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Low-molecular weight cobalt-containing nitrile hydratase subunit beta (L-NHase) (L-nitrilase) (EC 4.2.1.84)

 NHB2_RHORH              Reviewed;         226 AA.
P29379;
01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
01-DEC-1992, sequence version 1.
22-NOV-2017, entry version 68.
RecName: Full=Low-molecular weight cobalt-containing nitrile hydratase subunit beta;
Short=L-NHase;
Short=L-nitrilase;
EC=4.2.1.84;
Rhodococcus rhodochrous.
Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae;
Rhodococcus.
NCBI_TaxID=1829;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=J1;
PubMed=1840499; DOI=10.1016/0167-4781(91)90208-4;
Kobayashi M., Nishiyama M., Nagasawa T., Horinouchi S., Beppu T.,
Yamada H.;
"Cloning, nucleotide sequence and expression in Escherichia coli of
two cobalt-containing nitrile hydratase genes from Rhodococcus
rhodochrous J1.";
Biochim. Biophys. Acta 1129:23-33(1991).
-!- FUNCTION: NHase catalyzes the hydration of various nitrile
compounds to the corresponding amides.
-!- CATALYTIC ACTIVITY: An aliphatic amide = a nitrile + H(2)O.
-!- SUBUNIT: Heterodimer of an alpha and a beta chain.
-!- INTERACTION:
P29378:-; NbExp=2; IntAct=EBI-15729954, EBI-15729943;
-!- INDUCTION: By cobalt and urea or cyclohexanecarboxamide.
-!- BIOTECHNOLOGY: Industrial production of acrylamide is now being
developed using some of these enzymes.
-!- SIMILARITY: Belongs to the nitrile hydratase subunit beta family.
{ECO:0000305}.
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EMBL; X64360; CAA45711.1; -; Genomic_DNA.
PIR; S19715; S19715.
ProteinModelPortal; P29379; -.
SMR; P29379; -.
DIP; DIP-46300N; -.
IntAct; P29379; 1.
GO; GO:0080109; F:indole-3-acetonitrile nitrile hydratase activity; IEA:UniProtKB-EC.
GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
InterPro; IPR024690; CN_hydtase_beta_dom.
InterPro; IPR008990; Elect_transpt_acc-like_dom_sf.
InterPro; IPR003168; Nitrile_hydratase_bsu.
Pfam; PF02211; NHase_beta; 1.
PIRSF; PIRSF001427; NHase_beta; 1.
SUPFAM; SSF50090; SSF50090; 1.
TIGRFAMs; TIGR03888; nitrile_beta; 1.
1: Evidence at protein level;
Direct protein sequencing; Lyase.
CHAIN 1 226 Low-molecular weight cobalt-containing
nitrile hydratase subunit beta.
/FTId=PRO_0000186833.
SEQUENCE 226 AA; 25201 MW; A790BCC67B319654 CRC64;
MDGIHDLGGR AGLGPIKPES DEPVFHSDWE RSVLTMFPAM ALAGAFNLDQ FRGAMEQIPP
HDYLTSQYYE HWMHAMIHHG IEAGIFDSDE LDRRTQYYMD HPDDTTPTRQ DPQLVETISQ
LITHGADYRR PTDTEAAFAV GDKVIVRSDA SPNTHTRRAG YVRGRVGEVV ATHGAYVFPD
TNALGAGESP EHLYTVRFSA TELWGEPAAP NVVNHIDVFE PYLLPA


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