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Lipase-like PAD4 (EC 2.3.1.-) (Protein ENHANCED DISEASE SUSCEPTIBILITY 9) (Protein PHYTOALEXIN DEFICIENT 4) (AtPAD4)

 PAD4_ARATH              Reviewed;         541 AA.
Q9S745; B0ZUC0;
09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
01-MAY-2000, sequence version 1.
23-MAY-2018, entry version 119.
RecName: Full=Lipase-like PAD4;
EC=2.3.1.-;
AltName: Full=Protein ENHANCED DISEASE SUSCEPTIBILITY 9;
AltName: Full=Protein PHYTOALEXIN DEFICIENT 4;
Short=AtPAD4;
Name=PAD4; Synonyms=EDS9; OrderedLocusNames=At3g52430;
ORFNames=F22O6.190;
Arabidopsis thaliana (Mouse-ear cress).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
Arabidopsis.
NCBI_TaxID=3702;
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE,
IDENTIFICATION, AND INDUCTION BY P.SYRINGAE AND SALICYLIC ACID (SA).
STRAIN=cv. Columbia;
PubMed=10557364; DOI=10.1073/pnas.96.23.13583;
Jirage D., Tootle T.L., Reuber T.L., Frost L.N., Feys B.J.,
Parker J.E., Ausubel F.M., Glazebrook J.;
"Arabidopsis thaliana PAD4 encodes a lipase-like gene that is
important for salicylic acid signaling.";
Proc. Natl. Acad. Sci. U.S.A. 96:13583-13588(1999).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND REVIEW ON PLANT DEFENSE.
STRAIN=cv. Aa-0, cv. Ak-1, cv. Bay-0, cv. Columbia, cv. Di-0,
cv. Gu-0, cv. HOG, cv. Landsberg erecta, cv. Sha, cv. Sorbo, and
cv. Tsu-0;
PubMed=19064707; DOI=10.1534/genetics.108.097279;
Caldwell K.S., Michelmore R.W.;
"Arabidopsis thaliana genes encoding defense signaling and recognition
proteins exhibit contrasting evolutionary dynamics.";
Genetics 181:671-684(2009).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
PubMed=11130713; DOI=10.1038/35048706;
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M.,
Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B.,
Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P.,
De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P.,
Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F.,
Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V.,
Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S.,
Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G.,
Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B.,
Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G.,
Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J.,
Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D.,
Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
Monfort A., Argiriou A., Flores M., Liguori R., Vitale D.,
Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W.,
Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J.,
Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P.,
Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S.,
Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V.,
Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C.,
Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E.,
Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y.,
Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A.,
Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
Watanabe A., Yamada M., Yasuda M., Tabata S.;
"Sequence and analysis of chromosome 3 of the plant Arabidopsis
thaliana.";
Nature 408:820-822(2000).
[4]
GENOME REANNOTATION.
STRAIN=cv. Columbia;
PubMed=27862469; DOI=10.1111/tpj.13415;
Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
Town C.D.;
"Araport11: a complete reannotation of the Arabidopsis thaliana
reference genome.";
Plant J. 89:789-804(2017).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 227-434, AND REVIEW ON PLANT
DEFENSE.
PubMed=18245336; DOI=10.1534/genetics.107.083279;
Bakker E.G., Traw M.B., Toomajian C., Kreitman M., Bergelson J.;
"Low levels of polymorphism in genes that control the activation of
defense response in Arabidopsis thaliana.";
Genetics 178:2031-2043(2008).
[6]
FUNCTION, AND DISRUPTION PHENOTYPE.
STRAIN=cv. Columbia;
PubMed=8725243;
Glazebrook J., Rogers E.E., Ausubel F.M.;
"Isolation of Arabidopsis mutants with enhanced disease susceptibility
by direct screening.";
Genetics 143:973-982(1996).
[7]
FUNCTION, AND DISRUPTION PHENOTYPE.
STRAIN=cv. Columbia;
PubMed=9136026;
Glazebrook J., Zook M., Mert F., Kagan I., Rogers E.E., Crute I.R.,
Holub E.B., Hammerschmidt R., Ausubel F.M.;
"Phytoalexin-deficient mutants of Arabidopsis reveal that PAD4 encodes
a regulatory factor and that four PAD genes contribute to downy mildew
resistance.";
Genetics 146:381-392(1997).
[8]
FUNCTION, AND DISRUPTION PHENOTYPE.
STRAIN=cv. Columbia;
PubMed=9634589; DOI=10.1105/tpc.10.6.1021;
Zhou N., Tootle T.L., Tsui F., Klessig D.F., Glazebrook J.;
"PAD4 functions upstream from salicylic acid to control defense
responses in Arabidopsis.";
Plant Cell 10:1021-1030(1998).
[9]
FUNCTION, AND DISRUPTION PHENOTYPE.
STRAIN=cv. Columbia, and cv. Landsberg erecta;
PubMed=9881167; DOI=10.1046/j.1365-313x.1998.00319.x;
Reuber T.L., Plotnikova J.M., Dewdney J., Rogers E.E., Wood W.,
Ausubel F.M.;
"Correlation of defense gene induction defects with powdery mildew
susceptibility in Arabidopsis enhanced disease susceptibility
mutants.";
Plant J. 16:473-485(1998).
[10]
FUNCTION, AND DISRUPTION PHENOTYPE.
STRAIN=cv. Columbia;
PubMed=10796016; DOI=10.1094/MPMI.2000.13.5.503;
Gupta V., Willits M.G., Glazebrook J.;
"Arabidopsis thaliana EDS4 contributes to salicylic acid (SA)-
dependent expression of defense responses: evidence for inhibition of
jasmonic acid signaling by SA.";
Mol. Plant Microbe Interact. 13:503-511(2000).
[11]
FUNCTION, AND DISRUPTION PHENOTYPE.
STRAIN=cv. Columbia;
PubMed=11041879; DOI=10.1105/tpc.12.10.1823;
Asai T., Stone J.M., Heard J.E., Kovtun Y., Yorgey P., Sheen J.,
Ausubel F.M.;
"Fumonisin B1-induced cell death in arabidopsis protoplasts requires
jasmonate-, ethylene-, and salicylate-dependent signaling pathways.";
Plant Cell 12:1823-1836(2000).
[12]
FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH EDS1, AND INDUCTION
BY SALICYLIC ACID AND PATHOGENS.
PubMed=11574472; DOI=10.1093/emboj/20.19.5400;
Feys B.J., Moisan L.J., Newman M.-A., Parker J.E.;
"Direct interaction between the Arabidopsis disease resistance
signaling proteins, EDS1 and PAD4.";
EMBO J. 20:5400-5411(2001).
[13]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=11595797; DOI=10.1105/tpc.13.10.2211;
Rusterucci C., Aviv D.H., Holt B.F. III, Dangl J.L., Parker J.E.;
"The disease resistance signaling components EDS1 and PAD4 are
essential regulators of the cell death pathway controlled by LSD1 in
Arabidopsis.";
Plant Cell 13:2211-2224(2001).
[14]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=11826312; DOI=10.1105/tpc.010376;
Nawrath C., Heck S., Parinthawong N., Metraux J.-P.;
"EDS5, an essential component of salicylic acid-dependent signaling
for disease resistance in Arabidopsis, is a member of the MATE
transporter family.";
Plant Cell 14:275-286(2002).
[15]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=11846877; DOI=10.1046/j.0960-7412.2001.01229.x;
van der Biezen E.A., Freddie C.T., Kahn K., Parker J.E., Jones J.D.;
"Arabidopsis RPP4 is a member of the RPP5 multigene family of TIR-NB-
LRR genes and confers downy mildew resistance through multiple
signalling components.";
Plant J. 29:439-451(2002).
[16]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=14617091; DOI=10.1046/j.1365-313X.2003.01881.x;
Heck S., Grau T., Buchala A., Metraux J.P., Nawrath C.;
"Genetic evidence that expression of NahG modifies defence pathways
independent of salicylic acid biosynthesis in the Arabidopsis-
Pseudomonas syringae pv. tomato interaction.";
Plant J. 36:342-352(2003).
[17]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=15447647; DOI=10.1111/j.1365-313X.2004.02200.x;
Song J.T., Lu H., McDowell J.M., Greenberg J.T.;
"A key role for ALD1 in activation of local and systemic defenses in
Arabidopsis.";
Plant J. 40:200-212(2004).
[18]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=15347794; DOI=10.1104/pp.104.043646;
Mateo A., Muhlenbock P., Rusterucci C., Chang C.C., Miszalski Z.,
Karpinska B., Parker J.E., Mullineaux P.M., Karpinski S.;
"LESION SIMULATING DISEASE 1 is required for acclimation to conditions
that promote excess excitation energy.";
Plant Physiol. 136:2818-2830(2004).
[19]
REVIEW.
PubMed=15939664; DOI=10.1016/j.pbi.2005.05.010;
Wiermer M., Feys B.J., Parker J.E.;
"Plant immunity: the EDS1 regulatory node.";
Curr. Opin. Plant Biol. 8:383-389(2005).
[20]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=16353557; DOI=10.1094/MPMI-18-1226;
McDowell J.M., Williams S.G., Funderburg N.T., Eulgem T., Dangl J.L.;
"Genetic analysis of developmentally regulated resistance to downy
mildew (Hyaloperonospora parasitica) in Arabidopsis thaliana.";
Mol. Plant Microbe Interact. 18:1226-1234(2005).
[21]
FUNCTION, INTERACTION WITH EDS1, AND SUBCELLULAR LOCATION.
PubMed=16040633; DOI=10.1105/tpc.105.033910;
Feys B.J., Wiermer M., Bhat R.A., Moisan L.J., Medina-Escobar N.,
Neu C., Cabral A., Parker J.E.;
"Arabidopsis SENESCENCE-ASSOCIATED GENE101 stabilizes and signals
within an ENHANCED DISEASE SUSCEPTIBILITY1 complex in plant innate
immunity.";
Plant Cell 17:2601-2613(2005).
[22]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=15773856; DOI=10.1111/j.1365-313X.2005.02356.x;
Xiao S., Calis O., Patrick E., Zhang G., Charoenwattana P.,
Muskett P., Parker J.E., Turner J.G.;
"The atypical resistance gene, RPW8, recruits components of basal
defence for powdery mildew resistance in Arabidopsis.";
Plant J. 42:95-110(2005).
[23]
FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY GREEN PEACH APHID.
PubMed=16299172; DOI=10.1104/pp.105.070433;
Pegadaraju V., Knepper C., Reese J., Shah J.;
"Premature leaf senescence modulated by the Arabidopsis PHYTOALEXIN
DEFICIENT4 gene is associated with defense against the phloem-feeding
green peach aphid.";
Plant Physiol. 139:1927-1934(2005).
[24]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=16813576; DOI=10.1111/j.1365-313X.2006.02806.x;
Brodersen P., Petersen M., Bjorn Nielsen H., Zhu S., Newman M.A.,
Shokat K.M., Rietz S., Parker J., Mundy J.;
"Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic
acid/ethylene-dependent responses via EDS1 and PAD4.";
Plant J. 47:532-546(2006).
[25]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=18055613; DOI=10.1105/tpc.106.048843;
Muehlenbock P., Plaszczyca M., Plaszczyca M., Mellerowicz E.,
Karpinski S.;
"Lysigenous aerenchyma formation in Arabidopsis is controlled by
LESION SIMULATING DISEASE1.";
Plant Cell 19:3819-3830(2007).
[26]
FUNCTION, AND DISRUPTION PHENOTYPE.
STRAIN=cv. Columbia, cv. Landsberg erecta, and cv. Wassilewskija;
PubMed=17725549; DOI=10.1111/j.1365-313X.2007.03241.x;
Pegadaraju V., Louis J., Singh V., Reese J.C., Bautor J., Feys B.J.,
Cook G., Parker J.E., Shah J.;
"Phloem-based resistance to green peach aphid is controlled by
Arabidopsis PHYTOALEXIN DEFICIENT4 without its signaling partner
ENHANCED DISEASE SUSCEPTIBILITY1.";
Plant J. 52:332-341(2007).
[27]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=17431038; DOI=10.1073/pnas.0609259104;
Chandra-Shekara A.C., Venugopal S.C., Barman S.R., Kachroo A.,
Kachroo P.;
"Plastidial fatty acid levels regulate resistance gene-dependent
defense signaling in Arabidopsis.";
Proc. Natl. Acad. Sci. U.S.A. 104:7277-7282(2007).
[28]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=18005228; DOI=10.1111/j.1365-313X.2007.03369.x;
Tsuda K., Sato M., Glazebrook J., Cohen J.D., Katagiri F.;
"Interplay between MAMP-triggered and SA-mediated defense responses.";
Plant J. 53:763-775(2008).
[29]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=18266921; DOI=10.1111/j.1365-313X.2008.03439.x;
Lee M.W., Jelenska J., Greenberg J.T.;
"Arabidopsis proteins important for modulating defense responses to
Pseudomonas syringae that secrete HopW1-1.";
Plant J. 54:452-465(2008).
[30]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=20367470; DOI=10.1094/MPMI-23-5-0618;
Louis J., Leung Q., Pegadaraju V., Reese J., Shah J.;
"PAD4-dependent antibiosis contributes to the ssi2-conferred hyper-
resistance to the green peach aphid.";
Mol. Plant Microbe Interact. 23:618-627(2010).
[31]
FUNCTION, INTERACTION WITH EDS1, INDUCTION BY HYALOPERONOSPORA
ARABIDOPSIDIS, AND SUBCELLULAR LOCATION.
STRAIN=cv. Wassilewskija;
PubMed=21434927; DOI=10.1111/j.1469-8137.2011.03675.x;
Rietz S., Stamm A., Malonek S., Wagner S., Becker D.,
Medina-Escobar N., Vlot A.C., Feys B.J., Niefind K., Parker J.E.;
"Different roles of Enhanced Disease Susceptibility1 (EDS1) bound to
and dissociated from Phytoalexin Deficient4 (PAD4) in Arabidopsis
immunity.";
New Phytol. 191:107-119(2011).
[32]
INDUCTION BY GREEN PEACH APHID AND TREHALOSE.
PubMed=21426427; DOI=10.1111/j.1365-313X.2011.04583.x;
Singh V., Louis J., Ayre B.G., Reese J.C., Pegadaraju V., Shah J.;
"TREHALOSE PHOSPHATE SYNTHASE11-dependent trehalose metabolism
promotes Arabidopsis thaliana defense against the phloem-feeding
insect Myzus persicae.";
Plant J. 67:94-104(2011).
[33]
FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, AND INTERACTION WITH EDS1.
PubMed=22072959; DOI=10.1371/journal.ppat.1002318;
Zhu S., Jeong R.-D., Venugopal S.C., Lapchyk L., Navarre D.,
Kachroo A., Kachroo P.;
"SAG101 forms a ternary complex with EDS1 and PAD4 and is required for
resistance signaling against turnip crinkle virus.";
PLoS Pathog. 7:E1002318-E1002318(2011).
[34]
FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH VICTR, AND SUBUNIT.
STRAIN=cv. Columbia;
PubMed=23275581; DOI=10.1105/tpc.112.107235;
Kim T.H., Kunz H.H., Bhattacharjee S., Hauser F., Park J.,
Engineer C., Liu A., Ha T., Parker J.E., Gassmann W., Schroeder J.I.;
"Natural variation in small molecule-induced TIR-NB-LRR signaling
induces root growth arrest via EDS1- and PAD4-complexed R protein
VICTR in Arabidopsis.";
Plant Cell 24:5177-5192(2012).
[35]
FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF SER-118.
PubMed=22353573; DOI=10.1104/pp.112.193417;
Louis J., Gobbato E., Mondal H.A., Feys B.J., Parker J.E., Shah J.;
"Discrimination of Arabidopsis PAD4 activities in defense against
green peach aphid and pathogens.";
Plant Physiol. 158:1860-1872(2012).
[36]
INDUCTION BY GREEN PEACH APHID.
PubMed=22990443; DOI=10.4161/psb.22088;
Louis J., Mondal H.A., Shah J.;
"Green peach aphid infestation induces Arabidopsis PHYTOALEXIN-
DEFICIENT4 expression at site of insect feeding.";
Plant Signal. Behav. 7:1431-1433(2012).
[37]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=23400705; DOI=10.1104/pp.112.208116;
Wituszynska W., Slesak I., Vanderauwera S., Szechynska-Hebda M.,
Kornas A., Van Der Kelen K., Muhlenbock P., Karpinska B.,
Mackowski S., Van Breusegem F., Karpinski S.;
"Lesion simulating disease1, enhanced disease susceptibility1, and
phytoalexin deficient4 conditionally regulate cellular signaling
homeostasis, photosynthesis, water use efficiency, and seed yield in
Arabidopsis.";
Plant Physiol. 161:1795-1805(2013).
[38]
INTERACTION WITH EDS1, 3D-STRUCTURE MODELING, AND MUTAGENESIS OF
MET-16; LEU-21 AND PHE-143.
PubMed=24331460; DOI=10.1016/j.chom.2013.11.006;
Wagner S., Stuttmann J., Rietz S., Guerois R., Brunstein E.,
Bautor J., Niefind K., Parker J.E.;
"Structural basis for signaling by exclusive EDS1 heteromeric
complexes with SAG101 or PAD4 in plant innate immunity.";
Cell Host Microbe 14:619-630(2013).
-!- FUNCTION: Probable lipase required downstream of MPK4 for
accumulation of the plant defense-potentiating molecule, salicylic
acid, thus contributing to the plant innate immunity against
invasive biotrophic pathogens and to defense mechanisms upon
recognition of microbe-associated molecular patterns (MAMPs).
Participates in the regulation of various molecular and
physiological processes that influence fitness. Together with
SG101, required for programmed cell death (PCD) triggered by NBS-
LRR resistance proteins (e.g. RPS4, RPW8.1 and RPW8.2) in response
to the fungal toxin fumonisin B1 (FB1) and avirulent pathogens
(e.g. P.syringae pv. tomato strain DC3000 avrRps4 and pv.
maculicola, turnip crinkle virus (TCV), and H.arabidopsidis
isolates CALA2, EMOY2, EMWA1 and HIND4). Together with EDS1,
confers a basal resistance by restricting the growth of virulent
pathogens (e.g. H.arabidopsidis isolates NOCO2 and EMCO5,
E.orontii isolate MGH, and P.syringae pv. tomato strain DC3000 or
expressing HopW1-1 (HopPmaA)). Necessary for the salicylic acid-
(SA-) dependent systemic acquired resistance (SAR) response that
involves expression of multiple defense responses, including
synthesis of the phytoalexin camalexin and expression of
pathogenesis-related genes (e.g. PR1, ALD1, BGL2 and PR5) in
response to pathogens, triggering a signal amplification loop that
increases SA levels via EDS5 and SID2, but, together with EDS1,
seems to repress the ethylene/jasmonic acid (ET/JA) defense
pathway. May also function in response to abiotic stresses such as
UV-C light and LSD1-dependent acclimatization to light conditions
that promote excess excitation energy (EEE), probably by
transducing redox signals and modulating stomatal conductance.
Regulates the formation of lysigenous aerenchyma in hypocotyls in
response to hypoxia, maybe via hydrogen peroxide production.
Modulates leaf senescence in insect-infested tissue and triggers a
phloem-based defense mechanism including antibiosis (e.g. green
peach aphid (GPA), M.persicae) to limit phloem sap uptake and
insect growth, thus providing an EDS1-independent basal resistance
to insects. Also involved in regulation of root meristematic zone-
targeted growth arrest together with EDS1 and in a VICTR-dependent
manner. {ECO:0000269|PubMed:10557364, ECO:0000269|PubMed:10796016,
ECO:0000269|PubMed:11041879, ECO:0000269|PubMed:11574472,
ECO:0000269|PubMed:11595797, ECO:0000269|PubMed:11826312,
ECO:0000269|PubMed:11846877, ECO:0000269|PubMed:14617091,
ECO:0000269|PubMed:15347794, ECO:0000269|PubMed:15447647,
ECO:0000269|PubMed:15773856, ECO:0000269|PubMed:16040633,
ECO:0000269|PubMed:16299172, ECO:0000269|PubMed:16353557,
ECO:0000269|PubMed:16813576, ECO:0000269|PubMed:17431038,
ECO:0000269|PubMed:17725549, ECO:0000269|PubMed:18005228,
ECO:0000269|PubMed:18055613, ECO:0000269|PubMed:18266921,
ECO:0000269|PubMed:20367470, ECO:0000269|PubMed:21434927,
ECO:0000269|PubMed:22072959, ECO:0000269|PubMed:22353573,
ECO:0000269|PubMed:23275581, ECO:0000269|PubMed:23400705,
ECO:0000269|PubMed:8725243, ECO:0000269|PubMed:9136026,
ECO:0000269|PubMed:9634589, ECO:0000269|PubMed:9881167}.
-!- SUBUNIT: Part of a nuclear complex made of EDS1, SG101 and PAD4
that can be redirected to the cytoplasm in the presence of an
extranuclear form of EDS1. Sabilized by direct interaction with
EDS1 in infected leaves. Part of a nuclear protein complex made of
VICTR, PAD4 and EDS1 (PubMed:23275581). Interacts with VICTR
(PubMed:23275581). Interacts with EDS1 (PubMed:24331460).
{ECO:0000269|PubMed:11574472, ECO:0000269|PubMed:16040633,
ECO:0000269|PubMed:21434927, ECO:0000269|PubMed:22072959,
ECO:0000269|PubMed:23275581, ECO:0000269|PubMed:24331460}.
-!- INTERACTION:
Q9SU72:EDS1; NbExp=4; IntAct=EBI-1390441, EBI-1390454;
-!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Can move to the
cytoplasm when in complex with EDS1.
-!- INDUCTION: By benzothiadiazole (BTH), at site of green peach aphid
feeding (GPA, M.persicae) via TPS11-dependent trehalose
accumulation, and H.arabidopsidis. Induced by P.syringae in a
NPR1-independent manner, and by salicylic acid (SA) in a NPR1-
dependent manner. {ECO:0000269|PubMed:10557364,
ECO:0000269|PubMed:11574472, ECO:0000269|PubMed:16299172,
ECO:0000269|PubMed:21426427, ECO:0000269|PubMed:21434927,
ECO:0000269|PubMed:22990443}.
-!- DISRUPTION PHENOTYPE: Impaired camalexin accumulation, reduced
synthesis of salicylic acid (SA) and ethylene (ET), and altered
expression of pathogenesis-related genes (e.g. PR1, ALD1, BGL2 and
PR5) upon some pathogenic infections (e.g. P.syringae) and
microbe-associated molecular patterns (MAMPs) recognition. Loss of
the systemic acquired resistance response. Reduced fitness
characterized by lower seed yield and survival rate. Increased
sensitivity to P.syringae, H.arabidopsidis, turnip crinkle virus
(TCV) and E.orontii. These phenotypes are reversed by SA
treatment. Altered sensitivity to jasmonic acid (JA) and ethylene
(ET) signaling. Decreased susceptibility to the fungal toxin
fumonisin B1 (FB1) that mediates programmed cell death (PCD).
Impaired induction of EDS5/SID1 expression after UV-C light
exposure and pathogen attack. Altered LSD1-dependent
acclimatization to light conditions that promote excess excitation
energy (EEE). Impaired formation of lysigenous aerenchyma in
response to hypoxia. Reduced resistance against green peach aphid
(GPA, M.persicae) due to increased phloem sap uptake, reduced
accumulation of antibiotic activity in petiole exudates, and
delayed leaf senescence in insect-infested tissue, including
chlorophyll loss, cell death, and senescence associated genes
(SAG) expression. Loss of [5-(3,4-dichlorophenyl)furan-2-yl]-
piperidine-1-ylmethanethione- (DFPM-) induced root growth arrest
and inhibition of stomatal closing mediated by abscisic acid
(ABA). {ECO:0000269|PubMed:10557364, ECO:0000269|PubMed:10796016,
ECO:0000269|PubMed:11041879, ECO:0000269|PubMed:11574472,
ECO:0000269|PubMed:11595797, ECO:0000269|PubMed:11826312,
ECO:0000269|PubMed:11846877, ECO:0000269|PubMed:14617091,
ECO:0000269|PubMed:15347794, ECO:0000269|PubMed:15447647,
ECO:0000269|PubMed:15773856, ECO:0000269|PubMed:16299172,
ECO:0000269|PubMed:16353557, ECO:0000269|PubMed:16813576,
ECO:0000269|PubMed:17431038, ECO:0000269|PubMed:17725549,
ECO:0000269|PubMed:18005228, ECO:0000269|PubMed:18055613,
ECO:0000269|PubMed:18266921, ECO:0000269|PubMed:20367470,
ECO:0000269|PubMed:22353573, ECO:0000269|PubMed:23275581,
ECO:0000269|PubMed:23400705, ECO:0000269|PubMed:8725243,
ECO:0000269|PubMed:9136026, ECO:0000269|PubMed:9634589,
ECO:0000269|PubMed:9881167}.
-!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase
family. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; AF188329; AAF09479.1; -; mRNA.
EMBL; EF470727; ABR46037.1; -; Genomic_DNA.
EMBL; EF470728; ABR46038.1; -; Genomic_DNA.
EMBL; EF470729; ABR46039.1; -; Genomic_DNA.
EMBL; EF470731; ABR46041.1; -; Genomic_DNA.
EMBL; EF470733; ABR46043.1; -; Genomic_DNA.
EMBL; EF470735; ABR46045.1; -; Genomic_DNA.
EMBL; EF470736; ABR46046.1; -; Genomic_DNA.
EMBL; EF470737; ABR46047.1; -; Genomic_DNA.
EMBL; EF470741; ABR46051.1; -; Genomic_DNA.
EMBL; EF470742; ABR46052.1; -; Genomic_DNA.
EMBL; EF470743; ABR46053.1; -; Genomic_DNA.
EMBL; AL050300; CAB43438.1; -; Genomic_DNA.
EMBL; CP002686; AEE78945.1; -; Genomic_DNA.
EMBL; EU405144; ABZ02805.1; -; Genomic_DNA.
EMBL; EU405145; ABZ02806.1; -; Genomic_DNA.
EMBL; EU405146; ABZ02807.1; -; Genomic_DNA.
EMBL; EU405149; ABZ02810.1; -; Genomic_DNA.
EMBL; EU405150; ABZ02811.1; -; Genomic_DNA.
EMBL; EU405152; ABZ02813.1; -; Genomic_DNA.
EMBL; EU405153; ABZ02814.1; -; Genomic_DNA.
EMBL; EU405155; ABZ02816.1; -; Genomic_DNA.
EMBL; EU405159; ABZ02820.1; -; Genomic_DNA.
EMBL; EU405161; ABZ02822.1; -; Genomic_DNA.
EMBL; EU405162; ABZ02823.1; -; Genomic_DNA.
EMBL; EU405163; ABZ02824.1; -; Genomic_DNA.
EMBL; EU405164; ABZ02825.1; -; Genomic_DNA.
EMBL; EU405173; ABZ02834.1; -; Genomic_DNA.
EMBL; EU405174; ABZ02835.1; -; Genomic_DNA.
EMBL; EU405175; ABZ02836.1; -; Genomic_DNA.
EMBL; EU405177; ABZ02838.1; -; Genomic_DNA.
EMBL; EU405180; ABZ02841.1; -; Genomic_DNA.
EMBL; EU405181; ABZ02842.1; -; Genomic_DNA.
EMBL; EU405183; ABZ02844.1; -; Genomic_DNA.
EMBL; EU405188; ABZ02849.1; -; Genomic_DNA.
EMBL; EU405190; ABZ02851.1; -; Genomic_DNA.
EMBL; EU405192; ABZ02853.1; -; Genomic_DNA.
EMBL; EU405193; ABZ02854.1; -; Genomic_DNA.
EMBL; EU405194; ABZ02855.1; -; Genomic_DNA.
EMBL; EU405195; ABZ02856.1; -; Genomic_DNA.
EMBL; EU405197; ABZ02858.1; -; Genomic_DNA.
EMBL; EU405205; ABZ02866.1; -; Genomic_DNA.
EMBL; EU405206; ABZ02867.1; -; Genomic_DNA.
EMBL; EU405208; ABZ02869.1; -; Genomic_DNA.
EMBL; EU405209; ABZ02870.1; -; Genomic_DNA.
EMBL; EU405210; ABZ02871.1; -; Genomic_DNA.
EMBL; EU405212; ABZ02873.1; -; Genomic_DNA.
EMBL; EU405213; ABZ02874.1; -; Genomic_DNA.
EMBL; EU405214; ABZ02875.1; -; Genomic_DNA.
EMBL; EU405215; ABZ02876.1; -; Genomic_DNA.
EMBL; EU405217; ABZ02878.1; -; Genomic_DNA.
EMBL; EU405219; ABZ02880.1; -; Genomic_DNA.
EMBL; EU405223; ABZ02884.1; -; Genomic_DNA.
EMBL; EU405224; ABZ02885.1; -; Genomic_DNA.
EMBL; EU405228; ABZ02889.1; -; Genomic_DNA.
EMBL; EU405231; ABZ02892.1; -; Genomic_DNA.
EMBL; EU405232; ABZ02893.1; -; Genomic_DNA.
EMBL; EU405237; ABZ02898.1; -; Genomic_DNA.
PIR; T08456; T08456.
RefSeq; NP_190811.1; NM_115103.4.
UniGene; At.22858; -.
UniGene; At.69183; -.
UniGene; At.69581; -.
ProteinModelPortal; Q9S745; -.
SMR; Q9S745; -.
BioGrid; 9726; 2.
IntAct; Q9S745; 1.
STRING; 3702.AT3G52430.1; -.
ESTHER; arath-PAD4; Plant_lipase_EDS1-like.
PaxDb; Q9S745; -.
PRIDE; Q9S745; -.
EnsemblPlants; AT3G52430.1; AT3G52430.1; AT3G52430.
GeneID; 824408; -.
Gramene; AT3G52430.1; AT3G52430.1; AT3G52430.
KEGG; ath:AT3G52430; -.
Araport; AT3G52430; -.
TAIR; locus:2079939; AT3G52430.
eggNOG; ENOG410IJZD; Eukaryota.
eggNOG; ENOG410Y7YA; LUCA.
InParanoid; Q9S745; -.
OMA; VEEWNQL; -.
OrthoDB; EOG093606VH; -.
PhylomeDB; Q9S745; -.
PRO; PR:Q9S745; -.
Proteomes; UP000006548; Chromosome 3.
ExpressionAtlas; Q9S745; baseline and differential.
Genevisible; Q9S745; AT.
GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
GO; GO:0005634; C:nucleus; IDA:UniProtKB.
GO; GO:0016298; F:lipase activity; ISS:TAIR.
GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
GO; GO:0010618; P:aerenchyma formation; IMP:TAIR.
GO; GO:0071327; P:cellular response to trehalose stimulus; IDA:UniProtKB.
GO; GO:0009816; P:defense response to bacterium, incompatible interaction; IMP:UniProtKB.
GO; GO:0002213; P:defense response to insect; IMP:TAIR.
GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
GO; GO:0010150; P:leaf senescence; IMP:TAIR.
GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
GO; GO:0031348; P:negative regulation of defense response; IMP:TAIR.
GO; GO:0010105; P:negative regulation of ethylene-activated signaling pathway; IGI:UniProtKB.
GO; GO:0009626; P:plant-type hypersensitive response; IEA:UniProtKB-KW.
GO; GO:1901183; P:positive regulation of camalexin biosynthetic process; IMP:UniProtKB.
GO; GO:0010942; P:positive regulation of cell death; IMP:UniProtKB.
GO; GO:1900426; P:positive regulation of defense response to bacterium; IMP:UniProtKB.
GO; GO:1900367; P:positive regulation of defense response to insect; IMP:UniProtKB.
GO; GO:0080151; P:positive regulation of salicylic acid mediated signaling pathway; IGI:UniProtKB.
GO; GO:0010310; P:regulation of hydrogen peroxide metabolic process; IMP:TAIR.
GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IGI:UniProtKB.
GO; GO:0080142; P:regulation of salicylic acid biosynthetic process; IMP:UniProtKB.
GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IMP:UniProtKB.
GO; GO:0009617; P:response to bacterium; IDA:UniProtKB.
GO; GO:0001666; P:response to hypoxia; IMP:TAIR.
GO; GO:0009625; P:response to insect; IDA:UniProtKB.
GO; GO:0051707; P:response to other organism; IEP:TAIR.
GO; GO:0009751; P:response to salicylic acid; IDA:UniProtKB.
GO; GO:0010225; P:response to UV-C; IMP:UniProtKB.
GO; GO:0009627; P:systemic acquired resistance; IEP:TAIR.
GO; GO:0009862; P:systemic acquired resistance, salicylic acid mediated signaling pathway; IMP:UniProtKB.
Gene3D; 3.40.50.1820; -; 1.
InterPro; IPR029058; AB_hydrolase.
InterPro; IPR002921; Fungal_lipase-like.
Pfam; PF01764; Lipase_3; 1.
SUPFAM; SSF53474; SSF53474; 1.
1: Evidence at protein level;
Complete proteome; Cytoplasm; Ethylene signaling pathway; Hydrolase;
Hypersensitive response; Jasmonic acid signaling pathway;
Lipid degradation; Lipid metabolism; Nucleus; Plant defense;
Reference proteome; Transferase.
CHAIN 1 541 Lipase-like PAD4.
/FTId=PRO_0000429488.
ACT_SITE 118 118 Nucleophile.
{ECO:0000250|UniProtKB:P19515}.
ACT_SITE 178 178 Charge relay system.
{ECO:0000250|UniProtKB:P19515}.
ACT_SITE 229 229 Charge relay system.
{ECO:0000250|UniProtKB:P19515}.
MUTAGEN 16 16 M->A: Loss of interaction with EDS1; when
associated with S-21. Loss of interaction
with EDS1; when associated with S-21 and
A-143. {ECO:0000269|PubMed:24331460}.
MUTAGEN 21 21 L->S: Loss of interaction with EDS1; when
associated with A-16. Loss of interaction
with EDS1; when associated with A-16 and
A-143. {ECO:0000269|PubMed:24331460}.
MUTAGEN 118 118 S->A: Loss of antibiosis and deterrence
against green peach aphid (GPA,
M.persicae) feeding, but normal leaf
senescence and plant defense against
pathogens. {ECO:0000269|PubMed:22353573}.
MUTAGEN 143 143 F->A: Loss of interaction with EDS1; when
associated with A-16 and S-21.
{ECO:0000269|PubMed:24331460}.
SEQUENCE 541 AA; 60985 MW; D6D5D3EBB522C11A CRC64;
MDDCRFETSE LQASVMISTP LFTDSWSSCN TANCNGSIKI HDIAGITYVA IPAVSMIQLG
NLVGLPVTGD VLFPGLSSDE PLPMVDAAIL KLFLQLKIKE GLELELLGKK LVVITGHSTG
GALAAFTALW LLSQSSPPSF RVFCITFGSP LLGNQSLSTS ISRSRLAHNF CHVVSIHDLV
PRSSNEQFWP FGTYLFCSDK GGVCLDNAGS VRLMFNILNT TATQNTEEHQ RYGHYVFTLS
HMFLKSRSFL GGSIPDNSYQ AGVALAVEAL GFSNDDTSGV LVKECIETAT RIVRAPILRS
AELANELASV LPARLEIQWY KDRCDASEEQ LGYYDFFKRY SLKRDFKVNM SRIRLAKFWD
TVIKMVETNE LPFDFHLGKK WIYASQFYQL LAEPLDIANF YKNRDIKTGG HYLEGNRPKR
YEVIDKWQKG VKVPEECVRS RYASTTQDTC FWAKLEQAKE WLDEARKESS DPQRRSLLRE
KIVPFESYAN TLVTKKEVSL DVKAKNSSYS VWEANLKEFK CKMGYENEIE MVVDESDAME
T


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