Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Magnesium-chelatase subunit ChlI-2, chloroplastic (Mg-chelatase subunit I-2) (EC 6.6.1.1) (Mg-protoporphyrin IX chelatase subunit ChlI-2)

 CHLI2_ARATH             Reviewed;         418 AA.
Q5XF33; Q8L712; Q9FJ43;
05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
23-NOV-2004, sequence version 1.
18-JUL-2018, entry version 114.
RecName: Full=Magnesium-chelatase subunit ChlI-2, chloroplastic;
Short=Mg-chelatase subunit I-2;
EC=6.6.1.1;
AltName: Full=Mg-protoporphyrin IX chelatase subunit ChlI-2;
Flags: Precursor;
Name=CHLI2; OrderedLocusNames=At5g45930; ORFNames=K15I22.13;
Arabidopsis thaliana (Mouse-ear cress).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
Arabidopsis.
NCBI_TaxID=3702;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
PubMed=10048488; DOI=10.1093/dnares/5.6.379;
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. VIII.
Sequence features of the regions of 1,081,958 bp covered by seventeen
physically assigned P1 and TAC clones.";
DNA Res. 5:379-391(1998).
[2]
GENOME REANNOTATION.
STRAIN=cv. Columbia;
PubMed=27862469; DOI=10.1111/tpj.13415;
Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
Town C.D.;
"Araport11: a complete reannotation of the Arabidopsis thaliana
reference genome.";
Plant J. 89:789-804(2017).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
PubMed=14593172; DOI=10.1126/science.1088305;
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis
genome.";
Science 302:842-846(2003).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
"Arabidopsis ORF clones.";
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
[5]
TISSUE SPECIFICITY.
PubMed=11842180; DOI=10.1104/pp.128.2.770;
Rissler H.M., Collakova E., DellaPenna D., Whelan J., Pogson B.J.;
"Chlorophyll biosynthesis. Expression of a second chl I gene of
magnesium chelatase in Arabidopsis supports only limited chlorophyll
synthesis.";
Plant Physiol. 128:770-779(2002).
[6]
FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, ENZYME REGULATION,
BIOPHYSICOCHEMICAL PROPERTIES, AND DISRUPTION PHENOTYPE.
PubMed=18846282; DOI=10.1039/b802604c;
Kobayashi K., Mochizuki N., Yoshimura N., Motohashi K., Hisabori T.,
Masuda T.;
"Functional analysis of Arabidopsis thaliana isoforms of the Mg-
chelatase CHLI subunit.";
Photochem. Photobiol. Sci. 7:1188-1195(2008).
[7]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=19363094; DOI=10.1104/pp.109.135368;
Huang Y.S., Li H.M.;
"Arabidopsis CHLI2 can substitute for CHLI1.";
Plant Physiol. 150:636-645(2009).
[8]
ACETYLATION [LARGE SCALE ANALYSIS] AT VAL-56, CLEAVAGE OF TRANSIT
PEPTIDE [LARGE SCALE ANALYSIS] AFTER SER-55, AND IDENTIFICATION BY
MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
PubMed=22223895; DOI=10.1074/mcp.M111.015131;
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C.,
Meinnel T., Giglione C.;
"Comparative large-scale characterisation of plant vs. mammal proteins
reveals similar and idiosyncratic N-alpha acetylation features.";
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
-!- FUNCTION: Involved in chlorophyll biosynthesis. Catalyzes the
insertion of magnesium ion into protoporphyrin IX to yield Mg-
protoporphyrin IX. The reaction takes place in two steps, with an
ATP-dependent activation followed by an ATP-dependent chelation
step. Possesses low affinity for ATP and may play a limited role
in chlorophyll biosynthesis, and contributes to the assembly of
the Mg-chelatase complex. {ECO:0000269|PubMed:18846282,
ECO:0000269|PubMed:19363094}.
-!- CATALYTIC ACTIVITY: ATP + protoporphyrin IX + Mg(2+) + H(2)O = ADP
+ phosphate + Mg-protoporphyrin IX + 2 H(+).
-!- ENZYME REGULATION: Redox regulation; active in reducing
conditions, inactive in oxidizing conditions. Thioredoxins f and m
mediate the reversible reductive activation of oxidized CHLI2.
{ECO:0000269|PubMed:18846282}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=1.29 mM for ATP {ECO:0000269|PubMed:18846282};
Vmax=27.8 nmol/min/mg enzyme toward ATP
{ECO:0000269|PubMed:18846282};
-!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
biosynthesis.
-!- SUBUNIT: The magnesium chelatase complex is a heterotrimer
consisting of subunits CHLI, CHLD, AND CHLH.
{ECO:0000269|PubMed:18846282}.
-!- SUBCELLULAR LOCATION: Plastid, chloroplast
{ECO:0000305|PubMed:18846282}.
-!- TISSUE SPECIFICITY: Expressed in leaves.
{ECO:0000269|PubMed:11842180}.
-!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
conditions, but plants show slight decrease in chlorophyll
content. Chli1 and chli2 double mutants are albino.
{ECO:0000269|PubMed:18846282, ECO:0000269|PubMed:19363094}.
-!- SIMILARITY: Belongs to the Mg-chelatase subunits D/I family.
{ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=BAB09321.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; AB016870; BAB09321.1; ALT_SEQ; Genomic_DNA.
EMBL; CP002688; AED95316.1; -; Genomic_DNA.
EMBL; AY140022; AAM98163.1; -; mRNA.
EMBL; BT015783; AAU90073.1; -; mRNA.
RefSeq; NP_199405.2; NM_123961.4.
UniGene; At.29985; -.
UniGene; At.70220; -.
ProteinModelPortal; Q5XF33; -.
SMR; Q5XF33; -.
STRING; 3702.AT5G45930.1; -.
iPTMnet; Q5XF33; -.
PaxDb; Q5XF33; -.
PRIDE; Q5XF33; -.
EnsemblPlants; AT5G45930.1; AT5G45930.1; AT5G45930.
GeneID; 834633; -.
Gramene; AT5G45930.1; AT5G45930.1; AT5G45930.
KEGG; ath:AT5G45930; -.
Araport; AT5G45930; -.
TAIR; locus:2152405; AT5G45930.
eggNOG; ENOG410IESJ; Eukaryota.
eggNOG; COG1239; LUCA.
HOGENOM; HOG000225091; -.
InParanoid; Q5XF33; -.
KO; K03405; -.
OMA; ILVTEKF; -.
OrthoDB; EOG09360D7Y; -.
PhylomeDB; Q5XF33; -.
BioCyc; ARA:AT5G45930-MONOMER; -.
UniPathway; UPA00668; -.
PRO; PR:Q5XF33; -.
Proteomes; UP000006548; Chromosome 5.
ExpressionAtlas; Q5XF33; baseline and differential.
Genevisible; Q5XF33; AT.
GO; GO:0009507; C:chloroplast; IDA:TAIR.
GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
GO; GO:0010007; C:magnesium chelatase complex; TAS:TAIR.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0016887; F:ATPase activity; IDA:TAIR.
GO; GO:0016851; F:magnesium chelatase activity; ISS:TAIR.
GO; GO:0015995; P:chlorophyll biosynthetic process; IMP:TAIR.
GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
GO; GO:0009735; P:response to cytokinin; IDA:TAIR.
InterPro; IPR003593; AAA+_ATPase.
InterPro; IPR011775; Mg_chelatase_ATPase-isu.
InterPro; IPR000523; Mg_chelatse_chII_dom.
InterPro; IPR027417; P-loop_NTPase.
Pfam; PF01078; Mg_chelatase; 1.
SMART; SM00382; AAA; 1.
SUPFAM; SSF52540; SSF52540; 1.
TIGRFAMs; TIGR02030; BchI-ChlI; 1.
1: Evidence at protein level;
Acetylation; ATP-binding; Chlorophyll biosynthesis; Chloroplast;
Complete proteome; Disulfide bond; Ligase; Nucleotide-binding;
Photosynthesis; Plastid; Reference proteome; Transit peptide.
TRANSIT 1 55 Chloroplast.
{ECO:0000244|PubMed:22223895,
ECO:0000255}.
CHAIN 56 418 Magnesium-chelatase subunit ChlI-2,
chloroplastic.
/FTId=PRO_0000418770.
NP_BIND 113 120 ATP. {ECO:0000255}.
MOD_RES 56 56 N-acetylvaline.
{ECO:0000244|PubMed:22223895}.
DISULFID 96 187 {ECO:0000250}.
DISULFID 348 390 Inhibitory under oxidizing conditions.
{ECO:0000250}.
CONFLICT 371 371 A -> T (in Ref. 3; AAM98163).
{ECO:0000305}.
SEQUENCE 418 AA; 46098 MW; FC3ECB5A0D406C9D CRC64;
MASLLGRSPS SILTCPRISS PSSTSSMSHL CFGPEKLSGR IQFNPKKNRS RYHVSVMNVA
TEINSVEQAK KIDSKESARP VYPFAAIVGQ DEMKLCLLLN VIDPKIGGVM IMGDRGTGKS
TTVRSLVDLL PEITVVSGDP YNSDPRDPEC MGKEVREKVQ KGEELSVIET KINMVDLPLG
ATEDRVCGTI DIEKALTEGV KAFEPGLLAK ANRGILYVDE VNLLDDHLVD VLLDSAASGW
NTVEREGISI SHPARFILIG SGNPEEGELR PQLLDRFGMH AQVGTVRDAE LRVKIVEERA
RFDSNPKEFR ETYQEEQLKL QEQITTARSN LSAVQIDQDL KVKISKVCAE LDVDGLRGDM
VINRAARALA ALQGRDQVTA EDVGIVIPNC LRHRLRKDPL ESMDSGILVT EKFYEVFT


Related products :

Catalog number Product name Quantity
EIAAB34361 A1 37 kDa subunit,Activator 1 37 kDa subunit,Activator 1 subunit 4,Homo sapiens,Human,Replication factor C 37 kDa subunit,Replication factor C subunit 4,RF-C 37 kDa subunit,RFC37,RFC4
EIAAB34360 A1 38 kDa subunit,Activator 1 38 kDa subunit,Activator 1 subunit 3,Homo sapiens,Human,Replication factor C 38 kDa subunit,Replication factor C subunit 3,RF-C 38 kDa subunit,RFC3,RFC38
EIAAB34355 A1 40 kDa subunit,Activator 1 40 kDa subunit,Activator 1 subunit 2,Homo sapiens,Human,Replication factor C 40 kDa subunit,Replication factor C subunit 2,RF-C 40 kDa subunit,RFC2,RFC40
EIAAB34363 A1 36 kDa subunit,Activator 1 36 kDa subunit,Activator 1 subunit 5,Homo sapiens,Human,Replication factor C 36 kDa subunit,Replication factor C subunit 5,RF-C 36 kDa subunit,RFC36,RFC5
EIAAB29611 Homo sapiens,Human,PAF acetylhydrolase 30 kDa subunit,PAF-AH 30 kDa subunit,PAFAH subunit beta,PAF-AH subunit beta,PAFAH1B2,PAFAHB,Platelet-activating factor acetylhydrolase IB subunit beta
EIAAB29619 Homo sapiens,Human,PAF acetylhydrolase 29 kDa subunit,PAF-AH 29 kDa subunit,PAFAH subunit gamma,PAF-AH subunit gamma,PAFAH1B3,PAFAHG,Platelet-activating factor acetylhydrolase IB subunit gamma
EIAAB29618 Mouse,Mus musculus,PAF acetylhydrolase 29 kDa subunit,PAF-AH 29 kDa subunit,PAFAH subunit gamma,PAF-AH subunit gamma,Pafah1b3,Pafahg,Platelet-activating factor acetylhydrolase IB subunit gamma
EIAAB29617 Bos taurus,Bovine,PAF acetylhydrolase 29 kDa subunit,PAF-AH 29 kDa subunit,PAFAH subunit gamma,PAF-AH subunit gamma,PAFAH1B3,PAFAHG,Platelet-activating factor acetylhydrolase IB subunit gamma
EIAAB29614 Bos taurus,Bovine,PAF acetylhydrolase 30 kDa subunit,PAF-AH 30 kDa subunit,PAFAH subunit beta,PAF-AH subunit beta,PAFAH1B2,PAFAHB,Platelet-activating factor acetylhydrolase IB subunit beta
EIAAB29612 Mouse,Mus musculus,PAF acetylhydrolase 30 kDa subunit,PAF-AH 30 kDa subunit,PAFAH subunit beta,PAF-AH subunit beta,Pafah1b2,Pafahb,Platelet-activating factor acetylhydrolase IB subunit beta
EIAAB34354 A1 40 kDa subunit,Activator 1 40 kDa subunit,Activator 1 subunit 2,Mouse,Mus musculus,Replication factor C 40 kDa subunit,Replication factor C subunit 2,RF-C 40 kDa subunit,Rfc2,RFC40
EIAAB34364 A1 36 kDa subunit,Activator 1 36 kDa subunit,Activator 1 subunit 5,Mouse,Mus musculus,Replication factor C 36 kDa subunit,Replication factor C subunit 5,RF-C 36 kDa subunit,RFC36,Rfc5
EIAAB34359 A1 38 kDa subunit,Activator 1 38 kDa subunit,Activator 1 subunit 3,Mouse,Mus musculus,Replication factor C 38 kDa subunit,Replication factor C subunit 3,RF-C 38 kDa subunit,Rfc3,RFC38
EIAAB34358 A1 38 kDa subunit,Activator 1 38 kDa subunit,Activator 1 subunit 3,Bos taurus,Bovine,Replication factor C 38 kDa subunit,Replication factor C subunit 3,RF-C 38 kDa subunit,RFC3,RFC38
U1345m CLIA Epiligrin subunit alpha,Kalinin subunit alpha,Lama3,Laminin subunit alpha-3,Laminin-5 subunit alpha,Laminin-6 subunit alpha,Laminin-7 subunit alpha,Mouse,Mus musculus,Nicein subunit alpha 96T
E1345m ELISA kit Epiligrin subunit alpha,Kalinin subunit alpha,Lama3,Laminin subunit alpha-3,Laminin-5 subunit alpha,Laminin-6 subunit alpha,Laminin-7 subunit alpha,Mouse,Mus musculus,Nicein subunit alpha 96T
E1345m ELISA Epiligrin subunit alpha,Kalinin subunit alpha,Lama3,Laminin subunit alpha-3,Laminin-5 subunit alpha,Laminin-6 subunit alpha,Laminin-7 subunit alpha,Mouse,Mus musculus,Nicein subunit alpha 96T
U1345h CLIA E170,Epiligrin 170 kDa subunit,Epiligrin subunit alpha,Homo sapiens,Human,Kalinin subunit alpha,LAMA3,Laminin subunit alpha-3,Laminin-5 subunit alpha,Laminin-6 subunit alpha,Laminin-7 subunit alp 96T
E1345h ELISA E170,Epiligrin 170 kDa subunit,Epiligrin subunit alpha,Homo sapiens,Human,Kalinin subunit alpha,LAMA3,Laminin subunit alpha-3,Laminin-5 subunit alpha,Laminin-6 subunit alpha,Laminin-7 subunit al 96T
20-272-191788 RFC1 - Mouse monoclonal [1320] to RFC1; Replication factor C large subunit; RF-C 140 kDa subunit; Activator 1 140 kDa subunit; Activator 1 large subunit; A1 140 kDa subunit; DNA-binding protein PO-GA 0.05 mg
EIAAB32718 HC9,Homo sapiens,Human,Macropain subunit C9,Multicatalytic endopeptidase complex subunit C9,Proteasome component C9,Proteasome subunit alpha type-4,Proteasome subunit L,PSC9,PSMA4
EIAAB32715 Macropain subunit C9,Mouse,Multicatalytic endopeptidase complex subunit C9,Mus musculus,Proteasome component C9,Proteasome subunit alpha type-4,Proteasome subunit L,Psma4
EIAAB32714 Macropain subunit C8,Mouse,Multicatalytic endopeptidase complex subunit C8,Mus musculus,Proteasome component C8,Proteasome subunit alpha type-3,Proteasome subunit K,Psma3
EIAAB32716 Macropain subunit C9,Multicatalytic endopeptidase complex subunit C9,Proteasome component C9,Proteasome subunit alpha type-4,Proteasome subunit L,Psma4,Rat,Rattus norvegicus
EIAAB32711 Macropain subunit C8,Multicatalytic endopeptidase complex subunit C8,Proteasome component C8,Proteasome subunit alpha type-3,Proteasome subunit K,Psma3,Rat,Rattus norvegicus


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur