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Ketol-acid reductoisomerase (NADP( )) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase)
A0A126QXH1_9EURY Unreviewed; 327 AA.
A0A126QXH1;
11-MAY-2016, integrated into UniProtKB/TrEMBL.
11-MAY-2016, sequence version 1.
28-MAR-2018, entry version 16.
RecName: Full=Ketol-acid reductoisomerase (NADP(+)) {ECO:0000256|HAMAP-Rule:MF_00435};
Short=KARI {ECO:0000256|HAMAP-Rule:MF_00435};
EC=1.1.1.86 {ECO:0000256|HAMAP-Rule:MF_00435};
AltName: Full=Acetohydroxy-acid isomeroreductase {ECO:0000256|HAMAP-Rule:MF_00435};
Short=AHIR {ECO:0000256|HAMAP-Rule:MF_00435};
AltName: Full=Alpha-keto-beta-hydroxylacyl reductoisomerase {ECO:0000256|HAMAP-Rule:MF_00435};
Name=ilvC {ECO:0000256|HAMAP-Rule:MF_00435};
ORFNames=AUP07_1312 {ECO:0000313|EMBL:AMK14349.1};
methanogenic archaeon mixed culture ISO4-G1.
Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
environmental samples.
NCBI_TaxID=1452364 {ECO:0000313|EMBL:AMK14349.1};
[1] {ECO:0000313|EMBL:AMK14349.1}
NUCLEOTIDE SEQUENCE.
STRAIN=ISO4-G1 {ECO:0000313|EMBL:AMK14349.1};
PubMed=27056226;
Kelly W.J., Li D., Lambie S.C., Jeyanathan J., Cox F., Li Y.,
Attwood G.T., Altermann E., Leahy S.C.;
"Complete Genome Sequence of Methanogenic Archaeon ISO4-G1, a Member
of the Methanomassiliicoccales, Isolated from a Sheep Rumen.";
Genome Announc. 4:0-0(2016).
-!- FUNCTION: Involved in the biosynthesis of branched-chain amino
acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-
acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-
dihydroxy-isovalerate. In the isomerase reaction, S2AL is
rearranged via a Mg-dependent methyl migration to produce 3-
hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction,
this 2-ketoacid undergoes a metal-dependent reduction by NADPH to
yield (R)-2,3-dihydroxy-isovalerate. {ECO:0000256|HAMAP-
Rule:MF_00435}.
-!- CATALYTIC ACTIVITY: (2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+)
= (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
{ECO:0000256|HAMAP-Rule:MF_00435}.
-!- CATALYTIC ACTIVITY: (2R,3R)-2,3-dihydroxy-3-methylpentanoate +
NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
{ECO:0000256|HAMAP-Rule:MF_00435}.
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Evidence={ECO:0000256|HAMAP-Rule:MF_00435};
Note=Binds 2 magnesium ions per subunit. {ECO:0000256|HAMAP-
Rule:MF_00435};
-!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-
isoleucine from 2-oxobutanoate: step 2/4. {ECO:0000256|HAMAP-
Rule:MF_00435}.
-!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine
from pyruvate: step 2/4. {ECO:0000256|HAMAP-Rule:MF_00435}.
-!- SIMILARITY: Belongs to the ketol-acid reductoisomerase family.
{ECO:0000256|HAMAP-Rule:MF_00435}.
-!- CAUTION: Lacks conserved residue(s) required for the propagation
of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00435}.
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EMBL; CP013703; AMK14349.1; -; Genomic_DNA.
PATRIC; fig|1452364.3.peg.1344; -.
UniPathway; UPA00047; UER00056.
UniPathway; UPA00049; UER00060.
GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
GO; GO:0004455; F:ketol-acid reductoisomerase activity; IEA:UniProtKB-UniRule.
GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
GO; GO:0050661; F:NADP binding; IEA:InterPro.
GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniRule.
GO; GO:0009099; P:valine biosynthetic process; IEA:UniProtKB-UniRule.
HAMAP; MF_00435; IlvC; 1.
InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
InterPro; IPR000506; AcH_isomrdctse_C.
InterPro; IPR013116; IlvN.
InterPro; IPR013023; KARI.
InterPro; IPR014359; KARI_prok.
InterPro; IPR036291; NAD(P)-bd_dom_sf.
PANTHER; PTHR21371; PTHR21371; 1.
Pfam; PF01450; IlvC; 1.
Pfam; PF07991; IlvN; 1.
PIRSF; PIRSF000116; IlvC_gammaproteo; 1.
SUPFAM; SSF48179; SSF48179; 1.
SUPFAM; SSF51735; SSF51735; 1.
TIGRFAMs; TIGR00465; ilvC; 1.
3: Inferred from homology;
Amino-acid biosynthesis {ECO:0000256|HAMAP-Rule:MF_00435};
Branched-chain amino acid biosynthesis {ECO:0000256|HAMAP-
Rule:MF_00435}; Coiled coil {ECO:0000256|SAM:Coils};
Isomerase {ECO:0000313|EMBL:AMK14349.1};
Magnesium {ECO:0000256|HAMAP-Rule:MF_00435};
Metal-binding {ECO:0000256|HAMAP-Rule:MF_00435};
NADP {ECO:0000256|HAMAP-Rule:MF_00435};
Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_00435}.
DOMAIN 14 177 IlvN. {ECO:0000259|Pfam:PF07991}.
DOMAIN 183 324 IlvC. {ECO:0000259|Pfam:PF01450}.
NP_BIND 24 27 NADP. {ECO:0000256|HAMAP-Rule:MF_00435}.
COILED 301 321 {ECO:0000256|SAM:Coils}.
ACT_SITE 107 107 {ECO:0000256|HAMAP-Rule:MF_00435}.
METAL 190 190 Magnesium 1. {ECO:0000256|HAMAP-
Rule:MF_00435}.
METAL 190 190 Magnesium 2. {ECO:0000256|HAMAP-
Rule:MF_00435}.
METAL 194 194 Magnesium 1. {ECO:0000256|HAMAP-
Rule:MF_00435}.
METAL 226 226 Magnesium 2. {ECO:0000256|HAMAP-
Rule:MF_00435}.
METAL 230 230 Magnesium 2. {ECO:0000256|HAMAP-
Rule:MF_00435}.
BINDING 47 47 NADP. {ECO:0000256|HAMAP-Rule:MF_00435}.
BINDING 52 52 NADP. {ECO:0000256|HAMAP-Rule:MF_00435}.
BINDING 133 133 NADP; via amide nitrogen.
{ECO:0000256|HAMAP-Rule:MF_00435}.
BINDING 251 251 Substrate. {ECO:0000256|HAMAP-
Rule:MF_00435}.
SEQUENCE 327 AA; 35940 MW; D1AE6C108139ABF3 CRC64;
MQLYHDGDVD LSIFEGKKVA VLGYGSQGRA QALCFHDSGM DVTVGVRRNG PSWNQAKEDG
LKVAELADAV KGADVVVVLL PDEVQASVYK ESVGPNLKSG AALAFAHGFA ITFKTIVPPK
DVDVIMMAPK GPGNMVRQIF LEGFGVPAMV CINQDHTGNA MKIALGLAKG IGATRSGVFE
STFERETHTD LFGEQAVLCG GQTGLIKNGF ETLVEAGYPP EMAYFEVLHE MKQVTELINR
GGLDLMWHEV SNTAEFGGLT RRDRVVNESS KEEMRKILKE IQDGTFKNEW VSEFDNGLVN
LKRMEDEEAK LQMEVVGKEI RRLFQRK
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Pathways :
WP105: Fatty Acid Beta Oxidation 2
WP1061: Fatty Acid Beta Oxidation
WP1107: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP1177: Fatty Acid Beta Oxidation
WP1207: Fatty Acid Beta Oxidation
WP1226: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP1237: Fatty Acid Beta Oxidation
WP126: Fatty Acid Beta Oxidation 1
WP1269: Fatty Acid Beta Oxidation
WP1307: Fatty Acid Beta Oxidation
WP143: Fatty Acid Beta Oxidation
WP148: Fatty Acid Beta Oxidation 2
WP1618: alpha-Linolenic acid metabolism
WP169: Fatty Acid Beta Oxidation 3
WP187: Fatty Acid Beta Oxidation 2
WP209: Fatty Acid Beta Oxidation Meta
WP25: Fatty Acid Beta Oxidation 3
WP368: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP39: Fatty Acid Beta Oxidation 1
WP401: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP406: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP419: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP448: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP498: Mitochondrial LC-Fatty Acid Beta-Oxidation
WP499: Fatty Acid Beta Oxidation 3
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[ilvC Igag_1561] Ketol-acid reductoisomerase (NAD(P)(+)) (KARI) (EC 1.1.1.383) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC Rv3001c MTV012.15c] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC HMPREF0762_00954] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC Aaci_2227] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC GZ26G2_30] Ketol-acid reductoisomerase (NAD(+)) (KARI) (EC 1.1.1.382) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC sll1363] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC LL1226 L0080] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC STM3909 STMD1.81] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 2) (Ketol-acid reductoisomerase type II)
[At3g58610 F14P22.200] Ketol-acid reductoisomerase, chloroplastic (EC 1.1.1.86) (Acetohydroxy-acid reductoisomerase) (Alpha-keto-beta-hydroxylacyl reductoisomerase)
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[ILV5 YLR355C L9638.7] Ketol-acid reductoisomerase, mitochondrial (EC 1.1.1.86) (Acetohydroxy-acid reductoisomerase) (Alpha-keto-beta-hydroxylacyl reductoisomerase)
[ilvC PA4694] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[Os05g0573700 LOC_Os05g49800 OJ1735_C10.18] Ketol-acid reductoisomerase, chloroplastic (EC 1.1.1.86) (Acetohydroxy-acid reductoisomerase) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Protein KARI)
[ilvC Avin_42500] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC HY04AAS1_0065] Ketol-acid reductoisomerase (NAD(P)(+)) (KARI) (EC 1.1.1.383) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC AF_1985] Ketol-acid reductoisomerase (NAD(P)(+)) (KARI) (EC 1.1.1.383) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC Swol_2144] Ketol-acid reductoisomerase (NAD(P)(+)) (KARI) (EC 1.1.1.383) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC Msed_1927] Ketol-acid reductoisomerase (NAD(P)(+)) (KARI) (EC 1.1.1.383) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC Tph_c19850] Ketol-acid reductoisomerase (NAD(+)) (KARI) (EC 1.1.1.382) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC c4696] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 2) (Ketol-acid reductoisomerase type II)
[ilvC Cgl1273 cg1437] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC ilvC-1 ilvC1 SSO0576] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC ML1694 MLCB637.22] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC DR_1519] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC MJ1543] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC Z5285 ECs4708] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 2) (Ketol-acid reductoisomerase type II)
[ilvC HI_0682] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 2) (Ketol-acid reductoisomerase type II)
[ilvC MTH_1442] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 1) (Ketol-acid reductoisomerase type I)
[ilvC STY3648 t3389] Ketol-acid reductoisomerase (NADP(+)) (KARI) (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (AHIR) (Alpha-keto-beta-hydroxylacyl reductoisomerase) (Ketol-acid reductoisomerase type 2) (Ketol-acid reductoisomerase type II)
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