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Mannonate dehydratase (EC 4.2.1.8) (D-mannonate hydro-lyase)

 UXUA_ECOLI              Reviewed;         394 AA.
P24215; O87739; Q2M5Z1;
01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
01-FEB-1995, sequence version 2.
30-AUG-2017, entry version 136.
RecName: Full=Mannonate dehydratase;
EC=4.2.1.8;
AltName: Full=D-mannonate hydro-lyase;
Name=uxuA; OrderedLocusNames=b4322, JW4285;
Escherichia coli (strain K12).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=83333;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
Mizobuchi K.;
Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=7610040; DOI=10.1093/nar/23.12.2105;
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.,
Blattner F.R.;
"Analysis of the Escherichia coli genome VI: DNA sequence of the
region from 92.8 through 100 minutes.";
Nucleic Acids Res. 23:2105-2119(1995).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=9278503; DOI=10.1126/science.277.5331.1453;
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1462(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=16738553; DOI=10.1038/msb4100049;
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains
MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-128.
STRAIN=O2:K1:H+ / MT78;
PubMed=9766199; DOI=10.1016/S0923-2508(98)80002-8;
Marc D., Arne P., Bree A., Dho-Moulin M.;
"Colonization ability and pathogenic properties of a fim- mutant of an
avian strain of Escherichia coli.";
Res. Microbiol. 149:473-485(1998).
[6]
PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
STRAIN=K12;
PubMed=3083215; DOI=10.1007/BF00330526;
Blanco C., Ritzenthaler P., Kolb A.;
"The regulatory region of the uxuAB operon in Escherichia coli K12.";
Mol. Gen. Genet. 202:112-119(1986).
[7]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
STRAIN=K12;
PubMed=8550444; DOI=10.1128/jb.178.1.61-67.1996;
Klemm P., Tong S., Nielsen H., Conway T.;
"The gntP gene of Escherichia coli involved in gluconate uptake.";
J. Bacteriol. 178:61-67(1996).
[8]
FUNCTION, CATALYTIC ACTIVITY, COFACTOR, PH DEPENDENCE, AND ENZYME
REGULATION.
STRAIN=K12;
PubMed=3038546; DOI=10.1111/j.1432-1033.1987.tb13559.x;
Dreyer J.L.;
"The role of iron in the activation of mannonic and altronic acid
hydratases, two Fe-requiring hydro-lyases.";
Eur. J. Biochem. 166:623-630(1987).
-!- FUNCTION: Catalyzes the dehydration of D-mannonate.
{ECO:0000269|PubMed:3038546}.
-!- CATALYTIC ACTIVITY: D-mannonate = 2-dehydro-3-deoxy-D-gluconate +
H(2)O. {ECO:0000269|PubMed:3038546}.
-!- COFACTOR:
Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
Evidence={ECO:0000269|PubMed:3038546};
Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
Evidence={ECO:0000269|PubMed:3038546};
Note=Mn(2+) can substitute for iron, but in higher concentrations.
Cannot use Fe(3+), Ni(2+) or Mg(2+). {ECO:0000269|PubMed:3038546};
-!- ENZYME REGULATION: Is inhibited by high concentrations of Fe(2+)
(> 2 mM), and by EDTA or other iron chelators in vitro.
{ECO:0000269|PubMed:3038546}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
pH dependence:
Optimum pH is about 7.5. The activity at pH 6.5 and 8.6 is only
about 20% of the maximal achievable activity at optimum pH.
{ECO:0000269|PubMed:3038546};
-!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
interconversion.
-!- INDUCTION: By fructuronate. Its expression is subjected to
catabolite repression by glucose.
-!- SIMILARITY: Belongs to the mannonate dehydratase family.
{ECO:0000305}.
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EMBL; D13329; BAA02590.1; -; Genomic_DNA.
EMBL; U14003; AAA97218.1; -; Genomic_DNA.
EMBL; U00096; AAC77278.1; -; Genomic_DNA.
EMBL; AP009048; BAE78315.1; -; Genomic_DNA.
EMBL; AJ225176; CAA12424.1; -; Genomic_DNA.
EMBL; X03411; CAA27147.1; ALT_TERM; Genomic_DNA.
EMBL; X91735; CAA62860.1; -; Genomic_DNA.
PIR; I57745; I57745.
PIR; S56547; S56547.
RefSeq; NP_418742.1; NC_000913.3.
RefSeq; WP_000438562.1; NZ_LN832404.1.
PDB; 4EAC; X-ray; 2.30 A; A/B/C/D=1-394.
PDB; 4EAY; X-ray; 2.35 A; A/B/C/D=1-394.
PDBsum; 4EAC; -.
PDBsum; 4EAY; -.
ProteinModelPortal; P24215; -.
SMR; P24215; -.
BioGrid; 4261003; 10.
DIP; DIP-11108N; -.
IntAct; P24215; 2.
STRING; 316407.85677065; -.
PaxDb; P24215; -.
PRIDE; P24215; -.
EnsemblBacteria; AAC77278; AAC77278; b4322.
EnsemblBacteria; BAE78315; BAE78315; BAE78315.
GeneID; 947082; -.
KEGG; ecj:JW4285; -.
KEGG; eco:b4322; -.
PATRIC; fig|1411691.4.peg.2370; -.
EchoBASE; EB1059; -.
EcoGene; EG11066; uxuA.
eggNOG; ENOG4105CHZ; Bacteria.
eggNOG; COG1312; LUCA.
HOGENOM; HOG000195381; -.
InParanoid; P24215; -.
KO; K01686; -.
PhylomeDB; P24215; -.
BioCyc; EcoCyc:MANNONDEHYDRAT-MONOMER; -.
BioCyc; MetaCyc:MANNONDEHYDRAT-MONOMER; -.
UniPathway; UPA00246; -.
PRO; PR:P24215; -.
Proteomes; UP000000318; Chromosome.
Proteomes; UP000000625; Chromosome.
GO; GO:0008198; F:ferrous iron binding; IDA:EcoCyc.
GO; GO:0030145; F:manganese ion binding; IDA:EcoCyc.
GO; GO:0008927; F:mannonate dehydratase activity; IDA:EcoCyc.
GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
GO; GO:0042840; P:D-glucuronate catabolic process; IMP:EcoCyc.
HAMAP; MF_00106; UxuA; 1.
InterPro; IPR004628; Man_deHydtase.
InterPro; IPR013022; Xyl_isomerase-like_TIM-brl.
PANTHER; PTHR30387:SF3; PTHR30387:SF3; 1.
Pfam; PF03786; UxuA; 1.
PIRSF; PIRSF016049; Man_dehyd; 1.
SUPFAM; SSF51658; SSF51658; 2.
TIGRFAMs; TIGR00695; uxuA; 1.
1: Evidence at protein level;
3D-structure; Complete proteome; Iron; Lyase; Manganese;
Reference proteome.
CHAIN 1 394 Mannonate dehydratase.
/FTId=PRO_0000170670.
VARIANT 52 52 I -> V (in strain: MT78).
STRAND 3 6 {ECO:0000244|PDB:4EAC}.
HELIX 16 22 {ECO:0000244|PDB:4EAC}.
STRAND 26 29 {ECO:0000244|PDB:4EAC}.
HELIX 42 54 {ECO:0000244|PDB:4EAC}.
STRAND 58 63 {ECO:0000244|PDB:4EAC}.
HELIX 69 73 {ECO:0000244|PDB:4EAC}.
HELIX 78 94 {ECO:0000244|PDB:4EAC}.
STRAND 99 102 {ECO:0000244|PDB:4EAC}.
STRAND 113 118 {ECO:0000244|PDB:4EAC}.
STRAND 124 128 {ECO:0000244|PDB:4EAC}.
HELIX 130 139 {ECO:0000244|PDB:4EAC}.
HELIX 146 149 {ECO:0000244|PDB:4EAC}.
HELIX 152 163 {ECO:0000244|PDB:4EAC}.
HELIX 167 178 {ECO:0000244|PDB:4EAC}.
HELIX 190 198 {ECO:0000244|PDB:4EAC}.
TURN 199 202 {ECO:0000244|PDB:4EAC}.
HELIX 205 226 {ECO:0000244|PDB:4EAC}.
STRAND 229 232 {ECO:0000244|PDB:4EAC}.
STRAND 236 239 {ECO:0000244|PDB:4EAC}.
STRAND 248 250 {ECO:0000244|PDB:4EAC}.
HELIX 251 260 {ECO:0000244|PDB:4EAC}.
STRAND 266 271 {ECO:0000244|PDB:4EAC}.
HELIX 272 275 {ECO:0000244|PDB:4EAC}.
HELIX 283 290 {ECO:0000244|PDB:4EAC}.
HELIX 291 293 {ECO:0000244|PDB:4EAC}.
STRAND 294 298 {ECO:0000244|PDB:4EAC}.
STRAND 302 304 {ECO:0000244|PDB:4EAC}.
STRAND 311 313 {ECO:0000244|PDB:4EAC}.
STRAND 318 321 {ECO:0000244|PDB:4EAC}.
HELIX 323 339 {ECO:0000244|PDB:4EAC}.
HELIX 357 361 {ECO:0000244|PDB:4EAC}.
HELIX 371 391 {ECO:0000244|PDB:4EAC}.
SEQUENCE 394 AA; 44838 MW; 0716A50BD6436679 CRC64;
MEQTWRWYGP NDPVSLADVR QAGATGVVTA LHHIPNGEVW SVEEILKRKA IIEDAGLVWS
VVESVPIHED IKTHTGNYEQ WIANYQQTLR NLAQCGIRTV CYNFMPVLDW TRTDLEYVLP
DGSKALRFDQ IEFAAFEMHI LKRPGAEADY TEEEIAQAAE RFATMSDEDK ARLTRNIIAG
LPGAEEGYTL DQFRKHLELY KDIDKAKLRE NFAVFLKAII PVAEEVGVRM AVHPDDPPRP
ILGLPRIVST IEDMQWMVDT VNSMANGFTM CTGSYGVRAD NDLVDMIKQF GPRIYFTHLR
STMREDNPKT FHEAAHLNGD VDMYEVVKAI VEEEHRRKAE GKEDLIPMRP DHGHQMLDDL
KKKTNPGYSA IGRLKGLAEV RGVELAIQRA FFSR


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