELIX 27 39 {ECO:0000244|PDB:1ZG3}.
HELIX 41 48 {ECO:0000244|PDB:1ZG3}.
HELIX 54 60 {ECO:0000244|PDB:1ZG3}.
TURN 65 67 {ECO:0000244|PDB:1ZG3}.
HELIX 68 80 {ECO:0000244|PDB:1ZG3}.
STRAND 83 89 {ECO:0000244|PDB:1ZG3}.
STRAND 92 95 {ECO:0000244|PDB:1ZG3}.
STRAND 99 104 {ECO:0000244|PDB:1ZG3}.
HELIX 106 109 {ECO:0000244|PDB:1ZG3}.
STRAND 113 115 {ECO:0000244|PDB:1ZGA}.
HELIX 120 126 {ECO:0000244|PDB:1ZG3}.
HELIX 129 132 {ECO:0000244|PDB:1ZG3}.
HELIX 133 137 {ECO:0000244|PDB:1ZG3}.
HELIX 138 143 {ECO:0000244|PDB:1ZG3}.
HELIX 150 155 {ECO:0000244|PDB:1ZG3}.
HELIX 159 163 {ECO:0000244|PDB:1ZG3}.
HELIX 166 168 {ECO:0000244|PDB:1ZG3}.
HELIX 169 184 {ECO:0000244|PDB:1ZG3}.
HELIX 186 192 {ECO:0000244|PDB:1ZG3}.
HELIX 194 198 {ECO:0000244|PDB:1ZG3}.
STRAND 201 206 {ECO:0000244|PDB:1ZG3}.
HELIX 212 220 {ECO:0000244|PDB:1ZG3}.
STRAND 224 230 {ECO:0000244|PDB:1ZG3}.
HELIX 232 235 {ECO:0000244|PDB:1ZG3}.
STRAND 242 248 {ECO:0000244|PDB:1ZG3}.
TURN 251 253 {ECO:0000244|PDB:1ZG3}.
STRAND 259 265 {ECO:0000244|PDB:1ZG3}.
HELIX 267 269 {ECO:0000244|PDB:1ZG3}.
HELIX 272 285 {ECO:0000244|PDB:1ZG3}.
HELIX 286 292 {ECO:0000244|PDB:1ZG3}.
STRAND 294 299 {ECO:0000244|PDB:1ZG3}.
HELIX 309 326 {ECO:0000244|PDB:1ZG3}.
HELIX 333 342 {ECO:0000244|PDB:1ZG3}.
STRAND 347 353 {ECO:0000244|PDB:1ZG3}.
TURN 354 356 {ECO:0000244|PDB:1ZG3}.
STRAND 357 363 {ECO:0000244|PDB:1ZG3}.
SEQUENCE 364 AA; 40754 MW; C7C83C858A4BC9E5 CRC64;
MAFSTNGSEE SELYHAQIHL YKHVYNFVSS MALKSAMELG IADAIHNHGK PMTLSELASS
LKLHPSKVNI LHRFLRLLTH NGFFAKTIVK GKEGDEEEEI AYSLTPPSKL LISGKPTCLS
SIVKGALHPS SLDMWSSSKK WFNEDKEQTL FECATGESFW DFLNKDSESS TLSMFQDAMA
SDSRMFKLVL QENKRVFEGL ESLVDVGGGT GGVTKLIHEI FPHLKCTVFD QPQVVGNLTG
NENLNFVGGD MFKSIPSADA VLLKWVLHDW NDEQSLKILK NSKEAISHKG KDGKVIIIDI
SIDETSDDRG LTELQLDYDL VMLTMFLGKE RTKQEWEKLI YDAGFSSYKI TPISGFKSLI
EVYP


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