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Mediator of RNA polymerase II transcription subunit 25 (AtMED25) (Phytochrome and flowering time 1 protein) (Phytochrome and flowering time regulatory protein 1)

 MED25_ARATH             Reviewed;         836 AA.
Q7XYY2; F4ICJ2; Q9C6M3;
11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
01-OCT-2003, sequence version 1.
25-OCT-2017, entry version 98.
RecName: Full=Mediator of RNA polymerase II transcription subunit 25;
Short=AtMED25;
AltName: Full=Phytochrome and flowering time 1 protein;
AltName: Full=Phytochrome and flowering time regulatory protein 1;
Name=MED25; Synonyms=MED25_1, PFT1; OrderedLocusNames=At1g25540;
ORFNames=F2J7.4;
Arabidopsis thaliana (Mouse-ear cress).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
Arabidopsis.
NCBI_TaxID=3702;
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION
PHENOTYPE, AND SUBCELLULAR LOCATION.
PubMed=12815435; DOI=10.1038/nature01636;
Cerdan P.D., Chory J.;
"Regulation of flowering time by light quality.";
Nature 423:881-885(2003).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
PubMed=11130712; DOI=10.1038/35048500;
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis
thaliana.";
Nature 408:816-820(2000).
[3]
GENOME REANNOTATION.
STRAIN=cv. Columbia;
The Arabidopsis Information Portal (Araport);
Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
[4]
INDUCTION BY METHYL JASMONATE AND FUNGAL PATHOGEN.
PubMed=16183832; DOI=10.1104/pp.105.068544;
McGrath K.C., Dombrecht B., Manners J.M., Schenk P.M., Edgar C.I.,
Maclean D.J., Scheible W.R., Udvardi M.K., Kazan K.;
"Repressor- and activator-type ethylene response factors functioning
in jasmonate signaling and disease resistance identified via a genome-
wide screen of Arabidopsis transcription factor gene expression.";
Plant Physiol. 139:949-959(2005).
[5]
FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE
MEDIATOR COMPLEX, AND NOMENCLATURE.
PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
"Purification of a plant mediator from Arabidopsis thaliana identifies
PFT1 as the Med25 subunit.";
Mol. Cell 26:717-729(2007).
[6]
FUNCTION.
PubMed=18790998; DOI=10.1104/pp.108.125468;
Wollenberg A.C., Strasser B., Cerdan P.D., Amasino R.M.;
"Acceleration of flowering during shade avoidance in Arabidopsis
alters the balance between FLOWERING LOCUS C-mediated repression and
photoperiodic induction of flowering.";
Plant Physiol. 148:1681-1694(2008).
[7]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=19671879; DOI=10.1105/tpc.109.066910;
Kidd B.N., Edgar C.I., Kumar K.K., Aitken E.A., Schenk P.M.,
Manners J.M., Kazan K.;
"The mediator complex subunit PFT1 is a key regulator of jasmonate-
dependent defense in Arabidopsis.";
Plant Cell 21:2237-2252(2009).
[8]
INTERACTION WITH BBX20; PHL1; RAP2-2; ERF1B; ERF091; ERF095; ERF098
AND ERF109.
PubMed=21343311; DOI=10.1093/mp/ssr002;
Ou B., Yin K.Q., Liu S.N., Yang Y., Gu T., Wing Hui J.M., Zhang L.,
Miao J., Kondou Y., Matsui M., Gu H.Y., Qu L.J.;
"A high-throughput screening system for Arabidopsis transcription
factors and its application to Med25-dependent transcriptional
regulation.";
Mol. Plant 4:546-555(2011).
[9]
IDENTIFICATION, AND NOMENCLATURE.
PubMed=22021418; DOI=10.1104/pp.111.188300;
Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
"The Mediator complex in plants: structure, phylogeny, and expression
profiling of representative genes in a dicot (Arabidopsis) and a
monocot (rice) during reproduction and abiotic stress.";
Plant Physiol. 157:1609-1627(2011).
[10]
FUNCTION, INTERACTION WITH HB29; PHL1 AND DREB2A, AND DISRUPTION
PHENOTYPE.
PubMed=21536906; DOI=10.1073/pnas.1002981108;
Elfving N., Davoine C., Benlloch R., Blomberg J., Braennstroem K.,
Mueller D., Nilsson A., Ulfstedt M., Ronne H., Wingsle G., Nilsson O.,
Bjoerklund S.;
"The Arabidopsis thaliana Med25 mediator subunit integrates
environmental cues to control plant development.";
Proc. Natl. Acad. Sci. U.S.A. 108:8245-8250(2011).
[11]
FUNCTION.
PubMed=21985558; DOI=10.1111/j.1365-313X.2011.04815.x;
Inigo S., Alvarez M.J., Strasser B., Califano A., Cerdan P.D.;
"PFT1, the MED25 subunit of the plant Mediator complex, promotes
flowering through CONSTANS dependent and independent mechanisms in
Arabidopsis.";
Plant J. 69:601-612(2012).
[12]
INTERACTION WITH DREB2A.
PubMed=22447446; DOI=10.1093/nar/gks265;
Blomberg J., Aguilar X., Braennstroem K., Rautio L., Olofsson A.,
Wittung-Stafshede P., Bjoerklund S.;
"Interactions between DNA, transcriptional regulator Dreb2a and the
Med25 mediator subunit from Arabidopsis thaliana involve
conformational changes.";
Nucleic Acids Res. 40:5938-5950(2012).
[13]
INTERACTION WITH ABI5 AND MYC2.
PubMed=22822206; DOI=10.1105/tpc.112.098277;
Chen R., Jiang H., Li L., Zhai Q., Qi L., Zhou W., Liu X., Li H.,
Zheng W., Sun J., Li C.;
"The Arabidopsis mediator subunit MED25 differentially regulates
jasmonate and abscisic acid signaling through interacting with the
MYC2 and ABI5 transcription factors.";
Plant Cell 24:2898-2916(2012).
[14]
INTERACTION WITH MBR1 AND MBR2.
PubMed=22992513; DOI=10.1104/pp.112.205500;
Inigo S., Giraldez A.N., Chory J., Cerdan P.D.;
"Proteasome-mediated turnover of Arabidopsis MED25 is coupled to the
activation of FLOWERING LOCUS T transcription.";
Plant Physiol. 160:1662-1673(2012).
-!- FUNCTION: Component of the Mediator complex, a coactivator
involved in the regulated transcription of nearly all RNA
polymerase II-dependent genes. Mediator functions as a bridge to
convey information from gene-specific regulatory proteins to the
basal RNA polymerase II transcription machinery. Mediator is
recruited to promoters by direct interactions with regulatory
proteins and serves as a scaffold for the assembly of a functional
preinitiation complex with RNA polymerase II and the general
transcription factors. Positive regulator of shade avoidance and
of jasmonate signaling. Acts in repression of PhyB-mediated light
signaling and regulates the expression of FLOWERING LOCUS T (FT)
and of CONSTANS (CO). {ECO:0000269|PubMed:12815435,
ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:18790998,
ECO:0000269|PubMed:19671879, ECO:0000269|PubMed:21536906,
ECO:0000269|PubMed:21985558}.
-!- SUBUNIT: Component of the Mediator complex. Interacts with the
transcription factors BBX20, RAP2-2, ERF1B, ERF091, ERF095,
ERF098, ERF109, HB29, PHL1, DREB2A, ABI5 and MYC2. Interacts with
the E3 ubiquitin-protein ligases MBR1 and MBR2.
{ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:21343311,
ECO:0000269|PubMed:21536906, ECO:0000269|PubMed:22447446,
ECO:0000269|PubMed:22822206, ECO:0000269|PubMed:22992513}.
-!- INTERACTION:
O82132:DREB2A; NbExp=3; IntAct=EBI-15924435, EBI-1786840;
Q9FIP9:MYC3; NbExp=2; IntAct=EBI-15924435, EBI-15845995;
Q8GUN5-2:PHL1; NbExp=2; IntAct=EBI-15924435, EBI-15924466;
P06492:UL48 (xeno); NbExp=2; IntAct=EBI-1386292, EBI-7489933;
Q9SEZ1:ZHD11; NbExp=3; IntAct=EBI-15924435, EBI-1806317;
-!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12815435}.
-!- ALTERNATIVE PRODUCTS:
Event=Alternative splicing; Named isoforms=2;
Name=1;
IsoId=Q7XYY2-1; Sequence=Displayed;
Name=2;
IsoId=Q7XYY2-2; Sequence=VSP_043993;
Note=Derived from EST data. No experimental confirmation
available.;
-!- INDUCTION: Down-regulated by methyl jasmonate and by infection
with an incompatible isolate of a necrotrophic fungal pathogen.
{ECO:0000269|PubMed:16183832}.
-!- DISRUPTION PHENOTYPE: Late-flowering phenotype when grown under
long-day conditions. Increased drought tolerance and sensitivity
to necrotrophic pathogens. Increased resistance to hemibiotrophic
fungal pathogen F.oxysporum. {ECO:0000269|PubMed:12815435,
ECO:0000269|PubMed:19671879, ECO:0000269|PubMed:21536906}.
-!- MISCELLANEOUS: The affinity between DREB2A and MED25 is reduced
when DREB2A is pre-bound to DNA.
-!- SIMILARITY: Belongs to the Mediator complex subunit 25 family.
{ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=AAG50810.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; AY170377; AAO39425.1; -; mRNA.
EMBL; AC079281; AAG50810.1; ALT_SEQ; Genomic_DNA.
EMBL; CP002684; AEE30639.1; -; Genomic_DNA.
EMBL; CP002684; AEE30640.1; -; Genomic_DNA.
PIR; G86385; G86385.
RefSeq; NP_001077596.1; NM_001084127.1. [Q7XYY2-2]
RefSeq; NP_173925.3; NM_102365.5. [Q7XYY2-1]
UniGene; At.17319; -.
ProteinModelPortal; Q7XYY2; -.
BioGrid; 24378; 27.
DIP; DIP-39923N; -.
IntAct; Q7XYY2; 6.
STRING; 3702.AT1G25540.1; -.
iPTMnet; Q7XYY2; -.
PaxDb; Q7XYY2; -.
PRIDE; Q7XYY2; -.
EnsemblPlants; AT1G25540.1; AT1G25540.1; AT1G25540. [Q7XYY2-1]
EnsemblPlants; AT1G25540.2; AT1G25540.2; AT1G25540. [Q7XYY2-2]
GeneID; 839141; -.
Gramene; AT1G25540.1; AT1G25540.1; AT1G25540.
Gramene; AT1G25540.2; AT1G25540.2; AT1G25540.
KEGG; ath:AT1G25540; -.
Araport; AT1G25540; -.
TAIR; locus:2031200; AT1G25540.
eggNOG; ENOG410JR23; Eukaryota.
eggNOG; ENOG410ZJYF; LUCA.
HOGENOM; HOG000030719; -.
InParanoid; Q7XYY2; -.
KO; K15168; -.
OMA; TGMGQQF; -.
OrthoDB; EOG0936047Z; -.
PhylomeDB; Q7XYY2; -.
PRO; PR:Q7XYY2; -.
Proteomes; UP000006548; Chromosome 1.
Genevisible; Q7XYY2; AT.
GO; GO:0016592; C:mediator complex; IDA:TAIR.
GO; GO:0005634; C:nucleus; IDA:TAIR.
GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
GO; GO:0003713; F:transcription coactivator activity; NAS:TAIR.
GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IMP:TAIR.
GO; GO:0031349; P:positive regulation of defense response; IMP:TAIR.
GO; GO:0009911; P:positive regulation of flower development; IMP:TAIR.
GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:TAIR.
GO; GO:0009585; P:red, far-red light phototransduction; IMP:TAIR.
GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
GO; GO:0010218; P:response to far red light; IMP:TAIR.
GO; GO:0010114; P:response to red light; IMP:TAIR.
GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
InterPro; IPR021419; Mediator_Med25_VWA.
Pfam; PF11265; Med25_VWA; 1.
1: Evidence at protein level;
Alternative splicing; Coiled coil; Complete proteome; Flowering;
Jasmonic acid signaling pathway; Nucleus; Reference proteome;
Transcription; Transcription regulation.
CHAIN 1 836 Mediator of RNA polymerase II
transcription subunit 25.
/FTId=PRO_0000418124.
COILED 680 761 {ECO:0000255}.
COMPBIAS 92 95 Poly-Gly.
COMPBIAS 677 826 Gln-rich.
COMPBIAS 821 825 Poly-Gly.
VAR_SEQ 1 109 Missing (in isoform 2). {ECO:0000305}.
/FTId=VSP_043993.
SEQUENCE 836 AA; 89566 MW; 9A9C81527BE4F384 CRC64;
MSSEVKQLIV VAEGTAALGP YWQTIVSDYL EKIIRSFCGS ELNGERNPVS TVELSLVIFN
SHGSYCACLV QRSGWTRDVD IFLHWLSSIQ FGGGGFNEVA TAEGLAEALM MFSPPSGQAQ
PSNDLKRHCI LITASNPHIL PTPVYRPRLQ NVERNENGDA QAESRLSDAE TVASYFAKCS
VSLSVVCPKQ LPTIRALYNA GKPNQQSADL SIDTAKNTFY LVLISENFVE ACAALSHSAT
NLPQTQSPVK VDRATVAPSI PVTGQPPAPV SSANGPIQNR QPVSVGPVPT ATVKVEPSTV
TSMAPVPSFP HIPAVARPAT QAIPSIQTSS ASPVSQDMVS NAENAPDIKP VVVSGMTPPL
RTGPPGGANV NLLNNLSQVR QVMSSAALAG AASSVGQSAV AMHMSNMIST GMATSLPPSQ
TVFSTGQQGI TSMAGSGALM GSAQTGQSPG PNNAFSPQTT SNVASNLGVS QPMQGMNQGS
HSGAMMQGGI SMNQNMMSGL GQGNVSSGTG GMMPTPGVGQ QAQSGIQQLG GSNSSAPNMQ
LSQPSSGAMQ TSQSKYVKVW EGNLSGQRQG QPVLITRLEG YRSASASDSL AANWPPTMQI
VRLISQDHMN NKQYVGKADF LVFRAMSQHG FLGQLQDKKL CAVIQLPSQT LLLSVSDKAC
RLIGMLFPGD MVVFKPQIPN QQQQQQQQLH QQQQQQQQIQ QQQQQQQHLQ QQQMPQLQQQ
QQQHQQQQQQ QHQLSQLQHH QQQQQQQQQQ QQQHQLTQLQ HHHQQQQQAS PLNQMQQQTS
PLNQMQQQTS PLNQMQQQQQ PQQMVMGGQA FAQAPGRSQQ GGGGGQPNMP GAGFMG


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