Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Large T antigen (LT) (LT-AG) (EC 3.6.4.-)

 LT_POVSM                Reviewed;         655 AA.
A8Y984;
16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
15-JAN-2008, sequence version 1.
25-OCT-2017, entry version 49.
RecName: Full=Large T antigen;
Short=LT;
Short=LT-AG;
EC=3.6.4.-;
Name=large T;
Squirrel monkey polyomavirus.
Viruses; dsDNA viruses, no RNA stage; Polyomaviridae.
NCBI_TaxID=452475;
NCBI_TaxID=39432; Saimiri boliviensis boliviensis (Bolivian squirrel monkey).
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Squi0106;
PubMed=18089736; DOI=10.1099/vir.0.83287-0;
Verschoor E.J., Groenewoud M.J., Fagrouch Z., Kewalapat A.,
van Gessel S., Kik M.J., Heeney J.L.;
"Molecular characterization of the first polyomavirus from a New World
primate: squirrel monkey polyomavirus.";
J. Gen. Virol. 89:130-137(2008).
-!- FUNCTION: Isoform large T antigen is a key early protein essential
for both driving viral replication and inducing cellular
transformation. Plays a role in viral genome replication by
driving entry of quiescent cells into the cell cycle and by
autoregulating the synthesis of viral early mRNA. Displays highly
oncogenic activities by corrupting the host cellular checkpoint
mechanisms that guard cell division and the transcription,
replication, and repair of DNA. Participates in the modulation of
cellular gene expression preceeding viral DNA replication. This
step involves binding to host key cell cycle regulators
retinoblastoma protein RB1/pRb and TP53. Induces the disassembly
of host E2F1 transcription factors from RB1, thus promoting
transcriptional activation of E2F1-regulated S-phase genes.
Inhibits host TP53 binding to DNA, abrogating the ability of TP53
to stimulate gene expression. Plays the role of a TFIID-associated
factor (TAF) in transcription initiation for all three RNA
polymerases, by stabilizing the TBP-TFIIA complex on promoters.
Initiates viral DNA replication and unwinding via interactions
with the viral origin of replication. Binds two adjacent sites in
the SV40 origin. The replication fork movement is facilitated by
Large T antigen helicase activity. Activates the transcription of
viral late mRNA, through host TBP and TFIIA stabilization.
Interferes with histone deacetylation mediated by HDAC1, leading
to activation of transcription (By similarity). {ECO:0000250}.
-!- SUBUNIT: Forms homohexamers in the presence of ATP. Interacts with
host HDAC1. Interacts (via LXCXE domain) with host RB1; the
interaction induces the aberrant dissociation of RB1-E2F1 complex
thereby disrupting RB1's activity. Interacts (via LXCXE domain)
with host pRB-related proteins RBL1 and RBL2. Interacts (via C-
terminus) with host TOP1 and POLA1 allowing DNA replication.
Interacts with host TP53, inhibiting TP53 binding to DNA.
Interacts with host preinitiation complex components TBP, TFIIA
and TFIID to regulate transcription initiation (By similarity).
{ECO:0000250}.
-!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250}.
-!- ALTERNATIVE PRODUCTS:
Event=Alternative splicing; Named isoforms=2;
Name=Large T antigen;
IsoId=A8Y984-1; Sequence=Displayed;
Name=Small t antigen;
IsoId=A8Y985-1; Sequence=External;
-!- DOMAIN: The J domain is essential for multiple viral activities,
including virion assembly, viral DNA replication, transformation
and transcriptional activation. {ECO:0000250}.
-!- DOMAIN: The LXCXE motif specifically binds to host pRB, RBL1, and
RBL2. {ECO:0000250}.
-!- DOMAIN: The zinc finger region contributes to protein-protein
interactions essential for the assembly of stable T-antigen
hexamers at the origin of replication. The hexamers are required
for subsequent alterations in the structure of origin DNA (By
similarity). {ECO:0000250}.
-!- DOMAIN: The ATP binding/ATPase domain is required for proper
hexamer assembly and helicase activity. {ECO:0000250}.
-!- PTM: Phosphorylated on both serine and threonine residues. Small t
antigen inhibits the dephosphorylation by the AC form of PP2A (By
similarity). {ECO:0000250}.
-!- PTM: O-Glycosylated near the C-terminal region. {ECO:0000250}.
-!- PTM: Acetylated by CBP in a TP53-dependent manner.
{ECO:0000250|UniProtKB:P03070}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; AM748741; CAO03083.2; -; Genomic_DNA.
RefSeq; YP_001531349.1; NC_009951.1. [A8Y984-1]
SMR; A8Y984; -.
PRIDE; A8Y984; -.
GeneID; 5714861; -.
KEGG; vg:5714861; -.
Proteomes; UP000135044; Genome.
GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0003688; F:DNA replication origin binding; IEA:InterPro.
GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
GO; GO:0039645; P:modulation by virus of host G1/S transition checkpoint; IEA:UniProtKB-KW.
GO; GO:0039576; P:suppression by virus of host JAK1 activity; IEA:UniProtKB-KW.
GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
Gene3D; 1.10.10.510; -; 1.
Gene3D; 1.10.287.110; -; 1.
InterPro; IPR036869; DnaJ_dom_sf.
InterPro; IPR001623; DnaJ_domain.
InterPro; IPR014015; Helicase_SF3_DNA-vir.
InterPro; IPR016392; Lg_T_Ag_polyomavir.
InterPro; IPR010932; Lg_T_Ag_Polyomavir_C.
InterPro; IPR027417; P-loop_NTPase.
InterPro; IPR003133; T_Ag_DNA-bd.
InterPro; IPR017910; Znf_lg_T-Ag_D1-typ.
InterPro; IPR037102; Znf_lg_T-Ag_D1_dom_sf.
Pfam; PF06431; Polyoma_lg_T_C; 1.
Pfam; PF02217; T_Ag_DNA_bind; 1.
PIRSF; PIRSF003368; Large_T_antigen_polyomaV; 1.
SMART; SM00271; DnaJ; 1.
SUPFAM; SSF46565; SSF46565; 1.
SUPFAM; SSF52540; SSF52540; 1.
PROSITE; PS51206; SF3_HELICASE_1; 1.
PROSITE; PS51287; T_AG_OBD; 1.
PROSITE; PS51341; ZF_LTAG_D1; 1.
3: Inferred from homology;
Acetylation; Alternative splicing; ATP-binding; Complete proteome;
DNA replication; DNA-binding; Early protein;
G1/S host cell cycle checkpoint dysregulation by virus; Host nucleus;
Host-virus interaction; Hydrolase;
Inhibition of host innate immune response by virus;
Inhibition of host interferon signaling pathway by virus;
Inhibition of host JAK1 by virus; Metal-binding;
Modulation of host cell cycle by virus; Nucleotide-binding; Oncogene;
Phosphoprotein; Reference proteome; Viral immunoevasion; Zinc;
Zinc-finger.
CHAIN 1 655 Large T antigen.
/FTId=PRO_0000356264.
DOMAIN 12 84 J.
DOMAIN 411 570 SF3 helicase. {ECO:0000255|PROSITE-
ProRule:PRU00551}.
DNA_BIND 149 264 T-ag OBD. {ECO:0000255|PROSITE-
ProRule:PRU00620}.
ZN_FING 274 366 T-ag D1-type. {ECO:0000255|PROSITE-
ProRule:PRU00671}.
NP_BIND 437 444 ATP. {ECO:0000255|PROSITE-
ProRule:PRU00551}.
MOTIF 101 105 LXCXE motif.
MOTIF 135 142 Nuclear localization signal.
COMPBIAS 137 140 Poly-Lys.
COMPBIAS 583 589 Poly-Leu.
MOD_RES 1 1 N-acetylmethionine; by host.
{ECO:0000250|UniProtKB:P03070}.
MOD_RES 110 110 Phosphoserine; by host.
{ECO:0000250|UniProtKB:P03070}.
MOD_RES 134 134 Phosphothreonine; by host.
{ECO:0000250|UniProtKB:P03070}.
SEQUENCE 655 AA; 75109 MW; CD1FDC09A8F561FE CRC64;
MDYTLTREES KLLMELLGLP MEQYGNFPLM RKAFLQKCKI MHPDKGGDEQ TAKMLISLYK
KLESEVKSLN TDDGFSTEEI PTYGSAEWEQ WWQTFNEDFD LFCHETFTVS DDEEEGGEKR
KHSDEEEPSC SQATPPKKKK TSSAPKDMPE LLRNFLSNAI LSNKTLTCFL VYTTLEKSSL
LYSKLSEKFK PTFISRHKLD NEGLIFLITP SKHRVSAITN FCSNLCSVSF LIVKAVIKEY
SCYCALCVEP FVLVVENIPG GLNSDFFDAP QEASKNVSWK LIGEYALSIM CDDLFLLLGL
YKEFAVNPST CSKCDQKVIV DHYKYHSLHY ANAMLFTDCK NQKAICQQAV DGVLAFRRVQ
TAQLTRKQLL AKRFEYHFNK LEQVFSAKSE VCIETYMAGV CWFECLLPEV NMKNFILQYL
ECVVQNVPKK RFWCFTGPVN TGKTTLAAAL LDLCGGKSLN VNMPFDKLNF ELGVAIDQFT
VVFEDVKGQS ENKNLPTGQG ISNLDNLRDY LDGAVKVNLE KKHLNKKTQI FPPGIVTANE
YIFPLTLKVR FCKIIKFIYQ QHLFKSLKKT ELLTKHRVLQ SGLTLLLLLV FHCDVEDFLS
ELHPLVTKWR ENINHEVSWS RYLEMKENVM NGLNILEKQQ DSGIFTQTQD TECQQ


Related products :

Catalog number Product name Quantity
SCH-MCA5688 MOUSE ANTI SV40 LARGE T ANTIGEN , Product Type Monoclonal Antibody, Specificity SV40 LARGE T ANTIGEN, Target Species Viral, Host Mouse, Format Ascites, Isotypes IgG1, Applications IP, Clone 7 0.1 ml
MCA5688 MOUSE ANTI SV40 LARGE T ANTIGEN , Product Type Monoclonal Antibody, Specificity SV40 LARGE T ANTIGEN, Target Species Viral, Host Mouse, Format Ascites, Isotypes IgG1, Applications IP, Clone 7 0.1 ml
PR-841 SV40 T Antigen Simian Virus 40 _ Large Antigen human, recombinant, Sf9 insect cells 5µg
PR-841 SV40 T Antigen Simian Virus 40 _ Large Antigen human, recombinant, Sf9 insect cells 5 µg
U2005h CLIA 4F2 cell-surface antigen heavy chain,4F2 heavy chain antigen,4F2hc,Homo sapiens,Human,Lymphocyte activation antigen 4F2 large subunit,MDU1,SLC3A2 96T
U2005h CLIA kit 4F2 cell-surface antigen heavy chain,4F2 heavy chain antigen,4F2hc,Homo sapiens,Human,Lymphocyte activation antigen 4F2 large subunit,MDU1,SLC3A2 96T
E2005h ELISA 4F2 cell-surface antigen heavy chain,4F2 heavy chain antigen,4F2hc,Homo sapiens,Human,Lymphocyte activation antigen 4F2 large subunit,MDU1,SLC3A2 96T
E2005h ELISA kit 4F2 cell-surface antigen heavy chain,4F2 heavy chain antigen,4F2hc,Homo sapiens,Human,Lymphocyte activation antigen 4F2 large subunit,MDU1,SLC3A2 96T
20-272-191913 CD98 - Mouse monoclonal [JS-5] to CD98; 4F2hc; Lymphocyte activation antigen 4F2 large subunit; 4F2 heavy chain antigen; CD98 antigen Monoclonal 50 TESTS
20-272-192401 CD98 - Mouse monoclonal [MEM - 108] to CD98; 4F2hc; Lymphocyte activation antigen 4F2 large subunit; 4F2 heavy chain antigen; CD98 antigen Monoclonal 0.1 mg
orb71028 Simian Virus 40 Large Tumor Antigen Amino-Terminal peptide This is Simian Virus 40 Large Tumor Antigen Amino-Terminal peptide. For research use only. 1 mg
'H00009215-P01 LARGE antigen 2
AM00599PU-N SV40 Large T antigen 0.1 mg
RP20414 SV40 large T antigen NLS 1mg
BM1317 SV40 Large T antigen 0.1 ml
AM00599PU-N SV40 Large T antigen 0.1 mg
RP20414 SV40 large T antigen NLS 1,0mg
GTX16848 SV40 Large T antigen 1 ml
BM1317 SV40 Large T antigen 0.1 ml
GTX16879 SV40 Large T antigen 1 ml
BM1317 SV40 Large T antigen 0.1 ml
73682 IgG,SV40 LARGE T ANTIGEN 0.1 ml
BMMS-1833-B0 SV40 Large T Antigen 0.1mL
'H00005558-P01-10 DNA primase large subunit (p58) antigen 10
NB100-64651 SV40 Large T antigen 0.1 ml


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur